microscopes compound bright- field light microscope maximum useful magnification = 1200x why?
TRANSCRIPT
Microscopes
• Compound Bright-Field Light Microscope
• Maximum Useful Magnification =
1200X• Why?
Resolution
• Useful magnification is limited by Resolution• Resolution is influenced by Numerical Aperture
(NA)• NA determined by Wavelength of Light and
Refractive Index• Improve Magnification
Shorten Wavelength
Increase Refractive Index
Staining
• Simple Stains• Light Microscope -
Colored Dye• Electron Microscope -
Electron Dense• Differential Stains
Gram Stain
Gram Stain
Most Important Stain
Key Bacteriological Test
Conventional Stain
Multiple Steps & Subjective
Fluorescent Stain
One Step & +/-
Optical Tricks
• Oil Immersion Lens
• Dark Field Microscope
Other Microscopes
Phase Contrast Microscopes
Fluorescent Microscopes
More Microscopes
• Interference Contrast Microscope• Atomic Force Microscope
Confocal Scanning Laser Microscope
Electron Microscopes
Shorten wavelength• Scanning Electron
Microscope- SEM
3 Dimensional images
•TEM•Better Resolution
Transmission Electron Microscope
Microscopes Word ListCompound Staining Confocal
Light Simple Scanning
Microscope Differential Laser
Bright-Field Gram Stain Electron
Magnification Phase Microscope
Resolution Contrast Transmission
Numerical Dark-field Scanning
Aperture Fluorescent
Oil Interference
Immersion Contrast