nhóm 4 acetic

8/12/2019 nhm 4 acetic

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Cho mng c v cc bn n vi bithuyt trnh ca nhm 3

Lp 53CNTP2

Ch :Ln men acetic v ng dng

GVHD:Nguyn Th Thanh Hi


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Danh sch nhm 3

1NguynThngDuy2NguynThQucDng3NguynThDim4 TThKhnh Hu(NT)5 TrnhTH Hng6NguynThTho7 inhThTho8 Hong VnTin9 PhmVnVng

10 Hong ThYn


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Ni Dung

I Khi qut ln men acetic

II Vi khun acetic1 c im ca vi khun acetic

2 Cc loi ca vi khun aceticIII.Quy trnh ln men acetic1 C ch ln men acetic

2 Cc phng php ln men acetic3 Cc yu t nh hngIV ng dng ca ln men acetic


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I Khi qut ln men

Ln men acid acetic l qu trnh oxy ho cn thnh acid acetic

nh vi khun acetic trong iu kin hiu kh.

Qu trnh ln men l qu trnh oxy ho kh sinh hc thunng lng v cc hp cht trung gian cho t bo sinh vtnhng t bo sng ch s dng nng lng di dng ho nngtng tr trong mch cacbon v c phng ra do s chuyn

electron t mc nng lng ny sang mc nng lng khc.


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II Vi khunacetic1.c im vi khun acetic

Vi khunacetic lynnglngtqu trnh oxy haethanol tothnh acidacetic trong qu trnh ln

menL nhngtrckhungramm, khng sinh bo t, hhphiukhbtbuc, xp

ihay tothnh nhngchui, thngtothnh lpmng trnbmt, c loic khnngchuynngc loi khng.


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c im vi khun acetic

C nhiu trong rau qu Ngun dinh dng

cacbon: oxy ha ruetylic hay cc rukhc thnh acid tng

ng.C kh nng sdng glucose. Ngun dinh dng

nit: s dng muiamoni hay peptone.

C hai ngng pH: pHthch hp t 5,4 n6,8. pH thch ng chos ln men t 3,5 dn

4,5.


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a, Acetobacter aceti

2.Cc loi ca vi khun acetic

L trckhunhnh

que ngn,khngsinhbo t,khngchuynngv chng c khnng lin ktvinhau thnh chuidiChng tch tc6% acid acetic

Acetobacter aceti


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b, Acetobacter pasteurianum

L trckhunngn,btmu xanh

thmviIot.Tothnh khunlcnhnnheo trn

mi trngcC khnngtcht6,2% acid acetic

Acetobacter pasteurianum


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c, Acetobacter orleanense

L trckhunkhng chuynng,c kchthctrung bnh

Khi tngnhitchng tothnh TB hnhdi hochnh sihockhng tohnhThngthychng pht trintrong dchnho

longTch lyacid acetic 9,5%


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L trc khun khngchuyn ng, bt muxanh vi iot.

Ch c th chu c vtch t ti a 4 5% axitaxetic v sau chng oxiho axit axetic to thnhCO2v H2O.

Chng c s dngtrong ngnh sn xut nc

ung ln men c ga.

d, Acetobacter xylinum

Acetobacter xylinum


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Ngoi ra cn c cc vi khunnh: AcetobacterKutzin guanum, Acetobacter schiitzenbachii,

Acetobacter curvum ,Acetobacter Suboxydan..

Acetobacter Suboxydan


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III.Quy trnh ln men acetic

1 C ch

Qu trnh ln men nhmtnhm vi khungichungl vi khunacetic ,chng oxi ha ruetylic thnh

acid acetic


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Thcchtqu trnh ny gm2phnng

vi2 enzime ca2 vi khunacetic xctc


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S hydrat ha axetaldehyt din ra phn ngloi hydro ln 2

Hydro cNADP nhnv qua cc xitocrom cachuih hpcchuyncho oxi


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Trong ln men acetic cchtdinh dngchyulcnetanol vinngtrn di 10% trong mitrng.Ngoira cn c thm mtt ng,ngunN

v chochuc


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2, Ccphngphp ln men acetic

C 2phngphp ln men acetic : chmv nhanh

Phngphp ln men chm:Cn cgilphngphp Orlean hocphngphp Php:

Dng nchoa qulm nguyn liuvigingAcetobacterorleaneuse.

ngdng:Phngphp ny dng cho hiusutthp, thigian di nhngthch hpcho cng viclmgimgia nhhocsnxutgim quy m nhtrong cc x nghipdngruli, dchng, ncquhocbia hng, ruvang kmchtlng cho ln men


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Phngphp nhanh (phngphp c) :gingvi khuncdng l Acetobacter schiitzenbachii hocAcetobacter curvum cnhimvo phoi bo. Cho dchln menchyqua cc phoi bo nhiuln, vi khunacetic tipxc vimitrngln men v oxy ha ruthnh acid. Mi trngc thchoqua lpphoi bo nhiulncho vi khuntipxc v oxy ha huhtrutrong binthnh gim(ruetylic dkhong0,1-0,2%).Nhitln men khong24-37OC. Thigian ln men khong8-10 ngy


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Cu to thng ln men pp nhanh :


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Nhngthng ln men gim


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Ngoi ra ngi ta cn dng phng php ln men chm theokiu hin i. Sau khi etanol chuyn ha thnh acid acetic thc rt bt phn ln v a dch mi vo ni ln men tiptc. Cc chngAcertobacter suboxydans t ra thch hp vi

phng php ny, chng chuyn ha ton b etanol thnh

acid acetic vi nng cn 12% trong mi trng c thmmt t glucoza v sc kh mnh. Ln men 28-32oC khong 2ngy.


