naktuinbouw laboratories price list 2020...laboratories page 4 / nematodes tarievenlijst_eng juni...

Naktuinbouw Laboratories

Price list 2020

Laboratories Page 1 Tarievenlijst_ENG juni 2020

* Assignments for plant material: Samples of the same crop are jointly registered in one assignment. The basic fee will be charged once.

* Assignments for seeds and soil: Each sample is registered as a separate assignment. The basic fee is charged per sample.

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*Submission forms for the different types of orders can be found at our website, they can also be requested at the administration

([email protected])

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* If you have more questions about sampling, please contact our account manager ([email protected])

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Information about the price list and instructions

Special conditions apply to the submission of material which has to be made free from viruses. You can obtain more information at Naktuinbouw Test Center in Horst (Netherlands);

[email protected] or by phone on +31 77 398 59 19.

If needed you can send pictures to [email protected]

Send a whole plant if possible. Leaf symptoms may be caused by root problems.

Pack roots and leaves in such a way that leaves cannot be polluted by soil, e.g. by wrapping the roots in a separate plastic bag and sealing it with a elastic band around the stems.

Requests for seed analysis, seed health testing and nematode testing, can be submitted electronically through myNaktuinbouw. You enter the data of the sample and select the appropriate

tests. Subsequently, the sample can be sent to Naktuinbouw. When results of the tests are ready, they are available at myNaktuinbouw. You will receive the certificates as you're used to.

More information can be found on www.naktuinbouw.com or feel free to contact us via: [email protected].

If you state on your shipment that it is URGENT and has to be given priority, the sample will be prepared and tested as soon as possible. An extra urgency rate will apply.

myNaktuinbouw

General instructions on submitting samples

Basic fee 2020

Liquid samples must be submitted in vapour-tight packaging.

The material must be packaged in plastic and the packaging must be sealed properly. Send the sample in a sturdy box. Secure the submission form in the parcel.

A basic fee will be charged per assignment. The basic fee is € 25,- and € 0,35 per sample. For assignments via mijnNaktuinbouw, the basic fee is € 15,- and the sample fee is € 0,35 per

sample.

For shipments from outside the European Union we have a sticker available to send samples without a phytosanitary certificate. For inquiries please contact our lab administration.

([email protected]).

Submitting seeds

Seed tests have been developed and validated for untreated seeds. Since the reliability of health tests may be affected if seeds have been treated, submitted seed samples should preferably

be untreated. This does not apply for tests where it is stated that it can be applied on treated seeds.

If seeds have been disinfected, coated or pilled, please inform us accordingly for the sake of the safety of our employees and possible influence on test results.

Submitting material to make it free from viruses

Submitting material for diagnostic testing

Submitting plant material

Additional instructions

Make sure your plantmaterial is at our laboratory before or on the Wednesday prior to the test week as we are closed in the weekends; material received on Friday can't be proceeded.

Laboratories Page 2 / Information & Instructions Tarievenlijst_ENG juni 2020

Contacting Naktuinbouw Laboratories

You can contact the Laboratory Administration directly for any questions and assignments.

Administration +31 71 332 61 50

Planning +31 71 332 62 40

e-mail [email protected]

Shipping address for all samples Delivery address for courier services

Naktuinbouw Naktuinbouw

attn. Administration Laboratories attn. Administration Laboratories

P.O. Box 40 Sotaweg 22

2370 AA Roelofarendsveen 2371 GD Roelofarendsveen

The Netherlands The Netherlands

Tel. +31 77 398 59 19

e-mail [email protected]

Terms and conditions of delivery and disclaimer

If testing / diagnosis is not carried out by Naktuinbouw but, after having consulted the client , is subcontracted to a third party, the corresponding costs will be charged to the client.

If you state on your package that it concerns an URGENT assignment, an urgency rate will be apply.

Diagnostic samples are always priority; an extra urgency rate will apply.

Additional testing may be required if a possible pathogen is found. Extra costs for confirmation testing will be charged

The results of tests or assessments provided by Naktuinbouw Laboratories only relate to the actual sample tested and not to the lot concerned, unless Naktuinbouw states otherwise.

Contact with Naktuinbouw Laboratories

If you have any specific laboratory questions regarding diseases in fruit crops, arboricultural crops or strawberries, contact Test Center Horst:

For speciific diagnostic enquiries please call (071) 332 62 55 or email [email protected]

The General Terms and Conditions of Naktuinbouw apply to all services provided by the Naktuinbouw Laboratories (registered with the Chamber of Commerce and Industry for Rijnland in Leiden, the

Netherlands, under no. 41150707). You can find on our website: www.naktuinbouw.com/terms-and-conditions

All amounts stated are excluding VAT. Changes to rates are reserved.

Laboratories Page 3 / Contact Tarievenlijst_ENG juni 2020

mailto:[email protected]:[email protected]

Test rate

Standard tests on soil Base rate per sample

€ 24,70

€ 42,50

€ 99,00

€ 146,60

€ 42,50

Standard tests on plantmaterial / water Rate

€ 47,80

€ 60,70

€ 146,60

€ 146,60

Testing for Nematodes

Nematodes can seriously harm crops. Preventative testing of the soil, seeds and plant material can prevent this. Naktuinbouw offers testing for various plant-parasitic nematodes

which may harm your crops. For example, the presence of Xiphinema and Longidorus species in soil may be demonstrated as part of the Elite certification procedure for

strawberries.

Onion sets, or seed, can only be marketed if accompanied by a statement that the onion sets or the seed in question are free from stem nematode (Ditylenchus dipsaci). Other

crops also require a statement that they are free from nematode when exported to certain destinations. Naktuinbouw can help you with this. If you would like further information,

please contact the Laboratories Administration Team.

1500 ml

Standard test for all present plant parasitic nematodes 2

Standard test for one plant parasitic nematode 2

Xiphinema spp. / Longidorus spp.

200 ml

200 ml

10 grams

All rates are excluding sampling. The customar has to take care of sampling or you can ask Naktuinbouw to do this for you. You can contact Naktuinbouw Inspections

Department to take samples for certification purposes.

Sample size

Determination Radopholus spp. 3 substrate

Sample size

200 - 300 ml

min. 25 gram

200 ml

Determination Radopholus spp. 3 in root material

Determination Ditylenchus spp . in consumption onions 3

Standard test for all plant parasitic nematodes present 2

Standard test for one plant parasitic nematode 2

min. 25 gram

Only Ditylenchus

60 pce

Laboratories Page 4 / Nematodes Tarievenlijst_ENG juni 2020

Standard tests on seed Rate

€ 30,20

€ 30,20

Rate

€ 127,20

€ 72,80

€ 24,60

€ 20,20

2, Naktuinbouw Labaratories can determine the genus for the following nematodes.

3, Rates are inclusive priority treatments and basic rate

•

Paratylen

chus

spp.*

•

* Mentioned nematodes can be determined to species with an extra fee.

