The 7th Japan-Taiwan-Korea (JTK) International Symposium on Microbial Ecology (Oct 17-19) and the 30th Annual Meeting of Japanese Society of Microbial Ecology (JSME2015, Oct 17-20)

Final Circular Dear participant, Welcome to the 7th JTK Symposium and JSME2015! This document contains scientific program and some practical information.

Venue The symposium and meeting venue is the Tsuchiura City Kijo Plaza (), 2-16-4, Chuou, Tsuchiura, Ibaraki, 300-0043 Japan. Tourist Guide map is available from: http://www.city.tsuchiura.lg.jp/data/doc/1305536264_doc_26_0.pdf You can go to the venue by bus. Take the bus at the terminals, 3, 4, or 5 in front of the Tsuchiura station and get off at the third stop ( Kijo Kouen-mae; 170 yen). The bus time table is available at http://kantetsu.co.jp/bus/timetable_tsuchiura.html .

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Microbes and

Climate Change

Contents The floor guide of the venue P-2 P-3 Best Poster Award P-26 Instructions for speakers and poster session P-3 Poster display room P-27 P-29 Symposium and oral session time table P-4 P-15 Organizing committee P-30 P-31 Banquet and route map P-11 Other reminders P-31 Chairs of oral sessions P-11 Transport information P-32 P-35 List of posters P-16 P-27

Tsuchiura City Kijo Plaza

See p-11 for details

The floor guide of the venue

The 1st floor The 2nd floor

The 3rd floor The 4th floor

Date Room 7th JTK JSME2015

Oct 17 (Sat)

1 F Foyer A Registration (12:00-17:00)

2F

Room 100B Student session (13:00-14:30)

Hall 300 Young scientist sessions (15:00-17:00)

Opening address (17:10-17:20) Keynote lecture (17:20-18:20) / Welcome event (18:20-18:45)

1F Room 100A Wakate () session (10:00-12:00)

4F Room 70 M&E Business meeting (9:00-11:00) Council meeting (11:00-13:00)

1F Foyer B Welcome reception (18:45-20:00)

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Date Room 7th JTK JSME2015

Oct 18 (Sun)

2F Hall 300 Plenary lectures (9:30-10:20, 13:00-13:50)

Main sessions (10:30-17:45) 1F Room 100A Group activity (Biofilm) (18:00-21:00) 1F Room 100B Group activity (Anaerobic world) (18:00-21:00)4F Room 70 Group activity (Outreach activities)

(18:00-21:00) 4F Room 60 Business meeting (18:00-19:00) Bioinformatics tutorial (Registered

participants only) (9:30-12:00) 2F PS Rooms 1, 2

Poster exhibition (9:20-18:00) 4F PS Rooms 3, 41F Foyer A Registration (9:00-17:00), JSME Inter-high school poster exhibition (10:20-16:30)

Oct 19 (Mon)

2F Hall 300 Symposium sessions (9:10-12:00) and Oral sessions (16:00-18:00)

1F Room 100A 1F Room 100B 4F Room 70 Oral session (16:00-18:00) 4F Room 60 Oral session (16:00-18:00) 2F PS Rooms 1, 2

Poster exhibition (9:10-16:00) 4F PS Rooms 3, 41F Foyer A Registration (9:10-17:00)

Oct 20 (Tue)

2F Hall 300 Symposium sessions (9:10-16:00) and closing remarks (16:00-16:10) 1F Room 100A

Symposium sessions (9:10-16:00) 1F Room 100B 4F Room 70 Symposium sessions (9:10-12:00) 1F Foyer A Registration (9:10-14:00)

Instructions for speakers For the speaking sessions, you will come to the front of the room at the beginning of the session. While the speaker before you is speaking, you will have a few minutes to set up your laptop (VGA connection will be provided). There will be a volunteer at the podium to help you if you need it. Time limits

Presentation Questions & answers Keynote speaker 50 min 10 min Plenary speakers 40 min 10 min JTK Young session speakers 30 min 10 min JTK Main session speakers 20 min 5 min JTK/JSME Oral session speakers 12 min 3 min JSME Symposium session speakers 17 min 3 min

During your talk, you will receive a signal at 5 minutes-to-go, then a second signal at 2 minutes-to-go, and at the end of the above presentation time slot. Time limits are critical and need to be followed in order to keep the symposium on schedule and to ensure a fair go for other speakers and the audience. The Session Chair will help ensure that you stay on schedule during your presentation. Poster session Posters should fit in DIN A0 portrait format (width: 84.1 cm, height 118.9 cm). All posters remain on display, Sunday - Monday for continuous viewing. Please hang your poster on Sunday morning and remove it by Monday afternoon, not before 16:30. Supplies will be available for hanging posters. We kindly ask the presenting authors for their presence:

authors of posters with odd numbers, Sunday, 15:20 - 16:20 authors of posters with even numbers, Monday, 15:00 - 16:00

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Cameras and Recording Audio/video recorders, cameras, and photographs are not allowed in session rooms or in the exhibit and poster areas. Time Table Oct 17 (Saturday)

Time Room Session 10:00-12:00

Room 100A

JSME Wakate () Session Satoshi Tamazawa, AIST, Japan

Yuya Sato, AIST, JapanHiroyuki Kusada, AIST, Japan

Yumi Mori, Tohoku University, JapanTomohiro Inaba, AIST, Japan

12:00-13:00 Lunch Break

13:00-14:30

Room 100B

JTK Student Session, World Caf Kenshi Suzuki, Shizuoka University, Japan

Yi-Lung Chen, National Taiwan Normal University, TaiwanSungeun Ahn, Korea University, Korea

14:30-15:00 Break

15:00-17:00

Hall 300

ISME-JTK Young Scientist Session, Frontiers in Microbes and Environments

IYS-1 Fascinating microbes in extreme environments Ryosuke Nakai, National Institute of Genetics, Japan

IYS-2 Cyanophycin mediates the accumulation and storage of fixed carbon in non-

heterocystous filamentous cyanobacteria from coniform mats Biqing Liang, National Cheng Kung University, Taiwan

IYS-3 Ecological characterization of methanogens and methanotophs in rice paddies Hyo Jung Lee, Chung-Ang University, Korea17:00-17:10

Opening Remarks Hiroyuki Ohta, Chair of 7th JTK and JSME2015

17:20-18:20

KPL-1 Keynote Lecture Chair, Satoshi OkabeMulti-omics illuminates permafrost soil microbiome response to climate warming

Janet K. Jansson, ISME President18:20-18:45 Welcome event

18:30-20:00 Foyer B Welcome reception

Time Room Business meeting 9:00-11:00 Room 70 M&E Editorial BoardM&E 11:00-13:00 Room 70 JSME Council

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Oct 18 (Sunday), morning Time Room Session

9:30-10:20 Hall 300

KPL-2 Plenary Talk 1 Chair, Kiwamu MinamisawaDark Energy Ecosystem - Looking through the modern deep ocean to the ancient and

the extraterrestrial ones Ken Takai, JAMSTEC, Japan

10:20-10:40 Break, JSME Inter-High School Poster Session (1) (10:20-11:20)

10:40-12:05 Hall 300

JTK Main Session (Part 1), Microbes and Climate Change

IMS-1 Organic matter-microbe interactions in the ocean - Towards a global synthesis Toshi Nagata, The University of Tokyo, Japan

