mycobacterium avium infection in a horse n07-506

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Mycobacterium avium Mycobacterium avium infection in a Horse infection in a Horse N07-506 N07-506 Ian Hawkins Ian Hawkins Presented at SEVPAC 2008 – Presented at SEVPAC 2008 – Permission granted for use on Permission granted for use on SEVPAC website only SEVPAC website only

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Mycobacterium avium infection in a Horse N07-506. Ian Hawkins. N07-506 - History. An 8 year old male QH gelding presented to the UFVMC on 8/18/07 with suspected anterior enteritis Improved on banamine and lidocaine until 8/25/07. Abdominocentesis revealed elevated WBC count and TP - PowerPoint PPT Presentation

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Page 1: Mycobacterium avium  infection in a Horse N07-506

Mycobacterium aviumMycobacterium avium infection in a Horseinfection in a Horse

N07-506N07-506

Ian HawkinsIan Hawkins

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Page 2: Mycobacterium avium  infection in a Horse N07-506

N07-506 - HistoryN07-506 - History An 8 year old male QH An 8 year old male QH

gelding presented to the gelding presented to the UFVMC on 8/18/07 with UFVMC on 8/18/07 with suspected anterior enteritissuspected anterior enteritis

Improved on banamine and Improved on banamine and lidocaine until 8/25/07.lidocaine until 8/25/07.

Abdominocentesis revealed Abdominocentesis revealed elevated WBC count and TPelevated WBC count and TP

Surgery performed on Surgery performed on 8/26/07 revealed multiple 8/26/07 revealed multiple jejunal and ileal masses and jejunal and ileal masses and necrotic section of jejunumnecrotic section of jejunum

Due to poor prognosis, the Due to poor prognosis, the patient was euthanizedpatient was euthanized

Necropsy was performed on Necropsy was performed on 8/27/078/27/07

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Page 3: Mycobacterium avium  infection in a Horse N07-506

Gross FindingsGross Findings

Gross Diagnosis:Gross Diagnosis:– Granulomatous Granulomatous

enteritis, chronic, enteritis, chronic, diffuse with diffuse with multifocal multifocal transmural abscess transmural abscess formation, small formation, small intestine. intestine.

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Page 4: Mycobacterium avium  infection in a Horse N07-506

Gross FindingsGross Findings

Gross Diagnoses:Gross Diagnoses:– Renal papillary Renal papillary

necrosis, chronic, necrosis, chronic, multifocal, marked, multifocal, marked, Kidneys. Kidneys.

– Mesenteric and Mesenteric and colonic colonic lymphadenomegalylymphadenomegaly..

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Page 5: Mycobacterium avium  infection in a Horse N07-506

Microscopic Findings – Small Microscopic Findings – Small intestineintestine

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Page 6: Mycobacterium avium  infection in a Horse N07-506

Small Intestine with Acid-Fast Small Intestine with Acid-Fast StainStain

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Page 7: Mycobacterium avium  infection in a Horse N07-506

Kidney and LiverKidney and Liver

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Page 8: Mycobacterium avium  infection in a Horse N07-506

Mesenteric lymph nodeMesenteric lymph node

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Page 9: Mycobacterium avium  infection in a Horse N07-506

DiagnosesDiagnoses Final Anatomic Diagnoses:Final Anatomic Diagnoses:

1.1. Granulomatous enteritis, chronic, multifocal, Granulomatous enteritis, chronic, multifocal, severe with abundant intralesional, acid-fast severe with abundant intralesional, acid-fast bacterial rods, small intestine.bacterial rods, small intestine.

2.2. Granulomatous lymphadenitis, chronic, Granulomatous lymphadenitis, chronic, multifocal, moderate, mesenteric lymph node.multifocal, moderate, mesenteric lymph node.

3.3. Granulomatous typhlocolitis, chronic, multifocal, Granulomatous typhlocolitis, chronic, multifocal, moderate to marked, cecum and colon.moderate to marked, cecum and colon.

4.4. Portal hepatitis, granulomatous, chronic, Portal hepatitis, granulomatous, chronic, multifocal, moderate to marked, liver.multifocal, moderate to marked, liver.

5.5. Renal papillary necrosis, chronic, multifocal, Renal papillary necrosis, chronic, multifocal, marked, kidneys.marked, kidneys.

