MALDI Mass Spectrometer

MALDI-7090

MO363

2

A New Dimensionin MALDI TOF-TOF Design.. .

. . .the MALDI-7090™

Unparalleled MS/MS resolution

High mass accuracy

True 2 kHz acquisition speed in MS and MS/MS mode

Ultra fast solid-state UV laser

Low- and high-energy fragmentation

Integrated 10 plate loader

Wide-bore ion optics

In-built security and auditing

Multi-user software environment

Unique UV laser source cleaning

3

The MultiPlex™ platform has been designedas a multi-user walk-up system offeringunparalleled versati l ity and efficiency

TrueClean™• Fully automated UV laser-based source cleaning

• Novel patented Shimadzu technology

• Software-driven scanning reflective source optics to directthe laser during source cleaning

• Highly efficient, rapid cleaning without the need for sourceremoval or venting

• Planned source cleaning can be scheduled in a fullyautomated way

MultiPlex maximizes the efficient use of the MALDI-7090

through the combination of the integrated 10 plate loader, the

2 kHz ultra fast UV laser and the MALDI Solutions™ multi-user

environment software.

MultiPlex enables both manual investigation and fully

automated high-throughput analyses accommodating all types

of experiments from single spot investigation to even the

longest multi-plate LC-MALDI separations. Furthermore, it is

also compatible with the FlexiMass-SR™ steel reusable and

FlexiMass-DS™ disposable polymeric microscope slide format

MALDI targets.

The MALDI-7090 is an ideal platform for individual laboratories

and core facilities alike.

MultiPlex expertly manages the MALDI-7090 workflow.

Experimental set-up, data processing and results review is now

possible from any workstation linked to the instrument network

thus creating a highly efficient and versatile multi-user interface.

The realtime experiment queue within MultiPlex streamlines

automated analyses such as LC-MALDI by assigning specific

plate locations within the loader and monitoring progress of the

submitted experiments. Plates can be loaded and unloaded from

the 10 plate loader ready for analysis even while the instrument

is running another experiment. The loader chamber also has the

option of being held at standard atmosphere or vented to

nitrogen to protect more sensitive samples.

Designed for maximum reliability, MultiPlex offers the ultimate

flexibility in both high-throughput and multi-user environments.

Wide-bore Optics• Optimized focussing of the ion

beam using increased diameterextraction optics

• Minimized risk of sourcecontamination over time

• Reduced need for source cleaningand maintenance

Proprietary ultrafast laser

• 2 kHz ultra fast solid-state UVlaser in MS and MS/MS mode

• 355 nm wavelength compatiblewith a variety of matrixcompounds and samples

• Variable beam focus from 10 µmto >100 µm

• Reliable with long lifetime

• Innovative Shimadzu technology

HD Sample Viewing • State-of-the-art high-definition

viewing optics, with crystal clearimage

• High quality image of the MALDIsample spot

• 10 µm resolution sample viewing

• Full HD colour picture (1080p)with long-life LED illumination

• Software-controlled variablefocus, adapted to viewingdifferent surfaces

Before

After

Example of a small area cleaned using TrueClean

A source design l ike no other

MALDI-7090A New Dimension in MALDI TOF-TOF Design

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Hyper-MS2™ – a new stancombining core technologies pat

%In

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01190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300

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wa12

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y12

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PPPPPPPPPPPPPPR

ASDF™ – unparalleledMALDI TOF-TOFion resolution

ASDF – Axial Spatial Distribution Focussing is a patented

Shimadzu technology that enables unparalleled resolution

in MS/MS mode. Through correction of the axial spatial

distribution of the ions generated, mass resolution is

significantly increased and becomes essentially

independent of the laser power and sample topography.

With ASDF, the MALDI-7090 can achieve mass resolution

in MS/MS of 10 000 FWHM – unobtainable through

pulsed extraction alone. MS/MS fragmentation in the MALDI-7090 is true high-energy

CID (HE-CID).

