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PhytoTechnology Laboratories® P.O. Box 12205; Shawnee Mission, KS 66282-2205 Phone: 1-888-749-8682 or 1-913-341-5343; Fax: 1-888-449-8682 or 1-913-341-5442 Web Site: www.phytotechlab.com © 2010 PhytoTechnology Laboratories® M5642-Info Page 1 of 1 PhytoTechnology Laboratories® Helping to Build a Better Tomorrow through Plant Science™ Product Information Sheet M5642 Murashige & Skoog (MS) Basal Medium, Van der Salm Modification Properties Form: Fine Powder Appearance: Light Burgandy Powder Application: Plant Tissue Culture Solubility: Water Typical Working Concentration: 4.46 g/L Storage Temp: 2-6° Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form. Other Notes: Contains the macro- & micronutrients and vitamins as described by Murashige and Skoog (1962) with the iron source as described by Van der Salm et al. (1994). Unadjusted pH = Approx. 3.5-4.5 Formula (mg/L) Ammonium Nitrate 1650 Boric Acid 6.2 Calcium Chloride, Anhydrous 332.2 Cobalt Chloride•6H 2 O 0.025 Cupric Sulfate•5H 2 O 0.025 FeNa-EDDHA 96.0 Magnesium Sulfate, Anhydrous 180.7 Manganese Sulfate•H 2 O 16.9 Molybdic Acid (Sodium Salt)• 2H 2 O 0.25 Potassium Iodide 0.83 Potassium Nitrate 1900 Potassium Phosphate, Monobasic 170 Zinc Sulfate•7H 2 O 8.6 Glycine (Free Base) 2 myo-Inositol 100 Nicotinic Acid (Free Acid) 0.5 Pyridoxine•HCl 0.5 Thiamine•HCl 0.1 Application Notes Plant Tissue Culture Tested Plant species: Originally published for use on the rose rootstock cv ‘Moneyway’ due to chlorosis in shoots cultured on the standard MS formula. References Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497. Van der Salm, TMP, CJG Van der Toorn, CH Hänisch ten Cate, LAM Dubois, DP De Vries, and HJM Dons. 1994. Importance of the iron chelate formula for micropropagation of Rosa Hybrida L. ‘Moneyway’. Plant Cell Tiss. and Organ Cult. 37:73-77. Revised 3/2010

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PhytoTechnology Laboratories® P.O. Box 12205; Shawnee Mission, KS 66282-2205

M5642-Info Page 1 of 1

PhytoTechnology Laboratories®Helping to Build a Better Tomorrow through Plant Science™

Product Information Sheet

M5642

Murashige & Skoog (MS) Basal Medium,Van der Salm Modification

Properties Form: Fine Powder

Appearance: Light Burgandy Powder Application: Plant Tissue Culture

Solubility: Water Typical Working

Concentration: 4.46 g/L

Storage Temp: 2-6° Storage Temp of

Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.

Other Notes: Contains the macro- & micronutrients and vitamins as described by Murashige and Skoog (1962) with the iron source as described by Van der Salm et al. (1994). Unadjusted pH = Approx. 3.5-4.5

Formula (mg/L) Ammonium Nitrate 1650

Boric Acid 6.2 Calcium Chloride, Anhydrous 332.2

Cobalt Chloride•6H2O 0.025 Cupric Sulfate•5H2O 0.025

FeNa-EDDHA 96.0 Magnesium Sulfate, Anhydrous 180.7

Manganese Sulfate•H2O 16.9 Molybdic Acid (Sodium Salt)• 2H2O 0.25

Potassium Iodide 0.83 Potassium Nitrate 1900

Potassium Phosphate, Monobasic 170 Zinc Sulfate•7H2O 8.6

Glycine (Free Base) 2 myo-Inositol 100

Nicotinic Acid (Free Acid) 0.5 Pyridoxine•HCl 0.5 Thiamine•HCl 0.1

Application Notes Plant Tissue Culture Tested Plant species: Originally published for use on the rose rootstock cv ‘Moneyway’ due to chlorosis in shoots cultured on the standard MS formula. References Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497. Van der Salm, TMP, CJG Van der Toorn, CH Hänisch ten Cate, LAM Dubois, DP De Vries, and HJM Dons. 1994. Importance of the iron chelate formula for micropropagation of Rosa Hybrida L. ‘Moneyway’. Plant Cell Tiss. and Organ Cult. 37:73-77.

Revised 3/2010

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