mission impossible ii

The

mission ImpossibleII

JSTP 12

The

mission

Impossible II

The toy story

Impossible

Themission

ImpossibleProject

โครง

โครงโครงงาน

โครงโครงงานProject

PROJECT = TOYGAME

FUN

แบบฟอรมโครงงาน ทมาและความสำคญ วตถประสงค

ประโยชนทคาดวาจะไดรบ ตวแปรทเกยวของ

คมอการเลน

เลนอยางไรใหส

นก

คมอการเลน

เลนอยางไรใหส

นก

อานจบแลว จะสนกไหม

Every years someone has a great speech....

@#!$#%wee-tah-kah loo-loo

คนหจงบองตง

ตอยอด

ตอยอด

สรางยาลดอาการ คนหของFurby

Innovation

What did we play ?

Detective

What did we play ?

Detective Created

What did we play ?

Detective Created Divas

What did we play ?


Action

What did we play ?


Business

Action

What did we play ?


Impersonate

Business

Action

What did we play ?


Impersonate

Business

Action

What did we play ?

ซอนแอบ ไขปรศนา


Impersonate

Business

Action

What did we play ?


เลน Lego พบจรวด หนยนต


Impersonate

Business

Action

What did we play ?



รองเพลง แตงตว


Impersonate

Business

Action

What did we play ?




เลนเกม แปลงราง


Impersonate

Business

Action

What did we play ?




เกมเศรษฐ เลนขายของ



Impersonate

Business

Action

What did we play ?




บทบาทสมมต หมอรกษาคนไข

เกมเศรษฐ เลนขายของ


P.P. TOYs

Bacteria

BacteriaStyrofoam

Biodegradation

BacteriaStyrofoam

My project in academic style.

Styrofoam

Styrofoam

Styrene monomer

Polystyrene

Chemical formula is (C8H8)n

Monomer styrene

Thermoplastic

Blowing agents

Polystyrene

Chemical formula is (C8H8)n

Monomer styrene

Thermoplastic

Blowing agents

500 years++ for degradation

http://en.wikipedia.org/wiki/Polystyrene

Recycling & Incineration

Recycling & Incineration

1% turn back to polystyrene

most of the polystyrene is converted into carbon dioxide, water vapor, and heat.

"Ease of Disposal". Retrieved 2009-06-25

Photo by Napat Jaitui JSTP # 15

Styrene Monomer

Carbon Monoxide


Styrene Monomer

Carbon Monoxide


CARCINOGEN

cc

c cc

cc c

cc

c cc

cc c

Bacterianutrition‣ Energy source‣ Carbon source‣ Nitrogen source‣ Minerals‣Water‣ Growth factors

How can I find

the bacteria that able to

degrade a styrofoam?

SOILLandfill

Landfill foam

How can I find

the bacteria that able to

degrade a styrofoam?

The ability

The key to isolate the effective microbe is the

degradability.

MSM Broth Bacteria selective broth

MSM broth‣K2HPO4

‣KH2PO4

‣(NH4)2SO4

‣MgSO4

‣ FeSO4.2HO2

‣MnCl2.4H2O‣CoCl2.6H2O‣CuCl2.2H2O‣NiCl2.6H2O‣Na2MoO4.2H2O‣ZnSO4.7H2O‣H3BO3

Sterile Styrofoam


Shake in shaker for 1 monththen inoculate to new freshbroth for sub culture.

Every week the solution in each flask was taken to the eppendorf then stored at 2C๐ for stop bacteria growth that use for monitor the changing of bacteria population.

MSM broth‣K2HPO4

‣KH2PO4

‣(NH4)2SO4

‣MgSO4

‣ FeSO4.2HO2

‣MnCl2.4H2O‣CoCl2.6H2O‣CuCl2.2H2O‣NiCl2.6H2O‣Na2MoO4.2H2O‣ZnSO4.7H2O‣H3BO3

Sterile Styrofoam



Foam in landfill Soil in landfill

WeekDominant

species

WeekDominant

speciesDiversity

Every week the solution in each flask was taken to the eppendorf then stored at 2C๐ for stop bacteria growth that use for monitor the changing of bacteria population.

