MC-406

Oleic acid, [1-14C]-

Lot 742-084-055-A-20120828-SBA

A) All chromatograms were run using the HPLC method described on the Product Data Sheet.

Concentrations and volumes:

Standard solution concentration was unknown.

Oleic acid, [1-14C]- concentration was 0.1 mCi/ml. Volume of standard alone injection was 2.0 µl.

Volume of Oleic acid, [1-14C]- alone injection was 0.5 µl.

Co-injection solution consisted of 0.75 µl Oleic acid, [1-14C]- + 4.0 µl standard.

Volume of co-injection was 4.75 µl.

Volume of blank injection was 2.0 µl.

B) Mass spectrometry – Negative mode

C) NMR

MC-406Oleic acid, [1-14C]-

Lot 742-084-055-A-20120828-SBA

Chromatogram Info:

File Name : inth3554 File Created : 9/20/2013 1:34:54 PM

Origin : Acquired, Acquisition started 9/20/2013 12:54:53 PM Acquired Date : 9/20/2013 1:34:52 PM

Project : Test By : Administrator

Method : Unit17_40_min_run By : Administrator

Description : Radiochemical trace of 14C material alone

Created : 8/9/2007 8:59 AM Modified : 9/26/2013 3:13 PM

Column : Detection : Radiochemical

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[mV]

Volta

ge

0

200

400

600

800

23.2

23

.9

int h3554 - Unit 17 Radio

9/26/2013 3:15 PM Page 1 of 2Chromatogram c:\Clarity\Test\Data\inth3554.PRM

Result Table (Uncal - inth3554 - Unit 17 Radio)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

1 23.17 99.72 15332.29 796.69 98.79 0.00

2 23.93 0.28 42.31 9.73 1.21 309666.58

Total 100.00 15374.61 806.42 100.00

9/26/2013 3:15 PM Page 2 of 2Chromatogram c:\Clarity\Test\Data\inth3554.PRM

MC-406Oleic acid, [1-14C]-

Lot 742-084-055-A-20120828-SBA

Chromatogram Info:

File Name : inth3554 File Created : 9/20/2013 1:34:54 PM

Origin : Acquired, Acquisition started 9/20/2013 12:54:53 PM Acquired Date : 9/20/2013 1:34:52 PM

Project : Test By : Administrator

Method : Unit17_40_min_run By : Administrator

Description : UV trace of 14C material alone

Created : 8/9/2007 8:59 AM Modified : 9/26/2013 3:17 PM

Column : Detection : UV 210nm

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[mV]

Volta

ge

-2

-1

0

1

2

3

23.0

int h3554 - Unit 17 UV

9/26/2013 3:17 PM Page 1 of 2Chromatogram c:\Clarity\Test\Data\inth3554.PRM

Result Table (Uncal - inth3554 - Unit 17 UV)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

1 22.95 100.00 6.33 0.67 100.00 133242.78

Total 100.00 6.33 0.67 100.00

9/26/2013 3:17 PM Page 2 of 2Chromatogram c:\Clarity\Test\Data\inth3554.PRM

MC-406Oleic acid, [1-14C]-

Lot 742-084-055-A-20120828-SBA

Chromatogram Info:

File Name : inth3555 File Created : 9/20/2013 2:53:56 PM

Origin : Acquired, Acquisition started 9/20/2013 2:13:53 PM Acquired Date : 9/20/2013 2:53:52 PM

Project : Test By : Administrator

Method : Unit17_40_min_run By : Administrator

Description : UV trace of standard alone

Created : 8/9/2007 8:59 AM Modified : 9/26/2013 3:18 PM

Column : Detection : UV 210nm

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[mV]

Volta

ge

0

50

100

150

10.2

10

.8

11.5

12

.2

22.8

int h3555 - Unit 17 UV

9/26/2013 3:19 PM Page 1 of 2Chromatogram c:\Clarity\Test\Data\inth3555.PRM

Result Table (Uncal - inth3555 - Unit 17 UV)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

