Paci�c Biosciences is founded to commercialize Single Molecule Real Time (SMRT) sequencing

Helicos Biosciences is founded to commercialize Single Molecule Sequencing

Lynx®, credited with massively parallel sequencing, merges with Solexa® leading to the development of sequencing-by-synthesis, used in most NGS platforms

454® releases the 454 Genome Sequencer (GS20)

Complete Genomics (CG) founded to commercialize DNA nanoball sequencing technology

2003

2004

2005

2006

Next-Generation SequencingCommercial Milestones

Next-generation sequencing (NGS) has revolutionized the way we analyze diseases and commercial out�ts such as Illumina, Helicos, QIAGEN and Paci�c Biosciences have made signi�cant contributions. In addition, the launch of direct-to-consumer genetic testing solutions has dramatically changed the way consumers access genomics data. Until a few years ago, the cost of sequencing was a major bottleneck. Recent developments have reduced the cost from thousands of dollars to a couple of cents per megabase. When did these changes start? What were the changes in the commercial sector in the last 15 years?

This infographic is a timeline of the NGS commercial marketplace.

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Sample to Insight

Trademarks: QIAGEN®, Sample to Insight®, Ingenuity®, (QIAGEN Group); 23andMe® (23andMe Inc.); 454 Life Sciences® and 454 Genome Sequencer® (Roche Inc.); Ancestry® and AncestryDNA® (Ancestry Inc.); COLOR (Color Genomics Inc.); Counsyl® (Counsyl Inc.); GeneReader™ (Intelligent Bio-Systems, Inc.); Helicos Biosciences® , Helicos Genetic Analyzer® system, (Helicos Biosciences Inc); ABI®, Ion Torrent™, SOLiD System® (Thermo Fisher Scienti�c Inc.); Lynx Biosciences® (LynxBio Group); Solexa®, Genome Analyzer®, HiSeq®, NextSeq®, Illumina® (Illumina Inc); Mapmygenome® (Mapmygenome Inc.); Myriad Genetics® (Myriad Inc.);Oxford Nanopore® (Oxford Nanopore Inc.); Paci�c Biosciences®, SMRT® (Paci�c Biosciences Inc.); Positive Bioscience® (Positive Biosciences Inc.). Registered names, trademarks, etc. used in this document, even when not speci�cally marked as such, are not to be considered unprotected by law. © 2016 QIAGEN, all rights reserved

The Mouse Genome Sequencing Consortium sequences the mouse genome and determines that humans share 99% of genes with mice

The Human Genome Project Consortium completes sequencing the �rst draft of the human genome2001

2002

Gene By Gene® specialized in DNA-based ancestry and genealogy as Family Tree DNA

2000

23andMe is the �rst company to offer DNA analysis tools to help customers understand their genetic information and ancestral background

Sequencing Cost/Megabase

$5292.39

$3656.22

$2608.59

$1217.73

$884.39

$642.33

Ion TorrentTM founded to commercialize semi-conductor sequencing technology

Illumina, a developer and maker of microarrays, acquires Solexa

Life Technologies acquires Ion Torrent

ABITM releases the SOLiDTM System

Ray Wu, Columbia University scientist credited with the �rst method for determining DNA sequences, dies at 79

Solexa releases Genome Analyzer (Genome A), it‘s sequencing platform

Oxford Nanopore Technologies showcases its ultra long single molecule sequencing technology

Detection of inherited mutations for breast and ovarian cancer using targeted NGS

Helicos releases the �rst single sequencer, Helicos Genetic Analyzer System

Helicos Biosciences �les for Chapter 11 bankruptcy

QIAGEN acquires Ingenuity, a leader in the analysis and interpretation of complex biological data

Illumina introduces the HiSeq® 2000

BGI-Shenzhen acquires Complete Genomics

Fred Sanger, “Father of Genomics“ and the only British scientist to win 2 Nobel Prizes, dies at 95

Illumina introduces the HiSeq X Ten platform; delivering on their $1000/genome promise (requires 18,000 genomes are sequenced a year)

2007

2008

2009

2010

2011

2014

2012

2013

Illumina introduces the NextSeq® 500 platform, a desktop sequencer capable of sequencing whole genomes

2015

QIAGEN acquires Intelligent Bio-Systems (IBS) and enters the sequencing market

Illumina introduces the HiSeq® 2500

Association for Molecular Pathology vs. Myriad Genetics is argued in the US Supreme Court; Myriad‘s patents on BRCA1/2 are deemed invalid

QIAGEN acquires CLC bio, a leader in NGS bioinformatics

QIAGEN introduces the GeneReaderTM sequencer—based on sequencing- by-synthesis technology

2016

Roche decides to close down 454 Life Sciences operations, after acquiring them 6 years earlier

Counsyl offers DNA testing and genetic counseling advice direct to consumers

Positive Bioscience offers cancer genomic and personal genomic tests direct to consumer

Mapmygenome offers personal genomics, molecular diagnostics and DNA forensics direct to consumer

FDA launches the PrecisionFDA platform: an online, cloud-based portal for NGS assay evaluation and regulatory science exploration

QIAGEN launches QIAseqTM NGS Solutions technology

Ancestry.com launches AncestryDNA Service: an affordable DNA test combined with the world‘s largest online family history resource

$479.59

$32.33

$1.57/Mb

$0.38

$0.16

$0.07

$0.06

$0.05

$0.05

$0.03Seq Cost/Mb

Direct-to-Consumer Test Markets

Ancestry Athletic Carrier Child talent Genetic relation In�delity Matchmaking Nutrigenic Paternity

View the NGS Clinical Research Milestones Infographic

Human Genome Project completes sequence of human genome (3.3 Gb)

Color Genomics offers tests to understand the risk of hereditary cancers, direct to consumers