molecular basis of inheritance€¦ · in prokaryotes, the predominant site (or primary step) of...
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Molecular Basis
Of Inheritance
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In prokaryotes, the predominant site (or primary
step) of control of gene expression is at
(A) Control of rate of transcriptional initiation.
(B) Control of rate of translational initiation.
(C) Control of rate of initiation of replication
(D) Processing level.
01
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Which of the following events would occur in 'Lacoperon of
E. coli when growth medium has high concetration of lactose
(A) Structural genes fail to produce polycistronic mRNA
(B) Repressor protein binds to RNA polymerase and
prevents translation
(C) Repressor protein attaches to the promoter sequence and
represses the operator
(D) Inducer molecule binds to repressor protein and RNA
polymerase binds to promoter sequence.
02
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Environmental agent triggering transcription
from an operon is
(A) Inducer
(B) Regulator
(C) Repressor
(D) Controlling factor.
03
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Genes regulate growth and differentiation
through
(A) Transcription and translation
(B) Transduction and translation
(C) Transformation
(D) Translation.
04
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In operon model, regulator gene functions as
(A) Repressor
(B) Regulator
(C) Inhibitor
(D) All of the above.
05
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Operon contains
(A) Operator and regulator genes
(B) Operator and structural genes
(C) Operator and regulator genes, and repressor
(D) Operator gene, regulator gene, repressor ,
structural genes and promoter gene.
06
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In Escherichia coli, the product of i gene combines
with
(A) Operator gene to switch off structural genes
(B) Inducer gene to switch off structural genes
(C) Opertor gene to swhitch on structural genes
(D) Regulator gene to switch off structural genes.
07
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Lac operon concept was proposed by
(A) Jacob and Monod in 1961
(B) Jacob and Monod in 1969
(C) Watson and Crick in 1953
(D) Watson and Crick in 1963.
08
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Operon model of gene regulation and
organisation of procaryctes was proposed by
(A) Messelson and Franklin
(B) Wilkins and Franklin
(C) Beadle and Tatum
(D) Jacob and Monod.
09
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What is correct gene expression pathway
(A) Gene – mRNA
(B) Transcription– gene – translation – mRNA –
protein
(C) Gene – transcription – mRNA – translation –
protein
(D) Gene – translation – mRNA – transcription –
protein
10
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Choose the wrong statement regarding the
observation drawn from Human Genome Project is
(A) Repetitive sequences are stretches of RNA
(B) Less than 2% of the genome codes for protein
(C) Chromosome 'Y' has the fewest number of genes
(D) SNPs help in tracing human history.
11
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Sequencing the whole set of genome that contained
all the coding and non coding sequence and later
assigning different region in the sequence with
functions are referred to as
(A) Expressed Tags
(B) Sequence Annotation
(C) ESTs
(D) SNPs.
12
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Coenorhabditis elegans is
(A) Free-living
(B) Non-pathogenic
(C) Nematode
(D) All of the above.
13
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Many non-humans model organisms have also been
sequenced along with the human genome, these are
(A) Bacteria and yeast
(B) Plants (rice and Arabidopsis)
(C) Fruitfly and Coenohabditis (nematode)
(D) All of the above
14
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The application of genetic engineering is in the field
of
(A) Forensic science
(B) Genetic biodiversity
(C) Evolutionary biology
(D) All of the above.
15
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Most number of genes are located on chromosome
(A) 1
(B) 6
(C) X
(D) Y.
16
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Automated–DNA sequencers is based or method
developed by
(A) Erwin Chargaff
(B) Maurice Wilkins
(C) Francis Crick
(D) Frederick Sanger.
17
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Single base DNA differences are called
(A) VNTR
(B) SCP
(C) SNPs
(D) Expressed sequence tags.
18
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Largest gene in humans is
(A) Dystrophin
(B) Insulin gene
(C) Oncogene
(D) Huntingtin.
19
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Haploid content of human DNA is
(A) 1.65 × 109 bp
(B) 3.1 – 3.3 × 109 bp
(C) 4.6 × 106 bp
(D) 3.3 × 109 kbp.
20
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Select the correct sequences of steps in DNA fingerprinting involving
Southern blot hybridisation using radiolabelled VNTR as proble
I. Hybridisation using labelled VNTR probe.
II . Isolation of DNA
III. Transferring (blotting) of separated DNA fragments to synthetic
membranes, such as nitrocellulose or nylon.
IV. Detection of hybridisation DNA fragments by autoradiography.
V. Separation of DNA fragments by electrophoresis.
VI . Digestion of DNA by restriction endonucleases
(A) I, V, VI, II, III and IV (B) II, VI, V, III, I and IV
(C) V, I, VI, III, IV and II (D) II, I, V, VI, IV and III.
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Find the wrongly matched pair
(A) Hargobind Khorana-Synthesised RNA molecules
chemically
(B) George Gamow-Codon in triplet
(C) Meselson and Stahl-Regulation of gene
expression
(D) Alec Jeffreys-DNA fingerprinting.
22
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If an inheritable mutation is observed in a
population at high frequency, it is referred as
(A) DNA polymorphism
(B) Expressed sequence tag
(C) Sequence annonation
(D) Linkage.
23
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The VNTR belongs to a class of satellite DNA
reffered to as
(A) Repetitive DNA
(B) Mega-Satellite
(C) Mini-Satellite
(D) Micro-Satellite.
24
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Which is incorrect about DNA polymorphism
(A) The two alleles of a chromosome contain different
copy numbers of VNTR
(B) Different chromosomes contains different copy
number of VNTR
(C) Polymorphisms are inheritable from parents to
children
(D) DNA from different tissue from an individual shows
the different degree of polymorphism.
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Matching DNA sequence of criminal with suspect
is known as
(A) DNA fingerprinting
(B) DNA amplification
(C) Gene mapping
(D) DNA resolution
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One of the following is major requirement for
DNA fingerprinting
(A) Electron microscopy
(B) Electrophoresis
(C) ELISA
(D) HPLC
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Transfer of DNA bands from agarosegel to
nitrocellulose or nylon membrane is
(A) Southern transfer
(B) Western transfer
(C) Northern transfer
(D) Eastern transfer
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Which cannot be used for DNA fingerprinting in
humans
(A) Leucocytes
(B) Erythrocytes/RBCs
(C) Hair bulbs
(D) Sperms.
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Which is an example of highly repetitive DNA
(A) Alu elements
(B) Histone gene cluster
(C) Dispersed DNA
(D) DNA minisatellite.
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Blood stain component to be used for DNA
profiling technique is
(A) Serum
(B) Leucocytes
(C) Platelets
(D) Erythrocytes.
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DNA fingerprinting technique was developed by
(A) Jeffreys et al
(B) Schleiden and Schwann
(C) Boysen and Jensen
(D) Edwards and Steptoe
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The probe used initially by Alec Jaffrey during
development of DNA fingerprinting was
(A) Ribozyme
(B) SAT chromosomes
(C) VNTR
(D) rDNA.
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DNA fingerprinting in forensic science was
pioneered in India by
(A) Lalji Singh
(B) H.G. Khorana
(C) J.C. Bose
(D) M.S. Swaminathan.
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Which is useful in solving parental disputes
(A) Hybridoma technology
(B) DNA Fingerprinting
(C) Western blotting
(D) ELISA.
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