The ions enter the CID cell with full 20 keV extraction energy.

Furthermore, collisions with helium rather than heavier gases

(air or argon) result in the most efficient generation of ions

only associated with HE-CID (e.g. side chain fragmentation).

MS/MS fragmentation is further enriched by retaining all of

the metastable decay ions through the reflectron analyzer,

maximizing the amount of information extracted from each

sample.

All of these features provide the MALDI-7090 with the

highest possible efficiency for metastable and high-energy

CID generation and detection.

High- and low-energyfragmentation

5MALDI-7090

A New Dimension in MALDI TOF-TOF Design

dard in MS/MS acquisition,ented and exclusive to Shimadzu

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m/z860 870 880 890 900 910 920 930 940 950 960 970 980

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m/z1030 1032 1034 1036 1038 1040 1042 1044 1046 1048 1050 1052 1054 1056 1058 1060 1062 1064 1066 1068 1070

MS/MSlow resolution ion gatepeptide A + peptide B

MS/MS high resolution ion gate

peptide A only

y7 peptide A

(b-H2O)7 peptide A

b7 peptide A

y7 peptide A

(b-H2O)7 peptide A

b7 peptide A

y7 peptide B

(b-H2O)7 peptide B

b7 peptide B

peptide A

peptide B

The MALDI-7090 is equipped with a dual wire

Bradbury-Nielsen high resolution precursor

ion gate.

Compounds of similar nominal mass may

produce fragment ion spectra that contain

ions from both precursors. However the high

resolution ion gate allows the individual

gating of species close in nominal mass thus

producing distinct fragment ion spectra.

High resolution ion gate

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MALDI-7090 ultimate performance

NTPpSQHDHpSIQHSPER

(M+H)+Neutral loss

of H3PO4 (98 Da)Neutral loss

of H3PO4 (98 Da)

1780 1800 1820 1840 1860 1880 1900 1920 1940 1960 1980 2000 2020

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High-energy CIDfragment ions

Hyper-MS2 – post-translational modification analysis

High-energy CID – lipid analysis

7MALDI-7090

A New Dimension in MALDI TOF-TOF Design

Coupling the MALDI-7090 with MALDI Solutions provides a stable,high-throughpu t multi-user environment which is ideally placed tomeet the demands of today’s modern high-tech laboratory

MALDI Solutions™ – a software suite delivering more forthe multi-user environment

• Define, acquire and view experiments from the MALDI-7090

• Create experiments and review results using MALDI Solutions on anycomputer on the user network

• Collaborate and share results in completely new ways

• Full data export to industry standards – imzML, mzML

• Manual acquisition mode for full control of the instrument or fullautomation using the integrated 10 plate loader

• Administrator-definable user-specific roles from simpleacquisition to full control with method development,acquisition and processing

• Centralized secure database for data storage(Microsoft® SQL server)

• User-specific access / restriction to experiments and results

• High level of security, essential in multi-user environments

• Full audit trail

• Provides the level of control required in regulatoryenvironments

• Allows 21 CFR part 11 compliance

Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation and its affiliates. In this publication, those namesand logos may be used without trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities claiming the marks and names or their products. Shimadzu Corporation disclaimsany proprietary interest in trademarks and trade names other than its own.

For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are subject to change without notice. Shimadzu does not assume any responsibility or liabilityfor any damage, whether direct or indirect, relating to, or arising out of the use of this publication.

MA

LDI-7090

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MO363 v2 May 2013

Founded in 1875, Shimadzu Corporation, a leader in thedevelopment of advanced technologies, has adistinguished history of innovation built on thefoundation of contributing to society through scienceand technology. We maintain a global network of sales,service, technical support and applications centres on sixcontinents, and have established long-term relationshipswith a host of highly trained distributors located in over100 countries. For information about Shimadzu, and tocontact your local office, please visit our web site atwww.shimadzu.com

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