neufeld citizen science
DESCRIPTION
Presentation given to Citizen Science workshop in January 2012.TRANSCRIPT
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Aquarium microbial ecology: a living room approach to citizen science
Josh D. Neufeld University of Waterloo
@joshdneufeld
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AOA in Natural Environments
marine estuary
freshwater soil
AO
A a
re u
biq
uito
us A
OA
outnumb
er AO
B
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Ammonia oxidation in built aquatic environments?
Access to filters… scistarter
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Sample from a variety of aquaria: freshwater, marine, fish, plants
Collect from KWAS members, extract DNA, quantitative real-time PCR for ammonia-oxidizing archaea (AOA) and ammonia-
oxidizing bacteria (AOB).
Fingerprinting and sequencing for diversity.
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UNANSWERED QUESTIONS What aspects of water chemistry shape aquarium AOA (and AOB) communities? Do AOA/AOB communities correlate? Does AOB/AOA biofilm succession occur when aquariums are first established? From where do filter AOA seed populations originate? Do they seed homes (“x”)? Do AOA buffer aquaria from ammonia toxicity during antibiotic treatment?
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ADVANTAGES OF AQUARIUMS FOR CITIZEN SCIENCE Aquarium hobbyists are organized by societies, pet stores – already networked Young people are involved and invested (children, schools, undergrads) Individual results matter to aquarists Aquaria are aquatic microcosms, “experiments” – already citizen science! Standalone or accessory to existing “Domestic Biome” initiatives High biomass, easy sampling
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AQUARI-US PROJECT
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University of Waterloo *Laura Sauder(MSc)*
Jennifer Stearns (PDF) Katja Engel (RA)
Andre Masella (RA) Richard Pawliszyn (BSc)
Puntipar Sonthiphand (PhD)
NIOZ Roy. Netherlands Institute for Sea Research
Francien Peterse Stefan Schouten
Others
Kitchener Waterloo Aquarium Society
KW Aquarium stores
Guelph Wastewater Treatment Plant
Region of Waterloo
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[email protected] thank you @joshdneufeld