new products 2013v2
DESCRIPTION
New products: ANTIBODIES, ASSAYS, SMALL MOLECULES, INHIBITORS, AND PROTEINSTRANSCRIPT
EMD Millipore—with the expertise of Calbiochem®, Chemicon®, and Upstate®
EMD Millipore is a division of Merck KGaA, Darmstadt, Germany
Magna ChIP® HiSens KitsExcellent sensitivity from large or small chromatin amounts These kits combine a complete set of validated reagents with a detailed protocol to create a streamlined, high performance ChIP procedure that allows the use of the same kit for a variety of experimental conditions.
Magna ChIP® HiSens Advantages• ChIP from a wide range of input amounts (10,000 to 1,000,000 cells)• Blended protein A/G magnetic beads enables the use of more antibody
isotypes. Read more at: www.emdmillipore.com/HiSens
Shaping epigenetics discovery for new or experienced ChIPpers The ChIP protocol is a fundamental tool that has yielded deep insights into epigenetic phenomena. Yet, the technique remains laborious and technically challenging. We have developed a protocol for optimizing the parameters of chromatin fragmentation and preparation for researchers new to studying chromatin biology. Our new, simplified, high-throughput ChIP kit reduces variability and significantly improves signal-to-noise ratios, even for low cell numbers. These Magna ChIP® HiSens kits, combined with the PureEpi™ chromatin prep kit, are particularly suited to new or novice users.
Stem Cell NeuroscienceCancerSignaling Cell StructureEpigenetics & Gene Regulation
ANtIboDIES, ASSAyS, SMAll MolECUlES, INHIbItoRS, AND PRotEINSNewVOLUME 2 I 2013
products
Magna ChIP® HiSens kits yield better fold enrichment than competitor’s low-input ChIP kits. Sonicated chromatin prepared from 10,000 HeLa cells was chromatin immunoprecipitated using 1 µg of purified IgG (Rabbit IgG: Catalogue No. 12-370) or specific antibodies (H3K4Me3: Catalogue No. 17-614, Phospho-CREB: Catalogue No. 17-10131). Immunoprecipitation of antibody-associated DNA fragments was verified by qPCR using positive control primers (GAPDH promoter primers for H3K4me3 and c-Fos promoter primers for pCREB) and negative control primers (human β-globin promoter primers). Results reflect analysis of 2 µL out of 50 µL total DNA per qPCR reaction.
Sonication parameters affect ChIP results! Use the PureEpi™ approach to pinpoint your perfect protocol. Here, ChIPAb+™ Phospho-CREB (Ser133)(Catalogue No. 17-10131) was used for ChIP, using the Magna ChIP® HT96 Chromatin Immunoprecipitation Kit (Catalogue No. 17-10077). ChIP results were analyzed using qPCR and the ΔΔ Ct method to calculate fold enrichment versus an IgG control at the positive locus.
PureEpi™ Chromatin Preparation & Optimization Kit optimized Chromatin Preparation for ChIP Great ChIP data starts with quality chromatin. The PureEpi™ Chromatin Preparation and Optimization kit is the first kit specifically designed to produce high quality chromatin for ChIP experiments.
The PureEpi™ kit helps you to avoid chromatin preparation pitfalls and reproducibly generate highly ChIPable chromatin. Discover the PureEpi™ approach and address multiple chromatin isolation and fragmentation parameters that impact ChIP performance.
Magna ChIP® HiSens Advantages• Validated, quality-controlled reagents designed to generate high
quality chromatin• Easy-to-follow and detailed protocols complete with optimization
guidelines for reproducible chromatin preparation. Read more at: www.emdmillipore.com/PureEpi
Magna ChIP® HiSensSupplier D
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Reli(Ab)le.You need more than results. You need results you can reproduce and defend. Every time. Without fail.
Reliable results depend on reliable resources. When you work with reliable antibodies, you can work efficiently and effectively. We guarantee performance because we manufacture performance. We are committed to manufacturing antibodies that perform as specified and as anticipated. Our quality starts at inception and carries through manufacturing, production and distribution, into the lab and onto the bench. From start to finish, we build a platform for reliable performance that provides you with the ultimate confidence in your findings.
Put the most reliable antibodies to work for you.www.emdmillipore.com/Ab
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New Antibodies, Kits, & Proteins
VOLUME 2 I 2013
Description Host Species Reactivity Key Applications Catalogue No.
AntibodiesAnti-26S proteasome regulatory subunit RPN11
Rabbit Hu, Ms WB ABE390
Anti-acetyl-p53(Lys120) Rabbit Hu Dot Blot, IP ABE286
Anti-APOBEC3G Rabbit Hu WB, IHC ABE607
Anti-Arylhydrocarbon receptor Rabbit Hu WB, IC ABE451
Anti-ASXL1 Rabbit Hu, Rt, Chimp, Rhe Mac, Bov, Can
WB, IHC(P) ABE400
Anti-BCL-11A Rabbit Hu WB, IC ABE401
Anti-CTCFL Mouse Hu WB, IF MABN632
Anti-FUS Mouse Hu WB, IHC, IC MABE465
Anti-HEXIM1 Rabbit Hu WB, IF ABE582
Anti-Histone H3.1/H3.2 Rabbit Ms, Hu WB, ChIP-seq, ChIP ABE154
Anti-hRNF20 Antibody Rabbit Hu, Ms, Rt WB ABE473
Anti-HuC/HuD, clone 15A7.1 Mouse Hu, Rt, Ms WB, IHC(P) MABN153
Anti-Lin28, clone 13D4.1 Mouse Hu WB, IC MABD53
Anti-MeCP2, Isoform B Rabbit Hu WB ABE333
Anti-MGEA5 Rabbit Hu WB, IHC, IF ABC283
Anti-NFE2L1 Rabbit Ms WB ABE584
Anti-NF-E2-related factor 2 Rabbit Hu, Ms, Rt, Chimp, Rhe Mac
WB, IC ABE413
Anti-PHD finger protein 11 Rabbit Hu, Ms WB, IC ABE396
Anti-phospho Cbx7 (Thr118) Rabbit Ms WB 06-1414
Anti-Puromycin, clone 12D10 Mouse Hu, All WB, IHC, IC, IF, FC, MABE343
Anti-Puromycin, clone 17H1 Rat Hu, All WB MABE341
Anti-Puromycin, clone 4G11 Mouse Hu, All WB, IC, IF, FC, IP MABE342
Anti-RBBP4, clone 6G5.1 Mouse Hu WB MABE221
Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2), clone 3E10
Rat Ms, Hu WB, ChIP 04-1571-I
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12
Rat Ms, Hu WB, ChIP 04-1570-I