• Aphelenchoides spp.*

• Ditylenchus spp.*

• Helicotylenchus spp.*

• Hemicycliophora spp.

• Longidorus spp.

• Meloidogyne spp.*

In case sampling is carried out by Naktuinbouw, sampling costs will be charged by the Department of Inspections

Extra fee for determination on species level 3

Extra fee for incubation (This will take two additional weeks)

Surcharge urgency rate

Other

30.000 seeds (max 500g)

30.000 seeds (max 500g)

Rate per hour for extra research (if needed)

Aphelenchoides spp.2

Ditylenchus spp.2

Sample size

Laboratories Page 5 / Nematodes Tarievenlijst_ENG juni 2020

Rate

Standard diagnosis, first sample € 243,00

Each additional sample € 113,00

Limited diagnosis, first sample (usually visual or microscopic assessment) € 190,00


Testing for up to 5 viruses, first sample € 130,00


Testing for up to 10 viruses, first sample € 154,50

Each addittional sample € 82,40

PCR testing per sample 4 € 108,00

Rate per hour for additional work (if needed) € 141,40

Verification test (if applicable) € 37,60

BasistoetsIF/ microscope/

agglutination

Confirmation

PCR

Erwinia amylovora € 168,00 € 47,30 € 62,50

Phytophthora fragariae (visuele diagnose) € 150,00

Xanthomonas arboricola pv. pruni € 184,00 € 106,00

Xanthomonas fragariae € 139,00 € 106,00

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4 An extra PCR test is carried out when final identification of a pathogen is needed.

Diagnostics

Diagnostics brings the practice of horticulture, the diagnosticians and the researchers of Naktuinbouw Laboratories together. Annually about 250 different crops from numerous countries are studied. The wide experience makes

Diagnostics a good partner for reliable diagnoses of diseases, pests and cultivation problems. Please note that our Diagnostics team only performs tests on material with symptoms.

Our Diagnostics Team works with a fixed rate. The advantage for the person submitting samples is that the costs of diagnostic testing are known in advance. Assignments are immediately processed. And savings can also be made

on the handling and administrative costs, which will help keep the rates down. The rates below are including the urgency rate. Our Diagnostic Laboratory can handle virus tests for limited-scope diagnosis such as TSWV, INSV and

PepMV rather quickly and the applicant can have the test result usually within one day.If necessary, found organisms can be further identified with the help of molecular techniques We have seen that there is demand for having

samples tested for certain viruses fast. This often concerns TSWV, INSV,and PepMV. Naktuinbouw has the facilities to enable such limited-scope diagnoses to be carried out rapidly, so that the person submitting the sample will

often get the results within one day.

Composition of the test rate

Rates for diagnosis

The rate for any succeeding additional sample only applies to samples of the same assignment and same crop.

Extra costs for additional services provided, such as sampling or transport, will be charged invoiced.

If an assignment is clearly so extensive that additional costs must be incurred it cannot possibly be done for these rates, we will contact you in advance.

Laboratories Page 6 / Diagnostics Tarievenlijst_ENG juni 2020

Rates for ELISA tests

Group 1

Rate

€ 2,50

Group 2 Sample size Test code Rate

1 CORM PER TEST FREMVEKK € 5,25

5 A confirmation PCR of € 64,60 may follow

6 If you provide material for testing on poty virus, please send double the amount of material as this is required to process the specimen.

Freesia mosaic virus corms and cormels

A quick test is available for some about 70 viruses in floricultural and arboricultural crops: the ELISA. This technique can be used routinely and gives quick results. produces results fast. ELISA is a

robust technique with reliable results and a relatively low cost price compared to other techniques. Testing with indicator plants or PCR is also possible. These tests are only carried out on leaf

material. Tissue culture plants show a reduced sensitivity in the ELISA. The sensitivity of the ELISA test in tissue culture plants is less.

Health Testing of Floriculture and Arboricultural Crops

Virus test methodes

Virus

AlCV (LSV), AlMV, AltMV (PapMV), AMV, AnFBV (AFMoV), ArMV, AV1, BBWV, BWYV, BYMV, CarMV, CbMV, CERV, CLV, CMV,

CNFV, CRSV, CSNV, CVB, CVMV, CymMV 5, FreSV (FOV), FreMV, HdRSV, HVX, INSV, IYSV, KLV, KMV, ORSV

5, OYDV, PDV,

PEBV, PepMV 5, PFBV, PLPV, PMMoV, PnMV, PNRSV, Potyvirus

6, PVX, PVY, PZSV, RBDV, RMV (TVCV, YoMV), ScrMV

(NeRNV), SFBV, SLV, SYNV, TAV, TBRV, TBSV (PLCV, PetAMV), TMGMV, TMV-WU1, TNV1, TNV2, ToMV-DO3, ToRSV, TRSV,

TRV, TSV, TSWV 5

Fungal tests Testing for fungi is are usually done by a plating method, combined with identification by microscope. New DNA techniques are used for sensitive detection of e.g. Fusarium species in

begonia. Fungal tests Testing for fungi can also be done on soil samples, e.g. for Verticillium dahliae and Fusarium oxysporum spp.

Virus tests ,viroids and phytoplasm tests

Seed and plant material can be tested for the presence of viruses, viroids or phytoplasms, using the latest new molecular techniques.

Other virus tests

Fungal tests

Seed and plant material can be tested for the presence of bacterial diseases. Some of the methods used are: plating on semi selective media, pathogenicity tests, immunofluorescence (IF) and

PCR.

Bacterial

Laboratories Page 7 / Health in Floriculture crops Tarievenlijst_ENG juni 2020

Viruses, Viroids and Phytoplasma Sample size Test code Basic testIF/ microscope/

agglutinationConfirmationPCR

PSTVd, TCDVd, TPMVd real-time RT-PCR Max. 25 leaves PSTVD € 123,70

CChMVd real-time RT-PCR max. 25 leaves CCHMVDP € 68,80

CSVd real-time RT-PCR max. 25 leaves CSVDP € 68,80

CSVd, CChMVd real-time RT-PCR Max. 25 leaves COMBICSVDCCHMVD € 68,80

Pospiviroids (CEVd, CLVd, CSVd, IrVd1, MPVd, PCFVd, PSTVd,

TASVd, TCDVd, TPMVd) real-time RT-PCRMax. 25 leaves POSPIP € 123,70 € 108,20

Pospiviroids (CEVd, CLVd, CSVd, IrVd1, MPVd, PCFVd, PSTVd,

TASVd, TCDVd, TPMVd) real-time RT-PCR (screening)max. 200 leaves POSPIQSCREEN € 389,80 € 108,20

