making stem specimen we will look at each one of these methods in turn

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EM C ourse – M aking ofThin Film s ProfessorR odney H erring

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EM Course – Making of Thin Films

Professor Rodney Herring

Making STEM Specimen

We will look at each one of these methods in turn.

Making STEM Specimen – Dispersion Method

Making STEM Specimen – Microtomy Method

Making S(T)EM Specimen – Focus Ion Beam (FIB), Ion Mill, Plasma/UV Cleaner Method

Making STEM Specimen – Focus Ion Beam (FIB) Method

Making STEM Specimen – Focus Ion Beam (FIB) Method

Making STEM Specimen – Focus Ion Beam (FIB) Method

Making a Thin Cross-sectional Specimen by FIB

The FIB can weld any two pieces of material together at the nanoscale

Welding specimen to 33mm Cu grid

Limited Micro/Nano Machining

MEMS

Lab on a chip

Viewing of Cell Cluster

Cell Tissue (No Fixation)

Samples: Tobacco Flower Petals Courtesy: Kim Findlay, John Innes Centre, Norwich, UK

Tobacco flower petals

Cell Tissue (No Fixation)

Samples: Tobacco Flower Petals Courtesy: Kim Findlay, John Innes Centre, Norwich, UK

Tobacco flower petals

Metal deposition

Cell Tissue (No Fixation)

Samples: Tobacco Flower Petals Courtesy: Kim Findlay, John Innes Centre, Norwich, UK

Tobacco flower petals

Features never before seen were found in these specimens.

Making STEM Specimen – Focus Ion Beam (FIB) Method