DNA Replication : PCR (TopTaq Master Mix Kit)

16S rRNA gene Amplification by using Primer VR (Medlin et al., 1998) & VFC (Muyzer et al., 1993)

DGGE (Denature Gradient Gel Electrophoresis)

30% Urea + Formamine



- 30% Urea + Formamine


+




GC rich




GC low

GC rich

Mar

ker

Soil

wee

k 1

Soil

wee

k 5

Soil

wee

k 6

Soil

wee

k 7

Soil

wee

k 8

Soil

wee

k 20

Foam

wee

k 1

Foam

wee

k 5

Foam

wee

k 6

Foam

wee

k 7

Foam

wee

k 8

Foam

wee

k 20

Con

trol

wee

k 6

Con

trol

wee

k 7

Con

trol

wee

k 8

Mar

ker

Mar

ker

Neg

ativ

e

Bacteria from foam Bacteria from soil Control

DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl.


The microscopic structure of Polystyrene observed by SEM

Pat Pataranutaporn1, Apinya.J2 Assistant prof. Savaporn Supaphol3, 1 PSU.Wittayanusorn school, 2Scientific Equipment Center

Prince of Songkla University 3Kasetsart University

Control : Polystyrene in MSM broth without bacterial source.

Regular polystyrene foam that didn’t use in experiment.

100x 200x 500x

The comparison of Polystyrene microscopic structure

Control

Regular polystyrene that didn’t use in experiment.

Control

Polystyrene in Medium with bacteria from foam sample.

100x 200x 500x


Regular polystyrene foam that didn’t use in experiment.Polystyrene in Medium with bacteria from foam sample.

Bacteria from foam

Polystyrene in Medium with bacteria from foam sample.

100x 200x 500x



Bacteria from foam

Polystyrene in Medium with bacteria from soil sample.

100x 200x 500x



Bacteria from soil

Polystyrene in Medium with bacteria from soil sample.

100x 200x 500x


Regular polystyrene foam that didn’t use in experiment.Polystyrene in Medium with bacteria from soil sample.

Bacteria from soil

Soil

wee

k 1

Soil

wee

k 5

Soil

wee

k 6

Soil

wee

k 7

Soil

wee

k 8

Foam

wee

k 1

Foam

wee

k 5

Foam

wee

k 6

Foam

wee

k 7

Foam

wee

k 8

Con

trol

wee

k 6

Con

trol

wee

k 7

Con

trol

wee

k 8

Foam sampleSoil sampleControl

Soil

wee

k 1

Soil

wee

k 5

Soil

wee

k 6

Soil

wee

k 7

Soil

wee

k 8

Foam

wee

k 1

Foam

wee

k 5

Foam

wee

k 6

Foam

wee

k 7

Foam

wee

k 8

Con

trol

wee

k 6

Con

trol

wee

k 7

Con

trol

wee

k 8

Soil

wee

k 1

Soil

wee

k 5

Soil

wee

k 6

Soil

wee

k 7

Soil

wee

k 8

Foam

wee

k 1

Foam

wee

k 5

Foam

wee

k 6

Foam

wee

k 7

Foam

wee

k 8

Con

trol

wee

k 6

Con

trol

wee

k 7

Con

trol

wee

k 8

Foam sampleSoil sampleControl

Molecular cloning DNA isolation & DNA Analyzing

Pat Pataranutaporn1, Sureeporn Nualkaew2, Prof. Dr. Amornrat Phongdara2 1 PSU.Wittayanusorn school, 2 Prince of Songkla University

