new insight into hbv evolution - genaforevolution: mummy hbv near-identical to contemporary strains....
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Jan Felix Drexler
Institute of Virology, University of Bonn
New insight into HBV evolution
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How old are hepadnaviruses – endogenous bird & reptile
variants well >60 million years
Suh, PLoS Gen, 2015
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Consistent with diverse hepadnaviruses in lower
vertebrates
Dill, J Virol, 2016
Mammal-like?
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Hepatology, 2012
Slow long-term evolution:
Mummy HBV
near-identical to contemporary
strains
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Extant bats are old mammalian lineages – introduction of
ancestral HBV into mammals?
Simmons, Science 2005
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Screening of 3,000 bats for HBV
Drexler/Geipel et al., 2013, PNAS
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Roundleaf bat H. Ruber RBHBV
Tent-making bat U. Bilobatum TBHBV
Horseshoe bat R. Alcyone HBHBV
natalie's Life List, inaturalist.org
3 viruses
1
2 3
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Relevance of bats - Chiropteran hosts harbor
largest hepadnavirus diversity within mammals
Rasche et al., Curr Opinion Virol, 2016
Two HBV species in primates: HBV WMHBV
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Primate HBV ancestors: Acquisition of bat viruses by
monkeys?
Rasche et al., Curr Opinion Virol, 2016
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Lack of studies into New World non-human
primates
123 animals 9 species
4 studies in 45 years
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Brazil: 5th largest country Highest primate diversity
worldwide
Brazil 118 species
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Screening of ca. 100 NHP A new primate HBV species:
CMHBV
Dominguez/König/Rasche et al., unpublished
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Potentially chronic infection: core deletions and no signs
of inflammation
Dominguez/König/Rasche et al., unpublished
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Deep HBV sister lineages in new world monkeys
Dominguez/König/Rasche et al., unpublished
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Evidence for ancient co-speciation of primate
hepadnaviruses and their hosts – but not for HBV
Dominguez/König/Rasche et al., unpublished
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Ancestral state reconstructions
Primate hepadnavirus origins in NHP HBV in humans?
Dominguez/König/Rasche et al., unpublished
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Hypothesis testing
HBV may have evolved in hominoid ancestors 17 million years ago
Dominguez/König/Rasche et al., unpublished
You are here!
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No evidence for ancestry of F/H
in American monkeys
Introduction
along human migration?
Dominguez/König/Rasche et al., unpublished
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In sum • Hepadnaviruses are an extremely old
virus family • Primate hepadnaviruses may be the
result of a host switch millions of years ago
• HBV likely evolved in hominoid ancestors, preceding the human stem lineage
• Divergent New World genotypes F/H may originate from pre-historic human migration
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Virology, Bonn Christian Drosten Victor Max Corman Monika Eschbach-Bludau Sebastian Brünink Tobias Bleicker Andrea Rasche Marcel Müller FLI, Riems Rainer Ulrich Martin Beer
Ulm Elisabeth Kalko Marco Tschapka Stellenbosch Wolfgang Preiser Sonja Matthee Kruger NP Danny Govender
EMC, Rotterdam Ab Osterhaus Thijs Kuiken Debby van Riel JMA van den Brand Virology, Berlin Detlef Krüger Virology, Giessen Dieter Glebe Virology, Heidelberg, Ralf Bartenschlager Virology, Hannover Georg Herrler Bernhard Nocht Institut, HH Thomas Kruppa Jonas Schmidt-Chanasit RIVM, Netherlands Chantal Reusken ILRI, Kenya Jörg Jores
Funded by
KCCR, Kumasi Augustina Annan Samuel Oppong Mexico Álvaro Aguilar Setién Veterinary Medicine, Salvador Roberto Franke Eduardo Netto Chumakov Institute, Moscow Alexander Lukashev CIRMF, Franceville Eric Leroy AFMB, Marseille Bruno Coutard