nicholas tan resume
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Boon Siang Nicholas [email protected]
19/Gf Northumberland Street, Edinburgh, United Kingdom, EH3 6LR.'̴ +44 7414977116 ' https://uk.linkedin.com/in/nicholas-tan-66ba2359
I am a highly motivated individual with a strong passion in Clinical Sciences & Development. Equipped with knowledge and technical skills in broad disciplines of science topics (Biochemistry, Molecular Biology, Stem Cell Biology, Developmental Biology and Reproduction), I am keen to utilize these skills in a translational/clinical setting in the next stage of my career.
PROFESSIONAL EXPERIENCE[2015-Current] Postdoctoral Research Fellow, MRC Centre for Regenerative Medicine, United Kingdom
Research focuses on understanding global transcription factor binding in stem cell pluripotency, differentiation and developmental processes.
Major involvement in the establishment of a novel technique in studying genome-wide transcription factor binding in mammalian cells specifically in the embryo (DamID-seq).
Granted with the University of Edinburgh Funding Challenge Award Provided technical and scientific training to Ph.D and Undergraduate students. Built stem cell awareness across the UK through participation in educational public
engagement activities.
[2013-2014] Postdoctoral Research Associate, Walter and Eliza Hall Institute of Medical Research (WEHI), Australia
Spearheaded research on the effects of endocrine disruptors in development with the use of a mouse X-inactivation embryonic stem cell model.
[2008-2014] Postgraduate Candidate, University of Melbourne, Australia Conducted world leading research in understanding the role of amino acids and amino acid
transporters in stem cell pluripotency, differentiation and embryonic development. Designed and led 5 research projects, of which 3 resulted in cited publications. Established collaborations with Merck Millipore on a live-cell RNA imaging probe. Conducted a lecture on Stem Cells and Tissue Engineering in a Masters and Undergraduate
subject. Taught in university several practical courses across multiple disciplines (Biology,
Biomedical Sciences, Techniques in Molecular Sciences, Comparative Animal Physiology and Victorian Certificate of Education (VCE) genetics workshops)
Supervised and trained several Undergraduate and Masters Students through technical and conceptualisation advice.
Awarded with 3 postgraduate scholarships from the Australian Commonwealth and University.
Obtained travel fellowships from multiple international scientific organisations.
[2012-2014] Assistant to Head of Department, Education, Ethics, Law & Community Awareness Unit, Stem Cells Australia, Australia
Key role in the maintenance of Stem Cells Australia (SCA) social media (website, Facebook page and Twitter).
Experienced in online digital platform suites such as Pegboard. Established a seminar series (Stem Cell Conversations) focusing on social and scientific
interactions between students and young scientists of SCA. Experienced in conference organisation through “Therapeutic Potential of Stem Cells
Symposium (2013)” Major role in organisation of SCA annual retreats.
Nicholas Tan ~ [email protected]
EDUCATION[2010-2014] Ph.D. in Stem Cell Biology, University of Melbourne, Australia
[2008-2010] Masters of Science, University of Melbourne, Australia Graduated with high distinctions and obtained highest thesis score in Masters Class of 2010. Pioneer graduate from Melbourne University Professional Masters Model. (Model incorporated
cross-disciplinary subjects specifically in Ethics, Business and Communications).
[2007-2008] Bachelor of Science, University of Melbourne, Australia Double major in Reproduction & Development and Molecular Biology & Biochemistry.
[2002-2005] Diploma in Biotechnology, Singapore Polytechnic, Singapore Led research in comparing hydroponic systems in the growth of Brassica oleracea cultivar
italica.
TECHNICAL SKILLSCell Culture and Manipulation
Trained in isolation and generation of primary cells from murine tissues (specifically hematopoietic and immune cells, mouse fibroblasts and pluripotent stem cells)
Experience in establishment of induced pluripotent stem cells Skilled in maintenance and differentiation of mouse and human pluripotent stem cells
(specifically to epiblast stem cells and neural stem cells) Able in immortalisation of primary cell lines (specifically hematopoietic progenitors) Highly proficient in establishment of transgenic cell lines with transfections. Able to perform colony forming assay Experienced in embryoid body formation and differentiation
Mouse Embryo Culture and Manipulation Competent in mouse embryo handling and collection from multiple developmental stages Able to perform mouse embryo dissections (isolations through oviductal flushes and uterine
horn dissections). Proficient in culturing preimplantation mouse embryos in vitro. Trained in animal handling
Molecular Biology and Biochemistry Efficient in vector design and molecular cloning (specifically in promoter selection,
translation optimisation and establishing inducible constructs) Experienced in gene targeting Extensive experience in RNA and DNA isolations and manipulations Highly proficient in real time quantitative PCR Experienced in sample and library preparation in next-generation sequencing. Able in western blotting, immunoprecipitation and immunohistochemistry. Highly experienced in radioactive uptake assays
Flow cytometry and Imaging Trained in operating flow cytometer Highly skilled in confocal microscopy with handling experience of multiple microscopes. Experienced in live-cell confocal imaging (Both cell and embryo time lapse microscopy) Able to perform live-cell RNA imaging (resulted in industrial collaboration)
ADDITIONAL INFORMATION
Nicholas Tan ~ [email protected]
AffiliationsMember of International Society for Stem Cell ResearchMember of International Society for Cell TherapyMember of Stem Cell Society SingaporeMember of Stem Cells AustraliaMember of National Stem Cell Foundation of Australia
LanguagesEnglish (fluent) and Mandarin (fluent)
Nicholas Tan ~ [email protected]