mmb1 lec2 qb2013
DESCRIPTION
TRANSCRIPT
ACTIVITIES
DATE ACTIVITY
JULY 5 LAST PPT LECTURE
JULY 9 VIRUTAL LABORATORY ACTIVITY
JULY 12 FIRST EXAMINATION
JULY 16 FREE DAY: LIBRARY WORK ON HOW TO DEVELOP YOUR GMO
JULY 19-23 GROUP PRESENTATION (PPT) -What to Develop -Why Develop -How will you Develop -Dangers or Issues to Expect
JULY 26 APPLICATION OF BIOTECHNOLOGY LECTURE SERIES
REQUIREMENTS
• GMO DEVELOPMENT PRESENTATION
• BIOWEEK EXHIBIT
• FIELD TRIP PORTFOLIO • Expectations Before the Trip • Learnings During the Trip • Advocacy: Spreading knowledge of
Biotechnology to Others
exams
• Examination 1: INTRODUCTION TO TECHNIQUES IN BIOTECHNOLOGY
• Examination 2: APPLICATIONS OF BIOTECHNOLOGY
• Examination 3: LAWS AND INTERNATIONAL GUIDELINES CONCERNING BIOTECHNOLOGY
• Take-home (online)
LAST MEETING: HOW WERE YOUR GENES ISOLATED?
• Golden Rice
• BT Corn
• Edible Vaccine in Potato
• GMO Soya
• Recombinant Hepatitis B vaccine
www.designingdigitally.com
GENE ISOLATION
www.koihealth.org
Source of Genes Primers will amplify target Gene of Interest
Amplicon for “CONSTRUCT”
www.invitrogen.com
www.sciencedirect.com
POLYMERASE CHAIN REACTION
www.neb.com
www.teachersdomain.org
MAKING YOUR CONSTRUCT
www.addgene.org
EXAMPLES OF RESTRICTION ENZYMES
TRANSFORMATION & SELECTION MARKERS
bio.davidson.edu passel.unl.edu
BACTERIAL CLONING: THE PROCESS
CONFIRMATION OF EXPRESSION
• HOW WILL YOU KNOW IF THE GENE THAT YOU INSERTED IS EXPRESSED?
viswagenbiotech.com en.wikipedia.org igece.org
www.designingdigitally.com
HOW ABOUT PLANTS?
A plant part Is cultured
Callus grows
Shoots develop Shoots are rooted;
plant grows to maturity
clonal-solutions.com.au
DELIVERING GENES (Agrobacterium & The Gene Gun)
apps.rhs.org.uk users.comcen.com.au
ehow.com weirdasianews.com cbc.ca
Cloning overview
• Organismal cloning produces one or more organisms genetically identical to the “parent” that donated the single cell
• may lead to production of stem cells for research and other applications
• CLONE: genetically identical individuals produced from one parent
toonrefugee.com
Cloning plants
bbc.co.uk
• A totipotent cell is one that can generate a complete new organism
• Plant cloning is used extensively in agriculture
Cloning animals
• Nuclear Transfer (e.g. BRANDY (2004) the cow and DOLLY (1997) the sheep) • the nucleus of an unfertilized egg cell or zygote
is replaced with the nucleus of a differentiated cell
• Embryo Splitting (production of high value herds and livestock breeds)
Nuclear transfer
skinnerscience.com
Embryo splitting
csus.edu
HUMAN CLONING & STEM CELLS
Should we or Should we NOT?
2 kinds of cloning
ipscell.com
The ivf way to have a baby
Stem cell therapy stemcellsforhope.com
Stem cells for therapy
home.honolulu.hawaii.edu
READING ASSIGNMENT: Success stories and fail stories using
stem cell therapy Be sure to Identify the points of success and points of failure
thinkprogress.org
NEXT MEETING: the virtual laboratory
PER GROUP: 1. Laptop
2. Internet Source
ACTIVITY THE VIRTUAL LABORATORY
Main reference: http://learn.genetics.utah.edu
WE WILL NEED AN I-NET CONNECTION
• http://learn.genetics.utah.edu/content/labs/extraction/
• http://learn.genetics.utah.edu/content/labs/pcr/
• http://learn.genetics.utah.edu/content/labs/gel/
• http://learn.genetics.utah.edu/content/labs/microarray/
• http://learn.genetics.utah.edu/content/tech/cloning/clickandclone/
FOR DISCUSSION • What is the significance of DNA isolation?
• When is a PCR useful?
• Why do bands appear in an electrophoresis?
• How can a microarray data reveal gene expression?
• When do you think cloning is not indicated?
NEXT MEETING: EXAM exam number 1