Methods in Enzymology

Volume 148

Plant Cell Membranes

Lester Packer Roland Douce

CONTRIBUTORS TO VOLUME 148 xi

PREFACE XVii

VOLUMES IN SERIES XiX

Section I. Cells, Protoplasts, and Liposome s

1. Techniques of Cell Suspension Culture

RICHARD BLIGNY ANDJEAN-JACQUES LEGUAY

3

2 . Culture and Characteristics of Green Plant Cells COLIN DALTON AN DIAN MACKENZIE

1 6

3 . Preparation of Protoplasts from Plant Tissues for MtKIO NISHIMURA ,Organelle Isolation

1KUKO HARA-NISHIMURA ,AND TAKASHI AKAZAWA

27

4. Interaction of Plant Protoplast and Liposome

TosHIYUKI NAGATA

3 4

5 . Liposomes as Carriers for the Transfer and Ex- MICHEL CABOCHE AN DPression of Nucleic Acids into Higher Plant Pro- PAUL F . LURQUIN

3 9

toplast s

6 . Interspecific Transfer of Partial Nuclear Genomic OTTO SCHIEDER

45

Information by Protoplast Fusio n

Section II . Vacuoles and Tonoplast s

7 . Isolation of Mature Vacuoles of Higher Plants : GEORGE J . WAGNER

5 5

General Principles, Criteria for Purity and In-tegrity

8 . Isolation of Vacuoles and Tonoplast from Proto- ALAIN M . BOUDET AN Dplasts

GILBERT ALIBERT

74

9 . Preparation of Tonoplast Vesicles from Isolated ROBERT T . LEONARD

82

Vacuole s

10 . Biochemical and Enzymatic Components of a J . D'AUZAC, H . CHRESTIN ,Vacuolar Membrane : Tonoplast of Lutoids AND B . MARIN

87

from Hevea Latex

11 . Characterization of Tonoplast Enzyme Activities BENJAMIN JACOBY

105and Transport

12 . Preparation of Tonoplast Vesicles : Applications to EDUARDO BLUMWALD ,H + -Coupled Secondary Transport in Plant Vac- PHILIP A. REA, AN Duoles

RONALD J . POOLE

11 5

13, Purification and Characterization of the Tonoplast STEPHEN K . RANDALL AN DH + -Translocating ATPase

HEVEN SZE

12 3

14 . High-Performance Liquid Chromatography for Si- M . HILL, A . DuPAIx ,multaneous Kinetic Measurements of Adenine P . VOLFIN, A . KURKDJIAN ,Nucleotides in Isolated Vacuoles

AND B . ARRIO

13 2

Section III . Plastids

15 . Isolation of Intact Chloroplasts : General Princi- DAVID A . WALKER ,pies and Criteria of Integrity

ZORAN G . CEROVIC, ANDSIMON P . ROBINSON

145

16. Isolation of Plastids in Density Gradients of Per- CARL A . PRICE ,coil and Other Silica Sols

JOHN C . CUSHMAN ,LETICIA R .MENDIO LA-MO RGENTHALER,AND ELLEN M . REARDON

157

17. Purification of Chloroplasts Using Silica Sols

K . W . Joy AND W . R . MILLS 179

18 . Separation of Chloroplasts and Cytosol from Pro- SIMON P . ROBINSON

188toplasts

19 . Use of Thermolysin to Probe the Cytosolic Sur- JACQUES JOYARD ,face of the Outer Envelope Membrane from ALBERT-JEAN DORNE ,

Plastids

AND ROLAND DOUCE

195

20. Characterization of Plastid Polypeptides from the JACQUES JOYARD ,Outer and Inner Envelope Membranes

MARYSE A . BLOCK ,JACQUES COV$S ,

CLAUDE ALBAN ,AND ROLAND DOUCE

20 6

21 . Isolation of Amyloplasts from Suspension Cul- Tom AP REES AN Dtures of Soybean

FRASER D . MACDONALD

21 8

22 . Isolation of Amyloplasts from Developing Endo- JACK C . SHANNON ,sperm of Maize (Zea mays L .)

EDGARDO ECHEVERRIA ,AND CHARLES BOYER

22 6

23 . Isolation of Plastids from Buds of Cauliflower ETIENNE-PASCAL JOURNET

234(Brassica oleracea L . )

24 . Isolation of Membranous Chromoplasts from Daf- Bono LIEDVOGEL

24 1fodil Flowers

25 . Structure and Function of the Inner Membrane SATORU MURAKAMI

246Systems in Etioplasts

AKERLUND Isolation Procedures for Inside-Out Thylakoid HANS-ERIK AKERLUND AN DVesicles

BERTIL ANDERSSON

252

27. Characterization of Chloroplast Cytochromes

DEREK S . BENDALL ANDSTEPHEN A . ROLFE

259

28 . Cell-Free Reconstitution of Protein Transport into MICHAEL L . MISHKIND ,Chloroplasts

KAREN L . GREER, AN DGREGORY W . SCHMIDT

274

29. Polar Lipids of Chloroplast Membranes

DAVID J . CHAPMAN ANDJAMES BARBER

294

30 . Isolation and Reconstitution of Thylakoid Lipids SALLIE G . SPRAGUE ANDL . ANDREW STAEHELIN