Mar

ker

Soil

wee

k 1

Soil

wee

k 5

Soil

wee

k 6

Soil

wee

k 7

Soil

wee

k 8

Soil

wee

k 20

Foam

wee

k 1

Foam

wee

k 5

Foam

wee

k 6

Foam

wee

k 7

Foam

wee

k 8

Foam

wee

k 20

Con

trol

wee

k 6

Con

trol

wee

k 7

Con

trol

wee

k 8

Mar

ker

Mar

ker

Neg

ativ

e




Mar

ker

Soil

wee

k 1

Soil

wee

k 5

Soil

wee

k 6

Soil

wee

k 7

Soil

wee

k 8

Soil

wee

k 20

Foam

wee

k 1

Foam

wee

k 5

Foam

wee

k 6

Foam

wee

k 7

Foam

wee

k 8

Foam

wee

k 20

Con

trol

wee

k 6

Con

trol

wee

k 7

Con

trol

wee

k 8

Mar

ker

Mar

ker

Neg

ativ

e




Hello Who are you ?

Molecular cloning Despicable Me: Minion Mayhem

PCR Product

PCR Product

Mix DNA for ligation

pGEM- T easy

Target DNA

PCR Product

Mix DNA for ligation

Transform plasmid to the competent cell.

E.Coli as



The separate DNA

Mar

ker

Soil

wee

k 1

Soil

wee

k 5

Soil

wee

k 6

Soil

wee

k 7

Soil

wee

k 8

Soil

wee

k 20

Foam

wee

k 1

Foam

wee

k 5

Foam

wee

k 6

Foam

wee

k 7

Foam

wee

k 8

Foam

wee

k 20

Con

trol

wee

k 6

Con

trol

wee

k 7

Con

trol

wee

k 8

Mar

ker

Mar

ker

Neg

ativ

e




ElectropherogramCode name : Control 4

Code name : Control 4

Basic Local Alignment Search Tool (BLAST)

Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea Description 16S ribosomal RNA sequences (Bacteria and Archaea) Program BLASTN 2.2.27+ Molecule type nucleic acid Query Length 1055

Code name : Control 4Phylogenetic tree

ElectropherogramCode name : Control 7

Code name : Control 7

Basic Local Alignment Search Tool (BLAST)

Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea Description 16S ribosomal RNA sequences (Bacteria and Archaea) Program BLASTN 2.2.27+ Molecule type nucleic acid Query Length 1006

Code name : Control 7Phylogenetic tree

Code name : Fe5

Bacterial plating Plate streaking & Plate spreading

Pat Pataranutaporn1, Panwong Kuntanawat2 1 PSU.Wittayanusorn school, 2 King Mongkut's University of Technology Thonburi

Bacterial plating on MSM & MSM + PSA agar cultured at 37oC over 48 hr.


Polystyrene-coacrylic acid (PSA)(particles diameter 500 nm)

MSM Agar


Polystyrene-coacrylic acid (PSA)(particles diameter 500 nm)

MSM Agar

Polysaccharide

Cx(H2O)y

Research Achievements

Research Achievements

13th NCSC, Jaipur India 2011

Youth summit 2012, Dubai UAE

JSTP Scholarship

STT 36

BYEE, Leverkuzen Germany

BYEE Poster Prize from india

Publication & media

JSTPMedia www.jstpmedia.org

Biodegradation of Polystyrene Foam by the Microorganisms

from Landfill

P.P. TOYs

หนงตะลง

หนงตะลงWEB app

หนงตะลงWEB appCulture

Developed in SWF format http://jstpmedia.org/images/complete.swf

A S ◀ ◀

◀

◀

move to the next character

Head(Speak)

Left hand right hand

Body flip

Demo

http://jstpmedia.exteen.com/images/complete.swf

JSTP 15/3

P.P. TOYs

SpaceGravity

Space

EnzymeGravity

The Evaluation of Amylase Activity under Microgravity

By JSTP 12 Pat Pataranutaporn Pondet Ananchai Arnan Schuett

ChemistryBiotechnology Electronic

Research Team

JSTP 12

ChemistryBiotechnology Electronic

Research Team

JSTP 12The youngest team ever!