Dahlia common mosaic virus, Dahlia mosaic virus species complex

(DMV / DCMV) real-time RT-PCRMax. 25 leaves DMVQ € 68,80

Plum pox virus (PPV) real-time RT-PCR stem material PPVP € 108,20

Plum pox virus (PDV) real-time RT-PCR Max. 25 leaves PDVQ € 68,80

Prunus necrotic ringspot virus (PNRSV) / Prune dwarf virus (PDV) real-

time RT-PCRMax. 25 leaves COMBIOOQ € 68,80

Combi PDV/PNRSV/PPV Max. 25 leaves COMBIPPPQ € 81,30

Tobacco rattle virus (TRV) real-time RT-PCR Max. 25 leaves TRVQ € 68,80

Cucumber mosaic virus (CMV) real-time RT-PCR Max. 25 leaves CMVQ € 68,60

Combi CMV/TRV Max. 25 leaves COMBICTQ € 81,30

Combination apple viruses (ApMV ACLSV ASGV ASPV) RT-PCR Max. 10 leaves/leaf buds COMBI APPEL PCR € 90,80

CymMV, ORSV, Combi OFV real-time RT-PCR Max. 25 leaves COMBICOOQ € 81,30

Orchid fleck virus (OFV) real-time RT-PCR Max. 25 leaves OFVP € 68,80

CymMV, ORSV real-time RT-PCR max. 25 leaves COMBICOQ € 68,80

Bacopa chlorosis virus (BaCV) real-time RT-PCR max. 25 leaves BACVQ € 68,80

Verbena latent virus (VeLV) real-time RT-PCR max. 25 leaves VELVQ € 68,80

Phytoplasma, for material without symptomsMax. 5 stem parts, root parts

or leaf veinFYTOQ000 € 109,20 € 64,60

Fungi Sample size Test code Basic testIF/ microscope/


Fusarium oxysporum max. 6 stem parts FOA000 € 30,00 € 97,40

Test for Verticillium dahliae in soil samples 2 - 2.5 litres VDAHLA000 € 120,50

Fusarium foetens, for material without symptoms Max. 5 stem parts FFA000 € 80,60 € 64,60 € 64,60

Phytophthora ramorum Max. 20 leaves PRQ000 € 33,60

Combination Phytophthora ramorum and P. kernoviae Max. 20 leaves COMBIPRKQ000 € 52,20

Colletotrichum spp blottermethode 200 seeds CSF002 € 155,90


Bacteria Sample size Test code Basic testIF/ microscope/


Agrobacterium tumefaciens 0,5 liter water ATUMP € 142,00 € 64,60

Agrobacterium tumefaciens Max. 5 stem parts ATUMQ € 44,00

Rhodococcus facians Max. 10 stem parts RFASQ € 44,00

Combination A. tumefaciens and R. facians Max. 5 stem parts COMBIARQ € 60,00

Erwinia chrysanthemi Max. 5 stem parts ERCHRI € 31,60 € 108,20

Erwinia amylovora (latent) 100 twigs ERAMYS000 € 110,30 € 49,30 € 64,60

Xanthomonas axonopodis pv. dieffenbachiae Anthurium Max. 5 leaves+leaf stems XADIEI € 23,60 € 52,90 € 108,20

Xanthomonas campestris strain pathogenic for Lobelia Max. 5 stem parts XCLOBI € 23,60 € 51,70

Xanthomonas hortorum pv. pelargonii Max. 5 stem parts XHPELI € 23,60 € 108,20

Xylella fastidiosaMixed sample of 100 to 200

leaves or twigs XYLEFAQ000 € 160,50

Max. 5 stem parts RSOLA € 36,90 € 64,60

water 1 liter RSOLA € 36,90 € 64,60

Ralstonia solanacearum max. 200 stemparts RSOLA € 36,90 € 64,60

Other Rate

Rate per hour for extra research (if needed) € 125,10

Urgency rate € 72,80

Samples tested by Netherlands Food and Consumer Product Safety Authority € 125,10

7 As follow-up of the bio-PCR an extra PCR can be performed € 64,60

Ralstonia solanacearum culture method 7


Test rates

Health Testing of Vegetable Crops

Seed and plant material can be tested for the presence of bacterial diseases and phytoplasms. Methods that are used are e.g., plating, pathogenicity tests, immunofluorescence (IF) and PCR.

Testing for fungi is usually done by a plating method, combined with identification by microscope. New DNA techniques are used for sensitive detection of e.g. Fusarium species. Testing for fungi can

also be done on soil samples, e.g. for Verticillium dahliae and Fusarium oxysporum f.sp. asparagi.

Plant material and/or seed can be tested for the presence of viruses or viroids, using molecular techniques or ELISA.

Testing for bacteria and phytoplasms

Testing for fungi

Virus tests and viroids

If confirmation tests have to be carried out, they are added to the rates of the basic test. Where combination testing is applicable, you can find the relevant rates with the individual tests.

Laboratories Page 10 / Health in vegetable crops Tarievenlijst_ENG juni 2020

Tests Crop / Pathogen Sample size Test code Basic test IF / Microsc. PCR Bio test

Asparagus

Pathogene Fusarium spp. for asparagus 300 seeds FOAA003 € 119,50 € 111,90 € 111,90

On soil samples 2.5 - 5 liter soil FOAB000 € 123,50

Aubergine

3,000 seeds PEPMVE030 € 119,50 € 64,60

4,750 seeds PEPMVE048 € 172,70 € 64,60

Vertillium dahliae 200 seeds VDA002 € 119,50

3,000 seeds COMBIPOSPIQ030PA € 171,70

4,800 seeds (12 x 400) COMBIPOSPIQ048PA € 579,30

20,000 seeds (50 x 400) COMBIPOSPIQ200PA € 2.406,90

Tomato mottle mosaic virus (ToMMV) 20,000 seeds (50 x 400) TOMMVQ200PA € 2.297,60

Basil

Fusarium oxysporum 200 seeds FOA002BA € 90,80 € 96,30

Bean

Xanthomonas axonopodis pv. phaseoli and

Xanthomonas fuscans subsp. Fuscans culture method

Pseudomonas savastanoi pv. phaseolicola (PSP) 5,000 seeds PSPA050 € 164,80 € 64,60

Pseudomonas syringae pv. syringae (PSS) 5,000 seeds PSSA050 € 201,30 € 108,20

Pseudomonas syringae pv. syringae (PSS) 30,000 seeds PSSA300 € 400,00 € 108,20

Curtobacterium flaccumfaciens pv. flaccumfaciens 5,000 seeds CFFA050 € 163,60 € 64,60

