min 00.250.50.7511.251.51.752 mau 0 25 50 75 100 125 150 175 mau min 00.250.50.7511.251.51.752 0 25...

of 7/7
min 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 mAU 0 25 50 75 100 125 150 175 mAU min 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 0 25 50 75 100 125 150 175 min 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 mAU 0 25 50 75 100 125 150 175 3.5 um 5 um 1 2 3 4 5 6 7 1 2 3 4 5 6 7 1 2 3 4 5 6 7 1 4-Acetamidophenol 2 Caffeine 3 2-Acetamidophenol 4 Acetanilide 5 Acetylsalicylic Acid 6 Phenacetin 7 Salicylic Acid LC Conditions Column: SB-C18, 4.6 x 30 mm Detector: 254 nm Injector: 1 ul, Mobile Phase: 1% Formic Acid Aceton (82:18 Flow: 2.0 ml/min 1.8 um Aumento della Risoluzione in funzione del Particle Size

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  • Slide 1
  • min 00.250.50.7511.251.51.752 mAU 0 25 50 75 100 125 150 175 mAU min 00.250.50.7511.251.51.752 0 25 50 75 100 125 150 175 min 00.250.50.7511.251.51.752 mAU 0 25 50 75 100 125 150 175 3.5 um 5 um 1 2 3 4 5 6 7 1 2 3 4 5 6 7 1 2 3 4 5 6 7 1 4-Acetamidophenol 2 Caffeine 3 2-Acetamidophenol 4 Acetanilide 5 Acetylsalicylic Acid 6 Phenacetin 7 Salicylic Acid LC Conditions Column: SB-C18, 4.6 x 30 mm Detector: 254 nm Injector: 1 ul, Mobile Phase: 1% Formic Acid Acetonitrile (82:18) Flow: 2.0 ml/min 1.8 um Aumento della Risoluzione in funzione del Particle Size
  • Slide 2
  • Meno particelle pi piccole Piccola quantit di particelle pi grandi
  • Slide 3
  • min 12345670 1.8m:Rs(4,5) = 5.45 Peak Width 5s(9) = 0.075 5m: Rs(4,5) = 3.63 Peak Width, 5s (9) = 0.103 Rs = +50% Aumento della Risoluzione in funzione del Particle Size + 50% Resolution (Theory: + 67%) + 125% Efficiency (Theory: + 177%) Colonna SB-C18 RRHT 4.6 x150mm Massima lunghezza
  • Slide 4
  • Rs(1,2) = 3.1 0.511.522.5 1 2 3 4. 4.6 x 250 mm, 5 m 1 mL/min min 051015202530 4.6 x 100 mm, 3.5 m 1 mL/min 4.6 x 30 mm, 1.8 m 1 mL/min 4.6 x 30 mm, 1.8 m 2 mL/min min051015202530 min051015202530 min 051015202530 29.65 12.71 4.15 2.09 1 2 3 1 1 2 2 3 3 4 4 4 Rs(1,2) = 4.8 Rs(1,2) = 3.5 Rs(1,2) = 3.3 N=22680 N=11691 N=6104 N=6568 N=6460 N=6463 N=21848 1100 HT + 1.8um RRHT : Reduction of analysis time from 30 min to 2 min Speed: 14x faster than 4.6 x 250mm, 5um Time saving: 92% Resolution: -35% AUMENTO DELLA VELOCITA DANALISI CON HPLC 1100
  • Slide 5
  • Speed Sample: 7 Analgesics Column: Zorbax RRHT SB-C18, 1.8 um, 4.6 x 30 mm Rs 2,1 = 2.1 Rs 2,1 = 2.2 Rs 7,6 = 2.2 Higher flow rate: higher speed same resolution higher sensitivity/precision 2 ml/min 90bar 3 ml/min 133bar 4 ml/min 181bar 2x faster than 2ml/min without loss in resolution! Ultra-fast analysis in 26 sec 4 peak width ~ 0.6 sec < 50 sec cycle time 7 analgesics separated in 21 sec for peaks 1,2,3 Total time Injection-to-Injection
  • Slide 6
  • HPLC, 40C SB-C18 4.6 x 150mm, 5m 1.20ml/min, 40C Analysis Time = 11min min 0246810 Rapid Resolution HT Columns Up to 20X Faster RRHT SB-C18 2.1mm x 50mm 1.8m 1.00ml/min, 40C Analysis Time= 1.1min RRLC, 40C 10x faster min 0.20.40.60.81 0 0.20.6 0 0.4 min RRHT SB-C18 2.1mm x 50mm 1.8m 2.40ml/min, 95C Analysis Time: 0.4min RRLC, 95C 27x faster >20X faster Up to 20x faster than conventional HPLC With same or better performance (resolution, precision, sensitivity, carry over) Compliant with strictest regulatory performance requirements
  • Slide 7
  • min 12345670 4 5 1.8m:Rs(4,5) = 5.45 Peak Width 5s(9) = 0.075 5m: Rs(4,5) = 3.63 Peak Width, 5s (9) = 0.103 Rs = +50% NON SOLO VELOCITA MA ANCHE EFFICIENZA + 50% Resolution (Theory: + 67%) + 125% Efficiency (Theory: + 177%) Colonna SB-C18 RRHT 4.6 x150mm Massima lunghezza