Michelle Anthony

Khadija Lawrence

The Effects of Ampicillin on the Growth of E. Coli

Escherichia coli

Gram-negative Rod shapedPathogenic strains

http://www.universityofcalifornia.edu/everyday/agriculture/images/e_coli.jpg

Ampicillin

http://upload.wikimedia.org/wikipedia/commons/4/48/Ampicillin-2D-skeletal.png

The maximum growth of E. coli will be inhibited as the concentration of ampicillin is

increased.

Hypothesis

Culture (E. coli)Ampicillin 10x lower LBH2O

Components

P. fluorescens resistant

Titrations No derived conclusionLead to redesign

Experiments 1-4

P. fluorescens resistant

Titrations No derived conclusionLead to redesign

Experiments 1-4

P. fluorescens resistant

Titrations No derived conclusionLead to redesign

Experiments 1-4

No titrationsAltered amounts of ampicillin

Varied the ranges each week

Experiments 5-10

No titrationsAltered amounts of ampicillin

Varied the ranges each week Results from each week lead to

final experiment

Experiments 5-10

LB H2O E. coli Amp

1 25 224 1 0

2 25 221 1 3

3 25 219 1 5

4 25 215 1 9

5 25 212 1 12

6 25 208 1 16

7 25 204 1 20

8 25 199 1 25

* All values in table are in microL

Experimental Design

1 10 19 28 37 46 55 64 73 82 91 1001091181271361451541631721811901992082172262352442532620

0.2

0.4

0.6

0.8

1

1.2

1.4

E. coli Growth vs Increasing Amounts of Ampicillin

Time (mins)

Grow

th

1612950 3

Maximum growth was not inhibited Normal curve max: 1.088Highest amp max: 1.044

Lag phase dramatically affected after 16microL is exceeded

IC50 not determined Ampicillin affects length of lag phase

Graph consistent with scale

Conclusion derived from Results

http://www.cdc.gov/ecoli/general/http://www.foodsafety.gov/poisoning/causes/b

acteriaviruses/ecoli/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1583696/?page=4

References