Combination tests: XAXF - PSP – PSS 5,000 seeds

2 pathogens 8

PSS - PSP 5,000 seeds COMBIPPA050 € 283,10

PSS - XAXF 5,000 seeds COMBIPXA050 € 283,10

XAXF - PSP 5,000 seeds COMBIXPA050 € 283,10

3 pathogens 8

5,000 seeds COMBIXPPA050 € 446,80



XAP/XFF - PSP - CFF 5.000 zaden COMBIXPCA050 € 446,80

4 pathogens 8

XAXF - PSP - PSS - CFF 5,000 seeds COMBIXPPCA050 € 608,40

Bean common mosaic virus (BCMV) 400 seeds BCMVE004 € 233,10

Ascochyta spp. 200 seeds ASSA002 € 125,60

Colletotrichum lindemuthianum 200 seeds CLF002 € 155,90

Beetroot

200 seeds PBA002 € 105,50

400 seeds PBA004 € 161,50

1,000 seeds PBA010 € 271,30

Phoma betae

Pepino mosaic virus (PepMV)

5,000 seeds XAXFA050 € 155,90 € 64,60

Pospiviroids (CEVd, CLVd, MPVd, PCFVd, PSTVd, TASVd, TCDVd and

TPMVd)

XAXF - PSP – PSS

8 Confirmation tests will be charged per test € 60,70 / € 103,00



Cabbage and Radish

400 seeds PLF004 € 116,30

1,000 seeds PLF010 € 175,90

3,000 seeds PLF030 € 318,20

10,000 seeds PLF100 € 1.049,30

Plasmodiophora brassicae 2.5 liter soil PLBKB000 € 155,90

200 seeds COMBIABBA002 € 119,50

400 seeds COMBIABBA004 € 261,60

Pseudomonas syringae pv. maculicola (PSM) ZE-PCR 30,000 seeds PSMQ300 € 392,40

2,500 untreated seeds PSMA025 € 138,80 € 64,60

7,500 untreated seeds PSMA075 € 154,90 € 64,60

10,000 untreated seeds PSMA100-4SUBS € 158,70 € 64,60

10,000 treated seeds PSMA100B € 177,60 € 64,60

30,000 treated seeds PSMA300B € 222,90 € 64,60

30,000 seeds untreated seeds COMBIXXQ300 € 392,40

50,000 seeds untreated seeds COMBIXXQ500 € 604,70

Combi toets XCC en XCR en PSM DNA zaad-extract real-time PCR 30,000 seeds untreated seeds COMBIXXPQ300 € 454,20

Pseudomonas syringae pv. maculicola (PSM)

Phoma lingam

Combitoets XCCQ en XCRQ real-time PCR

Alternaria brassicae and Alternaria brassicicola Combination test



Combination tests:

10,000 untreated seeds XXAS100 € 189,00 € 64,60 € 105,50




10,000 treated seeds XXAS100B € 234,50 € 64,60 € 105,50




10,000 treated seeds COMBIXXPA100B € 293,20 € 64,60 € 105,50

30,000 treated seeds COMBIXXPA300B € 329,70 € 64,60 € 105,50

Plasmodiaphora brassicae 200 seeds PLBKB001 € 270,30

Alternaria japonica 200 seeds AJA002 € 304,70

Combi XCC/XCR en PSM ZE-PCR 30,000 seeds COMBIXXPQ300 € 454,20

Carrot

10,000 seeds XHCARA100 € 163,00 € 108,20

20,000 seeds XHCARA200 € 324,00 € 108,20

Alternaria dauci 200 seeds ALDF002 € 83,90

Alternaria radicina 200 seeds ALRF002 € 83,90

200 seeds COMBIAAF002 € 102,70

400 seeds COMBIAAF004 € 120,60

Candidatus liberibacter solanacearum 10.000 seeds CLSQ100 € 180,40

20.000 seeds CLSQ200 € 286,30

30.000 seeds CSLQ300 € 392,40

50.000 seeds CSLQ500 € 676,70

Xanthomonas campestris pv. raphani (XCA) en Xanthomonas campestris pv. campestris (XCC)

(XCA synoniem: Xanthomonas campetris pv. campestris (XCR) ) Pseudomonas syringae pv. maculicola (PSM)

Combination test XCA and XCC (seeds tretaed with warm water)

Combination test XCA, XCC and PSM

Combination test XCA - XCC (untreated seeds)

Alternaria dauci and Alternaria radicina combination Blotter method

Xanthomonas hortorum pv. carotae



Celery

200 seeds SAF002 € 97,00

400 seeds SAF004 € 128,80

10,000 seedsSAAF100

€ 60,70

20,000 seeds SAAF200 € 109,20

Phoma apiicola blotter method 1,000 seeds PAF010 € 175,90

Candidatus liberibacter solanacearum 10,000 seeds CLSQ100 € 180,40

20,000 seeds CLSQ200 € 286,30

30,000 seeds CLSQ300 € 392,40

50,000 seeds CLSQ500 € 676,70

Cucurbitaceae

10,000 seeds ACB100 € 343,50 € 64,60 € 105,50

20,000 seeds ACB200 € 684,30 € 64,60 € 105,50

30,000 seeds ACB300 € 1.026,30 € 64,60 € 105,50

10,000 seeds ACQ100 € 180,40

20,000 seeds ACQ200 € 286,30

30,000 seeds ACQ300 € 392,40

40,000 seeds ACQ400 € 498,60

50,000 seeds ACQ500 € 604,70

60,000 seeds ACQ600 € 710,70

300 seeds XCQ003 € 158,30

10,000 seeds XCQ100 € 180,40

* Extra costs for disinfection by Naktuinbouw € 18,15

9 If positive a grow out is recommended to confirm

Septoria apiicola blotter method

Acidovorax citrulli - grow out (performed with treated seeds *)

Acidovorax citrulli DNA - Real time - PCR (moleculair detection) 9

Performed with untreated seeds

Xanthomonas cucurbitae real-time PCR

Septoria apiicola soak method



10,000 seeds COMBIAXQ100 € 242,90

300 leaves CGMMVE000 € 139,50

2,000 seeds CGMMVE020 € 152,30

4,000 seeds CGMMVE040 € 258,40

6,000 seeds CGMMVE060 € 361,70

8,000 seeds CGMMVE080 € 466,80

10,000 seeds CGMMVE100 € 570,70

Cucumber green mottle mosaic virus (CGMMV) bio assay 2,000 seeds CGMMVB020 € 215,50

Cucumber mosaic virus (CMV) 2,000 seeds CMVE020 € 152,30

Cucumber leaf spot virus (CLSV) 1,000 seeds CLSVE010 € 119,50

Melon necrotic spot virus (MNSV) 2,000 seeds MNSVE020 € 152,30

Squash mosaic virus (SqMV) 2,000 seeds SQMVE020 € 152,30

Squash mosaic virus (SqMV) 8,000 seeds SQMVE080 € 466,80

Arabis mosaic virus (ArMV) 2,000 seeds ARMVE020 € 119,00

Combination tests virus: CGMMV, CMV, KGMMV, MNSV, SqMV, ZGMMV

2 viruses: 2,000 seeds € 262,70

10,000 seeds € 988,10

3 viruses: 2,000 seeds € 382,20

4,000 seeds € 748,40

6,000 seeds € 1.117,30

8,000 seeds € 1.430,20

10,000 seeds € 1.696,60

4 viruses: 2,000 seeds € 446,80

10,000 seeds € 1.686,00

5 viruses : 2,000 seeds € 509,90

10,000 seeds € 1.923,50

6 viruses : 2,000 seeds € 573,00

Cucumber green mottle mosaic virus (CGMMV)

