microscope photography with canon eos 550d ny-1s_rebel.pdf
Post on 19-Nov-2015
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First install attachment() to main unit( ) . Next, install attachment to cameras lens mount and secure.
Take out eyepiece
and install main unit().
When inside diameter of
eyepiece is 30mm, use
When unstable, try to secure with duct tape, or have support unit beneath the camera.
Insert main unit ( ) directly. When fixating screw is attached to phototube, use it to secure adapter tightly to phototube.
Screw-in C-mount sleeve
( ) to microscopes
conversion ring ( ) to
main unit(), and insert
into C-mount sleeve.
Fixate with screw at the
Install NY-JIS fixation sleeve (sold separately) to JIS phototube. Then, insert main unit( ) into it and secure with screw at the side of NY-JIS.
Compatible with direct (1.0x) c-mount only
Install NY-BH fixation sleeve (sold separately) to PT phototube. Then, Insert main unit( ) into it and secure with screw at the side of NY-BH.
Camera Preparation Connect adapter ACK-E8 to camera. p203 Connect remote switchRS-60E3 to camera. p205 Turn on the power, and set data/language. 2730 Install SD card. 31 * Please refer to cameras manual for other settings as Auto Power Off, brightness of LCD and so on.
Select Shooting Mode. For the first timer (aperture-priority) is recommended 80. When you get accustomed, try (Program)60, or (manual)83. You can select details setting according to your preferences.
Select ISO Speed. Set ISO 100-200 in normal environment. SetISO 400-800 in relatively dark environment.Higher the ISO, there appear to be more noise in the image captured. 62
Enable Live View Shooting. UnderLive View function settings, set Live View shoottoEnable. 109 Output live view to TV monitor with
AV Cable is also possible.
Always try focusing, while watching image on LCD. There is difference in focus between the image you see in eyepiece and image on camera. When it is focused, press shutter on remote switch.
You can focus through view finder as well.
Other Settings Please adjust below settings for capturing optimum images. White Balance 99 * default setting is AUTO.
Adjust white balance manually according to color of light source * When live view image has flickering, please set white balance other than AUTO, or turn the camera power off, and restart live view under the designated lighting environment.
Exposure Compensation 87/89
When images captured are too dark or too bright set exposure compensation. It is done only by shutter speed since aperture adjustment is disabled from adapter connection.
Picture Style 91
Customize color, contrast and other picture styles as you like. You will be able to capture more vivid images by setting high in sharpness
*2For more information about remote live view feature, please refer to EOS Utility manual instruction.
*1 When live view image has flickering, please set white balance other than AUTO, or turn the camera power off, and restart live view under the designated lighting environment.
Connect camera and PC using USB cable (standard accessory) and then start up EOS Utility by turning on Camera. ClickCamera setting / Remote shooting
Shoot with MF or mode (program AE. Try focus on microscope while watching PC display. Double click white square frame on the display, enlarged image will show up. You can focus more in detail.
Once focused, Press shutter button
Remote Live View is a convenient feature that enables you to control camera remotely from your PC with EOS Utility installed. It enables you to capture high quality images by focusing while watching the PC display. Also you can check captured images with large screen right after shot is taken.
You can enlarge part of image while focusing.
EOS Utility / PC System requirement Please install software if your PC meets these requirements.
Cameras setting criteria
From set-up menu, click Live view movie function set, and click Enable on next.
Click theLive View shoot Remote Live View window appears
Save destination ISO
Image recording quality
Playback with LCD display
Take out SD card from camera and insert it into PC slot, open the file and display. You can also connect camera and PC with USB cable and transfer data. If software which is standard accessory of camera is installed already, you can transfer data automatically. In remote live view shooting, you can select save destination. to PC / to SD card / to PC&SD
When you press the PLAY button, the last captured image will be displayed 58. detail on 155. button for back and forth, + button for enlarge and shrink the images.
Playback with PC display
In the following situations
Display is black even with camera power on- Live view might be set off. Refer to manual P96 make sure live view feature is set on. - When setting manually, image will blackout if there is not enough light. Step by step, try to set ISO higher and lengthen the shutter speed so the image will be bright enough.
- Please make sure you are using the correct optical path. It might be that image is not reaching the camera lens. It happens mostly with trinocular microscopes with changeable optical path. Flickering on image - Flickering may appear on the image when white balance or exposure is set on auto. In this case, please set manually. Flickering should disappear.
- Turn off the camera, and turn on microscope light, then again turn on the camera. Image is too bright / too dark - Adjust exposure. In program mode adjust with exposure compensation, + button produces brighter, and button, produces darker images. In manual mode, longer shutter speed produces brighter and shorter shutter speed produces darker images.
- High ISO produces brighter and low ISO produces darker images. Normally ISO should be set around 200.
Grainy image - When ISO is set auto, sensitivity may rise automatically, resulting in a grainy image. In that case, set ISO manually around 100400.
Out of focus - Except for a few microscopes, there is difference in focus between image you see in eyepiece and image on camera. Please focus while watching cameras live view image.
- It may be suffering from hand movement. Try using remote control or self-timer to stabilize.
Fluorescence photography When trying fluorescence photography, please take following tips into account. - Set ISO higharound 400-800 should help you detect even weak light excitation. - Slow shutter speedslower shutter speed provide brighter image. - StabilizeTry using remote control, or self-timer to avoid blurriness of hand movements. - Connect to TV monitor/PC, and try focus on large display.
S11CF Ocular Micrometer for Digital Camera Adapter (scale 10mm / 100 split / pitch 0.1mm) Using together, S11CF enables you to capture image with scale.This special micrometer omitted the numbers entirely and moved scale to the periphery for clearer view.
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