IMS-2 Eco-friendly approaches based on biological control to suppress harmful algal

blooms Yoon-E Choi, Korea University, Korea

IMS-3 Evolution of vertebrate symbiotic gut microbiota: the story starts from tadpoles Chun-Yao Chen, Tzu Chi University, Taiwan

Time Room Session

9:30-12:00 Room 60

Bioinformatics tutorial (Registered participants only) Japanese consortium for human microbiome (JCHM)

2000 16S DNA 16S

Time Room Session

12:05-13:00 Hall 300

Luncheon Symposium Promoting globalization and gender equality in higher education and research environment among Asian countries

Organizers: JSME Committee for the Promotion of Diversity and Gender Equality & JTK Woman Scientist Network Chair: Wakako Ohtsubo, Tohoku University

Current issues and prospective: International research activities and gender equality in Japan and JSME

Natsuko Hamamura, Kyushu University, Japan Gender equality and woman microbiologists in research environment in Taiwan

Shir-Ly Huang, President of TSME, National Central University, TaiwanJuei-Yu Chiu, National Pingtung University of Science and Technology, Taiwan

The gender equality in research environments - past, present and future perspectives

Kyunghwa Baek, National Marine Biodiversity Institution of Korea, Korea Roundtable discussion: Sharing experiences for successful international activities and encouraging young scientists in Asian microbial ecological research

Panelists: JSME: Mia Terashima (Hokkaido Univ), Natsuko Hamamura (Kyushu Univ) TSME: Shir-Ly Huang (National Central Univ), Juei-Yu Chiu (National Pingtung

University of Science and Technology), DV Chang (Soochow Univ), Shiu Mei Liu (National Taiwan Ocean Univ)

KSM: Kyunghwa Baek (MABIK), Sunja Cho (Pusan National Univ)

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Oct 18 (Sunday), afternoon Time Room Session

13:00-13:50 Hall 300

KPL-3 Plenary Talk 2 Chair, Hiroyuki OhtaExtreme halophiles and carbon monoxide: looking through a window at Earth's past and towards a future on Mars

Gary M. King, Louisiana State University, USA13:50-14:00 Break

14:00-14:55

Hall 300

JTK Main Session (Part 2), Microbes and Climate Change

IMS-4 Characterization and Immobilization of an exoelectrogenic bacterium Chang-Ping Yu, National Taiwan University, Taiwan

IMS-5 A comprehensive approach to explore metabolic and phylogenetic diversity of bacterial steroid degradation

Yin-Ru Chiang, Academia Sinica, Taiwan14:55-15:10

Promotion of Microbes and Environments

15:00-16:20, 16:50-17:10

Foyer A JSME Inter-High School Poster Session (2) (15:00-16:20) and Poster Award Ceremony (16:50-17:10)

15:20-16:20

PS Rooms 1-4 JTK and JSME Poster Session (posters with odd numbers)

16:20-17:45 Hall 300

JTK Main Session (Part 3), Microbes and Climate Change IMS-6 Land use alters the structure and function of soil microbial community

Kyunghwa Baek, MABIK, KoreaIMS-7 Nitrous oxide production by soil ammonia-oxidizing Thaumarchaeota

Man-Young Jung, Chungbuk National University, KoreaIMS-8 N2O emission from soybean rhizosphere and its mitigation by Bradyrhizobium

diazoefficiens inoculation Manabu Itakura, Tohoku University, Japan

Oct 18 (Sunday), evening

Time Room Session

18:00-21:00 Room 100A Group activity (Biofilm) Room 100B Group activity (Anaerobic world) Room 70 Group activity (Outreach activities)

Time Room Business meeting

18:00-19:00 Room 60 JTK business meeting

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Oct 19 (Monday), morning (1) Time Room Session

9:10-10:30

Hall 300

JS1 Microbiology of the Nitrogen Cycle Cristina Snchez

JS1-1 Rhizosphere is a textbook for microbial N cycle Kiwamu Minamisawa, Tohoku Univ.

JS1-2 Regulation of N2O reductase genes by the two-component system NasST in Bradyrhizobium diazoefficiens

Cristina Snchez, Tohoku Univ.JS1-3 Phylogenetic diversity and environmental distribution of denitrifying fungi

Reiko Sameshima-Saito, Shizuoka Univ.JS1-4 Isolation, physiology, and genomics of novel nitrifiers

Hirotsugu Fujitani, Waseda Univ.

Room 100A

JS4 ,

JS4-1 ,

JS4-2 ,

JS4-3 ,

Room 100B

JS5 ,

JS5-1 ,

JS5-2 type I ,

JS5-3

, JS5-4

,

Room 70

JS3 Microbial pathogen ecology Life is beautiful Satoshi Ishii, Fumito Maruyama

JS3-1 Novel transferable multi-drug resistance plasmid and macrolide resistance genes from Photobacterium damselae subsp. damselae isolated from seawater of an aquaculture site

Lisa Nonaka, Dokkyo Medical Univ.JS3-2 Diversity in mycobacteria: Distribution, host specificity, morphology and

genome Yukiko Nishiuchi, Osaka City Univ. Medical Sch.

JS3-3 Most chlamydiae live in natural environments but not in humans. - What kinds of features do they need to cause human disease?

Kasumi Ishida-Kuroki, Nippon Medical Sch.JS3-4 Genetic profiles of Bacillus anthracis indigenous to Zambia and

characterization of Bacillus species causing anthrax-like disease Naomi Onishi, Hokkaido Univ.

10:30-10:40 Break

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Oct 19 (Monday), morning (2) Time Room Session

10:40-12:00

Hall 300

JS2 Linking between microbial ecology and ecosystem function: compartmentalization of microbial diversity and environmental gradients

Natsuko Hamamura, Taichi YokokawaJS2-1 Evaluating community-environment relationships along fine to broad

taxonomic resolutions reveals evolutionary forces underlying community assembly

Hsiao-Pei Lu, National Taiwan Univ.JS2-2 Links between structure and function of aquatic bacterial communities

Taichi Yokokawa, JAMSTECJS2-3 Applications of nonlinear time series analysis to microbial ecology studies

Masayuki Ushio, Ryukoku Univ.JS2-4 Soil aggregate structure and its implications for microbial ecology:

location and quality of substrates for heterotrophs Rota Wagai, NIAES

Room 100A

JS8 ,

JS8-1 -Burkholderia

JS8-2 ,

JS8-3 ,

JS8-4 ,

Room 100B

JS14 --

JS14-1 Candidatus Methanogranum caenicola: Group E2

, JS14-2

, JS14-3

, JS14-4

,

Room 70

JS9

JS9-1 ,

JS9-2 ,

JS9-3

, JS9-4

, ()

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Oct 19 (Monday), afternoon (1)

Time Room Session 13:00-14:00 Hall 300

JSME Society meeting

14:00-15:00 Hall 300

JSME Award lectures 2015 Microbial Ecology in Soil, Water, and Human Health , Univ. Minnesota 2015 The encounter with light, Rhodopsin, Microbe and JSME , 2015 M&E Low Nitrogen Fertilization Adapts Rice Root Microbiome to Low Nutrient Environment by Changing Biogeochemical Functions

Seishi Ikeda et al.15:00-16:00

PS Rooms 1-4 JTK and JSME Poster Session (posters with even numbers)

16:00-18:00

Hall 300

JSME Oral session I: Genomics/Methods

OH-01 Brevimastigomonas sp. KY003 ,

OH-02 mef(C)mph(G) ,

OH-03 SbtA DUF2237 ,

OH-04 Nitrospira ,

OH-05 MAPLE Ver.2.1 , JAMSTEC

OH-06 Large-scale metagenomic analysis of the Japanese gut microbiome ,

OK-07Toward ecosystem-scale biodiversity monitoring: Metabarcoding of microbial and environmental DNA from identical water samples

Wataru Iwasaki, Univ. Tokyo

OK-08 1 PCR 16S rRNA ,

Room 100A

JSME Oral session II: Symbiosis/Extreme/Physiology-Metabolism

OJ-09 ,

OJ-10 Burkholderia ,

OJ-11 Metagenomic analysis of bacteria associated with soybean nodules in continuous cropping field

Hirohito Tsurumaru, Tohoku Univ.