1 10.15 0.61 7.53 0.77 0.63 27368.32

2 10.75 1.09 13.44 1.31 1.08 24114.21

3 11.48 2.18 26.98 1.50 1.24 7212.49

4 12.21 0.81 10.07 0.96 0.79 29896.35

5 22.76 95.31 1177.86 117.08 96.26 99460.92

Total 100.00 1235.89 121.63 100.00

9/26/2013 3:19 PM Page 2 of 2Chromatogram c:\Clarity\Test\Data\inth3555.PRM

MC-406Oleic acid, [1-14C]-

Lot 742-084-055-A-20120828-SBA

Chromatogram Info:

File Name : inth3556 File Created : 9/20/2013 4:35:11 PM

Origin : Acquired, Acquisition started 9/20/2013 3:55:09 PM Acquired Date : 9/20/2013 4:35:08 PM

Project : Test By : Administrator

Method : Unit17_40_min_run By : Administrator

Description : Radiochemical trace of 14C material co-injected with standard

Created : 8/9/2007 8:59 AM Modified : 9/26/2013 3:19 PM

Column : Detection : Radiochemical

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

0 10 20 30 40

[V]

Volta

ge

0.0

0.2

0.4

0.6

0.8

1.0

1.2

23.2

24

.0

24.1

int h3556 - Unit 17 Radio

9/26/2013 3:21 PM Page 1 of 2Chromatogram c:\Clarity\Test\Data\inth3556.PRM

Result Table (Uncal - inth3556 - Unit 17 Radio)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

1 23.19 99.71 19887.16 990.53 98.63 0.00

2 23.97 0.14 26.95 5.17 0.51 500856.75

3 24.10 0.15 30.52 8.64 0.86 853379.54

Total 100.00 19944.63 1004.34 100.00

9/26/2013 3:21 PM Page 2 of 2Chromatogram c:\Clarity\Test\Data\inth3556.PRM

MC-406Oleic acid, [1-14C]-

Lot 742-084-055-A-20120828-SBA

Chromatogram Info:

File Name : inth3556 File Created : 9/20/2013 4:35:11 PM

Origin : Acquired, Acquisition started 9/20/2013 3:55:09 PM Acquired Date : 9/20/2013 4:35:08 PM

Project : Test By : Administrator

Method : Unit17_40_min_run By : Administrator

Description : UV trace of 14C material co-injected with standard

Created : 8/9/2007 8:59 AM Modified : 9/26/2013 3:21 PM

Column : Detection : UV 210nm

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[mV]

Volta

ge

0

50

100

150

200

10.1

10

.4

11.1

11

.8

12.5

19.7

23.0

28.8

int h3556 - Unit 17 UV

9/26/2013 3:22 PM Page 1 of 2Chromatogram c:\Clarity\Test\Data\inth3556.PRM

Result Table (Uncal - inth3556 - Unit 17 UV)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

1 10.08 0.15 3.20 0.40 0.19 41747.48

2 10.45 0.67 14.61 1.38 0.66 24632.61

3 11.07 1.10 24.04 2.36 1.12 25330.70

4 11.81 2.40 52.20 2.81 1.34 7173.27

5 12.53 0.73 15.90 1.60 0.76 36832.75

6 19.72 0.09 2.00 0.24 0.11 135305.36

7 22.96 94.59 2059.20 200.86 95.60 99136.14

8 28.79 0.27 5.78 0.47 0.22 110666.15

Total 100.00 2176.93 210.11 100.00

9/26/2013 3:22 PM Page 2 of 2Chromatogram c:\Clarity\Test\Data\inth3556.PRM

MC-406Oleic acid, [1-14C]-

Lot 742-084-055-A-20120828-SBA

Chromatogram Info:

File Name : inth3553 File Created : 9/20/2013 11:38:59 AM

Origin : Acquired, Acquisition started 9/20/2013 10:58:57 AM Acquired Date : 9/20/2013 11:38:56 AM

Project : Test By : Administrator

Method : Unit17_40_min_run By : Administrator

Description : UV trace of blank injection

Created : 8/9/2007 8:59 AM Modified : 9/26/2013 3:23 PM

Column : Detection : UV 210nm

Mobile Phase : Temperature :

