Macaw #315, female

GGA1AMA1q,4,9q

Chicken control

GGA2AMA2

Chicken control

Red – GGA11 BAC pool

GGA11

GGA11

GGA11

GGA11

GGA11 – AMAZ!!!

GGA3AMA3

Chicken control

Chicken control

Red – GGA11 BAC pool

GGA4AMA1p, 8q

GGA11

GGA11

GGA11 – AMAZ!!!

GGA5AMA5q

Red: BAC P1H3 on GGA5No signal on macaw

Chicken control

GGA6AMA6 segments

Chicken controlRed: FZF – GGA6

GGA7AMA6q

Chicken controlRed: FZF – GGA6

6

6

7

7

CHICKEN CONTROLPaint for GGA6Paint for GGA7BAC clone for GGA6

Macaw-GGA6 paint and BAC

Macaw-GGA7 paint and BAC

Macaw-GGA6 and 7 paints and BAC

7

7

6

6

AMA6 GGA6 paint GGA7 paintGGA6 and GGA7 areSyntenic in macaw

Chicken GGA6 BAC clone(CHY4 gene) gives clearsignal in macaw!!!

GGA8AMA7p,7qter and micros

Chicken control

Chicken control

GGA9AMA7qmid

GGAZAMAZ

W

Chicken control (female)

W

Male- Erica Female-#315

GGAWNo signalChicken control (female)

No signal on macaw W chromosome – similar situation is with mammalian Ywhich , as a rule, does not give Zoo-FISH signal across diverged species.

5.8S, 18S, 28S rDNA

Chicken control – 1 NOR

3 pairs of microchromosomes with NORs(Chicken and California condor haveonly one NOR chromosome)

Telomeres

Macaw has telomeres at chromosome ends only -no interstitial telomeric sites.Prominent number ofTelomeric sequences are in microchromosomes

Macaw #315

W

W

Z

Z