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3, Cc yutnhhng

A, Nngru:

Nngrut6-15% thngtrung bnh l 13%Khi htruchng oxy ha acid acetic thnh CO2v H2O

cung cpnnglngcho qu trnh sinh trngv pht trincavi khuntrnh trnghpny, cnkimtra nngruthngxuyn, dngqu trnh ln men khi nngrucn 0,3-0,5%


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B,Nhit:

Trong snxutngita sdngnhngloi vi khunc nhitthchngcho qu trnh oxy ha ruthnh acid acetic t30-40oC


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C,Nngacid :

asqu trnh ln menbcchkhi nngacid thn8% v khinngacid acetic t12-14% th qu trnh ln men hon tonbngnghotng


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D, Oxy :

Qu trnh ln men acetic l qu trnh oxy ha khng hon ton, chnh

v vybtbucphicung cpvalngoxy oxy ha ru

thnh acid acetic. Trnh trnghpoxy ha acid acetic thnh CO2vH2O nuthaoxy hockhng oxy ha htruthnh acid acetic nuthiuoxyTrong thctsnxutcncung cp2,5m3 khng kh khi oxy ha 1kg

rukhan thnh acid acetic


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E, Mi trngdinh dng:

Nuchc ruth vi khunacetic khng thsngcnn cnbsung mtschtdinh dngkhc. Trong 100 lt ru10% cncn500g ngglucose hay tinhbtthyphn, 25g superphotphat,25g sunfat amn, ngoi ra cnbsung thm mtschtsinh trng

khc

IV d l i


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IV. ng dng ca ln men acetic1. Trong thc phm:

- Cc vi khun acid acetic c vai tr quan trng trong thcphm v sn xut nc gii kht, cng nh trongbioproduction cc ho chtcng nghip,acid acetic ngmtvai tr tch cc trong vicsnxut cc loi thcphmc

lachnv ung,nhngcngc thlm hngthcphmv ungkhc.

VD l id ti l i


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- VD: ln men acid acetic _ ln men gim

Gim l mt nhn t quan trng v c s dng rng ritrong m thc, ni tr v ngy nay gim cn c s dng

nh l mt loi du, kem lm trng da, mt tc cho phip v c s dng rng ri hn pht trin theo nhu cungy cng cao ca con ngi

+ Gim dng nu nng: cha axit axetic 4- 5% c vnng, thm du, ty mi tanh ca c, gip ti tht, cc hisn.

+ Gi i t it ti 3 4% t


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+ Gim n vi cm v rau trn: axit axetic 3- 4%, v ngt,thm thch hp cho trn rau, nc chm, n vi ph, mqung, im tm

+Loi bo v sc khe: Axit axetic khong 1- 2%, khu v khngon, mi khi lm v mt hoc hc hnh mt mi th phagim vi ng, thm lnh vo ung sng khoi, giikht, rt tt cho sc khe.

2 T t


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2. Trong y t:- Soothing cho chy nng: Dng gim trng phun vo m

b chy nng lm khi phc lng axit b mt v lm mt cho

da.

- Kim sot lng ng huyt v bnh tiu ngCc bnh nhn b tiu ng c s dng tr gim

kim sot cc triu chng ca bnh, khong 20ml gim mingy s gim ch s ng huyt ca thc phmcarbohydrate cho bnh nhn tiu ng lm gim ch s

glycemic.

S dng khng sinh: gim c s dng ph bin chng


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-S dng khng sinh: gim c s dng ph bin chngnhim trng, chng nhim trng t Hippocrates iu tr bnhho dai dng.

- Chc nng ca gim trong c th ngi: y li v tr bnhtt, iu tit kim acid dch th, duy tr tng i n nhca mi trng trong c th, gip tiu ha, li cho hpth,phng gi yu,dit khun, khng c.


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- Ngoi ra dmpha long t3%-5% nhcng ckimtrabnhung thtcung, gimcn ccoi nhmtilngmu, lm nhanh lnh vtbngv vim da, rugim

gip chnglichngvim ddy v mtngnngacc tchicathucc.

Gimcsdngnhmtchttyra, lm dung mi lm schnhaepoxy.


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3. Trong nng nghip

- Gim c th c s dng nh mt loi thuc dit c.

- Mt mt khc na nu dng cc loi gim khng ng liulng (pH) d gy ng c, h kali mu, hyperreninemia vlong xng.


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nhóm 4 acetic

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