31 9

31 . Long-Chain Fatty Acid Synthesis and Utilization GRATTAN ROUGHAN

327by Isolated Chloroplast s

32 . Phosphatidylglycerol Synthesis in Chloroplast J . BRIAN MUDD ,Membranes

JAEN E . ANDREWS ,AND S . A . SPARACE

33 8

33 . Galactolipid Biosynthesis in Chloroplast Mem- NORA W . LEM AN Dbranes

JOHN P . WILLIAMS

346

34 . Chlorophylls and Carotenoids : Pigments of Photo- HARTMUT K . LICHTENTHALER 350synthetic Biomembrane s

35 . a-Tocopherol and Plastoquinone Synthesis in JÜRGEN SOLL

383Chloroplast Membrane s

36 . Solubilization and Reconstitution of Caroteno- P. BEYER

392genic Enzymes from Daffodil ChromoplastMembranes Using 3-[(3-Cholamidopropyl)di-methylammonio]- 1-propane Sulfonate

Section IV. Mitochondria

37. Isolation of Plant Mitochondria : General Princi- ROLAND DOUCE ,pies and Criteria of Integrity

JACQUES BOURGUIGNON ,RENAUD BROUQUISSE ,AND MICHEL NEUBURGER

40 3

38 . Purification of Plant Mitochondria on Silica Sol ANTHONY L . MOORE ANDGradients

MICHAEL O . PROUDLOVE

41 5

39 . Isolation of Mitochondria from Leaves of C 3 , C4 , GERALD E . EDWARDS AN Dand Crassulacean Acid Metabolism Plants

PER GARDESTROM

421

40 . Separation of Spinach Leaf Mitochondria accord- PER GARDESTRÖM AN Ding to Surface Properties : Partition in Aqueous INGEMAR ERICSON

434Polymer Two-Phase Systems

41. Isolation of Submitochondrial Particles with Dif- IAN M . MOLLER ,ferent Polarities

ANNIKA C . LIDEN ,INGEMAR ERICSON, AN DPER GARDESTRÖM

442

42 . Isolation of the Outer Membrane of Plant Mito- CARMEN A . MANNELLA

45 3chondria

43 . Characterization of Channels Isolated from Plant MARCO COLOMBINI

46 5Mitochondria

44. Phosphoglycerides of Mitochondrial Membranes JOHN L . HARWOOD

47 5

45 . Ubiquinone Biosynthesis in Plant Mitochondria FRIEDHELM LÜTKE-BRINKHAU SAND HANS KLEINIG

48 6

46 . Purification of Complexes II and IV from Plant MASAYOSHI MAESHIMA ,Mitochondria

TSUKAHO HATTORI, ANDTADASHI ASAHI

49 1

Section V. Peroxisomes and Glyoxysomes

47. Isolation of Glyoxysomes and Purification of Gly- EUGENE L . VIGIL ,oxysomal Membranes

TUNG K . FANG, AN DROBERT P . DONALDSON

505

48. Peroxisomes and Fatly Acid Degradation

BERNT GERHARDT

51 6

49 . Proteins and Phospholipids of Glyoxysomal Mem- HARRY BEEVERS AN Dbranes from Castor Bean

ELMA GONZALEZ

52 6

Section VI . Nuclei, Endoplasmic Reticulum, and Plasma Membrane

50 . Isolation of Nuclei from Soybean Suspension PAUL KEIM

53 5Cultures

51 . Isolation of the Plasma Membrane : Membrane DONALD P . BRISKIN ,Markers and General Principles

ROBERT T . LEONARD, AN DTHOMAS K . HODGES

542

52 . Preparation of High-Purity Plasma Membranes

CHRISTER LARSSON ,SUSANNE WIDELL ,AND PER KJELLBOM

558

53 . Possible Approaches to Surface Labeling of the J . L . HALL

568Plasma Membrane

54 . Isolation of Endoplasmic Reticulum : General J . MICHAEL LORD

57 6Principles, Enzymatic Markers, and Endoplas-mic Reticulum-Bound Polysome s

55 . Phosphoglyceride Synthesis in Endoplasmic Re- THOMAS S . MOORE, JR .

58 5ticulu m

Section VII . General Physical and Biochemical Methods

56. Electron Microscopy of Plant Cell Membranes

JEAN-PIERRE CARDE

599

57 . Two-Dimensional Electrophoresis in the Analysis R . REMY AN Dand Preparation of Cell Organelle Polypeptides F . AMBARD-BRETTEVILLE

623

58 . Plant Membrane Sterols : Isolation, Identification, MARIE-ANDREE HARTMANN

and Biosynthesis

AND PIERRE BENVENISTE

63 2

59 . Separation of Molecular Species of Plant Glyco- J . KESSELMEIER AN Dlipids and Phospholipids by High-Performance E . HEINZ

650Liquid Chromatograph y

60 . Movement of Phospholipids between Membranes : JEAN-CLAUDE KADER ,

Purification of a Phospholipid Transfer Protein CHANTAL VERGNOLLE ,from Spinach Leaf

AND PAUL MAZLIAK

66 1

61 . Pesticides and Lipid Synthesis in Plant Mem- C . ANDING

667brane s

62 . Rapid Filtration Technique for Metabolite Fluxes YVES DUPONT AN Dacross Cell Organelles

MARIE-JOSE° MOUTIN

67 5

63 . Application of Nuclear Magnetic Resonance R . G . RATCLIFFE

68 3Methods to Plant Tissue s

AUTHOR INDEX 70 1

SUBJECT INDEX 729