Anti-Seryl-tRNA ligase, cytoplasmic Rabbit Hu WB, IHC ABN280
Anti-SHMT1 Sheep Hu, Ms WB, ChIP ABE416
Anti-SIRT4, clone 4F9.1 Mouse Hu WB, IHC MABE223
Anti-TARDBP Rabbit Hu WB, IHC, IF ABN271
Anti-Tet2 Rabbit Hu, Rt, Ms WB, IHC(P), IC ABE364
Anti-WAC, C-terminus Rabbit Hu WB, IP, ChIP ABE471
Anti-WAC, N-terminus Rabbit Hu, Ms WB, IP, ChIP ABE472
Anti-Xrcc1 Rabbit Hu, Ms, Rt WB, IP ABE559
Anti-Zscan4 Rabbit Ms WB, IF, IP AB4340
Epigenetics & Nuclear Function
lEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim= Primate, Por= Porcine, Chimp= Chimpanzee, Rhe Mac= Rhesus Macaque, Ham= HamsterApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry (Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB= Western Blotting, ChIP= Chromatin IP, ELISA= Enzyme Immunoassay, DB=Dot Blot
EMD Millipore’s newly released Anti-Puromycin, clone 12D10 and 4G11 monoclonal antibodies (Catalogue Nos. MAbE343 and MAbE342) have been published as part of a powerful and sensitive live cell analysis of protein synthesis: “SUnSET, a nonradioactive method to monitor protein synthesis *”
the authors used these anti-puromycin 12D10 and 4G11 (cited as 2D11) clones to reveal that puromycin was incorporated during protein synthesis, both with internal proteins and with external, type 2 proteins on live cells. they demonstrated that puromycin immunodetection with EMD Millipore’s antibodies is an advantageous alternative to radioactive amino acid labeling, because they enable direct visualization of protein synthesis in individual cells by high-resolution microscopy and may allow the detection of potentially specialized translation areas at subcellular scale, which has not been efficiently achieved until now, even with fluorescent puromycin derivatives. Protein translation can be directly quantified by flow cytometry, even in a subset of cells within larger heterogeneous populations or in cells exposed to pharmacological agents. their further testing on dendritic and t cells demonstrated the potential of SUnSEt for high-throughput drug screening. this technique will have multiple applications in virology, development, host-pathogen interactions, systems/network elucidation and other research areas.
*Schmidt EK et al. Nat Methods. 2009; 6:275-277.
PUblICAtIoN HIGHlIGHt oN NEW ANtIboDIES:
For published studies using this new product, visit: www.emdmillipore.com
For publications on using these small molecules, visit: www.emd4biosciences.com
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VOLUME 2 I 2013
For published studies using this new product, visit: www.emdmillipore.com
For publications on using these small molecules, visit: www.emd4biosciences.com
New Antibodies, Kits, & Proteins
New Small Molecules & Inhibitors
Description Details Catalogue No.
Epigenetic Regulators Panel - Acetylation A panel containing 18 potent and selective small molecule regulators that is useful for the study of epigenetic acetylation process.
324893-1EA
Epigenetic Regulators Panel - Methylation A panel containing 12 potent and selective small molecule regulators that is useful for the study of epigenetic methylation process.
324894-1EA
Haspin Kinase Inhibitor, CHR-6494 A cell-permeable indazolylimidazopyridazinamine compound that acts as a potent and reversible inhibitor of histone kinase haspin activity (IC50 = 2 nM) with moderate selectivity over TrkA, GSK-3β, PIM1, Cdk1/B and Cdk2/A (% inhibition at 100 nM = 58, 48, 36, 34 and 33, respectively) among a panel of 27-kinases.
372040-10MG
Histone Methyltransferase EZH2 Inhibitor, DZNep
A cell-permeable compound that is shown (at 1 µM concentrations) to inhibit EZH2-mediated trimethylation of K27 on histone H3 and induces the expression of cell-cycle regulatory genes, p21 and p27, as well as the cell death regulator, FBXO32, in OCI-AML3 and HL-60 cells, whereby treatment with inhibitor increases p16 levels in the former, but not the latter of the two cultures.
252790-2MG
Sirt1 Inhibitor VII, Inauhzin A cell-permeable phenothiazine derivative that selectively inhibits SirT1 (IC50 ≤2.0 µM), but not SirT2, SirT3, or HCAC8 (IC50 >50 µM), activity and effectively elevates cellular p53 lysine acetylation (Effective conc. 2 µM), thereby protecting p53 lysine residues from MDM2-mediated ubiquitination without directly inhibiting MDM2 function per se or its activity toward deacetylated p53.
566332-10MG
Epigenetics & Nuclear Function (continued)
lEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim= Primate, Por= Porcine, Chimp= Chimpanzee, Rhe Mac= Rhesus Macaque, Ham= HamsterApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry (Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB= Western Blotting, ChIP= Chromatin IP, ELISA= Enzyme Immunoassay, DB=Dot Blot
Description Host Species Reactivity Key Applications Catalogue No.
Kits & AssaysEZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit
ChIP 17-10461
Magna ChIP® HiSens Chromatin Immunoprecipitation Kit
ChIP 17-10460
PureEpi™ Chromatin Preparation and Optimization Kit
ChIP 17-10082
SIRTainty™ Class III HDAC Assay Enzyme Assay 17-10090
the novel SIRtainty™ class III HDAC Assay (Catalogue No. 17-10090) has just been published in a landmark SIRt1 study, headed by David Sinclair’s lab at Harvard University: “Evidence for a common mechanism of SIRt1 regulation by allosteric activators*”
Given that the SIRt1 deacethylase is a promising drug target for age-related disease therapy, the researchers explored the mechanism of sirtuin-activating compounds (StACs) using the SIRtainty™ assay. they found common motifs found in SIRt1 substrates could mediate SIRt1 activation by StACs. the Glu230 residue of SIRt1 was particularly critical for activation. together, the data indicated that SIRt1 can be activated by StACs through an allosteric mechanism.