OJ-12 The Nod factor-independent and T3SS-dependent nodulation in Rhizobiumlegume symbiosis

Shin Okzaki, Tokyo Univ. Agric. Technol.

OF-13 2 16S rRNA Haloarcula

,

OF-14 Nostoc sp. HK-01 ,

OI-15 Release from catabolite repression and enhancement of butanol production in solventogenic Clostridium beijerinckii SBP2-HB by ammonium acetate

Miho Kanemoto, Tokyo Univ. Agric. Technol.

OI-16 - The importance of fatty acid synthesis in colony formation

,

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Oct 19 (Monday), afternoon (2)

Time Room Session

16:00-18:00

Room 100B

JTK/JSME Oral session III: Soil/Environmental Hygiene

ON-17Assessment of bacterial effect following Asian dust events through comparison with seasonal variability in airborne bacterial community

Jonguk Park, Osaka Univ.

ON-18Clostridium

,

OB-19 ,

OB-20 (JTK0041)

Spatial distribution and community structure of soil protists in the rice rhizosphere revealed by a Mini-rhizobox experiment

Rasit Asiloglu, Nagoya Univ.

OB-21 ,

OB-22 DNA ,

OB-23 Ralstonia solanacearum ,

OB-24

Uptake of a chitin-degradation product via the constitutive N-acetylglucosamine N,N-diacetylchitobiose binding protein NgcESCO of an ABC transporter in Streptomyces coelicolor A3(2)

Akihiro Saito, Shizuoka Inst. Sci. Technol.

Room 70

JTK/JSME Oral session IV: Biogeochemical Cycling/Hydrosphere (1)

OE-25Electron uptake of sulfate reducing bacteria by using outer-membrane cytochromes

Akihiro Okamoto, Univ. Tokyo

OE-26Anaerobic ammonium oxidation coupled with extracellular electron transport by Shewanella oneidensis MR-1

Junki Saito, Univ. TokyoOE-27(JTK0046)

Novel example of CO2 cathodic driven bioreduction by Shewanella oneidensis MR-1: possible implications for carbon cycling in deep oceans

Eugenio La Cava, Univ. TokyoOA-28(JTK0036)

Responses of coastal microbial communities to monomeric and polymeric chemical compounds

Md Nurul Haider, Univ. Tokyo

OA-29 Seasonal change of surface layer bacterial community in Sendai Bay ,

OA-30Genomic analysis of coral-associated bacteria to understand coral-bacteria interactions

Yusuke Okubo, Waseda Univ. OA-31 (JTK0037)

Distribution patterns of Verrucomicrobia along a meridional transect across the Pacific Ocean

Shotaro Suzuki, Univ. Tokyo

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Oct 19 (Monday), afternoon (3)

Time Room Session

16:00-18:00 Room 60

JSME Oral session V: Hydrosphere (2)

OA-32 Chloroflexi

,

OA-33 UASB

,

OA-34 ,

OA-35 ,

OA-36 nxrB , JAMSTEC

OA-37

, JAMSTEC

OA-38

,

Chairs of oral sessions

OH-01

OF-14

OE-27 (JTK0046) Mamoru Oshiki OH-02 OI-15 OA-28 (JTK0036)

Akihiro Okamoto

OH-03 OI-16 OA-29 OH-04 ON-17

Akihiro Saito

OA-30 OH-05

ON-18 OA-31 (JTK0037) OH-06 OB-19 OA-32

OK-07 OB-20 (JTK0041) OA-33 OK-08 OB-21

OA-34 OJ-09

OB-22 OA-35 OJ-10 OB-23 OA-36

OJ-11 OB-24 OA-37 OJ-12 OE-25

OA-38

OF-13 OE-26 Oct 19 (Monday), evening

Time Venue 19:00-21:00 Hotel Marroad Tsukuba Banquet

The hotel will offer free shuttle buses for the hotel, but the seating capacity is limited (about 60). For young people, please take a walk to the hotel.

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700 m

Tsuchiura Station

Oct 20 (Tuesday), morning (1) Time Room Session

9:10-10:30

Hall 300

JS7 ,

JS7-1 ,

JS7-2 Zymomonas mobilis ,

JS7-3 ,

JS7-4 ,

Room 100A

JS12 ,

JS12-1 ,

JS12-2 CRISPR

,

JS12-3 Chaetoceros tenuissimus

,

JS12-4 dsRNA RNA

,

Room 100B

JS20

JS20-1 ,

JS20-2 ,

Room 70

JS6 2000 ,

JS6-1 2000 ,

JS6-2 2000 ,

JS6-3 Nitrogen mineralization in soils and its relationship with microbial community diversity along a vegetation gradient in Miyakejima island

,

JS6-4 The development of soil microbial community during vegetation recovery and pedogenesis on the new volcanic deposits of Miyake-jima

,

10:30-10:40 Break

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Oct 20 (Tuesday), morning (2)

Time Room Session

10:40-12:00

Hall 300

JS18 ,

JS18-1 CO2

, ()

JS18-2

,

JS18-3 ,

JS18-4

,

Room 100A

JS22 ,

JS22-1 ,

JS22-2 :

JS22-3 ,

JS22-4 Ophiocordycipitaceae , NBRC

Room 100B

JS21

JS21-1 yet-to-be cultured bacteria ,

JS21-2 ,

JS21-3 ,

Room 70

JS16 ,

JS16-1

,

JS16-2 ,

JS16-3 ,

JS16-4 ,

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Oct 20 (Tuesday), afternoon (1)

Time Room Session

13:00-14:20

Hall 300

JS11 ,

JS11-1 ,

JS11-2 ,

JS11-3 ,

JS11-4 , JAMSTEC

Room 100A

JS15 ,

JS15-1 ,

JS15-2 Microcystis aeruginosa

,

JS15-3

,

JS15-4

,

Room 100B

JS13

JS13-1

,

JS13-2 ,

JS13-3

,

JS13-4 , ()

14:20-14:40 Break

14:40-16:00 Hall 300

JS10 ,

JS10-1 Predator-prey relationship in microbial food web in the deep North Pacific ,

JS10-2 ,

JS10-3

,

JS10-4 ,

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Oct 20 (Tuesday), afternoon (2)

Time Room Session

14:40-16:00

Room 100A

JS19 ,

JS19-1 ,

JS19-2 , NBRC

JS19-3 ,

JS19-4 Cinchona spp.

