mycobacterium avium infection in a horse n07-506

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Mycobacterium avium infection in a Horse N07-506. Ian Hawkins. N07-506 - History. An 8 year old male QH gelding presented to the UFVMC on 8/18/07 with suspected anterior enteritis Improved on banamine and lidocaine until 8/25/07. Abdominocentesis revealed elevated WBC count and TP - PowerPoint PPT Presentation

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  • Mycobacterium avium infection in a HorseN07-506Ian HawkinsPresented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • N07-506 - HistoryAn 8 year old male QH gelding presented to the UFVMC on 8/18/07 with suspected anterior enteritisImproved on banamine and lidocaine until 8/25/07.Abdominocentesis revealed elevated WBC count and TPSurgery performed on 8/26/07 revealed multiple jejunal and ileal masses and necrotic section of jejunumDue to poor prognosis, the patient was euthanizedNecropsy was performed on 8/27/07Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Gross FindingsGross Diagnosis:Granulomatous enteritis, chronic, diffuse with multifocal transmural abscess formation, small intestine. Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Gross FindingsGross Diagnoses:Renal papillary necrosis, chronic, multifocal, marked, Kidneys. Mesenteric and colonic lymphadenomegaly.Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Microscopic Findings Small intestinePresented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Small Intestine with Acid-Fast StainPresented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Kidney and LiverPresented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Mesenteric lymph nodePresented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • DiagnosesFinal Anatomic Diagnoses:Granulomatous enteritis, chronic, multifocal, severe with abundant intralesional, acid-fast bacterial rods, small intestine.Granulomatous lymphadenitis, chronic, multifocal, moderate, mesenteric lymph node.Granulomatous typhlocolitis, chronic, multifocal, moderate to marked, cecum and colon.Portal hepatitis, granulomatous, chronic, multifocal, moderate to marked, liver.Renal papillary necrosis, chronic, multifocal, marked, kidneys.Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Polymerase Chain ReactionParaffin-embedded sections of intestine were submitted to the NVSL in Ames, IA and Claus Buergelts lab at the University of Florida performed by Elliot Williams.Results from both labs were positive for Mycobacterium avium16sRNA positiveIS1311 positivePresented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • PCRPCR using IS900 based primers for Mycobacterium avium ssp. paratuberculosis. Tissue was positive using J1-J2 IS900 primers (nested primers). Johnes? or a variant of M. avium ssp. paratuberculosis.http://www.waterscan.co.yu/thumbnails/Mycobacterium%20avium.jpgMapMap nestN07506MapN07506NegNeg NegP90/P91J1/J2Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Mycobacterium avium in HorsesInfections in horses are uncommon but have been reportedTypically occur in younger adult horses and predominantly affects the gastrointestinal tract; also the lungs and liver with less frequencyClinically horses tend to have chronic weight loss, diarrhea, and hypoproteinemia.M. avium has been isolated from horses with Equine Granulomatous Enteritis, a sporadic idiopathic disease of young horses with similar clinical signs and lesions.

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Mycobacterium infections in horsesGross findings:Multifocal soft to firm transmural nodules throughout affected regions of the GI tractUlcerated mucosaHistopathology:Granulomatous inflammationHigh numbers of multinucleated giant cellshttp://freelief.com/temp/april4dee.jpgPresented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Mycobacterium infections in horsesJohnes Disease in a horse?Horses have been experimentally infected with the organism; sporadic cases have been reported, but were unconfirmed and exceedingly rare.A variant of M. avium ssp. paratuberculosis that horses maybe more susceptible to?

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • AcknowlegementsBarbara Sheppard, DVM, PhD, Dipl. ACVPElliot WilliamsGiles R.G. MonifJohn Roberts, DVM, Dipl. ACVPDavid Taylor, BVSc, Dipl. ACVPFellow UF Pathology ResidentsThe UFVMC Histology lab staff

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

  • Questions?Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

    Presented at SEVPAC 2008 Permission granted for use on SEVPAC website only

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