living cerebral endothelial cells
DESCRIPTION
Living cerebral endothelial cells. György Váró. Lamorlay e, May 2011. Institute of Biophysics, Biological Research Center Szeged, Hungary. Mannitol tre a tment of the cerebral endothelial cells. Blood-brain barrier:. Mannitol:. HO - CH 2 – (CHOH) 4 – CH 2 - OH. After treatment. - PowerPoint PPT PresentationTRANSCRIPT
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György Váró
Institute of Biophysics, Biological Research CenterSzeged, Hungary
Lamorlaye, May 2011
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Mannitol treatment of the cerebral endothelial cells
Blood-brain barrier:
Mannitol:
HO - CH2 – (CHOH)4 – CH2 - OH
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Before treatment After treatment
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Before treatment
After treatment
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0.0
0.5
1.0
1.5
2.0
2.5
Cell region
Hei
ght
(µm
)
Control
Mannitol
Over the nucleus Out of nucleus
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8 kPa
0.9 kPa
0
0.2
0.4
0.6
200 0-200-400-600
forc
e (n
N)
distance (nm)
SM C
A
indentation (nm)
forc
e (n
N)
0
0.2
0.4
0.6
6004002000
MC
B
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Z. Bálint, I.a. Krizbai, I. Wilhelm, A.E. Farkas, Á. Párducz,Z. Szegletes and G. Váró (2007)
Changes induced by hyperosmotic mannitol in cerebral endothelialcells: an atomic force microscopic study.
Eur. Biophys. J. 36. 113-120
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Effect of the calcium starvation on the cells:
Healthy endothelial cells-Ca2+
Ca starved cells
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Ca starved cells
+Ca2+
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I. Wilhelm, A.E. Farkas, P. Nagyőszi, G. Váró, Z. Bálint, G.A. Végh,P.O. Couraud, I.A. Romero, B. Weksler and I.A. Krizbai (2007)
Regulation of cerebral endothelial cell morphology by extracellular calcium.
Phys. Med. Biol. 52. 6261-6274
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Petri Dish
8 µm Filter
AFM cantilever
Matrigel
RBEC LayerA2058
Transmigration of the melanoma cells
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A2058 on RBEC layer
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3
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AFM cantilever tip on hard surface
Petri dish
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AFM cantilever tip on hard surface
dwell time
load
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AFM cantilever tip on living cells
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AFM cantilever tip on living cells
Stress relaxation
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AFM tipless cantilever with melanoma cell on living cells
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AFM tipless cantilever with melanoma cell on living cells
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AFM tipless cantilever with melanoma cell on living cells
AFM tipless cantilever with melanoma cell on fixed cells
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Adhesion force: Melanoma cell on
hard surface
Melanoma cell onliving cell layer
Melanoma cell onfixed cell layer
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Stress relaxationforce
Melanoma cell on hard surface
Melanoma cell onliving cell layer
Melanoma cell onfixed cell layer
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Adhesion force properties:
Numbers of jumps
Size of the jumps
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Living cells
Fixed cells
Size of the jumps
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Stress relaxationfast exp.
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Stress relaxationslow exp.
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