,

Room 100B

JS17

JS17-1 ,

JS17-2 (micro-ecomics) ,

JS17-3

,

JS17-4 ,

16:00-16:10 Hall 300 Closing remarks

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List of posters

Room Session

PS Room 4 (4th floor)

Aquatic ecosystem PA-001 (JTK)

The dynamics of bacterial diazotrophic communities within the corals Acropora tenuis and Pocillopora verrucosa in Okinawa shallow reef, Japan

Muhammad Wahyudin Lewaru

PA-002 Identification and genomic analysis of temperate Pseudomonas bacteriophage PstS-1 from the Japan Trench at a depth of 7000 m

Mitsuhiro Yoshida

PA-003 High abundance of heterotrophic nanoflagellates in the oxic-anoxic interface of meromictic Lake Suigetsu, Japan

Indranil Mukherjee (Shin-ichi Nakano*)

PA-004 Impact of migratory birds on the seasonal dynamics of pathogenic bacteria population in a freshwater lake

Mayumi Kobayashi

PA-005 Metagenomic analysis of viral communities in Osaka bay, Japan Hiroyasu Watai

PA-006 (JTK)

The diversification of bacterial populations in wetland habitats: studies on the upper course of Gum-Ho River and its tributary

Young Ok Lee

PA-007 Occurrence and fate of Synechococcus in the dark hypolimnion in deep freshwater Lake Biwa

Hiroyuki Takasu

PA-008 Current systems shape bacterial community structure in the Sanriku coastal area, northwestern Japan

Ryo KanekoPA-009 (JTK)

The diversity of endophytic fungi from coastal plants in Taiwan Yu-Hung Yeh

PA-010 Microbial diversity of Marimo in Lake Akan Ryosuke Nakai

PA-011 Bacterial diversity of eutrophic lake wetland in Wuliangsuhai Ji Zhao

PA-012 Characterization of degradation of wetland plant communities on floodplain in typical steppe region of Inner Mongolia Plateau

Li-Xin Wang

PA-013 Effect of temperature change on microbial community structure in groundwater during long-term thermal loading experiments

Satoshi Ohkubo (Jun-ichi Ohnishi*)

PA-014 Structure, plasticity and robustness of microbiome in the colonies of a reef coral Acropora tenuis

Michihiro Ito

PA-015 -

-

PA-016 454

PA-017 (JTK)

PA-018

PA-019 N2O production by ammonia-oxidizing bacteria from mesotrophic sediments in coastal environments

Daisuke Imaizumi (Tatsunori Nakagawa*)

PA-020 Down-flow Hanging Sponge

*Corresponding author

P-16

List of posters continued

Room Session

PS Room 4 (4th floor)

Aquatic ecosystem (continued)

PA-021 ( *)

PA-022

PA-023 ( *)

PA-024

PA-025

Soil ecosystem

PB-026 Bacterial community shift along the primary soil horizons on the recent volcanic deposits in the Island of Miyake (Miyake-jima), Japan

Akinori Hirano

PB-027 Who produces N2O in cropland fields? Kazuo Isobe

PB-028 Measurement of Fusarium proliferatum by real-time PCR method

PB-029 (JTK)

Metagenomic analysis of paddy soil microbial community to reveal methane emission mechanisms

Tatsuya Unno

PB-030 (JTK)

Rhizosphere community analysis of tomato plants and characterization of plant growth promoting Flavobacterium strains

Soo Yeon Choi

PB-031 N2O reductase gene and activity in bradyrhizobia nodulating Aeschynomene indica

PB-032 Effects of arbuscular mycorrhizal fungi on the growth and Ce uptake of maize grown in Ce-contaminated soils

Wei Guo

PB-033

PB-034 How are you living, Verrucomicrobia? What's your life mode like? ( *)

PB-035 NH4+36 150 MAOM MPN

( *)

PB-036 nirK N2O

PB-037

PB-038

PB-039

PB-040 ( *)

PB-041

PB-042

PB-043 -

*Corresponding author

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List of posters continued

Room Session

PS Room 4 (4th floor)

Soil ecosystem (continued)

PB-044

PB-045

Forest ecosystem

PC-046

PC-047

PC-048

PC-049

PC-050 (JTK)

Pyrosequencing-based assessment of bacterial community structure in different layers of the oak forest soil

Song-Ih HanWaste water treatment

PD-051

PD-052 Gemmatimonas aurantiaca (N2O)

PD-053 EGSB

PD-054 Accumulibacter

PD-055

PD-056

PD-057 1,4- ( *)

PD-058

PD-059

PD-060 (JTK)

Nitrosomonas europaea

PD-061 (JTK)

nirS

PD-062 (JTK)

Ecotoxicological and chemical evaluation of effluents from a petroleum chemical plant and the receiving coastal water

Shiu Mei Liu

PD-063 Detection of fermentative dissimilatory nitrate reduction to ammonium bacteria in municipal wastewater treatment plants in Japan

Pokchat Chutivisut

PS Room 3 (4th floor)

Material cycles

PE-064 Nitrogen fixation depends on the methane oxidation by Methylosinus sp. isolated from paddy rice roots

Ryo Shinoda

PE-065 N2O emission by denitrifying bacteria isolated from freshwater environments by using a medium with low nitrate

Mitsunori Tarao*Corresponding author

P-18

List of posters continued

Room Session

PS Room 3 (4th floor)

Material cycles (continued) PE-066 (JTK)

Effects of elevated CO2 and salinity on microbial community and biogeochemical processes in a salt marsh ecosystem

Hojeong KangPE-067 (JTK)

Cr(III) oxidation potential of manganese biooxides by Mn-oxidizing bacteria Juei-Yu Chiu

PE-068 n-

PE-069 X BIOS

PE-070

PE-071

Extreme environment

PF-072 RNA-Seq provides insights into the novel carbon monoxide metabolism in Carboxydothermus pertinax

Yuto Fukuyama

PF-073 Isolation of novel hyperthermophilic archaeal virus, Pyrobaculum filamentous virus 1 Tomohiro Mochizuki

PF-074 Active bacterial microbiota in the gut of glacier invertebrates as revealed by 16S rRNA deep sequencing analysis

Takumi Murakami

PF-075 Methane oxidation potential of arctic wetland soil of taiga-tundra ecotone in northeastern Siberia

Jun MurasePF-076 (JTK)

Elucidation of trophic difference by fatty acid analysis of bivalve lipids Hiroaki Saito

PF-077 ( *)

PF-078

PF-079

PF-080

PF-081

PF-082 DAPI ( *)

PF-083

PF-084 Nostoc sp. HK-01

PF-085 ( *)

PF-086 (JTK)

Multilocus sequence typing (MLST)

PF-087

PF-088

*Corresponding author

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List of posters continued

Room Session

PS Room 3 (4th floor)

Phylogeny/Taxonomy

PG-089 A study on the yeast Saccharomyces cerevisiae isolates from the Shirakami Mountains, with a focus on their phylogenetic traits

Yuriko Moriyama

PG-090 Isolation and characterization of rhodopsin containing Mesoflavibacter misakiensis SK-14T sp, nov. from sea surface microlayer at the coast of Misaki, Japan

Sanghwa Park

PG-091 A novel bacterial species of yet unidentified genus isolated from thermophilic compost prepared from marine animal resources material

Fui Seung Clament Chin (Kenji Sakai*)

PG-092 0.22 m

PG-093 Marinifilum

PG-094 Thiogranum longum

PG-095 Suigetsumonas clinomigrationis

PG-096 II Lachnospiraceae

PG-097 Deferribacteres

PG-098 Bacilli

PG-099 oligotrophic Nitrosospira sp.