Combination Acidovorax citrulli and Xanthomonas cucurbitae real-time

PCR



Fusarium oxysporum and Fusarium solani Combi 200 seeds FOSA002 € 180,90 € 181,60

Zucchini yellow mosaic virus 2.000 seeds ZYMVE020 € 152,30

Colletotrichum orbiculare 200 seeds COF002 € 127,20

Verticillium dahliae 200 seeds VDA002 € 119,50

Pseudomonas syringae pv. lachrymans 10,000 seeds PSLQ100 € 180,40

Combi Acidovorax citrulli en Pseudomonas syringae pv. lachrymans 10,000 seedsCOMBIAPQ100

€ 242,80

Combi Xanthomonas cucurbitae en Pseudomonas syringae pv.

lachrymans10,000 seeds

COMBIPXQ100€ 242,80

Combi Acidovorax citrulli en Pseudomonas syringae pv. lachrymans 10,000 seedsCOMBIAPXQ100

€ 305,40

Fennel


20,000 seeds CLSQ200 € 286,30

30,000 seeds CLSQ300 € 392,40

50,000 seeds CLSQ500 € 676,70

Lambs Lettuce

400 seeds PVB004 € 100,50 € 63,60

1,200 seeds PVB012 € 292,30 € 36,60

Peronospora valerianellaea shaking method 1,000 seeds PVAF010 € 106,10

Phoma valerianella blotter method 200 seeds PVF002 € 81,30

Leek

Pseudomonas syringae pv. Porri 5,000 seeds PSPOA050 € 175,90 € 64,60

Alternaria porri 200 seeds APF002 € 109,20

Leek Yellow Stripe virus (LYSV) max. 10 leaf fragments LYSVP € 119,50

Peronospora valerianellae bio test



Lettuce

10,000 seeds LMVE100 € 338,10 € 64,60

30,000 seeds LMVE300 € 343,50 € 64,60

2,000 seeds LMVE020 € 140,00 € 64,60

4,000 seeds LMVEZAAI040 € 219,60 € 64,60

6,000 seeds LMVE060 € 234,60 € 64,60

10,000 seeds LMVEZAAI100 € 437,00 € 64,60

Fusarium spp. 200 seeds FOA002SL € 90,30

Septoria spp. Blotter method 200 seeds SSF002 € 80,60

Septoria spp. Shaking method 10,000 seeds SSAF100 € 60,70

Onion

Botrytis allii 400 seeds BAA004 € 180,90

Parsley

200 seeds SPF002 € 102,40

400 seeds SPF004 € 149,90

Septoria petroselini shaking method 10,000 seeds SPAF100 € 60,70


20,000 seeds CLSQ200 € 286,30

30,000 seeds CLSQ300 € 392,40

50,000 seeds CLSQ500 € 676,70

Pea

Pseudomonas syringae pv. pisi 5,000 seeds PSPIA050 € 154,90 € 64,60

15,000 seeds PSPIA150 € 186,80 € 64,60

Lettuce mosaic virus (LMV)

Septoria petroselini blotter method

Lettuce mosaicvirus (LMV) Elisa seedlings



Pea seed-borne mosaic virus (PSbMV) 2,000 seeds PSBMVE020 € 152,30

Pea early browning virus (PEBV) 2,000 seeds PEBVE020 € 152,30

Combination test PSbMV and PEBV 2,000 seeds COMBIPPE020 € 209,30

Ascochyta spp. 2,000 seeds ASSA002 € 125,60

Fusarium oxysporum 200 seeds FOA002ER € 102,70 € 96,30

Pepper

Xanthomonas campestris pv. vesicatoria (XCV) 10,000 seeds XCVA100PA € 171,20 € 64,60

X.gardneri, X.euvesicatoria, X.perforans and X.vesicatoria 20,000 seeds XCVA200PA € 181,50 € 64,60

30,000 seeds XCVA300PA € 199,70 € 64,60

40,000 seeds XCVA400PA € 232,20 € 64,60

50,000 seeds XCVA500PA € 271,30 € 64,60

60,000 seeds XCVA600PA € 317,80 € 64,60

Erwinia carotovora subsp. carotovora

Synonym: Pectobacterium carotovorum

Tobamovirus Bio assay 3,000 seeds TOBAMOB030 € 172,70

Combination test ToMV-DO3, TMV, PMMoV ELISA 3,000 seeds COMBITOBAMOEPA € 164,60




3,000 seeds TOBRFVQ030PA € 159,90

20,000 seeds (50 x 400) TOBRFVQ200PA € 2.297,60

Tomato mottle mosaic virus (ToMMV) 20,000 seeds (50 x 400) TOMMVQ200PA € 2.297,60

3,000 seeds PSTVDQ030PA € 159,90

4,800 seeds (12 x 400) PSTVDQ048PA € 552,90

20,000 seeds (50 x 400) PSTVDQ200PA € 2.297,60

3,000 seeds COMBIPTQ030PA € 233,80

20,000 seeds (50 x 400) COMBIPTQ200PA € 2.758,00

Combi PSTVd and ToBRFV real-time RT-PCR 3.000 seeds COMBIPSTOQ030PA € 222,00

Combi ToMMV, ToBRFV and Pospiviroids real-time RT-PCR 20,000 seeds (50 x 400) COMBIPTTQ200PA € 3.218,40


ECCA100 € 64,60

Combi Pospiviroids and ToBRFV

10,000 seeds

PSTVd, TCDVd, TPMVd real-time RT-PCR

Tomato brown rugose fruit virus (ToBRFV)


TPMVd)

€ 217,90



Spinach

Cucumber mosaic virus (CMV) 2,000 seeds CMVE020SP € 152,30

Fusarium oxysporum 400 seeds FOA004SP € 128,10 € 97,40

Phomopsis spp 30,000 seeds PSV300 € 53,30 € 98,00

Cladosporium variabile 200 seeds CVF002 € 109,20

Colletotrichum dematium 200 seeds CDF002 € 80,10

Stemphylium botryosum 200 seeds SBF002 € 109,20

Verticillium spp. 200 seeds VSF002 € 109,20


Peronospora farinosa 1,000 seeds PFAF010 € 106,10

Combination tests:

Colletotrichum dematium and Verticillium spp. 200 seeds COMBIVCO € 164,10

Stemphylium botryosum and Verticillium spp. 200 seeds COMBIVS € 152,90

Stemphylium botryosum, Verticillium dahliae and Cladosporium var. 200 seeds COMBIVCS € 204,00

