N-Term.STAT3(S21320/610190)

PY 705STAT3(#9131)

STAT1 (+)(sc-346)

STAT5(a+b)(sc-836)

PARP(556362)

-Actin(AC-74)

- D 10 100 250 1 10 60

AG490 z-VAD-FMK(M) (M)

B

A- D - D-UV +UV

97.1 kDa-67.3 kDa-43.9 kDa-

C-Term.

STAT3(sc-482)

97 - 72 - 45 -

29 -

97 - 72 - 45 -

97 - 72 - 45 -

29 -

C-Term.

STAT3(sc-482)

97 - 72 - 45 -

97 - 72 - 45 -

97 - 72 -

45 -

C AG490 MG132

- D 1 10 100 50 -

C-Term.

STAT3(sc-482)

Cytopl.

Nuclei

(M)

102 - 71 - 46 -

28 - 102 - 71 - 46 -

28 -W.T. JAK2+/-

102 -C-Term.

STAT3(sc-482)

71 -

46 -

28 -

+LIF -LIF +LIF -LIFD

C-Term.

STAT3(sc-482)

PY 705STAT3(#9131)

-Actin(AC-74)

121 - 86 - 69 - 52 - 40 - 28 -

E D10L D2In. D3In. D6In.

40 -

+ WT + WT + WT + WT

121 - 86 - 69 - 52 - 40 - 28 -

52 -