*Hubbard bP et al. Science Mar 8 2013; 339: 1216-1219.
PUblICAtIoN HIGHlIGHt oN NEW ANtIboDIES:
Haspin Kinase Inhibitor, CHR-6494
EMD Millipore has introduced CHR-6494, a new cell-permeable, potent, and reversible inhibitor of histone kinase Haspin (IC50 = 2 nM) that exhibits moderate selectivity over a variety of kinases, including trkA, GSK-3β, PIM1, Cdk1/b and Cdk2/A. In the recent publication: “Anti-tumor activity of a small-molecule inhibitor of the histone kinase Haspin”
Huertas and colleagues showed that CHR-6494 has a wide spectrum of antitumor properties and can cause cell cycle arrest at the G2/M phase, leading to apoptosis. In addition, they demonstrated that it can reduce phospho histone 3 (thr3) levels in cells and cause metaphase misalignment, spindle abnormalities and centrosome amplification.
*Huertas, D et al. oncogene 2012; 31: 1408-1418.
PUblICAtIoN HIGHlIGHt oN SMAll MolECUlES:
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Cancer VOLUME 2 I 2013
Description Host Species Reactivity Key Applications Catalogue No.
AntibodiesAnti-ANGPTL6 Rabbit Ms, Rt WB ABC84
Anti-Annexin A2 Rabbit Hu, Rt, Ms, Prim, Por, Bov WB, IHC(P) ABC152
Anti-ARC, clone 6F9.1 Mouse Hu WB, IHC(P) MABC140
Anti-BAFF receptor Rabbit Hu, Ms, Rt WB, IHC ABC337
Anti-Bmf Rabbit Hu WB, IHC ABC348
Anti-Caspase-10 Rabbit Hu WB ABC343
Anti-Caspase-5 Rabbit Hu WB, IC, IF ABS445
Anti-Casper/c-FLIP , clone 17B12.1 Mouse Rt, Hu WB, IHC MABC148
Anti-CIDE-A Rabbit Ms WB, IHC, IF ABC350
Anti-Cyp7a1, clone 15B9.1 Mouse Hu, Rt, Ms WB, IHC MABD42
Anti-Deoxycytidine Kinase, clone 9D4 Mouse Hu WB MABC188
Anti-Ephrin-B2 Mouse Hu, Ms, Rt WB, IHC MABC127
Anti-FLI1 Rabbit Hu WB ABC291
Anti-HSPH1 Rabbit Hu WB, IF ABC264
Anti-hyaluronan synthase 1, clone 12A2.3 Mouse Hu WB, IHC MABC158
Anti-IRGM Rabbit Hu WB, IHC(P), IF ABC338
Anti-LAPTM4B Rabbit Ms, Rt WB ABC290
Anti-MAP1 Rabbit Ms, Hu WB ABC334
Anti-MDC1 Rabbit Hu WB, IC, IP ABC155
Anti-MGEA5 Rabbit Hu WB, IHC, IF ABC283
Anti-MUC-1, EC domain Rabbit Hu WB, IHC(P) ABC159
Anti-NAMPT Rabbit Hu, Ms WB, IHC ABC285
Anti-PDCL Rabbit Hu WB, IHC ABC284
Anti-Smac/Diablo, clone 3A9.1 Mouse Hu WB, IHC MABC174
Anti-Sox5 Rabbit Hu, Ms, Bov, Chicken, Zebrafish
WB ABC115
Anti-STEAP2 Rabbit Hu WB, IHC(P), IF ABC341
New Antibodies, Kits, & Proteins
New Small Molecules & Inhibitors
Description Details Catalogue No.
Ack1 Inhibitor, AIM-100
A cell-permeable furopyrimidine that acts as a potent, ATP-binding site-targeting, Ack1-selective inhibitor (IC50 = 22 nM) and effectively prevents receptor-mediated Ack1 activation (by >90% in MEF & LAPC4 cultures; 16 h 800 nM). Effectively inhibits androgen-independent LNCaP prostate cancer growth both in vitro and in castrated mice in vivo.
104833-10MG
HIF-1 Inhibitor, NSC-134754
A cell-permeable emetan derivative that selectively reduces HIF-1 activity by 73% in a luciferase reporter assay, and blocks HIF-1α protein induction in response to DFX, hypoxia, and IGF-1 in U2OS-HRE-luc cells at 10 µM, without significantly inhibiting cell growth.
400086-5MG
Mirk/Dyrk1B Inhibitor, Compo
A cell-permeable dihydropyridopyrimidine-carboxamide compound that acts as a potent and reversible inhibitor of Mirk/Dyrk1B and Dyrk1A kinases (IC50 = 68 and 22 nM). Shown to induce activation of caspase-3 and sensitize tumor cells to the action of gemcitabine and cisplatin, possibly by de-stabilizing p27.
475857-10MG
PIM3 Kinase Inhibitor VII, M
A cell-permeable hydroxyphenyl-propylidene-benzohydrazide compound that acts as a potent, ATP-competitive (Ki = 0.3 µM), and highly isoform-selective PIM inhibitor (IC50 = 2.5, 2.5, and 0.047 µM against PIM1, PIM2, and PIM3, respectively; [ATP] = 10 µM). Shown to inhibit PIM3-dependent STAT3 Tyr705 phosphorylation in DU-145 prostate cancer and MiaPaCa2 pancreatic cancer cells.
526526-10MG
Sphingosine Kinase 1 Inhibitor, SKI-178
A cell-permeable pyrazolocarbohydrazide compound that acts as a potent, reversible, sphingosine competitive and ATP-non-competitive inhibitor of sphingosine kinase 1 (SphK1) activity with excellent selectivity over SphK2 (Ki = 1.33 and >> 25 µM, respectively). Shown to suppress sphingosine-1-phosphate (S1P) production in A549 cells and efficiently induce apoptosis in several tumor cells.
567732-10MG
STAT3 Inhibitor XVIII, BP-1-102
A cell-permeable, orally bioavailable amidosalicylic acid compound that selectively binds the three subpockets of Stat3 SH2 domain surface (KD = 504 nM) and blocks Stat3 phosphorylation, dimerization, and DNA-binding activity in a dose-dependent manner. Shown to reduce Stat3-dependent growth, survival, migration, and invasion of tumor cells in vitro.