Stemphylium botryosum, Verticillium dahliae, Cladosporium var. and

Colletotrichum dematium200 seeds COMBIVCSC € 222,90

Sticky Nightshade (Solanum sisymbriifolium)




Strawberry

Xanthomonas fragariae max. 30 leaves XFRAQ000 € 133,40

Colletotrichum aculatum 200 seeds CAF002 € 127,20

Combination Strawberryviruses (SCV,SVBV,SMoV, SMYEV) max. 10 leavesCOMBI AARDBEI PCR

€ 90,80

Verticilium species VSA000 € 64,60

Erwinia pyrifoliae max. 30 leaves EPQ000 € 133,40

Combi Xanthomonas fragariae + Erwinia pyrifoliae max. 30 leaves COMBIXEQ000 € 159,10

Sunflower (Helianthus)

Sclerotinia sclerotiorum 200 seeds SCSA002 € 102,70

6 Tests are carried out by the Diagnostics team with priority; an urgency rate will apply. The rate for urgent assignments will apply.


TPMVd)



Tomato

10,000 seeds CMMA100 € 217,90 € 64,60 € 161,50

20,000 seeds CMMA200 € 247,30 € 64,60 € 161,50

30,000 seeds CMMA300 € 270,30 € 64,60 € 161,50

50,000 seeds CMMA500 € 341,20 € 64,60 € 161,50

60,000 seeds CMMA600 € 364,20 € 64,60 € 161,50

10,000 seeds CMMQ100 € 180,40

30,000 seeds CMMQ300 € 217,90

Clavibacter michiganensis subsp. michiganensis real-time PCR 10,000 seeds COMBICMMQA100 € 387,20 € 64,60 € 161,50

and culture methode 30,000 seeds COMBICMMQA300 € 350,40 € 64,60 € 161,50

10,000 seeds XCVA100 € 211,60 € 64,60

30,000 seeds XCVA300 € 256,30 € 64,60

10,000 seeds PSTA100 € 217,90 € 64,60

30,000 seeds PSTA300 € 248,10 € 64,60

Combination test for detection of CMM, XCV and PST 2 pathogens 10

PST / XCV 10,000 seeds COMBIPXA100 € 346,70

CMM / XCV 10,000 seeds COMBICXA100 € 346,70

CMM / XVV 20,000 seeds COMBICXA200 € 384,00

CMM / XCV 30,000 seeds COMBICXA300 € 417,30

PST / XCV 30,000 seeds COMBIPXA300 € 417,30

Combination test for detection of CMM, XCV and PST 3 pathogens 10

10,000 seeds COMBICPXA100 € 423,70




60,000 seeds COMBICPXA600 € 1.022,60

100,000 seeds COMBICPXA1000 € 1.281,60

Pseudomonas syringae pv. tomato (PST)

CMM / PST / XCV

10 Possible extra charge: Pathogenicity test CMM € 161,50 ; PCR per pathogen € 64,60

Clavibacter michiganensis subsp. michiganensis (CMM)

Clavibacter michiganensis subsp. michiganensis real-time PCR

Xanthomonas campestris pv. vesicatoria (XCV)



Tomato continued

Erwinia carotovora subsp. carotovora

Synonym: Pectobacterium carotovorum

Combitest detection of TMV-WU1, ToMV-D03, PepMV 3,000 seeds COMBIPTTE030 € 209,00

Combitest ToMV-D03, TMV 3,000 seeds COMBITOBAMOE € 164,60

Combitest ToMV-D03, TMV 20,000 seeds COMBITOBAMOE200 € 977,50

Tobamovirus Bio assay 3,000 seeds TOBAMOB030 € 172,70

Combi test ToMV-D03, TMV 3,000 seeds COMBITOBAMOE € 172,90

300 leaves PEPMVE000 € 139,50

3,000 seeds PEPMVE030 € 119,50 € 64,60

4,750 seeds PEPMVE048 € 172,80 € 64,60

Pelargonium zonale spot virus (PZSV) ELISA 3.000 zaden PZSVE030 € 151,10

3,000 seeds PSTVDQ030 € 159,90

4,800 seeds (12x 400) PSTVDQ048 € 552,90

20,000 seeds (50x 400) PSTVDQ200 € 2.297,60

200 leaves (8x 25) COMBIPOSPISCREEN € 389,80

3,000 seeds COMBIPOSPIQ030 € 171,70

4,800 seeds (12x 400) COMBIPOSPIQ048 € 579,30

20,000 seeds (50x 400) COMBIPOSPIQ200 € 2.406,90

3,000 seeds TOBRFVQ030 € 159,90

20,000 seeds (50x 400) TOBRFVQ200 € 2.297,60

Tomato yellow leaf curl virus (TYLCV) 3,000 seeds TYLCVQ030 € 159,90

Tomato mottle mosaic virus (ToMMV) 20,000 seeds (50 x 400) TOMMVQ200 € 2.297,60

3,000 seeds COMBIPTQ030 € 233,80

20,000 seeds (50x 400) COMBIPTQ200 € 2.758,00

Combi PSTVd and ToBRFV 3.000 seeds COMBIPSTOQ030 € 222,00

Combi ToMMV, ToBRFV and Pospiviroids real-time RT-PCR 20,000 seeds (50 x 400) COMBIPTTQ200 € 3.218,40

Tomato black ring virus (TBRV) 3,000 seeds TBRVE030 € 119,50

Tomato bushy stunt virus (TBSV) 3,000 seeds TBSVE030 € 119,50

200 seeds / 400 seeds FOA002TO € 90,30 € 97,40

400 seeds FOA004TO € 128,10 € 97,40

Verticilium dahliae 200 seeds VDA002 € 119,50

Alternaria solani blotter method 200 seeds ASF002 € 109,20

Didymella lycopersici 200 seeds DLA002 € 127,20

Other

Rate per hour for extra research (if needed) € 125,10


Samples tested by Netherlands Food and Consumer Product Safety Authority € 125,10

Special tests by Netherlands Food and Consumer Product Safety Authority € 198,30

Fusarium oxysporum

PSTVd, TCDVd, TPMVd real-time RT-PCR

Tomato brown rugose fruit virus (ToBRFV)

Pepino mosaic virus (PepMV)

Pospi viroids (CEVd, CLVd, MPVd, PCFVd, PSTVd, TASVd, TCDVd

and TPMVd)

Combi Pospiviroïden en ToBRFV

10,000 seeds ECCA100 € 217,90 € 64,60


For seed material to be exported to third countries, the country of destination sometimes sets additional requirements.

In order to facilitate export of your material Naktuinbouw has a number of tests available specifically for this purpose.