PS Room 4 (4th floor)

PG-101

PG-102 Acidobacteria F183

PG-103

PG-104

Genome

PH-105 Sphingomonas pYAN-2 DNA ( *)

PH-106 A viral proteomic tree server and its application to a marine metagenomic study Yosuke Nishimura

PH-107 Molecular analysis of octylphenol degradation by Sphingobium amiense strain YT Mina Ootsuka

PH-108 Whole genome analysis of non-phototrophic, thallium-tolerant Rhodopseudomonas sp. SK50-23

Zhihua Bao

PH-109

Genomic adaptation of the thermophilic hydrogenogenic carboxydotrophic bacterium from marine sediment, Calderihabitans maritimus KKC1, driven by carbon monoxide dehydrogenases

Kimiho Omae

PH-110 Quality control of bacterial single-amplified genome sequences Toru Maruyama*Corresponding author

P-20

List of posters continued

Room Session

PS Room 4 (4th floor)

Genome (continued) PH-111 Evaluation of whole genome amplification methods for bacterial single-cell genomics Keisuke Yamagishi

PH-112 (JTK)

Genome analysis of branched chain fatty acid degrading syntrophs: Syntrophomonas wolfei subsp. methylbutyratica strain JCM 14075T and Syntrophothermus lipocalidus strain TGB-C1T

Takashi Narihiro

PH-113 A GenomeNet resource for virus-host interaction network analyses Tomoko Mihara

PH-114 Comparative genomics of endomicrobial symbionts associated with distinct protist species in the termite gut

Kazuki Izawa

PH-115 Complete genome sequence of a novel endobacterium Mycophilus cysteinexigens from soil fungus Mortierella elongata FMR23-6

Tomoyasu Nishizawa

PH-116 (JTK)

Investigations into the roles of symbiotic treponemes in the higher termite Italic: Nasutitermes takasagoensis

David Starns

PH-117 Identification and characterization of the soil-specific expression genes in Pseudomonas putida

Hajime Morimoto (Yasuhiro Kasahara*)

PH-118 Metagenome-mapping method discriminates native and inoculant populations of soybean bradyrhizobia in agricultural soil

PH-119 (JTK)

Microbial genome of Sphingobium chungbukense DJ77 and production of polyhydroxybutyrate from 4-methoxybenzoate

Jong-Chan Chae

PH-120 Functional classification of uncultured Ca. Caldiarchaeum subterraneum using the Maple System

Hideto Takami

PH-121

PH-122 ( *)

PH-123

PH-124 pSC101 ( *)

PH-125 Mycoplasma mobile

PH-126

Physiology/Metabolism

PI-127 Physiological and phylogenetic characterization of bacteriochlorophyll-containing oligotrophs isolated from river biofilm

Setsuko Hirose

PI-128 Transcriptome analysis of Microcystis aeruginosa during Ma-LMM01 infection Daichi Morimoto

PI-129 Random mutation enhances degradation of benzaldehyde by Rhodococcus jostii RHA1 Hong-Mao Yang

PI-130 Ecological significance of chlorophyll f within a microbial mat Satoshi Ohkubo

PI-131 Molecular mechanisms for sensing and responding to electrode potentials in Shewanella oneidensis MR-1

Atsumi Hirose

PI-132 Operational taxonomic units (OTUs)-based analysis of swine gut microbiota in response to the treatment with tylosin, an antibiotic growth promoter (AGP)

Jungman Kim*Corresponding author

P-21

List of posters continued

Room Session

PS Room 4 (4th floor)

Physiology/Metabolism (continued)

PI-133 Ammonium acetate increases the production of butanol by Clostridium beijerinckii cultures growing in sweet sorghum juice

Kent Uehara

PI-134 Effect of nanobubbles on growth and respiration of cyanobacteria Koichi Yoshina (Masayuki Ohmori*)

PI-135 (JTK)

In situ cultivation technique allows recovery of native bacterial types competitive in their extreme cold environment of northern Greenland

Dawoon Jung

PI-136 Effect of the addition of pantothenate and its precursors on coenzyme A content of Escherichia coli expressing exogeneous pantothenate kinase

Yuta Ogata

PI-137 A Two Component Sensor Affect Ethanol Degradation in Acinetobacter baumannii Kuen-Sheng Hsieh

PI-138 Isocitrate lyase controls bacterial respiration under oxidative stress Sungeun AhnPI-139 (JTK)

Expression analysis of multiple alkane hydroxylases in Acinetobacter oleivorans DR1 Woojun Park

PI-140 Physiological characterization of Nitrosomonas mobilis-like strain isolated from autotrophic nitrifying granule

Myat Thandar Soe

PI-141 Physiological responses of Actinobacteria to water stress during fed-batch composting as determined by cultural, trasncriptomic, and proteomic analyses

Takashi Narihiro (Akira Hiraishi*)

PI-142 (JTK)

Uptake of fatty acids in Pseudomonas nitroreducens TX1 cells grown solely on fatty acids ester emulsifiers

Chun-Yen Lin

PI-143 Growth-promoting, dormancy and awakening of previously uncultured microorganisms Nitrospira

Chiho Murakami

PI-144 (JTK)

Identification of genes involved in the catabolism and stress response to octylphenol polyethoxylates (Triton X-100) in Pseudomonas nitroreducens TX1 by transposon mutagenesis

Po-Chun Tsai

PI-145 ( *)

PI-146

PI-147 ldhA

PI-148 ( *)

PI-149 Chloroflexus aggregans

PI-150 Anammox 20 II. Ki

PI-151 Anammox 20 I.

PI-152

PI-153 ( *)

*Corresponding author

P-22

List of posters continued

Room Session

PS Room 4 (4th floor)

Physiology/Metabolism (continued)

PI-154 Rhodopseudomonas palustris

PI-155 Dechloromonas sp.Azospira sp.

( *)

PS Room 1 (2nd floor)

Symbiosis/Communication

PJ-156 Re-organization of symbiotic protist communities after sudden community mixing through the host termite hybridization

Osamu Kitade

PJ-157 Single-cell genome analyses of three dominant bacterial species in the gut of a higher termite

Masahiro Yuki

PJ-158 Complete genome sequencing of TC1 symbiont in Trimyema ciliate using PacBio sequencerNaoya Shinzato

PJ-159 Why are homopolymeric tracts so frequent in endosymbiont genomes? Yukihiro Kinjo

PJ-160 Determination of a suitable condition for Azospirillum sp. B510 infection and colonization in rice plant and observation of the colonization

Kamrun NaherPJ-161 (JTK)

Effect of heterotrophic bacteria on growth of a marine ammonia-oxidizing archaeon Seon-Bin Choi

PJ-162 (JTK)

Differential efficiency of nitrate respiration between Bradyrhizbium diazoefficiens and Bradyrhizobium japonicum

Arthur Fernandes Siqueira

PJ-163 Molecular genetic analysis of Bradyrhizobium elkanii mutants with altered symbiotic compatibility with Vigna radiate

