myelinated and unmyelinated axons: how they work and what they do 1
TRANSCRIPT
![Page 1: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/1.jpg)
Myelinated and unmyelinated axons:how they work and what they do
1
![Page 2: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/2.jpg)
Anatomy of a human nerve
Connective tissue
Axons contained in fascicles
2
![Page 3: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/3.jpg)
Anatomy of a human nerve
Fascicle in detail: wide range of axon sizes•Diameters range from 1 - 20 μm•Some myelinated, some not 3
![Page 4: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/4.jpg)
Relations between glial cells and axons1. Myelinated axons
CNS: each oligodendrocyte myelinates several axonsPNS: each Schwann cell myelinates one axon
Schwann cell spirals around axon
4
![Page 5: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/5.jpg)
2. Mammalian unmyelinated axons
Fundamental unit is the Remak bundle: one Schwann cell supporting several axons
Relations between glial cells and axons
5
![Page 6: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/6.jpg)
Faster conduction velocity for the same diameterorsmaller diameter for a given conduction velocity
Example:Frog nerve fibre: diameter 20 μm, myelinatedConduction velocity ~25 m/s at 20 °CSquid giant axon: diameter 1 mm, unmyelinatedConduction velocity ~25 m/s at 20 °CTakes up 2500 times more space!
Why myelination?
6
![Page 7: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/7.jpg)
Measuring conduction velocity in nerve
Stimulating electrodes
Recording electrodes
Conduction distance d
7
![Page 8: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/8.jpg)
Measuring conduction velocity in nerve
Conduction time t 8
![Page 9: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/9.jpg)
Measuring conduction velocity in nerve
Conduction velocity v = d / t
Conduction distance d Conduction time t
9
![Page 10: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/10.jpg)
Heterogeneous conduction velocities
Conduction velocity heterogeneity is a consequence of:•different axon diameters•myelination or notConduction velocity also relates to function... 10
![Page 11: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/11.jpg)
Relating conduction velocity to function
Group CV Diameter Functionm/s μm
SENSORY (afferent)MyelinatedAα 70-120 12-20 Muscle spindle afferentAβ 35-70 6-12 Light touch sensationAδ 5-30 1-6 Sharp pain, strong heat, cold
UnmyelinatedC 0.5-2 0.5-1 Dull pain, ache, itch, warmth,
cold
MOTOR (efferent)MyelinatedAα 70-120 12-20 Muscle contractionAγ 12-48 2-8 Muscle spindle adjustment
UnmyelinatedC 0.5-2 0.5-1 Sympathetic, parasympathetic
11
![Page 12: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/12.jpg)
How does myelinated nerve conduct?
Unmyelinated nerve Myelinated nerve
•Action potential is generated only at the nodes•Conduction between nodes is instantaneous•That’s why it is so fast
12
![Page 13: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/13.jpg)
Saltatory conduction (textbook view)
13
![Page 14: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/14.jpg)
But... how long is an action potential?
•Duration about 0.5-0.8 ms
•Conduction velocity about 50 m/s (50 mm/ms)
•So length is about 50 x 0.5-0.8 mm = 25-40 mm
•Internodal length is about 1 mm
•So during an action potential about 25-40 nodes are depolarised at any one time
•Not like this!
14
![Page 15: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/15.jpg)
What determines conduction velocity?•Current through the membrane can be measured from the outside (because currents flow in a loop)
Huxley & Stämpfli 1949
•Conduction within each internode is instantaneous
•Brief delay (~20 μs) at each node
15
![Page 16: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/16.jpg)
50
0
mV
-50
-100
0 1 2 3 4 msec
Schwarz, Reid & Bostock 1995
What determines conduction velocity?•Nodal delay in a real recording from a human nerve fibre
•The delay is the time taken to reach threshold (the subthreshold depolarisation)
•This is the time it takes to charge the membrane capacitance
Threshold
16
![Page 17: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/17.jpg)
Evidence for the importance of the node
How do we know action potentials are generated at the node?