Gewas Pathogeen Monsteromvang Toetscode Toetstarief

Aubergine Tomato ringspot virus 3,000 seeds 9-ToRSVE030 € 140,00

Beetroot Arabis mosaic virus 3,000 seeds 9-ARMVE030 € 140,00

Heterodera schachtii 100 gram seeds Tested at NPPO € 198,30

Carrot Fusarium oxysporum 200 seeds 9-FOA002 € 130,00

Phoma sp. 200 seeds 9-PSF002 € 130,00

Celery Strawberry latent ringspot virus (SLRSV) 3,000 seeds 9-SLRSVE030 € 140,00

Cucurbitaceae Tomato ringspot virus (ToRSV) 2,000 seeds 9-ToRSVE020 € 172,00

Alternaria cucumerina 200 seeds 9-ACA002 € 130,00

Tobacco ringspot virus (TRSV) 2,000 seeds 9-TRSVE020 € 172,00

Lettuce Arabis mosaic virus (ArMV) 3,000 seeds 9-ARMVE030 € 140,00

Tomato ringspot virus (ToRSV) 3,000 seeds 9-ToRSVE030 € 140,00


Verticillium sp. 200 seeds 9-VSF002 € 130,00

Onion Onion yellow dwarf virus (OYDV) 3,000 seeds 9-OYDVE030 € 140,00

Tobacco rattle virus (TRV) 3,000 seeds 9-TRVE030 € 140,00

Tobacco streak virus (TSV) 3,000 seeds 9-TSVE030 € 140,00

Pepper Arabis mosaic virus (ArMV) 3,000 seeds 9-ARMVE030 € 140,00

Tobacco rattle virus (TRV) 3,000 seeds 9-TRVE030 € 140,00



Tomato Arabis mosaic virus (ArMV) 3,000 seeds 9-ARMVE030 € 140,00


Tobacco streak virus (TSV) 3,000 seeds 9-TSVE030 € 140,00


Other


Tests for the purpose of export requirements third countries

Laboratories Page 22 / Export requirements Tarievenlijst_ENG juni 2020

Sample size Rate

400 seeds € 58,90

400 seeds € 58,90

300 seeds € 51,30

200 seeds € 45,00

100 seeds € 38,90

Sample size Rate

400 seeds € 199,40

Sample size Rate

Betula 1 gram € 107,20

Sample size Rate

400 seeds € 107,20

300 seeds € 100,90

200 seeds € 94,30

100 seeds € 87,40

Usable Plant test

Naktuinbouw certificate

Seed Quality Analysis

Test rates

Germination test (tree and palm seeds)


ISTA certificate 11

/ Naktuinbouw certificate

Germination on weight

11 For ISTA certificates costs for sampling and certificates are billed separately.

The Seed Analysis Laboratory carries out various tests to assess the quality of seed of flowers, trees, vegetables and herbs. Tests for

germination, purity and moisture content or for tetrazolium and weeds are carried out under ISTA accreditation. In addition, the more

practice-related Usable Plants Test can be performed. This can be applied to crops such as tomato, sweet pepper, cucumber-like crops,

lettuce and cabbage.

ISTA certificate 11

/ Naktuinbouw certificate

ISTA certificate 11

Germination test (excluding tree and palm seeds)

Laboratories Page 23 / Seed Quality Analysis Tarievenlijst_ENG juni 2020

Sample size Rate

400 seeds € 232,30

400 seeds € 232,30

200 seeds € 191,30

100 seeds € 109,60

Sample size Rate

400 seeds € 393,60

400 seeds € 393,60

200 seeds € 347,70

100 seeds € 206,00

Sample size Rate

400 seeds € 58,90

300 seeds € 51,30

200 seeds € 45,00

200 seeds € 22,80

400 seeds € 58,90

300 seeds € 51,30

200 seeds € 45,00

200 seeds € 57,70

upon request

Sample size Rate

800 seeds € 26,10

800 seeds € 26,10

400 seeds € 18,50

300 seeds € 15,90

200 seeds € 13,20

ISTA certificate 11

Tetrazolium test (excluding tree and palm seeds)

11 For ISTA certificates costs for sampling and certificates are billed separately.

ISTA certificate 11

Root length determination (spinach)

ISTA certificate 11

Accelerated Aging Test

Conductancy test beans or peas (excl. moisture determination)



Cold test (maize)

Root resignation (beetroot and corn)


Tetrazolium test (tree and palm seeds)

Thousand seed weight (These tests are done on clean seeds)

Vigour tests (Naktuinbouw certificate)


Sample size Rate

50 or 100 gram 23,50

25 gram 23,50

Sample size Rate

Pulses 2,500 seeds € 33,00

2,500 seeds € 46,60

25,000 seeds € 201,70

25,000 seeds € 169,60

€ 27,10

Various (Naktuinbouw certificate) Sample size Rate

5 seeds € 26,60

10 bulbs/roots € 26,10

Determining ‘single seeds rate’ of beet seeds 400 seeds € 48,70

20 seeds € 40,00

Fractioning test 25,000 seeds

500 gram seeds € 26,60

25,000 seeds € 51,30

€ 27,10

€ 18,70

€ 72,80

€ 10,20

€ 31,70

€ 4,20

€ 125,1011

For ISTA certificates costs for sampling and certificates are charged separately.

Rate per hour for extra research (if needed)

Determining the presence of other seeds

Purtity test and determination of other seeds export Australia (combination test)

Other

Alteration on a Naktuinbouw certificate

Alteration on an ISTA certificate

Surcharge priority treatment

ISTA certificate 11

Purity test (ISTA-certificate 11

/ Naktuinbouw-certificate)

Moisture determination

Purity

Other

Extra charges

Creation of an ISTA certificate

Determining ploidy level

Determining the presence of beetles in seed lots

Determining the presence of Sclerotinia

Determining the presence of Rhizomanie

Disinfection of seeds before testing


upon request

upon request

Determining the presence of broken, discoloured etc. seeds (‘piksel’)

Seed determination

Determining dry matter of onion/carrot

Determining the kind of starch for pea


Identity testing

Rates for SSR identification

For 1 - 4 Samples

For 5 - 8 Samples

For 9 - 32 Samples

For more samples, we would gladly provide you with a quotation.

Rate for standard AFLP projects

For 1-16 samples with 4 primer combinations € 2.830

For other amounts of samples (17-32 or 33-64) and/or other amounts of primer combinations, we would gladly provide you with a quotation.

Variety Tracer

Molecular identification of Cmm / Xcc isolates.

Sample selection for AFLP projects are the responsibility of the commissioning party. If Naktuinbouw feels that the selection of reference varieties and/or the number of primer combinations are inadequate, no report will be

issued until further testing. Unless the commissioning party is requesting a specific set of primer combinations for testing and analyses, it is up to Naktuinbouw to choose the primer combinations which will be tested.

Variety Identification

Naktuinbouw has laboratory techniques to determine the identity of your material. This may be important and useful where material has been mixed, mistakenly replaced, origin tracing (pathogens), when the matter is urgent

and/or when morphological research is not possible.