Hien Nguyen Phuoc

PJ-164 Characterization of transposon mutants of Bradyrhizobium elkanii USDA61 that formed effective nodule on Rj4 soybean

Muhammad Omar Faruque

PJ-165 Genetic diversity of Methylobacterium spp. on rice in Vietnam Trinh Anh Khoa Lai

PJ-166 Evolution of verrucomicrobia from ectosymbionts to endonuclear symbionts of protists in the termite gut

Yuichi Nagura

PJ-167 The double symbiotic life: plant-associated Burkholderia are also associated with bordered plant bugs (Heteroptera: Largidae)

Kazutaka Takeshita

PJ-168 Isolation and selection of cellulolytic dark septate endophytic fungi that able to promote asparagus plant growth

Surono

PJ-169 Chaetoceros tenuissimus DNA/RNA

( *)

PJ-170 Quorum sensing

PJ-171 dsDNA Heterosigma akashiwo virus

PJ-172

PJ-173

PJ-174 Veronaeopsis simplex Rhizobium sp. Y9

*Corresponding author

P-23

List of posters continued

Room Session

PS Room 1 (2nd floor)

Symbiosis/Communication (continued)

PJ-175 Eikenella corrodens

PJ-176 Paracoccus denitrificans

PJ-177

PJ-178

PJ-179 Chromobactrium violaceum

PJ-180

PJ-181

PJ-182

PJ-183

PJ-184 Rj4 3

PJ-185 Bradyrhizobium elkanii

PJ-186 nifH

PJ-187 RNA

PJ-188

Methodology

PK-189 Comparison of oligonucleotide and polynucleotide probes for enrichment of target genes from environmental metagenomes by in-solution capture hybridization

Tomohiro Tobino

PK-190 Letting microorganisms escape loneliness and grow Yuki TakagiPK-191 (TK)

Screening and characterization of biosurfactant-producing yeast Jong-Shik Kim

PK-192 (TK)

Molecular approach for detection of anaerobic ammonium-oxidizing bacteria in sludge for livestock wastewater

Sunja Cho

PK-193

PK-194 GeneFISH

PK-195

PK-196 DNA

PK-197

PK-198

P-24

List of posters continued

Room Session

PS Room 1 (2nd floor)

Interface/Biofilm

PL-199 Morphological characteristics of Shewanella biofilms formed on graphite electrodes in bioelectrochemical systems

Miho Kitayama

PL-200 (JTK)

Endogenous hydrogen peroxide increases biofilm formation by inducing exopolysaccharide production in Acinetobacter oleivorans DR1

Woojun Park

PL-201 ( *)

PL-202 ( *)

PL-203 ( *)

PL-204

PL-205

PL-206 Paenibacillus

PL-207 Nonribosomal peptide synthetase

PL-208 Lactobacillus plantarum

PL-209

PL-210

PL-211 Streptococcus mutans Biofilm

PL-212 Paracoccus denitrificans

PL-213 ( *)

PL-214

PS Room 2 (2nd floor)

Plant pathogen

PM-215 Ralfuranones secreted by Ralstonia solanacearum function as intercellular second messenger molecules during its biofilm formation

PM-216 (JTK)

Identification chemoreceptor for D-malic acid, an unnatural occurred form, in Ralstonia solanacearum

Mattana Tunchai

PM-217

The quorum sensing system with 3-hydroxymyristate as a quorum sensing signal is implicated in biofilm formation by Ralstonia solanacearum strain OE1-1, leading to its virulence

PM-218 (JTK)

Genes associated with melanin-like pigment production in Ralstonia solanacearum Seung Yeup Lee

PM-219

PM-220 ( *)

*Corresponding author

P-25

List of posters continued

Room Session

PS Room 2 (2nd floor)

Environmental hygiene

PN-221 Detection of uncultured Legionella spp. in cooling tower water samples by amoebic coculturing and quantitative PCR

Hiroaki InouePN-222 (JTK)

Verification of commercial animal probiotics from Korea using barcoded pyrosequencing Hyo Jung Lee

PN-223 (JTK)

Two Aspergillus species from soil environment associated with otitis in the same patient in Taiwan

Roland KirschnerPN-224 (JTK)

Transcriptome analysis of Agromyces luteolus with zearalenone-removal activity Yong-Hoon Jung

PN-225 Alternaria alternata ( *)

PN-226 Mycobacterium ( *)

Bioremediation PO-227 (JTK)

Biodegradation of tetrabromobisphenol-A in sludge-amended soil and microbial community change

Bea-Ven ChangPO-228 (JTK)

A comprehensive method for HAB species controlling and toxin degradation using bacteria Thi Thao Vo

PO-229 (JTK)

The effect of drought stress on the production of astaxanthin by Haematococcus pluvialis and drawing the optimum curve

Sang-Il Han

PO-230 (JTK)

Changes of bacterial and archaeal communities in glycerol-fed anaerobic digester on operation time

Sunja Cho

PO-231

PO-232 Stenotrophomonas maltophilia p- ( *)

PO-233 Bradyrhizobium elkanii USDA94 cadABCK

PO-234

PO-235

PO-236 Pseudomonas sp. NP-2

PO-237

*Corresponding author Best Poster Award Poster authors are recommended to attend their posters during the following time slot, which is scheduled for referees' review for the Best Poster Award.

authors of posters with odd numbers, Sunday, 15:20 - 16:20 authors of posters with even numbers, Monday, 15:00 - 16:00

The 5 award-winning posters will be reported at the banquet on Monday (Oct 19).

P-26

List of posters continued

Room Session

Foyer A (1st floor)

JSME Inter-high school poster session

HS-238

HS-239

HS-240

HS-241

HS-242 Observing the mysterious ecology of Hikarimo

Omiya HarukaSasaki KarenRyoma Kawasaki and Yuta KuriharaIbaraki Prefectural Hitachi First Senior High School

HS-243

HS-244

HS-245

HS-246

HS-247

HS-248

HS-249

Foyer A (1st floor) HS-238 249 JSME- 10/18() 10:20-11:20 15:00-16:20 16:50 A

P-27

HS-238

HS-241

HS-244

HS-247

PS Room 1 (2nd floor) Symbiosis/Communication PJ-156 188 Methodology PK-189 198 Interface/Biofilm PL-199 214 PS Room 2 (2nd floor) Plant pathogen PM-215 220 Environmental hygiene PN-221 226 Bioremediation PO-227 237

P-28

PS Room 3 (4th floor) Aquatic ecosystem, PA-001 025; Soil ecosystem, PB-026 045; Forest ecosystem, PC-046 050 Waste water treatment, PD-051 063; Material cycles, PE-064 071 Extreme environment, PF-072 088; Phylogeny/Taxonomy, PG-089 100 PS Room 4 (4th floor) Phylogeny/Taxonomy PG-101 104 Genome PH-105 126 Physiology/Metabolism PI-127 155

P-29

Entrance

Co-organized by

Japanese Society of Microbial Ecology (JSME) Taiwan Society of Microbial Ecology (TSME) The Microbiological Society of Korea (MSK)

JTK/JSME Organizing committee

Chair: Hiroyuki Ohta, Ibaraki University (IU), Japan JTK Board members:

