MOLECULAR GENETICS

Majury ArumaiPuneet KaurMandy SoRobert Wallis

OVERALL EXPECTATIONS

C1. Analyse some of the social, ethical, and legal issues associated with genetic research and biotechnology

C2. Investigate, through laboratory activities, the structures of cell components and their roles in processes that occur within the cell

C3. Demonstrate an understanding of concepts related to molecular genetics, and how genetic modifications is applied in industry and agriculture

D1.1 analyse on the basis of research, some of the social, ethical, and legal implications of biotechnology

D1.2 analyse, on the basis of research, some key aspects o Canadian regulations regarding biotechnology

D2.1 use appropriate terminology related to molecular genetics

D2.2 analyse simulated strand of DNA to determine the genetic code and base pairing of DNA

D2.3 conduct an investigation to extract DNA from a plant or animal specimen

D2.4 investigate and analyse the cell components involved in protein synthesis using appropriate lab equipment and techniques/computer simulation

D3.1 explain the current model of DNA replication, and describe the different repair mechanisms that can correct mistakes in DNA sequencing

D3.1 explain the current model of DNA replication, and describe the different repair mechanisms that can correct mistakes in DNA sequencing

D3.2 compare the structures and functions of RNA and DNA, and explain their roles in protein synthesis

D3.2 compare the structures and functions of RNA and DNA, and explain their roles in protein synthesis

D3.3 explain the steps involved in protein synthesis and how genetic expression is controlled in prokaryotes and eukaryotes by regulatory proteins

D3.3 explain the steps involved in protein synthesis and how genetic expression is controlled in prokaryotes and eukaryotes by regulatory proteins

D3.4 explain how mutagens can cause mutations by changing the genetic material in cells.

D3.4 explain how mutagens can cause mutations by changing the genetic material in cells.

D3.5 describe examples of genetic modification, and explain how it is applied in industry and agriculture

D3.5 describe examples of genetic modification, and explain how it is applied in industry and agriculture

D3.6 describe the functions of some of the cell components used in biotechnologyD3.6 describe the functions of some of the cell components used in biotechnology

D3.7 describe, on the basis of research, some historical scientific contributions that have advanced our understanding of molecular genetics

D3.7 describe, on the basis of research, some historical scientific contributions that have advanced our understanding of molecular genetics

1DNA structure

2DNA structureHistory of DNA

3History of DNA

4DNA extraction lab

5DNA replication

6DNA replication

7 QUIZPCR

8Transcription

9Translation

10Post-translational modifications

11Catch up day

12 QUIZLac and Trp operons

13Mutations

14Gene Organization

15PlasmidsRestriction enzymes

16GMOs

17VNTR/SNP/STR

18Electrophoresis

19Cloning

20Microarrays

21Review

22 Unit Test

• A1.1A1.1 – formulate hypotheses to focus inquiries or research

• A1.5A1.5 – conduct inquiries using appropriate materials and equipment to collect observations

• A1.8A1.8 – confirm/refute hypotheses• A1.10A1.10 – draw conclusions based on inquiry results• A1.11A1.11 – communicate plans, procedures & results• D2.3D2.3 – conduct an investigation to extract DNA

from a specimen of plant or animal

What do we need?

BANANA

STRAINER

CONTACTS SOLUTION

RUBBING ALCOHOLDETERGENT

SALTPESTLE & MORTAR

STEP 1:BREAK THE CELL WALL

STEP 2: GET RID OF SOLIDS

STEP 3:BREAK THE MEMBRANES

STEP 4:

PROTEASES

PROTEIN BREAKDOWN

STEP 5:

70-90%

PRECIPITATE THE DNA

Lab Groups

SusanMerylSallyTyler

LauraAmy P

Chris WongAlessia

PaulJane

MelanieChris Walsh

SheenaKarenPhilip

KatarinaRuthGabeJenny

BradAlicia

Amy Z.

11 22 33

44 55 66

DNA

RNA

Protein

transcription

translation

replication

A C

G TDNA

RNA

A C

G

GTDNA

AC G CT

mRNAG AA

tRNA

C

Glu

tRNA AA Letter

AAA Phe FAAC Leu LAAG Phe FAAU Leu LACA Cys CACC Trp WACG Cys CACU Thr OAGA Ser SAGC Ser SAGG Ser SAGU Ser SAUA Tyr YAUC spc -AUG Tyr YAUU spc -

tRNA AA Letter

CAA Val VCAC Val VCAG Val VCAU Val VCCA Gly GCCC Gly GCCG Gly GCCU Gly GCGA Ala ACGC Ala ACGG Ala ACGU Ala ACUA Asp DCUC Glu ECUG Asp DCUU Glu E

tRNA AA Letter

GAA Leu LGAC Leu LGAG Leu LGAU Leu LGCA Arg RGCC Arg RGCG Arg RGCU Arg RGGA Pro PGGC Pro PGGG Pro PGGU Pro PGUA His HGUC Glu QGUG His HGUU Glu Q

tRNA AA Letter

UAA Iso IUAC Met MUAG Iso IUAU Iso IUCA Ser SUCC Arg RUCG Ser SUCU Arg RUGA Thr TUGC Thr TUGG Thr TUGU Thr TUUA Asn NUUC Lys KUUG Asn NUUU Lys K

tRNA

C

Glu

Letters of the alphabet

E

1) THE CENTRAL DOGMA IS ON THE 1) THE CENTRAL DOGMA IS ON THE TESTTEST

2) PHIL DO WE HAVE THE TWENTY 2) PHIL DO WE HAVE THE TWENTY FIFTH OFFFIFTH OFF

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