Flow Rate : Pressure :

Note :

[min.]Time

10 20 30 40

[mV]

Volta

ge

-10

0

10

20

int h3553 - Unit 17 UV

9/26/2013 3:24 PM Page 1 of 2Chromatogram c:\Clarity\Test\Data\inth3553.PRM

Result Table (Uncal - inth3553 - Unit 17 UV)

CompoundName

Reten. Time[min]

Area[%]

Area[mV.s]

Height[mV]

Height[%]

Efficiency[th.pl]

No peak to report

9/26/2013 3:24 PM Page 2 of 2Chromatogram c:\Clarity\Test\Data\inth3553.PRM

MC406-20120828-SBA-ms-2 _130926105737 9/26/2013 10:57:37 AM C:\xcalibur\data\MC406-20120828-SBA-ms-

RT: 0.00 - 1.99

0.0 0.5 1.0 1.5

Time (min)

0

5

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

95

100

Relative Abundance

0.35 0.50

0.76

0.901.04 1.95

1.790.23 1.25

1.281.56

1.15 1.50

1.48

NL:5.13E9

TIC MS MC406-20120828-SBA-ms-2 _130926105737

MC406-20120828-SBA-ms-2 _130926105737#T: - c NSI Full ms [50.00-600.00]

m/z Intensity Relativem/z Intensity Relative

280.77 2254332.9 0.66 281.31 22454.1 0.01 281.94 45801403.2 13.46 282.90 7966626.6 2.34 283.92 340248494.2 100.00 284.95 25126912.0 7.38 285.97 9560107.0 2.81 286.68 1225528.4 0.36 287.68 2584671.6 0.76 288.83 1439821.4 0.42 289.92 2266251.4 0.67 290.61 2331259.9 0.69 291.74 3904948.0 1.15 292.56 1320744.0 0.39 293.20 981643.6 0.29 294.03 13057194.7 3.84 294.85 2611601.2 0.77 295.82 14766990.3 4.34 296.34 6.8 0.00 296.85 4341474.2 1.28 297.99 109443475.9 32.17 298.83 16414153.0 4.82 299.78 78637129.6 23.11 300.80 14485124.5 4.26 301.34 123551.1 0.04 301.91 6583331.8 1.93

MC406-20120828-SBA-ms-2 _130926105737 #1-191 RT: 0.01-1.99 AV: 191 NL:T: - c NSI Full ms [50.00-600.00]

100 200 300 400 500 600

m/z

0

5

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

95

100

Relative Abundance

283.92

589.82

297.99

311.72

325.76183.62

339.66567.47

480.29352.03173.82 211.6797.47

MC406-20120828-SBA-ms-2 _130926105737 #1 RT: 0.01 AV: 1 NL: 2.04E8T: - c NSI Full ms [50.00-600.00]

265 270 275 280 285 290 295

m/z

0

10

20

30

40

50

60

70

80

90

100

110

120

130

140

150

160

Relative Abundance

283.83

284.84

293.87267.80282.03

270.64 285.92278.91

98

76

54

32

1pp

m

0.901

1.2871.327

1.645

2.032

2.3402.355

5.366

2.99

20.00

1.91

3.79

1.90

1.91

PC 1.00

GB 0

LB 0.00 Hz

SSB 0

WDW no

SF 400.1300000 MHz

SI 32768

SFO1 400.1324710 MHz

PL1W 10.75174427 W

PL1 −1.00 dB

P1 14.00 usec

NUC1 1H

======== CHANNEL f1 ========

TD0 1

D1 1.00000000 sec

TE 294.3 K

DE 6.50 usec

DW 60.400 usec

RG 4

AQ 3.9584243 sec

FIDRES 0.126314 Hz

SWH 8278.146 Hz

DS 2

NS 256

SOLVENT CDCl3

TD 65536

PULPROG zg30

PROBHD 5 mm PABBO BB−

INSTRUM spect

Time 12.37

Date_ 20130925

PROCNO 1

EXPNO 2

NAME MC406−20120828−SBA

Batch 20120828−SBA

MC406 1H NMR in CDCl3