Koji Hamasaki, The University of Tokyo, Japan Satoshi Hanada, National Institute of Advanced Industrial Science and Technology (AIST), Japan Seigo Amachi, Chiba University, Japan Natsuko Hamamura, Kyushu University, Japan Shir-Ly Huang, National Central University, Taiwan Jyh-Yih Leu, Fu Jen Catholic University, Taiwan Gia-Luen Guo, Institute of Nuclear Energy Research, Taiwan Woojun Park, Korea University, Korea Jong-Chan Chae, Chonbuk National University, Korea Che Ok Jeon, Chung-Ang University, Korea

JTK Executive advisors

Kazuhiro Kogure, The University of Tokyo, Japan Shiu Mei Liu, National Taiwan Ocean University, Taiwan Sang-Jin Kim, Korea Institute of Ocean Science & Technology, Korea

JSME Local organizing committee

Secretary-general and Treasurer: Shigeru Chohnan, IU

General affairs: Tomoyasu Nishizawa, IU

JSME Symposium organizing committee: Kazuhiko Narisawa, IU Naomi Sakurai, Ibaraki Prefectural University of Health Sciences (IPUHS) Masako Takashima, RIKEN BioResource Center Shigeki Yamamura, National Institute for Environmental Studies

JSME/JTK Oral/poster session organizing committee: Kaoru Kumada, Tsukuba International University Tetsushi Suyama, AIST Moriya Ohkuma, RIKEN BioResource Center Osamu Kitade, IU

Outreach: Yasurou Kurusu, IU Yoko Katayama, Tokyo University of Agriculture and Technology (TUAT) Naomi Sakurai, IPUHS Michinari Sunamura, The University of Tokyo Ayako Furutani, IU

Inter-high school poster session: Risa Nonaka, Dokkyo Medical University Osamu Kitade, IU

JSME Wakate () session: Satoshi Tamazawa, AIST; Yuya Sato, AIST; Hiroyuki Kusada, AIST; Yumi Mori, Tohoku University; Tomohiro Inaba, AIST

Other contributions: Hisao Kuroda, IU; Nobuo Sakagami, IU; Miho Kanemoto, TUAT & IU

ISME young scientist session organizers

Takashi Narihiro, AIST, Japan Hideyuki Tamaki, AIST, Japan Susumu Yoshizawa, The University of Tokyo, Japan

P-30

Student representatives

Kenshi Suzuki, Shizuoka University, Japan Yi-Lung Chen, National Taiwan Normal University, Taiwan Sungeun Ahn, Korea University, Korea

Registration fee from 1st September Banquet

Regular participant Date and Time: Oct 19, Monday, 19:00-21:00 11,000 JPY (JSME, TSME or MSK member) Site: Hotel Marroad Tsukuba 13,000 JPY (non-member) Fee:

Student From 1 September 5,000 JPY (JSME, TSME or MSK member) 7000 JPY (regular participant) 6,000 JPY (non-member) 6000 JPY (student)

Other social events

Welcome Reception (Open to all participants at Foyer B) Date: Oct 17, Saturday

City Map

Tourist Guide map is available from: http://www.city.tsuchiura.lg.jp/data/doc/1305536264_doc_26_0.pdf

Contact

Meeting office: JSMEmeeting@gmail.com - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Other reminders The abstract book is not printed but all the abstract files will be uploaded to the website by Oct 17. There is a small restaurant (seating capacity, about 30) in the venue. Because no restaurant is available near the venue, we will give information about a box lunch (Obento). Please return the name card case to the registration desk at the end of the 7th JTK/JSME2015.

P-31

Transport from Narita / Haneda to Tsuchiura (1) Narita Int. Airport >> (Airport Shuttle Bus) >> Tsuchiura JR station Bus fare, 2,600 yen

Departure time/Narita Int. Airport Arrival time/ Tsuchiura JR

station Terminal 3 Terminal 2 Terminal 1

7:35 7:40 7:45 ---> 9:40 9:00 9:05 9:10 ---> 11:05 10:30 10:35 10:40 ---> 12:35 12:45 12:50 12:55 ---> 14:50 14:25 14:30 14:35 ---> 16:30 16:10 16:15 16:20 ---> 18:15 17:15 17:20 17:25 ---> 19:20 18:40 18:45 18:50 ---> 20:45 20:05 20:10 20:15 ---> 22:10

On the Tsuchiura - Narita Airport routes, reservations may only be made on buses heading to Narita Airport. No reservations are necessary for buses leaving Narita. [Direction bound Tsuchiura] It will be released only on the day. Please purchase before boarding in the sale office below: The First Passenger Terminal North Wing, 1F Keisei counter The Second Passenger Terminal, 1F Keisei counter The Third Passenger Terminal, 1F high-speed bus ticket counter (2) Haneda Int. Airport >>(Keikyu Railway)>> Shinagawa JR station >>(JR)>> Tsuchiura JR station

Time Table Keikyu Railway JR Ueno-Tokyo Line JR Joban Line Rail rate, 410 yen Rail rate, 1,320 yen (+ 1,000 yen when using LE)*

Haneda Int. St.

Shinagawa St

Tokyo St. Ueno St.**

Limited Express (LE) Express (E) Local (L)

Tsuchiura St.

6:05 6:26 6:44 6:53 7:00 >>Hitachi 1 (LE)>> 7:41

Train services are available every 5-10 min

Train services are available every several

min by Yamanote (Y) and Keihin-tohoku (K) Lines

7:30 >>Tokiwa 51(LE)>> 8:17 8:00 >>Hitachi 3 (LE)>> 8:49 8:30 >>Tokiwa 53(LE)>> 9:16 9:30 >>Tokiwa 55(LE)>> 10:15

8:56 9:16 9:33 9:43 9:49 >>(L)>> 11:00 9:16 9:36 9:53 10:03 10:09 >>(E)>> 11:08 9:45 9:56 10:14 10:23 10:30 >>Tokiwa 57(LE)>> 11:14 10:25 10:36 10:53 11:03 11:09 >>(E)>> 12:09 10:44 10:57 11:14 11:23 11:30 >>Tokiwa 59(LE)>> 12:15

11:16 11:26 11:32 >>(L)>> 12:44 11:24 11:37 11:53 12:03 12:09 >>(E)>> 13:06 11:45 11:56 12:14 12:23 12:30 >>Tokiwa 61(LE)>> 13:12

12:16 12:26 12:32 >>(L)>> 13:44 12:25 12:36 12:53 13:03 13:09 >>(E)>> 14:06 12:44 12:57 13:14 1323 13:30 >>Tokiwa 63(LE)>> 14:12

13:16 13:26 13:32 >>(L)>> 14:44 13:24 13:37 13:53 14:03 14:09 >>(E)>> 15:06

14:14 14:23 14:30 >>Tokiwa 65(LE)>> 15:12 13:45 13:56 14:16 14:26 14:32 >>(L)>> 15:44

P-32

14:25 14:36 14:53 15:03 15:09 >>(E)>> 16:06 14:44 14:57 15:14 15:23 15:30 >>Tokiwa 67(LE)>> 16:13

15:16 15:26 15:32 >>(L)>> 16:48 15:24 15:37 15:52 16:02 16:08 >>(L)>> 17:19 15:45 15:56 16:14 16:23 16:30 >>Tokiwa 69(LE)>> 17:10