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Page 10: Mycobacterium avium  infection in a Horse N07-506

Polymerase Chain ReactionPolymerase Chain Reaction

Paraffin-embedded sections of Paraffin-embedded sections of intestine were submitted to the NVSL intestine were submitted to the NVSL in Ames, IA and Claus Buergelt’s lab in Ames, IA and Claus Buergelt’s lab at the University of Florida – at the University of Florida – performed by Elliot Williams.performed by Elliot Williams.

Results from both labs were positive Results from both labs were positive for for Mycobacterium aviumMycobacterium avium– 16sRNA positive16sRNA positive– IS1311 positiveIS1311 positive

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Page 11: Mycobacterium avium  infection in a Horse N07-506

PCRPCR PCR using IS900 PCR using IS900

based primers for based primers for Mycobacterium Mycobacterium aviumavium ssp. ssp. paratuberculosis. paratuberculosis.

Tissue was positive Tissue was positive using J1-J2 IS900 using J1-J2 IS900 primers (nested primers (nested primers). primers).

Johne’s? or a variant Johne’s? or a variant of of M. avium M. avium ssp. ssp. paratuberculosis.paratuberculosis.

http://www.waterscan.co.yu/thumbnails/Mycobacterium%20avium.jpg

Map Map nest

N07506

Map

N07

506

Neg

Neg Neg

P90/P91 J1/J2

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Page 12: Mycobacterium avium  infection in a Horse N07-506

Mycobacterium aviumMycobacterium avium in in HorsesHorses

Infections in horses are uncommon but Infections in horses are uncommon but have been reportedhave been reported

Typically occur in younger adult horses and Typically occur in younger adult horses and predominantly affects the gastrointestinal predominantly affects the gastrointestinal tract; also the lungs and liver with less tract; also the lungs and liver with less frequencyfrequency

Clinically horses tend to have chronic Clinically horses tend to have chronic weight loss, diarrhea, and hypoproteinemia.weight loss, diarrhea, and hypoproteinemia.

M. avium M. avium has been isolated from horses has been isolated from horses with Equine Granulomatous Enteritis, a with Equine Granulomatous Enteritis, a sporadic idiopathic disease of young horses sporadic idiopathic disease of young horses with similar clinical signs and lesions. with similar clinical signs and lesions.

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Page 13: Mycobacterium avium  infection in a Horse N07-506

Mycobacterium Mycobacterium infections in infections in horseshorses

Gross findings:Gross findings:– Multifocal soft to firm Multifocal soft to firm

transmural nodules transmural nodules throughout affected throughout affected regions of the GI tractregions of the GI tract

– Ulcerated mucosaUlcerated mucosa Histopathology:Histopathology:

– Granulomatous Granulomatous inflammationinflammation

– High numbers of High numbers of multinucleated giant multinucleated giant cellscells

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Page 14: Mycobacterium avium  infection in a Horse N07-506

Mycobacterium Mycobacterium infections in infections in horseshorses

Johne’s Disease in a horse?Johne’s Disease in a horse?– Horses have been experimentally Horses have been experimentally

infected with the organism; sporadic infected with the organism; sporadic cases have been reported, but were cases have been reported, but were unconfirmed and exceedingly rare.unconfirmed and exceedingly rare.

– A variant of A variant of M. avium M. avium ssp.ssp. paratuberculosisparatuberculosis that horses maybe that horses maybe more susceptible to?more susceptible to?

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Page 15: Mycobacterium avium  infection in a Horse N07-506

AcknowlegementsAcknowlegements

Barbara Sheppard, DVM, PhD, Dipl. Barbara Sheppard, DVM, PhD, Dipl. ACVPACVP

Elliot WilliamsElliot Williams Giles R.G. MonifGiles R.G. Monif John Roberts, DVM, Dipl. ACVPJohn Roberts, DVM, Dipl. ACVP David Taylor, BVSc, Dipl. ACVPDavid Taylor, BVSc, Dipl. ACVP Fellow UF Pathology ResidentsFellow UF Pathology Residents The UFVMC Histology lab staffThe UFVMC Histology lab staff

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Page 16: Mycobacterium avium  infection in a Horse N07-506

Questions?Questions?

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