8/7/2019 Multiple Microarray diagnostics for STDs main

1/17

Group 9

Madhunika

Emmanuel

SaadOnyedika

DNA Microarray Technology

Diagnostics of Multiple STDs

8/7/2019 Multiple Microarray diagnostics for STDs main

2/17

Background

Smart Inc is a young biotechnologycompany made up of freshbiotechnology graduates

Manufactures diagnostic tools for STDswith DNA microarray technology.

Located in the heart of Sub-Saharan

African 340 million cases a year of STDs (WHO)

8/7/2019 Multiple Microarray diagnostics for STDs main

3/17

STDs: Sexually transmitteddiseases/infections.

Different types :HIV, Gonorrhea, Chlamydia,Syphilis, Herpes Simplex Virus 1&2 etc.

No visiblesymptoms

Disorders:Infertility,meningitis,irreversible

damage tothe heart,death etc.

Diagnostic tests

Serology,microscopy andcell culture, directplasma antibody,

Elisa(enzymelinked

immunoabsorbentassay),

Immunoflurescenceetc.

8/7/2019 Multiple Microarray diagnostics for STDs main

4/17

STDs Prevalence Worldwide

Guttmacher2010

8/7/2019 Multiple Microarray diagnostics for STDs main

5/17

OPPORTUNITIES

In

Ethiopia

Adult population= 45 million Incidence rate=4.5%

The Health and economic burden of STIsare significant and increasing in Sub-Saharan Africa( Ethiopia)

Complications as a result of wrongdiagnosis

Few diagnostic tools meet the productcriteria needs of Sub-Saharan Africa

Cost effective to join the diagnostic market

8/7/2019 Multiple Microarray diagnostics for STDs main

6/17

Reason fornon-effective STI

diagnostic tools in Sub Saharan

Africa

A real (or in somecases perceived)

lack of acommercial

market

Limited publicsector advocacyor coordinationof R&D process

Time lag to getresults

Poor access toclinical trials

Poorunderstanding of

tests needed

Lack of accessto protocols

8/7/2019 Multiple Microarray diagnostics for STDs main

7/17

Our Technology

Smart-Array Card

Uses DNAMicroarray

Technology A DNA

Microarray/DNAchip is a piece ofglass or plastic on

which pieces of DNAhave been affixed ina microscopic array

8/7/2019 Multiple Microarray diagnostics for STDs main

8/17

8/7/2019 Multiple Microarray diagnostics for STDs main

9/17

8/7/2019 Multiple Microarray diagnostics for STDs main

10/17

Product Specification

Sensitivity andspecificity is for HIV,

gonorrhea, Chlamydia,syphilis, herpes

simplex virus 1&2.

Sub-Saharan African(16-60 yrs) starting with

Ethiopia

Reading time will be15mins

Shelf life is 24 months

Designatedpharmaceutical and

chemist outlets,hospitals, Universities

Urine samples 2-3drops

Card stored underroom temperature (4-

35c)

Cost is $5 for a packcontaining 3 Smart-

Array cards

8/7/2019 Multiple Microarray diagnostics for STDs main

11/17

Merits and Demerits

Merits

High specificity andsensitivity.

Faster results

Can compare the activityof many genes in diseased

and healthy cells

Can categorize diseasesinto stages

Demerits

Too much data all at once.

Can take quite a while to

analyze all the results.

The results may be toocomplex to interpret

The results are not alwaysreproducible

8/7/2019 Multiple Microarray diagnostics for STDs main

12/17

Target Market & Drive

Inexpensive

Sensitive

Equipment free

Rapid

Affordable

Accurate

Non-invasive

Easily accessible

8/7/2019 Multiple Microarray diagnostics for STDs main

13/17

Business EnvironmentAcceptability

Rapid test for 5 STDs ofdifferent strains

Ease of use and portability

Cost efficient

New technology

Joint ventures

Govt. collaboration

Bargaining Power ofCustomers

Acceptability

S.W.O.T

8/7/2019 Multiple Microarray diagnostics for STDs main

14/17

Market Leaders

Image from: Bwill, 2010

8/7/2019 Multiple Microarray diagnostics for STDs main

15/17

Future Prospects

Image from: Gnatchr, 2009

8/7/2019 Multiple Microarray diagnostics for STDs main

16/17

Conclusion

DNA based multiple disease

diagnosis can be exploited forSTI Prevention and Control

8/7/2019 Multiple Microarray diagnostics for STDs main

17/17

Curled from: vleindia.blogspot.com