16:16 16:26 16:32 >>(L)>> 17:44 15:54 16:07 16:26 16:36 16:44 >>(L)>> 17:58

Every 5-10 min Y/K Lines 17:30 >>Tokiwa 71(LE)>> 18:16 16:44 16:57 17:14 17:25 17:33 >>(L)>> 18:44 17:14 17:27 17:44 17:53 18:00 >>Hitachi 23 (LE)>> 18:38

every 5-10 min Y/K Lines 18:15 >>Tokiwa 73(LE)>> 19:05 18:30 >>Tokiwa 75(LE)>> 19:14

18:14 18:27 18:44 18:53 19:00 >>Hitachi 25 (LE)>> 19:38 every 5-10 min Y/K Lines 19:15 >>Tokiwa 77(LE)>> 20:08

19:30 >>Tokiwa 79(LE)>> 20:15 19:14 19:27 19:44 19:53 20:00 >>Hitachi 27 (LE)>> 20:40

every 5-10 min Y/K Lines 20:15 >>Tokiwa 81(LE)>> 21:06 20:30 >>Tokiwa 83(LE)>> 21:16

20:14 20:27 20:44 20:53 21:00 >>Hitachi 29 (LE)>> 21:41 every 5-10 min Y/K Lines 21:15 >>Tokiwa 85(LE)>> 22:08

21:30 >>Tokiwa 87(LE)>> 22:15 21:14 21:27 21:44 21:53 22:00 >> Tokiwa 89(LE)>> 21:41 every 10-20 min Y/K Lines 22:15 >>Tokiwa 91(LE)>> 23:09

22:30 >>Tokiwa 93(LE)>> 23:20 22:11 22:30 22:44 22:53 23:00 >> Tokiwa 95(LE)>> 23:50

* How to purchase tickets, visit the web site: https://www.jreast.co.jp/e/ticket/index.html **Local train services are available every 20 (morning time) 30 (day time) min between Ueno station and

Tsuchiura station. Information on JR Tsuchiura station is available from: (Map) http://www.jreast.co.jp/estation/stations/1011.html (Service) https://www.jreast.co.jp/e/

Transport from Tsuchiura to Narita / Haneda (3) Tsuchiura JR station >> (Airport Shuttle Bus) >> Narita Int. Airport Bus fare, 2,600 yen

Departure time/ Tsuchiura JR station

Arrival Departure time/Narita Int. Airport Terminal 2 Terminal 1 Terminal 3

5:30 ---> 7:30 7:35 7:40 6:40 ---> 8:40 8:45 8:50 8:30 ---> 10:25 10:30 10:35 10:20 ---> 12:15 12:20 12:25 12:00 ---> 13:55 14:00 14:05 13:15 ---> 15:10 15:15 15:20 14:15 ---> 16:10 16:15 16:20 15:30 ---> 17:25 17:30 17:35 17:15 ---> 19:10 19:15 19:20

On the Tsuchiura - Narita Airport routes, reservations may only be made on buses heading to Narita Airport. No reservations are necessary for buses leaving Narita. Reservations can be made on the web site: http://kantetsu.co.jp/bus_reserve/

The followings are quoted from the web site: Please purchase a ticket at [convenience store] purchase deadline (of boarding the day before 19 o'clock) or less of a convenience store before. Seven-Eleven Lawson FamilyMart during the Circle K Sunkus Click here for details looking for tickets, so you will need a reservation number, please refrain from the advance reservation

number. refund at the convenience store over-the-counter, does not allow the handling of the contents change,

please contact the contact listed in the ticket back. (4) Tsuchiura JR station >>(JR)>> Shinagawa JR station>>(Keikyu Railway)>> Haneda Int.

Airport Time Table

JR Joban Line JR Ueno-Tokyo Line Keikyu Railway

Rail rate, 1,320 yen (+ 1,000 yen when using LE)* Rail rate, 410 yen Tsuchiura

St. Limited Express (LE)

Express (E) Local (L)

Ueno St.**

Tokyo St. Shinagawa St

Haneda Int. St.

6:06 >>Tokiwa 52(LE)>> 7:05 Train services are available every several min by

Yamanote (Y) and Keihin-tohoku (K) Lines

Train services are available every 10-15 min 6:20 >>Tokiwa 54(LE)>> 7:23

6:31 >>Tokiwa 56(LE)>> 7:34 6:40 >>Tokiwa 58(LE)>> 7:46 7:00 >>Tokiwa 60(LE)>> 8:04 7:39 >>Tokiwa 62(LE)>> 8:43 7:54 >>(L)>> 9:05 9:13 9:24 7:58 >>Hitachi 2 (LE)>> 8:55 9:03 9:13 8:14 >>(L)>> 9:22 9:28 9:38 8:19 >>Tokiwa 64(LE)>> 9:10 Y/K Lines

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8:35 >>(L)>> 9:45 9:53 10:03 8:39 >>Hitachi 4 (LE)>> 9:32 9:40 9:51 9:12 >>(L)>> 10:21 10:27 10:36 9:20 >>Tokiwa 66(LE)>> 10:04 10:11 10:21 9:28 >>(L)>> 10:42 10:49 10:59 10:00 >>(E)>> 10:58 11:04 11:13 10:25 >>Tokiwa 70(LE)>> 11:06 11:13 11:22 10:30 >>(L)>> 11:42 11:49 11:59 11:00 >>(E)>> 11:58 12:04 12:13 11:25 >>Tokiwa 72(LE)>> 12:06 12:13 12:22 11:30 >>(L)>> 12:42 12:49 12:59 12:00 >>(E)>> 12:58 13:04 13:13 12:25 >>Tokiwa 74(LE)>> 13:06 13:13 13:22 12:30 >>(L)>> 13:42 13:49 13:59 13:00 >>(E)>> 13:58 14:04 14:13 13:25 >>Tokiwa 76(LE)>> 14:06 14:13 14:22 13:30 >>(L)>> 14:42 14:49 14:59 14:00 >>(E)>> 14:58 15:04 15:13 14:25 >>Tokiwa 78(LE)>> 15:08 15:13 15:23 14:32 >>(L)>> 15:41 15:49 15:59 15:00 >>(E)>> 15:58 16:04 16:13 15:25 >>Tokiwa 80(LE)>> 16:07 16:14 16:23 15:30 >>(L)>> 16:34 16:41 16:50 16:25 >>Tokiwa 82(LE)>> 17:06 Train services are available

every several min by Yamanote (Y) and Keihin-

tohoku (K) Lines

17:25 >>Tokiwa 84(LE)>> 18:07 18:24 >>Tokiwa 86(LE)>> 19:10 19:24 >>Tokiwa 88(LE)>> 20:06 20:24 >>Tokiwa 90(LE)>> 21:06 21:24 >>Tokiwa 92(LE)>> 22:05 21:56 >>Hitachi 30 (LE)>> 22:37 22:43 22:53 23:10 23:29 22:24 >>Tokiwa 94(LE)>> 23:05 Y/K Lines 23:52 24:11

* How to purchase tickets, visit the web site: https://www.jreast.co.jp/e/ticket/index.html **Local train services are available every 20 (morning time) 30 (day time) min between Tsuchiura station

and Ueno station. Information on JR Tsuchiura station is available from: (Map) http://www.jreast.co.jp/estation/stations/1011.html (Service) https://www.jreast.co.jp/e/