New Year’s Launch of Six New Technologies for Veterinary

Clinical Pathology Hematology – Maureen McCullaugh

Blood Gas Analysis – Mick Garrett

Endocrinology – Joel Eluk, Penny Furney

Flow Cytometry – Inese Berzina, Amanda Lawler, Emma O’Neill

Multiplex Immunoassay – Marianna Serra, Penny Furney

Cellomics – Margarida Domingos

Novel State-of-the-Art Biomarker Technologiesat UCD UVH

HematologyImmunology

Advia

ImmunochemistryCentaur

MSD

Guava

Cellomics

InCell

Blood Gas Analysis

Immunochemistry

RapidLab 1200

Multiparameter Hematology System: ADVIA 2120

• Biggest advance in hematology technology for a generation in sensitivity, specificity and parameters added

Applicable to other body fluids eg effusions, BALF, CSF, & BM. Novel parameters eg reticulocyte size, maturity & Hb (most sensitive measure of Fe status), of platelet size & activation, of neutrophil myeloperoxidase, & of extracellular versus intracellular Hb• Managed by Maureen McCullaugh

Sample Port: Automatic sampling with a single port for syringe and capillary samples. Complete with automatic clot detection and clearance, short sample and bubble detection. Can backflush to clear so don’t have to replace cartridge.

• Microsampler – as little as 30 uL

• Comprehensive analytic panel: blood gas (pH, pCO2, pO2); electrolytes (Na+, K+, Ca++, CI- ); Metabolic (glucose, lactate)

• Managed by Mick Garrett in out-of-hours / student lab

RapidLab 1200: Blood Gas Analyser

• latest model in market place

• delivers results for all parameters in 1 min

• maintenance-free, cartridge-based system

• LIMS interfaceable

• Numerous Parameters• Thyroid T4, T3, TSH• Reproductive eg progesterone, estradiol, testosterone• Adrenal eg Cortisol (dog)• Cardiac eg troponin, CK-MB (dog, horse)• Vit B12 and folate

• Insulin• Skeletal Muscle troponin

• Managed by Joel Eluk and Penny Furney

Centaur CP• automated Immunoanalyzer

• latest model in market place

• replaces Immulite

Guava – Personal Flow Cytometer

• smallest and easiest to use in marketplace

• 2-angle scatter, 4 color

• being developed for use for immunophenotyping of lymphoma and leukemia

• Assessment of cell viability

• Managed by Inese Berzina, Amanda Lawler, and Emma O’Neill

• Managed by Marianna Serra, Penny Furney

MSD 2400• automated mulitplexing immunoanalyzer

• latest model in market place

• for high throughputting, needing microvolume testing (25 uL vs 150 uL), needing mutliplexing

• assay development possible• panels of mulitple cytokines and chemokines

• cardiac panel of biomarkers

• reproductive hormones

♦ Automated microscopy ♦ 96-well plate in incubator♦ Live-cell, image analysis ♦ Single cell, populations, well l♦ Real-time, kinetic, multiplex ♦ Intracellular location♦ Structure & function ♦ Managed by Margarida Domingos

High Content Analysis & Hormesis