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microtubule severing with growth reinitiation - marco saltini

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Page 1: Microtubule severing with growth reinitiation - Marco Saltini

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Microtubule severingwith growth reinitiation

Marco SaltiniTheory of Biomolecular Matter group

AMOLF (Amsterdam)

Marco Saltini FOM Institute AMOLFMicrotubule severing with growth reinitiation

Page 2: Microtubule severing with growth reinitiation - Marco Saltini

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Microtubules of plant cellsreorient in response to the perception of blue light

Marco Saltini FOM Institute AMOLFMicrotubule severing with growth reinitiation

Page 3: Microtubule severing with growth reinitiation - Marco Saltini

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Microtubules undergo severing at their crossovers

katanin

Marco Saltini FOM Institute AMOLFMicrotubule severing with growth reinitiation

Page 4: Microtubule severing with growth reinitiation - Marco Saltini

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Microtubules undergo severing at their crossovers

katanin

Marco Saltini FOM Institute AMOLFMicrotubule severing with growth reinitiation

Page 5: Microtubule severing with growth reinitiation - Marco Saltini

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Microtubules undergo severing at their crossovers

katanin

p+ 1-p+

Marco Saltini FOM Institute AMOLFMicrotubule severing with growth reinitiation

Page 6: Microtubule severing with growth reinitiation - Marco Saltini

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The mean number of MTs grows exponentially in time

0 100 200 300 400 5000

5

10

15

20

25

30n

um

ber

of

MTs

time (sec)

WTspr1

3xeb1clasp

0 3 6 9 12

time (min)

0

0,1

0,2

0,3

0,4

0,5

fracti

on

of

exti

ncti

on

s

WTspr1

3xeb1clasp

Marco Saltini FOM Institute AMOLFMicrotubule severing with growth reinitiation

Page 7: Microtubule severing with growth reinitiation - Marco Saltini

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The mean number of MTs grows exponentially in time

0 100 200 300 400 5000

5

10

15

20

25

30n

um

ber

of

MTs

time (sec)

WTspr1

3xeb1clasp

0 3 6 9 12

time (min)

0

0,1

0,2

0,3

0,4

0,5

fracti

on

of

exti

ncti

on

s

WTspr1

3xeb1clasp

nu

mber

of

MTs

time

p+?

Marco Saltini FOM Institute AMOLFMicrotubule severing with growth reinitiation

Page 8: Microtubule severing with growth reinitiation - Marco Saltini

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Aknowledgments

AMOLF (Amsterdam)Bela MulderTheory of Biomolecular Matter group

Technische Universiteit DelftMarileen Dogterom Lab

Carnegie Institution for Science (Stanford)Jelmer LindeboomMasayoshi NakamuraDavid Ehrhardt

Marco Saltini FOM Institute AMOLFMicrotubule severing with growth reinitiation


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