MUC LUC. .

Ldi noi dftu fl

Chlidng 1

HOA CHATvA DUNG DJCH

I. Khai niem vg hoa chat r""'1. . .,II. Dung dich ",III. N6ng dQdung dich ~.'

IV. Fha dung dich tieu chu:1n d~ chu:1n dQ :

v. Cachtinh h~sodiguchinh :

VI. Bai t:%p ,...............................................

Chlidng 2

PHUdNG PHAp LAY MAU PHAN TicH

I. Lay mau.........................................................................................................................................

II. Chu:1n bi mau phan tich.........................................................................................................

III. Codinh mau 4.

Chlidng 3PHUdNG PHAp SO MAu

I. Phudng phap so mau """"""""""""""

II. Dinh lu:%t Lambert-Beer...........................................................................................................

III. Mau dung dich va chqn buoc song anh sang (hay chqn kinh lqc ffifw) l-.Chlidng 4. ~ ~

PREP DO QUANG PRO KE

I. Hap th\l ti'1 ngo~i cua cac lo~i cuvet khac nhau.................................................................

II. Quang ph6 hap th\l ti'1 ngo~i cua NAD+ va NADH.........................................................

III. Doc tinh khoi luQng NADH...................................................................................................

137

Chztdng 5

D~NH LU'(jNG HYDRAT CACBON 35

I. Dinh luQng dudng khli theo phudng philp Bertrand 35

II. Dinh luQng dudng khli theo phudng philp vi luQng cua Rodzevich, V.Y 38

III. Dinh luQng glucoza trong mati bling phudng philp Nelson 40

IV. Dinh'luQng fructoza trong dung dich colan dudng khli khac 41

V. Dinh luQng dudng khli bling phU:dng philp axit dinitrosalicylic 42

VI. Dinh luQng sacaroza theo phudng philp thuy ph an bling axit 43

VII. Dinh luQng tinh bQt theo phudng philp thuy phan bling axit 44

VIII. Dinh luQng xenluloza 46

IX. Dinh luQng pectin bling phudng philp canxi pectat 47

X. Dinh luQng dextrin bling phudng philp ket tua voi c6n 48

Chztdng 6

D~NH LU'(jNG LIPIT 50

I. Dinh luQnglipit bling may Soxhlet : 50

II. Xac dinh cae chi s5 cua lipit 53

Chztdng 7

D~NH LU'(jNG PROTEIN 58

I. Dinh luQng nitd bling phudng philp Kjedahl 58

II. Dinh luQng protein bling phudng philp Lowry 62

III. Dinh luQng protein bling Coomassie Brilliant Blue G. 250 63

IV. Dinh luQng protein bling phudng philp quang ph6 67

Chztdng 8, A? A A'

XAC D~NH HO~T DQ CUA MQT SO ENZYM 69

I. Dinh nghla ddn vi ho:;tt dQenzym 69

II. Chli Y khi xac dinh ho:;tt dQ enzym 69

III. Xac dinh ho:;tt dQ proteinaza (EC 3.4".) theo phudng philp Anson cai tien 70

IV. Xac dinh ho:;tt dQpepsin bling phudng, philp Anson 72V. Xac dinh ho:;tt dQAscorbat oxidaza 74

VI. Xac dinh ho:;ttdQpapain """"""""""""""""""""""""""" 75

VII. Xac dinh ho:;ttdQcua a-amylaza , 78

VIII. Xac dinh ho:;ttdQglucoamylaza 80

Ix. Xac dinh ho:;ttdQcatalaza 82

138

X. Xac difih ho~t dQlipaza 83

XI. Xac difih ho~t dQ peroxidaza 84

Chlidng 9

DJNH LU'<jNG AXIT NUCLEIC 87

I. Phudng phap Schmidt va Thannhauser 87

II. Phudng phap Schneider """"""""""""""""""""""""""" 89

III. Phudng phap Ogur va Rosen 89

IV. Phudng phap quang ph6 90

V. Difih luQnghQpchat phospho trong mo ruQt theo phudng phap Schmidtva Thannhauser co slia d6i 92

VI. Difih luQngphospho theo phudng phap Horecker va cae cQngs1;i 93

VII. Difih luQng phospho vo cd co ngu6n goc tU phospholipit theo phudngphap Delory 94

VIII. Difih luQngARN b&ngorxinol 95

IX. Difih luQngADN b&ngphudng phap diphenylamin 96

Chlidng 10

DJNH LU'<jNG VITAMIN 98

I. Difih luQng vitamin C b&ng phudng phap chuffn dQ 98

II. Difih luQng vitamin B2 b&ng phudng phap huynh quang 100

III. Difih luQng vitamin B1 b&ng phudng phap huynh quang 105

Chlidng 11

DJNH LU'<jNG MQT SO NGUYEN TO 107

I. Difih luQng phospho , 107

II. Dtnh luQng kali t6ng so cua th1;icv~t b&ngnatri cobantinitrit 112

III. Difih luQng canxi va magie t6ng so trong diy b&ngTrilon B 112

IV. Difih luQng canxi trong mo cd b&ngphudng phap Retinxki 114

V. Difih luQng s&t 115

Chlidng 12

PHV LVC 117

I. Cae dung dtch d~m 117

II. Dung dtch pH chuffn 122

III. N6ng dQaxit va amoniac thudng g(tp 123

139

IV. Fha dung dtch ph:ln tram axit va amanic 123

V. Phan tit hiQngva ty tr9ng cua mQtso axit 124

VI. Ki~m tra n6ng dQcac dung dtch chuftn dQda pha b&ngdung dtchchat goc co n6ng dQchinh xac 124

VII. Chi tht mati axit-bazd 125

VIII. Cach pha va sit dvng mQtso thuoc thit chi tht mati thong thudng 125

IX. Cac dung dtch rita dvng cv bftn trang phong thi nghi~m 127

X. Tr9ng luting nguyen tit cua mQtso nguyen to 127

XI. N6ng dQdung dtch amani sulfat baa hoa d nhi~t dQkhac nhau 127

XII. Cach tinh h;icly tam 128

XIII. Cac ky hi~u quy dtnh kich thuoc va cac ph:ln th~p phan 128

XIV. Cac chfl cai Hy L~p 128

xv. Cac tinh chat cua mQtso d6ng vt phong x~ ling dvng trang y h9C 129

Thu~t ngfl Anh-Vi~t 130

Tai li~u thalli khaa 135