Identity testing is carried out by using DNA fingerprint techniques. A typical research project includes sampling, DNA isolation, fingerprinting, analysis, determination of the genetic relations, statistical analysis and reporting.

It is important for identity testing that the commissioning party provides the samples as well as adequate reference samples. If samples need to be compared to a Naktuinbouw database with varieties from a third party,

Naktuinbouw has to and will ask for permission from said parties before a report can be issued.

For the crops; Strawberry, Phalaenopsis, Apple, Potato and Rose Naktuinbouw features a SSR-Database. For these varieties it is possible to do an identification project per sample.

€ 274,50 per sample



Naktuinbouw developed a concept to help with settling the allegations of infringement of plant breeders' rights and patents: Naktuinbouw Variety Tracer®. It can provide evidence in cases of alleged infringement of plant

breeders' rights, it allows to determine the identity of varieties and it can confirm suspicion of repeated cropping. Naktuinbouw Variety Tracer combines knowledge, independence and the possibility of morphological research

with the possibilities of DNA fingerprint techniques. It is the combination of – and synergy between – these elements that guarantees extremely sound analyses and provides important evidence for possible court cases.

Please contact us for more information and a quotation.

The molecular analysis of isolates of plant pathogenic bacteria will give insight in the genetic similarity. This will be done by comparing the DNA profiles from the supplied samples amongst each other and to profiles stored in

the database. The results of the analysis can give insight into the relationship between the occurrences of different isolates. The analysis will be performed on a single colony culture. These can be supplied by you,

accompanied with a laboratory submission form. A combination with a diagnostic test is also possible if bacteria are found in symptomatic material. A single colony culture will be made on which the molecular analysis and

comparison will be performed. The results will be provided in a report including a dendrogram.

Laboratories Page 26 / Variety Identification Tarievenlijst_ENG juni 2020

Standard rate per isolate: € 301,00

Urgency rate: € 1.990,30 (excluding the rate per isolate of € 301,00)

The analysis will commence directly upon receiving the samples. Completion of the analysis and report may take up to 3 weeks.

For other organisms we would gladly provide you with a quotation.

Other services

DNA Isolation

1 plate, CTAB extraction (1 - 94 samples) € 316,70

2 plates, CTAB extraction (95 - 188 samples) € 475,00

AFLP, Tiff files

It's posible to receive a Tiff file of a primer combination without analysis.

1 gel project with 1 primer combination € 1.002,00

1 gel project with 2 primer combinations € 1.267,00

Up to a minimum of 32 samples will be collected before the analysis will be started. If there are less than 32 samples the analysis will start within 3 months. Completion of the analysis and report may take up to 3 weeks.

*Rates are based on 1-64 samples and without DNA isolation

Laboratories Page 27 / Variety Identification Tarievenlijst_ENG juni 2020

Bio assays and making crops free from viruses

Composition of the test rate

Testing fees

Tests:

Sample size Rate

min. 1 g. leafs € 205,90

min. 1 g. leafs € 45,50

min. 1 g. leafs € 143,40

€ 216,40

€ 216,40

€ 88,80

€ 342,00

€ 150,00

€ 160,60

€ 90,80

€ 110,40

€ 72,00

Rendering free of viruses; woody plants Making woody plants virus-free:

(Including testing program, heat treatement if necessary, excluding identity determination) Sample size Rate

€ 3.853,50

€ 3.220,00

Malus, Pyrus (per variety per year, maximum 5 years) Sample size Rate

year 1 (pre-testing, heat treatment) € 2.776,60

€ 4.919,80

€ 664,00

Arboriculture Crops and Fruits

Arboricultural crops (including apples, pears, small fruit, strawberries and roses) are made free from viruses by means of various treatments, including heat

treatment, at the Naktuinbouw Test Centre in Horst. The tops of treated plants are grafted onto virus-free rootstock or propagated from cuttings. Subsequent

laboratory analyses, liquid inoculations using test plants and grafting onto woody indicators is applied to confirm the virus-free status of the grafted tops.

year 2 (testing treated material)

Naktuinbouw charges a base rate for handling costs in addition to the test rate as indicated below.

Detection of Colletotrichum acutatum via paraquat-test

Phytopthora fragariae testing in strawberry (Duncan-test)

Combination of strawberry viruses PCR test (SCV – SVBV – SMoV – SMYEV)

Virus test of strawberry by leaf grafting on Fragaria vesca UC5, UC12,EMC, Alpinum

Virus test on woody indicator plants (in the field per indicator, per specimen)

Virus test on woody indicator plants (in the greenhouse per indicator, per specimen)

Combination Apple viruses PCR test (ApMV – ACLSV - ASGV - ASPV) 12

Virus test on Prunus serrulata ‘Shirofugen’

Virus test on two herbaceous host Saponaria, both in duplicate

Each following test plant, per single plant

Virus test on two herbaceous host, both in duplicate

The base rate for every assignment is € 51,70.

Virus test on Rubus occidentalis

year 3 up to year 5 (maintaining treated material; rate per year)

Soft fruits (Ribes, Rubus, a.o.) (per variety per year, maximum 2 years)

Strawberry (per variety)

Laboratories Page 28 / Arboriculture Crops and Fruits Tarievenlijst_ENG juni 2020

Prunus (per variety per year, maximum 4 years) Sample size Rate

€ 2.365,00

€ 4.044,00

€ 664,00

Sample size Rate

upon request

€ 931,00

Per order of woody plants and strawberry, 3 fully tested plants will be delivered.

Maintenance pre-basic and pre-nuclear stock Sample size Rate

€ 166,30

€ 73,10

Per parent line € 332,80

Consignment Sample size Rate

€ 28,50

Small fruit Sample size Rate

€ 11,30

€ 7,75

€ 124,30

Sample size Rate

Per hour € 80,75

Special services Sample size Rate

upon request

upon request

upon request

13 Rates are excluding delivery costs

Extra heat treatment

Other woody plants

year 3 and year 4 (maintaining treated material; rate per year)

year 2 (testing treated material)

year 1 (pre-testing, heat treatment)

Woody plants prebasic (fruit crops and soft fruit crops)

Delivery graft inoculation wood

Consignment SEE Root blocks 13

Consignment SEE Plug plants 13

Large fruit

Consignment SEE Cutting 13

Consignment SEE strawberry 13

Maintaining treated material pre-basis strawberry

Woody plants pre-nuclear stock (fruit crops and soft fruit crops)

DNA fingerprinting strawberry crop

Health certificate import for 2008/61 EG

Preparing material for the request for Plant Breeders’ Rights

Laboratories Page 29 / Arboriculture Crops and Fruits Tarievenlijst_ENG juni 2020

naktuinbouw laboratories price list 2020...laboratories page 4 / nematodes tarievenlijst_eng juni...

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