no (apparent) defect in eif2a(p) in as-rx’d hri-/- mefs
DESCRIPTION
No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs. HRI-/- #3. WT #7. HRI-/- #5. WT #11. Tg60’. Tg15’. Tg15’. Tg30’. Tg60’. Tg30’. Tg60’. Tg30’. Tg15’. Tg30’. Tg60’. Tg15’. As30’. As15’. As30’. As60’. As60’. As15’. As30’. As60’. As15’. As30’. As60’. As15’. UT. UT. - PowerPoint PPT PresentationTRANSCRIPT
No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs
UT Tg
15’
Tg30
’Tg
60’
As15
’As
30’
As60
’U
T Tg15
’Tg
30’
Tg60
’As
15’
As30
’As
60’
HRI-/- #3 WT #7
eIF2a(P)
eIF2a(T)
UT Tg15
’Tg
30’
Tg60
’As
15’
As30
’As
60’
UT Tg15
’Tg
30’
Tg60
’As
15’
As30
’As
60’
HRI-/- #5 WT #11
Isabel Novoa & Yuhong Zhang
As: Sodium arsenite, 100M,
Tg: Thapsigargin, 100nM
Genotyping of HRI locus
WT allele(P2/P6 PCR)
MUT alleleP2/P4 PCR
MUT (1.1Kb)
WT (0.6 Kb)
1.3Kb1.1Kb0.9Kb
0.6Kb
X174HaeIII
HR
I-/- #3H
RI-/- #5
WT
HR
I-/- #3H
RI-/- #5
WT