573132-10MG
lEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim= Primate, Por= Porcine, Chimp= Chimpanzee, Rhe Mac= Rhesus Macaque, Ham= HamsterApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry (Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB= Western Blotting, ChIP= Chromatin IP, ELISA= Enzyme Immunoassay, DB=Dot Blot
For published studies using this new product, visit: www.emdmillipore.com
For publications on using these small molecules, visit: www.emd4biosciences.com
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For published studies using this new product, visit: www.emdmillipore.com
For publications on using these small molecules, visit: www.emd4biosciences.com
VOLUME 2 I 2013
lEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim= Primate, Por= Porcine, Chimp= Chimpanzee, Rhe Mac= Rhesus Macaque, Ham= HamsterApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry (Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB= Western Blotting, ChIP= Chromatin IP, ELISA= Enzyme Immunoassay, DB=Dot Blot
Cell Structure
Inflammation & Immunology
Metabolism
Description Host Species Reactivity Key Applications Catalogue No.
AntibodiesAnti-ADAP Rabbit Hu WB, IC ABT342
Anti-ARC, clone 6F9.2 Mouse Hu WB, IHC(P) MABC140
Anti-CO-029 Rabbit Hu WB, IHC(P) ABT156
Anti-DOCK3 Rabbit Hu WB, IHC, IC ABT291
Anti-Girdin Rabbit Hu, Mky, Rt WB, IHC(P), IF, IP ABT168
Anti-Integrin α8 Rabbit Hu IHC(P) ABT139
Anti-Kinesin-like protein KIF11, clone 14B12.2
Mouse Hu WB, IC MABT152
Anti-KLHL1 Rabbit Hu, Rt WB, IHC ABT341
Anti-LGN Rabbit Ms, Rt WB ABT174
Anti-Nesprin-2 Antibody Rabbit Hu, Rt IC, IHC(P) ABT182
Anti-Obscurin Rabbit Hu, Ms IHC(P), WB ABT160
Anti-Osterix (SP7), clone 6B12.1 Mouse Hu, Rt WB, IHC MABT179
Anti-p115 RhoGEF Rabbit Hu, Ms, Rt WB, IC ABS208
Anti-Pinch-1, clone 9D2.1 Mouse Hu, Ms WB, IHC MABT162
Anti-Protocadherin 19 Rabbit Hu, Rt WB, IHC(P) ABT188
Anti-RHOC Rabbit Hu WB, IHC ABT295
Anti-Spectrin β chain, erythrocyte Rabbit Hu, Ms, Rt, Rhe Mac, Horse, Can
WB, IHC(P) ABT185
Anti-Spectrin β chain, brain 2 Rabbit Hu, Rt, Ms, Can, Rhe Mac IC, IHC(P) ABT183
Anti-Tom20/Tomm20, clone 2F8.1 Mouse Hu, Ms WB, IC MABT166
Anti-TOMM40 Rabbit Hu WB, IHC ABT296
Anti-TPM2 Rabbit Ms, Hu WB ABT301
Anti-Transgelin/SM22a, clone 20F2.2 Mouse Hu, Ms, Rt WB, IC MABT167
Description Host Species Reactivity Key Applications Catalogue No.
AntibodiesAnti-BAFF receptor Rabbit Hu, Ms, Rt WB, IHC ABC337
Anti-IL-12 α Rabbit Hu WB, IHC(P) ABN213
Anti-IRGM Rabbit Hu WB, IHC(P), IF ABC338
Anti-MDA5 Rabbit Hu WB, IHC(P), IF ABF210
Anti-NAMPT Rabbit Hu, Ms WB, IHC ABC285
Anti-SARM Rabbit Hu WB, IC, IF ABF219
Anti-Tirap Rabbit Hu WB, IHC, IF ABF211
Anti-TIRP Rabbit Hu, Ms, Rt WB, IHC, IF ABF207
Anti-TRIM5 α Rabbit Hu WB, IHC, IF ABF206
Anti-UNC93B1 Rabbit Hu WB, IHC(P), IF ABF215
Description Host Species Reactivity Key Applications Catalogue No.
AntibodiesAnti-ANGPTL6 Rabbit Ms, Rt WB ABC84
Anti-Cyp7a1, clone 15B9.1 Mouse Hu, Rt, Ms WB, IHC MABD42
Anti-IDH1 Rabbit Hu WB, IHC ABN267
Anti-STEAP2 Rabbit Hu WB, IHC(P), IF ABC341
New Antibodies, Kits, & Proteins
New Antibodies, Kits, & Proteins
New Antibodies, Kits, & Proteins
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For published studies using this new product, visit: www.emdmillipore.com
For publications on using these small molecules, visit: www.emd4biosciences.com
Description Host Species Reactivity Key Applications Catalogue No.
