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Microscope Biology 101 Mrs. Miller

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Page 1: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

MicroscopeBiology 101Mrs. Miller

Page 2: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

Types of Microscopes Light microscope

Compound lenses to magnify objects. Lenses refract light to make object appear

closer. 40x, 100x, 400x

Stereoscope Allows binocular viewing of larger

specimens.

Page 3: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

Types of Microscopes Scanning Electron Microscope

Use electrons to magnify objects instead of light waves.

Can magnify up to two million times. Transmission Electron Microscope

Uses electrons as well. Electrons are passed through very thin

specimens.

Page 4: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

Lenses Three magnification lenses

Scanning 4x magnification

Low power 10x magnification

High power 40x magnification

Page 5: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

Lenses Ocular lens

In addition to the magnification lenses. 10x magnification

Page 6: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

Magnification To find the total magnification multiple

the magnification of the lens by the magnification of the ocular lens.

Magnification

Ocular Lens Total Magnificatio

n

Scanning 4x 10x 40x

Low Power 10x 10x 100x

High Power 40x 10x 400x

Page 7: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

Focusing1. Always start with the scanning

objective.1. Coarse knob to focus.

2. Once you’ve focused on scanning, switch to low power.

1. Coarse knob to refocus.

3. Switch to high power.1. Fine knob to refocus.

Page 8: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

Focusing Can’t see the specimen?

Is it too light or too dark? Adjust the diaphragm.

See a line? It is a pointer

Useful for pointing things out to partner or teacher.

Try twisting the eyepiece

Page 9: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

Drawing Specimens Use pencil. Drawing should be large enough to see

detail. Label items in drawing.

Write what magnification you are viewing the object at.

Label the object.

Page 10: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

Drawing SpecimensAmeba (400x) Nucleus

Cell Membrane

Page 11: Microscope Biology 101 Mrs. Miller. Types of Microscopes Light microscope Compound lenses to magnify objects. Lenses refract light to make object

Clean Up & Storage Make sure the scanning lens is in

place. Wrap cords around the base. Cover microscope. Wash and dry slides before placing

them back in slide box. Cover slips are to be thrown away.


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