name sense antisense gtctccatcagtggctctacgcccggttcttgttgtattttgvegfr1...
TRANSCRIPT
Name sense antisense
GTCTCCATCAGTGGCTCTACG CCCGGTTCTTGTTGTATTTTGVEGFR1
CTGCCTACCTCACCTGTTTCC CGGCTCTTTCGCTTACTGTTCVEGFR2
GGAAGGCTCTGAAGATAAAGG ACAGAAGATGAGCAGGAGGAGVEGFR3
CTCTGAAGAAGTGGGGTCTGG AAGTAGATGGTGGGCAGGAAGCXCR4
GAPDH ACATCAAGAAGGTGGTGAAGC AAGGTGGAAGAGTGGGAGTTG
Supplemental Table 1:
PSGL-1 GAGAAGATTGCCACCACTGAC GTTGGACGGTCTCTACTGAGG
h-PSGL-1 CAATTTGTCCGTCAACTACCC TGCACACGAAGAAGATAGTGG
h-GAPDH GAAGGTGAAGGACGGACTC GAAGATGGTGATGGGATTTC
Real - time PCR primers used in the present study
h-P-selectin GAAGGTGAAGGACGGACTC GAAGATGGTGATGGGATTTC
GGCTGCTGGCTTCTAAGTGTG TTCCGTGACCGGTAAGTATTGTP
E-selectin TGACTTGCAAACCACAGGAAC TTACAGCCAAAGCAGCAGGAG
<CVR-2014-1358>
WT50
100
150 *,**P<0.05
***
Ble
edi
ng t
ime
N=6
0.5
1.0
1.5
2.0
Pla
tele
t co
unts
0
2.5
5
7.5
10
Meg
aｋ
aryo
cyte
s /
field
WT
A.N=6
IP-/-WT TP-/-
B.
WTC.
D. N=6
IP-/-TP-/-
IP-/-TP-/-
IP-/-TP-/-
Supplemental Figure I
<CVR-2014-1358>
0
0.2
0.4
0.6
0.8
1
1.2
0
20
40
60V
EG
F(p
g/m
l)
IgG P-selectin antibody
IgG P-selectin antibody
WT TP-/-
NS NS
IgG P-selectin antibody
IgG P-selectin antibody
WT TP-/-
NS NS
SD
F-1
(ng
/ml)
Not ligated
A.
B.
Supplemental Figure II
<CVR-2014-1358>
0 24 48 72
P
lasm
a le
vel o
f p
ro M
MP
-9(n
g/m
l)
TP-/-WT
* *
0
30
60
Time (hours)
A.
100
150
200
250
300
TP-/-
WT
0 24 48 72
Pla
sma
leve
l of
SC
F (
pg/m
ｌ)
* **
Time (hours)
B.
Supplemental Figure III
<CVR-2014-1358>
radiation
0
50
100
150
200
250
0
2000
4000
6000
8000
10000
0
50
100
150radiation
0
5000
10000
15000
WT
*
0 7 14 21 28
WT
*
*
0 7 14 21 28(days) (days)
Num
ber
of
Pla
tele
ts (
104/
l)
Num
ber
of
WB
C (
104/
l)
WT
*
Pla
sma
leve
l of
SC
F (
pg
/mｌ
)
WT
BM
le
vel o
f p
ro-M
MP
9 (
ng
/mｌ
)
*
TP-/- TP-/-
TP-/- TP-/-
A. B.
C. D.
Supplemental Figure IV
<CVR-2014-1358>