AntibodiesAnti-ACVR1C Rabbit Hu WB ABN476
Anti-Amyloid β (Aβ) x-4, clone 12F4 Mouse Hu IHC, ELISA 05-831-I
Anti-Aquaporin-4 Rabbit Hu, Ms, Rt WB ABN411
Anti-ARC, clone 6F9.3 Mouse Hu WB, IHC(P) MABC140
Anti-ATP2B1 Rabbit Rt, Hu, Can, Horse, Ms WB ABN258
Anti-Axin-2, clone 5H9.1 Mouse Hu IP, IHC MABN259
Anti-BubR1 Rabbit Hu, Ms WB ABS158
Anti-CHRNA3 Rabbit Hu, Ms WB, IHC ABN281
Anti-CTCFL Mouse Hu WB, IF MABN632
Anti-DARC Rabbit Ms WB, IHC(P) ABN495
Anti-DBN1 Rabbit Hu, Ms, Rt, Bov, Horse WB, IHC(P) ABN207
Anti-EPM2A Rabbit Hu WB ABN474
Anti-GABA Rabbit Rt, All IHC(P), ELISA ABN131
Anti-GABA(A)R β 1 Protein, clone N96/55 Mouse Rt, Ms IHC, WB MABN498
Anti-GABA(B)R1 Protein, clone N93A/49 Mouse Rt, Ms WB, IHC MABN492
Anti-GARS Rabbit Hu WB, IHC, IF ABS379
Anti-GluR2 Rabbit Ms, Rt, Hu WB, IHC(P) AB1768-I
Anti-GPR3 Rabbit Hu, Ms WB, IHC, IF ABN473
Anti-GPRASP1 Rabbit Hu WB, IHC ABN272
Anti-HuC/HuD, clone 15A7.1 Mouse Hu, Rt, Ms WB, IHC(P) MABN153
Anti-IL-12 α Rabbit Hu WB, IHC(P) ABN213
Anti-KCNK13 Rabbit Rt WB ABN484
Anti-LFG Rabbit Ms WB, IHC ABN478
Anti-MANF Rabbit Hu, Rt WB, IHC(P), IF ABN491
Anti-MAO-A, clone 15E12.1 Mouse Hu, Ms WB MABN230
Anti-MOB kinase activator 3B Rabbit Hu, Ms, Rt, Bov WB, IHC(P) ABN204
Anti-Monocarboxylate Transporter 1 Chicken Rt WB, IHC(P) AB1286-I
Anti-NANOS2 Rabbit Hu, Rt WB, IHC, IF ABN466
Anti-Neuropeptide S Receptor Rabbit Hu, Rt IHC(P) ABN12
Anti-Neuropilin-1, EC domain Rabbit Hu WB, IHC, FC ABN184
Anti-NHERF-2, clone 32B6 Mouse Hu, Rt WB, IC, IP MABN385
Anti-NIPSNAP3B Rabbit Ms WB, IHC ABN355
Anti-NSG1 Rabbit Hu, Ms, Rt WB, IHC(P) ABN257
Anti-OTUD4 Rabbit Hu WB, IC, IF ABN477
Anti-pan-TUC Rabbit Hu, Ms, Rt WB, IHC(P) ABN108
Anti-PRPF3 Rabbit Hu, Ms, Bov, Rt WB ABN202
Anti-RTP801 Rabbit Hu, Ms WB, IHC ABN483
Anti-Seryl-tRNA ligase, cytoplasmic Rabbit Hu WB, IHC ABN280
Anti-Sigma IR, clone ICR-SIG1R-A Rat Hu, Ms WB, IP, IC, FC, IHC MABN390
Anti-Slitrk1, clone 18G11.1 Mouse Hu, Ms WB, IHC MABD92
Anti-Slitrk3 Rabbit Hu WB, IHC ABN356
Anti-Slitrk6 Rabbit Hu WB, IHC ABN350
Anti-SPATA7 Rabbit Ms WB ABN269
Anti-SYNPO Rabbit Hu, Rt WB, IHC ABN481
Anti-TARDBP Rabbit Hu WB, IHC, IF ABN271
Anti-Tau, PAD, clone TNT-1 Mouse Hu, Ms, Rt WB, IHC,DB, IF, ELISA
MABN471
Anti-TFEB Rabbit Hu WB ABN472
Anti-UCHL1, clone MCA-BH7 Mouse Bov, Hu, Rt WB, IF MABN48
Anti-Wnt-10b Rabbit Hu WB, IHC ABN475
Proteins & EnzymesCysteine Sulfenic Acid Probe (DCP-Bio1) NS1226-1MG
Cysteine Sulfenic Acid Probe (DCP-Bio1) NS1226-5MG
New Antibodies, Kits, & Proteins
Neuroscience VOLUME 2 I 2013
lEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim= Primate, Por= Porcine, Chimp= Chimpanzee, Rhe Mac= Rhesus Macaque, Ham= HamsterApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry (Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB= Western Blotting, ChIP= Chromatin IP, ELISA= Enzyme Immunoassay, DB=Dot Blot
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VOLUME 2 I 2013
lEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim= Primate, Por= Porcine, Chimp= Chimpanzee, Rhe Mac= Rhesus Macaque, Ham= HamsterApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry (Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB= Western Blotting, ChIP= Chromatin IP, ELISA= Enzyme Immunoassay, DB=Dot Blot
For published studies using this new product, visit: www.emdmillipore.com
For publications on using these small molecules, visit: www.emd4biosciences.com
Description Details Catalogue No.
Adenosine A3 Receptor Antagonist, MRS 1523
A pyridine derivative that acts as a highly selective antagonist of A3 receptor with excellent potency in both humans and rodents (Ki = 18.9 nM for human A3R, and 113 nM for rat A3R).
119152-5MG
D-Amino Acid Oxidase Inhibitor III, AS057278
A cell-permeable pyrazolocarboxylic acid that acts as a selective D-amino acid oxidase (DAO/DAAO/DAMOX/OXDA) inhibitor (IC50 = 910 nM against human DAO; [D-ser] = 10 mM) and effectively protects DAO overexpressing cultures from oxidative stress-induced cell death upon D-Ser (50 mM) exposure (ED50 ≤3.95 µM), while exhibiting little affinity toward NMDAR glycine binding site or inhibitory activity against D-aspartate oxidase (DDO/DSOX).
251406-50MG
InSolution™ eIF4E/eIF4G Interaction Inhibitor, 4EGI-1
A cell-permeable hydrazone compound that reversibly binds eukaryotic translation initiation factor 4E (eIF4E; KD = 25 µM) and disrupts eIF4E/eIF4G complex formation. Shown to be brain permeant and reduce neuroligin protein levels, and restore L-LTP threshold and rescue autistic-like behaviours in Eif4ebp2 knockout mice.
324519-5MG
PSD95 Inhibitor, Tat-N-dimer A cell-permeable, dimeric pentapeptide (IETDV) derived from GluN2B C-terminal and linked through a polyethylene glycol (PEG)-based linker that Inhibits the ternary protein complex of PSD-95 (postsynaptic density protein-95), nNOS, and NMDA by directly binding to the tandem PDZ1 and 2 domains of PSD-95 (Ki = 4.6 nM). This inhibition results in the uncoupling between NMDA receptor activity and nitric oxide production.
529542-2MG
Neuroscience (continued)
New Small Molecules & Inhibitors
Signaling
Description Host Species Reactivity Key Applications Catalogue No.