•Threshold is low at node, infinitely high in internode•Cooling slows conduction if applied at the node, not in internode•Blockers (e.g. local anaesthetics) applied at node are effective•Blockers applied in internode have no effect
•Most importantly: inward current during an action potential appears only at the node, not in the internode(Tasaki & Takeuchi 1942, Huxley & Stämpfli 1949)•Inward current is the sign of active membrane
17
![Page 18: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/18.jpg)
Evidence for myelinated nerve conduction
First dissect a single myelinated nerve fibre…
(Schwarz, Reid & Bostock 1995)18
![Page 19: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/19.jpg)
Evidence for myelinated nerve conductionTasaki & Takeuchi 1942
19
![Page 20: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/20.jpg)
Local circuits in myelinated nerve
Huxley & Stämpfli 1949:
Air gap blocks conductionSaline bridge restores conduction
20
![Page 21: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/21.jpg)
How axons conduct: local circuit currents
21
![Page 22: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/22.jpg)
50
0
mV
-50
-100
0 1 2 3 4 Time (ms)
Schwarz, Reid & Bostock 1995
Action potential in time and space
Threshold
Depolarisation
Repolarisation
1. Time
22
![Page 23: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/23.jpg)
Depolarisation
Repolarisation
Conduction direction
Threshold
Action potential in time and space2. Space
23
![Page 24: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/24.jpg)
Local circuit currents during the AP
Na+ in
Depolarisation
K+ out
Repolarisation
Conduction
Threshold
???
24
![Page 25: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/25.jpg)
Local circuit currents during the AP
25
![Page 26: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/26.jpg)
Local circuit currents during the AP
26
![Page 27: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/27.jpg)
What do local circuit currents consist of?
- Currents in aqueous solutions consist of ions moving- Movement along the axon is limited but ions “bump into” neighbouring ions to produce the appearance of a flow from one point to another- Like “Newton’s cradle”
http://commons.wikimedia.org/wiki/File:Newtons_cradle_animation_book.gif
Local circuit currents during the AP
27
![Page 28: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/28.jpg)
What happens ahead of the AP?
Na+ ions entering during AP
K+ ions leaving during repolarisation
+ + +
– – –
...but what’s going on here?
Charge buildup across membrane…positive inside
Na+ and K+ ions “bumping” each
other along28
![Page 29: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/29.jpg)
Can we record local circuit currents ahead of the AP?
Conduction
29
![Page 30: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/30.jpg)
Local circuit currents:how can we record them?
•First need to block the AP
•Hodgkin (1937): first to record local circuit currents in blocked nerve
•What did he do?
30
![Page 31: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/31.jpg)
Local circuit currents
Stimulating electrodes
Recording electrodes
Frog sciatic nerve
•Recording setup:
Recording electrodes can move along nerve
31
![Page 32: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/32.jpg)
Action potential arriving at block
1.4 mm after block
2.5 mm after block
4.1 mm after block
5.5 mm after block
8.3 mm after block
•Here’s what Hodgkin recorded:
Local circuit currents
Hodgkin 1937
32
![Page 33: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/33.jpg)
•Plotting amplitude against distance from block:
Exponential curve
•Falls off with distance like a graded (local)potential, like synaptic potential in lecture 1•Follows an exponential curve
Local circuit currents
Why use action
potentials?
•Without action potentials, voltage falls off quickly with distance
•This is OK in a short interneurone
•Over longer distances we need a digital code
Hodgkin 1937
33
![Page 34: Myelinated and unmyelinated axons: how they work and what they do 1](https://reader036.vdocuments.mx/reader036/viewer/2022081418/56649f0c5503460f94c1f8ab/html5/thumbnails/34.jpg)
Reading for today’s lecture:
Nerve conduction and myelination•Purves et al chapter 3 (page 49 onwards)•Purves et al chapter 9 (pages 189-194)
•Nicholls et al pages 121-126See also
•“Extra material on space constant” on website
Next lectureNa+ and K+ channels
•Purves et al chapter 3 (up to page 48) and chapter 4•Nicholls et al chapter 6 pages 94-102 and chapters 2-3
•Kandel et al chapter 6