Possibility of Enzyme


Collision Theory


Collision Theory‣Enzyme Concentration‣Substrate Concentration‣Inhibitors‣Temperature‣pH

Gravity

GravityFree motion of molecule

Salivary stress markers and psychological stress in simulated microgravity: 21 days in 6° head-down tilt.

Rai B, Kaur J. Catholic University Leuven, Belgium.

Spaceflight occurs in an environment of temperature extremes, microgravity, solar and galactic cosmic radiation, lack of atmospheric pressure, and high-speed micrometeorites. Exposure to microgravity and the space environment during space missions of short and long duration has important medical and health implications for astronauts. Psychological well-being is of increasing importance in planned spaceflights and interplanetary missions of long duration. The 6° head-down tilt (HDT) is an established method of mimicking low gravity on earth. The aim of the present study was to determine the effects of 21 days of HDT on psychological stress in 12 healthy male volunteers.

Psychological state was assessed by the current stress test, and chromogranin-A (CgA), cortisol, alpha-amylase, and beta-endorphin were measured in saliva. After one week of HDT, all volunteers developed psychological stress, and secretion of CgA, cortisol, alpha-amylase, and beta-endorphin were all significantly higher. Thus, 6° HDT appears to be a valid model to induce psychological stress changes in the immune system, changes that might also be encountered by astronauts and cosmonauts during both a short stay in space, such as that required while orbiting a space station, and in longer spaceflights.

Adaptation mechanism

http://www.ncbi.nlm.nih.gov/pubmed?term=Rai%20B%5BAuthor%5D&cauthor=true&cauthor_uid=21467821

http://www.ncbi.nlm.nih.gov/pubmed?term=Kaur%20J%5BAuthor%5D&cauthor=true&cauthor_uid=21467821

Difference enzyme activity rate affected by gravity

Enzyme containing

Enzyme containing

Pumping system

Enzyme containing

Pumping system

Starch measuring

Enzy

me

amyl

ase

Star

chA

cedi

c ac

id

Enzyme containing

Pumping system

Starch measuring

Enzy

me

amyl

ase

Star

chA

cedi

c ac

id

Enzyme containing

Pumping system

Starch measuring

2

Enzy

me

amyl

ase

Star

chA

cedi

c ac

id

Enzyme containing

Pumping system

Starch measuring

24 2

Enzy

me

amyl

ase

Star

chA

cedi

c ac

id

Enzyme containing

Pumping system

Starch measuring

246 2 24

Enzy

me

amyl

ase

Star

chA

cedi

c ac

id

Enzyme containing

Pumping system

Starch measuring

2468 2 2 24 46

Enzy

me

amyl

ase

Star

chA

cedi

c ac

id

Enzyme containing

Pumping system

Starch measuring

2468 2 2 2 04 46

Enzy

me

amyl

ase

Star

chA

cedi

c ac

id

Enzyme containing

Pumping system

Starch measuring

2468 2 2 2 04 46

low starch remain High starch remain

026 48

starch remain (%v/w)

026 48


Time (s) 026 48


Time (s) 026 48

Y


Time (s) 026 48

Y

X


Time (s) 026 48

Y

XRate =

Working period

Working period2 moths for discussing

Working period2 moths for discussing3 hours for proposal


1 night for presentation


1 night for presentation5 minutes for presentation rehearsing

JSTP TeamJSTP Team

ไมม limit ชวตเกนรอย

PP’s TOYP.P. TOYs

What you are seeing

now.

Presentation

DESCIPost modern

interface

Available at JSTPMedia

soon !

You have a chance to answer your own question .

ของเลนทสนก เราตองเลอกเอง

“ Never be afraid to asked “

POSSIBLEIM

AKE POSSIBLEI M

Right now...

It’s yourturn

THANK YOU

mission impossible ii

Science