neurobiology i axon guidance - iiser puneraghav/pdfs/neurobiology1/... · robol rob03 slit netrin...
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Neurobiology IAxon Guidance
20130916
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Why guidance is important?
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Lowery and Van Vactor, 2009
Road map and signposts
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Axon GuidanceAbility of the growing axon to navigate its way through the complex landscape of the developing embryo to its correct target tissue and establish synaptic communication with it.
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Pioneer axon navigation
Bentley and Caudy, 1983
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• Growing axons are able to recognize various molecules on the surfaces of other
axons and cells, and to use these molecules as cues tonavigate the sometimes circuitous pathways to theirparticular destinations.
• Also respond to diffusible molecules such as morphogens.
• They respond to same cues differently during different developmental stages, thus giving different outputs.
Hibbard, 1965
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Chemoattraction at the CNS midline
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• Attraction to midline: due to activation of DCC by netrins.
• Crossing and moving from the midline:
1. Upregulation of Robo expression and repulsion by Slit
2. Loss of netrin responsiveness even if DCC is expressed.
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Cytoskeletal remodeling in guidance
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Mechanical tension and axonal outgrowth
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Thank You!
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Dickson and Gilestro, 2006.