AntibodiesAnti-acetyl-Tip60 (Lys523) Rabbit Hu, Ms, Bov, Por WB 07-1841
Anti-ACVR1C Rabbit Hu WB ABN476
Anti-Caspase-5 Rabbit Hu WB, IC, IF ABS445
Anti-CTRP3 Rabbit Ms WB, IHC, IF ABS451
Anti-GARS Rabbit Hu WB, IHC, IF ABS379
Anti-Gli3, clone 5E1 Mouse Hu, Ms WB, IC, IHC MABS275
Anti-Goosecoid 2 Rabbit Hu, Rt WB, IHC(P) ABS231
Anti-GPR3 Rabbit Hu, Ms WB, IHC, IF ABN473
Anti-LRP6, clone 1C10 Mouse Hu, Rt, Ms WB, IP MABS341
Anti-MIXL1 Rabbit Hu WB, IC ABS232
Anti-NLRP1 Rabbit Hu WB, IHC(P) ABF22
Anti-NLRP12 Rabbit Rt, Hu, Bov WB, FC ABF24
Anti-p115 RhoGEF Rabbit Hu, Ms, Rt WB, IC ABS208
Anti-PDCL Rabbit Hu WB, IHC ABC284
Anti-phospho-P70S6K (Thr470) Rabbit Hu WB ABS431
Anti-phospho-SRPK2(Ser497) Rabbit Hu, Ms, Ham WB ABS192
Anti-Wnt-10b Rabbit Hu WB, IHC ABN475
Proteins & EnzymesUbcH6, active Enzyme Assay 23-036
New Antibodies, Kits, & Proteins
9
Signaling (continued)
Description Details Catalogue No.
7a, 25-dihydroxycholesterol A highly potent and specific oxysterol EBI2 (GPR183) agonist with a kd of 450 pM in a saturation binding analysis, and activates EBI2 (EC50=140 pM compared with 2.1nM for its enantiomer 7b,25-OHC) in a GTP-γ assay. Stimulates migration of LPS-activated spleen B cells and T cells in a dose-dependent manner.
128136-1MG
AhR Antagonist III, GNF351 A cell-permeable purine compound that acts as a high-affinity aryl hydrocarbon receptor (AhR) antagonist (IC50=62 nM in mouse liver cytosol expressing humanized AhR) and lacks any agonist activity even at higher doses.
182707-10MG
CXCR3 Agonist, PS372424 A peptidomimetic derivative containing tetrahydroisoquinoline-arginine motif that aligns with a Pro-Arg motif of CXCL10. Acts as a highly specific agonist of human CXCR3 (EC50=1.1 mM, competitive CXCL10 binding IC50=42 nM). Effectively stimulates T-cell migration (~ 50 nM) and blocks the migration of activated T cells towards CXCL11, CXCL12, and CCL5
239823-5MG
ERO1 Inhibitor II, EN460 A cell-permeable thiol reactive enone (EN) compound that selectively interacts with the active-site cysteine of reduced, active form of ERO1α and inhibits its activity (IC50=1.9 µM). Also prevents ERO1 re-oxidation both in vitro and in mouse embryonic fibroblasts. Activates the unfolded protein response and protects ER-stressed 293T cells.
328501-25MG
FtsZ Polymer Stabilizer, PC1 A benzamide ether derivative that exhibits potent bactericidal activity against Staphylococcus aureus and Bacillus subtilis (minimum inhibitory concentration = 0.5 to 1 mg/mL), but does not affect E. coli, yeast, or human hepatocytes. Shown to reduce GTPase activity and induce FtsZ (a prokaryotic homologue of tubulin) polymerization in S. aureus in a dose-dependent manner.
344580-5MG
InSolution™ CFTR Inhibitor II, GlyH-101 A cell-permeable glycinyl hydrazone compound that acts as a potent, selective and reversible open-channel blocker of CFTR with intermediate speed (<1 min; 95% inhibition at 50 µM; Ki = 4.3 µM in CFTR-expressing FRT cells for apical membrane Cl- current) and exhibits desirable aqueous solubility.
219675-5MG
InSolution™ Fluvastatin, Sodium Salt A synthetic, competitive inhibitor of HMG-CoA reductase that acts as anti-hypercholesterolemic agent. Reduces the basal MMP-1 levels in culture media of endothelial cells in a time- and dose-dependent manner.
344096-10MG
InSolution™ HIF-1 Inhibitor A cell-permeable amidophenolic compound that inhibits hypoxia-induced HIF-1 transcription activity (IC50=0.7 and 2.6 µM using AGS and Hep3B cells, respectively, in a reporter assay) by selectively blocking the hypoxia-induced accumulation of cellular HIF-1α protein, while exhibiting no apparent effect on the cellular level of HIF-1α mRNA or that of HIF-1β protein.
400092-5MG
InSolution™ Lovastatin, Sodium Salt Carboxylate form of Lovastatin (Catalogue No. 438185) that is active in whole cells and cell-free assays. Lovastatin is reported to be an anti-hypercholesterolemic agent that acts as a reversible competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase.
438187-5MG
ITK Inhibitor, BMS-509744 A cell-permeable aminothioaryl-thiazolo compound that potently inhibits ITK kinase activity (IC50=19 nM; [ATP] = 1 µM) in an ATP-competitive manner by stabilizing ITK activation loop in a substrate-blocking, inactive conformation, inhibiting Fyn, IR, Lck, Btk only at much higher concentrations (IC50 ≥1.1 µM) and exhibiting little or no activity against 14 other kinases (IC50 ≥11 µM).
419820-5MG
Ketohexokinase Inhibitor An orally bioavailable pyrimidinopyrimidine compound that acts as a potent, reversible and ATP-competitive inhibitor of human hepatic KHK activity (KHK-C isoform; IC50 = 12 nM in a transcreener ADP assay) and efficiently blocks fructose-1-phosphate secretion (IC50 ≤ 400 nM in HepG2 cells).
420640-10MG
PDE10A Inhibitor, Cpd15 A cell-permeable pyrazoloquinoline compound that acts as a potent phosphodiesterase 10A inhibitor (Ki≤5 nM against human PDE10A), while exhibiting >500-fold selectivity over other human PDEs 1-11 and little activity against CYP3A4 (IC50 >20 µM) or a panel of 23 kinases (IC50 >30 µM).
513050-5MG
New Small Molecules & Inhibitors
lEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim= Primate, Por= Porcine, Chimp= Chimpanzee, Rhe Mac= Rhesus Macaque, Ham= HamsterApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry (Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB= Western Blotting, ChIP= Chromatin IP, ELISA= Enzyme Immunoassay, DB=Dot Blot
For published studies using this new product, visit: www.emdmillipore.com
For publications on using these small molecules, visit: www.emd4biosciences.com
10
VOLUME 2 I 2013 Signaling (continued)
Description Details Catalogue No.
