min0 0.25 0.5 0.75 1 1.25 1.5 1.75 2

mAU

0255075

100125150175

mAU

min0 0.25 0.5 0.75 1 1.25 1.5 1.75 20

25

50

75

100

125

150

175

min0 0.25 0.5 0.75 1 1.25 1.5 1.75 2

mAU

0

25

50

75

100

125

150

175

3.5 um

5 um

12

3

45 6

7

12

3

4

56

7

12

3

45

67

1 4-Acetamidophenol2 Caffeine3 2-Acetamidophenol4 Acetanilide5 Acetylsalicylic Acid6 Phenacetin7 Salicylic Acid

LC ConditionsColumn: SB-C18, 4.6 x 30 mmDetector: 254 nmInjector: 1 ul, Mobile Phase: 1% Formic Acid Acetonitrile (82:18)Flow: 2.0 ml/min

1.8 um

Aumento della Risoluzione in funzione del Particle Size

0,0

0,2

0,4

0,6

0,8

1,0

1,2

1,4

1,6

1,8

2,0

2,2

2,4

2,6

2,8

3,0

3,2

3,4

3,6

3,8

4,0

Particle Size (um)

Meno particellepiù piccole

Piccola quantità di particelle più grandi

min1 2 3 4 5 6 70

1.8µm: Rs(4,5) = 5.45Peak Width 5s(9) = 0.075

5µm: Rs(4,5) = 3.63Peak Width, 5s (9) = 0.103

Rs = +50%

Aumento della Risoluzione in funzione del Particle Size

+ 50% Resolution (Theory: + 67%)+ 125% Efficiency (Theory: + 177%)

Colonna SB-C18 RRHT 4.6 x150mm

Massima lunghezza

Rs(1,2) = 3.1

0.5 1 1.5 2 2.5

12 34

.

4.6 x 250 mm, 5 m1 mL/min

min0 5 10 15 20 25 30

4.6 x 100 mm, 3.5 m1 mL/min

4.6 x 30 mm, 1.8 m 1 mL/min

4.6 x 30 mm, 1.8 m2 mL/min

min0 5 10 15 20 25 30

min0 5 10 15 20 25 30

min0 5 10 15 20 25 30

29.65

12.71

4.15

2.09

1 23

1

1

2

2

3

3

4

4

4

Rs(1,2) = 4.8

Rs(1,2) = 3.5

Rs(1,2) = 3.3

N=22680

N=11691

N=6104

N=6568

N=6460

N=6463

N=21848

1100 HT + 1.8um RRHT : • Reduction of analysis time from 30 min to 2 min

• Speed: 14x faster than 4.6 x 250mm, 5um

• Time saving: 92%• Resolution: -35%

AUMENTO DELLA VELOCITA’ D’ANALISI CON HPLC 1100

Speed

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0Time (m in)

1

23

4

5 67

1

23

4

56

7

1

23

4

5 6

7

Sample: 7 AnalgesicsColumn: Zorbax RRHT SB-C18,

1.8 um, 4.6 x 30 mm

Rs2,1 = 2.1

Rs2,1 = 2.2

Rs7,6 = 2.2

Rs7,6 = 2.2

Higher flow rate:

higher speed – same resolution – higher sensitivity/precision

2 ml/min – 90bar

3 ml/min – 133bar

4 ml/min – 181bar

2x faster than 2ml/min without loss in resolution!

Ultra-fast analysis in 26 sec4 peak width ~ 0.6 sec

< 50 sec cycle time

7 analgesics separated in 21 secfor peaks 1,2,3Total time Injection-to-Injection

HPLC, 40°CSB-C184.6 x 150mm, 5µm1.20ml/min, 40°CAnalysis Time = 11min

min0 2 4 6 8 10

Rapid Resolution HT Columns – Up to 20X FasterRapid Resolution HT Columns – Up to 20X Faster

RRHT SB-C182.1mm x 50mm 1.8µm1.00ml/min, 40°CAnalysis Time= 1.1min

RRLC, 40°C 10x faster

min0.2 0.4 0.6 0.8 10

0.2 0.60 0.4 min

RRHT SB-C182.1mm x 50mm 1.8µm2.40ml/min, 95°CAnalysis Time: 0.4min

RRLC, 95°C

27x faster >20X faster>20X faster

Up to 20x faster than conventional HPLC • With same or better performance (resolution, precision, sensitivity, carry over)• Compliant with strictest regulatory performance requirements

min1 2 3 4 5 6 70

4

5

1.8µm: Rs(4,5) = 5.45Peak Width 5s(9) = 0.075

5µm: Rs(4,5) = 3.63Peak Width, 5s (9) = 0.103

Rs = +50%

NON SOLO VELOCITA’ MA ANCHE EFFICIENZA

+ 50% Resolution (Theory: + 67%)+ 125% Efficiency (Theory: + 177%)

Colonna SB-C18 RRHT 4.6 x150mm

Massima lunghezza