Proprotein Convertase Inhibitor A cell-permeable tripeptide derivative containing an Arg-X-Arg motive that acts as a highly potent and reversible furin inhibitor (Ki=16 pM). Also shown to inhibit several other pro-protein convertases, such as PC1/3, PC4, PAEC4, PC5/6 with almost similar potency.
537076-2MG
PTP Inhibitor XXXI, II-B08 A cell-permeable indolo-salicylic acid compound that is shown to target SHP2 catalytic domain and inhibit SHP2-catalyzed pNPP hydrolysis (IC50=5.5 µM) in a reversible and non-competitive manner (Ki=5.2 µM). Effectively blocks hyperproliferative activity of human hematopoietic progenitor cells, as well as c-kit-dependent, SHP2-mediated myeloid proliferation.
565852-10MG
TLR4 Inhibitor, TAK-242 A cell-permeable cyclohexenecarboxylate that disrupts TLR4, but not TLR1-3 or TLR5-10, interaction with adaptor molecules TIRAP and TRAM via direct binding to TLR4 intracellular Cys747 residue.Shown to effectively inhibit TLR4-mediated cellular events (IC50=1.8, 1.3, and 1.9 nM, respectively, against LPS-induced NO, IL-6, and TNF-α production in murine RAW264.7 macrophages) in vitro.
614316-5MG
New Small Molecules & Inhibitors
tlR4 Inhibitor, tAK-242
EMD Millipore has recently introduced TAK-242, a new cell-permeable, fast acting, non-toxic compound that selectively blocks TLR4 signaling via binding to its intracellular domain and inhibits the production of multiple cytokines. TAK-242 selectively modifies Cys747 of TLR4 TIR (Toll/IL-1 receptor) and inhibits the interaction of TIRAP and TRAM with TLR4 in a dose-dependent manner. In the paper: “tAK-242 (resatorvid), a small-molecule inhibitor of toll-like receptor (tlR) 4 signaling, binds selectively to tlR4 and interferes with interactions between tlR4 and its adaptor molecules*”
Matsunaga et al from Takeda Pharmaceuticals demonstrated that TAK-242 binds selectively to TLR4 and subsequently disrupts the interaction of TLR4 with adaptor molecules, thereby inhibiting TLR4 signal transduction and its downstream signaling events. They also show that it inhibits the TIRAP-mediated activation of NF-κB.
*Matsunaga, N., et al. Mol. Pharmacology 2011; 79: 34-41.
PUblICAtIoN HIGHlIGHt oN SMAll MolECUlES:
Secondary & Control Antibodies
Stem Cell Research
Description Host Species Reactivity Key Applications Catalogue No.
AntibodiesAnti-HSV•Tag®,cloneHSV78 Mouse All WB MAC123
Sheep anti-Rabbit IgG, HRP conjugate Sheep Rabbit WB AP510P
Description Host Species Reactivity Key Applications Catalogue No.
AntibodiesAnti-CD73, clone AA60-E3-3 Mouse Hu IC, FC, WB, IP MABD122
Anti-Goosecoid 3 Rabbit Hu, Rt WB, IHC(P) ABS231
Anti-MIXL1 Rabbit Hu WB, IC ABS232
Anti-Sox5 Rabbit Hu, Ms, Bov, Chicken, Zebrafish
WB ABC115
Proteins & EnzymesFlowCellect® Human iPS Cell Characterization Kit
Human Hu FC FCSC100107
New Antibodies, Kits, & Proteins
New Antibodies, Kits, & Proteins
lEGEND Species: Hu=Human, Ms=Mouse, Rt=Rat, Bov=Bovine, Mky=Monkey, Can=Canine, Prim= Primate, Por= Porcine, Chimp= Chimpanzee, Rhe Mac= Rhesus Macaque, Ham= HamsterApplications: FC=Flow Cytometry, IC=Immunocytochemistry, IHC=Immunohistochemistry, IHC(P)=Immunohistochemistry (Paraffin), IF=Immunofluorescence, IP=Immunoprecipitation, WB= Western Blotting, ChIP= Chromatin IP, ELISA= Enzyme Immunoassay, DB=Dot Blot
For published studies using this new product, visit: www.emdmillipore.com
For publications on using these small molecules, visit: www.emd4biosciences.com
11
SmartFlare™ Probes - RNA Detection
Description Catalogue No.
SmartFlare™ miR-155-5p Hu-Cy5 SF-184
SmartFlare™ miR-16-5p Hu-Cy5 SF-178
SmartFlare™ miR-451a Hu-Cy5 SF-191
SmartFlare™ miR-17-5p Hu-Cy5 SF-180
SmartFlare™ miR-15b-5p Hu-Cy5 SF-486
SmartFlare™ miR-145-5p Hu-Cy5 SF-481
SmartFlare™ miR-29b-3p Hu-Cy5 SF-487
SmartFlare™ miR-31-5p Hu-Cy5 SF-445
SmartFlare™ miR-126-3p Hu-Cy5 SF-489
SmartFlare™ miR-143-3p Hu-Cy5 SF-488
SmartFlare™ miR-181a-5p Hu-Cy5 SF-451
SmartFlare™ miR-200c-3p Hu-Cy5 SF-450
SmartFlare™ miR-21-5p Hu-Cy3 SF-471
SmartFlare™ miR-30c-5p Hu-Cy5 SF-457
SmartFlare™ miR-132-3p Hu-Cy5 SF-452
SmartFlare™ miR-143-3p Hu-Cy3 SF-426
SmartFlare™ miR-15b-5p Hu-Cy3 SF-427
SmartFlare™ miR-29b-3p Hu-Cy3 SF-425
SmartFlare™ miR-29a-3p Hu-Cy5 SF-424
SmartFlare™ miR-125b-5p Hu-Cy5 SF-467
SmartFlare™ miR-223-3p Hu-Cy5 SF-495
SmartFlare™ miR-196a-5p Hu-Cy5 SF-468
SmartFlare™ miR-22-3p Hu-Cy5 SF-466
SmartFlare™ miR-150-5p Hu-Cy5 SF-435
SmartFlare™ miR-183-5p Hu-Cy5 SF-437
SmartFlare™ miR-194-5p Hu-Cy5 SF-434
SmartFlare™ miR-205-5p Hu-Cy5 SF-419
SmartFlare™ miR-215 Hu-Cy5 SF-429
SmartFlare™ miR-222-3p Hu-Cy5 SF-432
SmartFlare™ miR-542-3p Hu-Cy5 SF-420
SmartFlare™ miR-106b-5p Hu-Cy5 SF-436
SmartFlare™ miR-15a-5p Hu-Cy5 SF-430
SmartFlare™ miR-208a Hu-Cy5 SF-431
SmartFlare™ miR-101-3p Hu-Cy5 SF-403
SmartFlare™ miR-424-5p Hu-Cy5 SF-408
SmartFlare™ miR-30c-5p Hu-Cy3 SF-441
SmartFlare™ miR-199b-3p Hu-Cy5 SF-443
SmartFlare™ miR-18a-5p Hu-Cy5 SF-496
SmartFlare™ miR-1 Hu-Cy5 SF-478
SmartFlare™ miR-182-5p Hu-Cy5 SF-480
SmartFlare™ miR-33a-5p Hu-Cy5 SF-155
SmartFlare™ miR-184 Hu-Cy5 SF-156
SmartFlare™ miR-15a-5p Hu-Cy3 SF-159
SmartFlare™ miR-122-5p Hu-Cy5 SF-160
SmartFlare™ miR-196a-5p Hu-Cy3 SF-161
Description Catalogue No.
SmartFlare™ miR-29a-3p Hu-Cy3 SF-177
SmartFlare™ miR-205-5p Hu-Cy3 SF-163
SmartFlare™ miR-199b-3p Hu-Cy3 SF-169
SmartFlare™ miR-424-5p Hu-Cy3 SF-170
SmartFlare™ miR-18a-5p Hu-Cy3 SF-172
SmartFlare™ miR-22-3p Hu-Cy3 SF-701
SmartFlare™ miR-146b-5p Hu-Cy5 SF-479
SmartFlare™ miR-190a Hu-Cy5 SF-409
SmartFlare™ miR-9-3p Hu-Cy5 SF-715
SmartFlare™ miR-140-3p Hu-Cy5 SF-716
SmartFlare™ miR-182-5p Ms-Cy5 SF-717
SmartFlare™ miR-181a-5p Hu-Cy3 SF-718
SmartFlare™ miR-222-3p Hu-Cy3 SF-719
SmartFlare™ miR-221-3p Hu-Cy5 SF-470
SmartFlare™ miR-9-5p Hu-Cy5 SF-497
SmartFlare™ miR-24-3p Hu-Cy5 SF-726
SmartFlare™ miR-150-5p Hu-Cy3 SF-727
SmartFlare™ miR-130a-3p Hu-Cy5 SF-809
SmartFlare™ miR-137 Hu-Cy5 SF-813
SmartFlare™ miR-224-5p Hu-Cy5 SF-835
SmartFlare™ miR-23a-3p Hu-Cy5 SF-837
SmartFlare™ miR-328 Hu-Cy5 SF-849
SmartFlare™ miR-486-5p Hu-Cy5 SF-863
SmartFlare™ miR-708-5p Hu-Cy5 SF-871
SmartFlare™ miR-183-5p Hu-Cy3 SF-890
SmartFlare™ miR-140-3p Hu-Cy3 SF-893
SmartFlare™ miR-146a-5p Hu-Cy5 SF-500
SmartFlare™ miR-134 Hu-Cy5 SF-811
SmartFlare™ miR-154-5p Hu-Cy5 SF-819
SmartFlare™ miR-186-5p Hu-Cy5 SF-823
SmartFlare™ miR-188-5p Hu-Cy5 SF-825
SmartFlare™ miR-28-5p Hu-Cy5 SF-839
SmartFlare™ miR-28-3p Hu-Cy5 SF-841
SmartFlare™ miR-302a-3p Hu-Cy5 SF-847
SmartFlare™ miR-335-5p Hu-Cy5 SF-851
SmartFlare™ miR-338-3p Hu-Cy5 SF-853
SmartFlare™ miR-449a Hu-Cy5 SF-861
SmartFlare™ miR-491-5p Hu-Cy5 SF-865
SmartFlare™ miR-320b Hu-Cy5 SF-899
SmartFlare™ miR-145-5p Hu-Cy3 SF-903
SmartFlare™ miR-9-3p Hu-Cy3 SF-907
SmartFlare™ miR-146b-5p Hu-Cy3 SF-909
SmartFlare™ miR-190a Hu-Cy3 SF-1072
SmartFlare™ miR-130a-3p Hu-Cy3 SF-810
SmartFlare™ miR-708-5p Hu-Cy3 SF-872
Introducing SmartFlare™ RNA Detection Probes! Detect miRNA in LIVE Cells!Now you can effortlessly detect and quantify
RNA in LIVE cells using the platform of your
choice. Furthermore, you can continue using
those same, unperturbed cells for downstream
analyses! All this can be done with:
• No sample prep
• No nucleic acid amplification
• No transfection
• No cell lysis
• No toxicity
miRNA Detection using SmartFlare™ RNA Detection ProbesmiRNA detection has been plagued with dif-
ficultly due to the short nature of the biomol-
ecules, limiting designs of detection probes.
Attempts to quantify expression have included
qPCR methods using hairpin probe designs,
which are fraught with amplification errors and
nonspecific binding. SmartFlare™ RNA Detection
Probes are superior, because they can detect
short sequences with high specificity. See our
SmartFlare™ probes specific to miRNA targets
listed at right.
For a complete list of probes and
more information including application
notes and demo videos, visit:
www.millipore.com/smartflare
VOLUME 2 I 2013
EMD Millipore, the M logo, PureEpi, and SmartFlare are trademarks of Merck KGaA, Darmstadt, Germany. FlowCellect, Magna ChIP, Novatope, Anti-HSV Tag, Chemicon, Upstate, and Calbiochem are registered trademarks of Merck KGaA, Darmstadt, Germany.All trademarks belonging to third parties are the properties of their respective owners. Lit No. PR5545EN00 BS-GEN-13-08314 Printed in the USA. 04/2013 © 2013 EMD Millipore Corporation, Billerica, MA USA. All rights reserved.
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