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LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

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Page 1: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

LIVE INTERACTIVE LEARNING @ YOUR DESKTOP

Tuesday, January 8, 2008

NSDL/NSTA Web Seminar:

Discover Microbial Worlds

Page 2: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

http://nsdl.org

Today’s NSDL Experts:

http://www.nsta.org

Dr. Seth BordensteinEvolutionary Geneticist, Marine Biological Laboratory

Sarah BordensteinMarine Biological Laboratory Education and Outreach Coordinator

Page 3: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

http://nsdl.org

Page 4: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

http://nsdl.org

• International center for research, education, & training in biology

• Oldest private marine laboratory (1888)

• 51 Nobel Laureates• Microbial diversity,

evolution, ecology, and genomics

Page 5: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

True or False?

Most microbes cause disease.

True False

http://nsdl.org

Stamp your answer

Page 6: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds
Page 7: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds
Page 8: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

Search Term “Symbiosis”

http://nsdl.org

Page 9: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

http://nsdl.org

Page 10: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

http://nsdl.org

Page 11: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

How many bacteria are in one gram of soil?

A. 100

B. 100,000

C. 1,000,000

D. 10,000,000

http://nsdl.org

Page 12: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds
Page 13: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds
Page 14: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

When did bacteria evolve?

A. 130,000 years ago

B. 610 million years ago

C. 2 billion years ago

D. 3.8 billion years ago

http://nsdl.org

Page 15: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

EOL VIDEOwww.eol.org

Page 16: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds
Page 17: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

Evolutionary Innovations Through Animal-Bacteria Endosymbioses

• Eukaryotic cell (fusion of single celled organisms)

• Eukaryotic organelles (chloroplasts, mitochondria)

• Use of many nutrient-limited niches by animal lineages

http://nsdl.org

Page 18: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

SYMBIOSIS QUIZSYMBIOSIS QUIZ

What % of the cells in your body are human?

10% 30%

60% 90%

http://nsdl.org

Stamp your answer

Page 19: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

Insects are excellent model systemsInsects are excellent model systems

Present for 350 MY

Comprise 85% of all animal species

20% harbor bacteria called Wolbachia

Up to 6 million species are infected

YOU ARE OUR BIGGEST ASSET!http://nsdl.org

Page 20: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

Discover the Microbes Within: The Wolbachia Project

Discover the Microbes Within: The Wolbachia Project

Wolbachia symbionts

Nuclei of insect egg

Page 21: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

SYMBIOSIS QUIZSYMBIOSIS QUIZ

Which of the following occur as a result of symbiosis?

A. Parasitism

B. Mutualism

C. Commensalism

D. All of the above

http://nsdl.org

Page 22: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

Wolbachia are Infectious Widowmakers!

ParthenogenesisParthenogenesis

FeminizationFeminization

http://nsdl.org

Male-KillingMale-Killing

Page 23: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

Wolbachia cause Infectious (River) Blindness

Horeauf et al, 2003

11 months post-treatment

Untreated

Onchocerca volvulus

http://nsdl.org

Page 24: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

What is the closest relative to Wolbachia?What is the closest relative to Wolbachia?

E. coli

Mitochondria

Preying Mantis

Giardia

http://nsdl.org

Stamp your answer

Page 25: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

How do YOU discover the Wolbachia within?

http://nsdl.org

Page 26: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

jbpc.mbl.edu/~sbordenstein/workshop.htmljbpc.mbl.edu/~sbordenstein/workshop.html

Q u ic k T ime ™ a n d aT IF F (U n c o mp re s s e d ) d e c o mp re s s o ra re n e e d e d to s e e th is p ic tu re .

Page 27: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

MBLMBL April 11-13, 2008April 11-13, 2008

Your School!Your School!

Page 28: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

Fannie Lou Hamer Freedom HS

Bronx, NY

Grade 9 & 10

When it all comes together, it looks like this!

Page 29: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

“Discover Life”

pick4.pick.uga.edu/mp/20q?guide=Insect_orders

Page 30: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

http://nsdl.org

Page 31: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

PCR is a biotechnology PCR is a biotechnology method that:method that: PCR is a biotechnology PCR is a biotechnology method that:method that:

a) Extracts DNA

b) Amplifies DNA

c) Sequences DNA

d) Expresses RNA from DNA

http://nsdl.org

Poll Question!Poll Question!

Page 32: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

Binghamton High School, NY, Dec. 2007

Page 33: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

MBLHigh School

ATGCGC

http://nsdl.org

Page 34: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

Lab 5: DNA Sequence Analysis

Bioinformatics is like using ‘Google’ for DNA sequences

http://nsdl.org

Page 35: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

http://www.ncbi.nlm.nih.gov

http://nsdl.org

Page 36: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

Copyright © Geospiza, Inc. All rights reserved.

Copyright © Geospiza, Inc. All rights reserved.

Lab 5 - BLAST tutorial: www.geospiza.com/outreach/BLAST/index.html

Copyright © Geospiza, Inc. All rights reserved.

Page 37: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

jbpc.mbl.edu/~sbordenstein/workshop.htmljbpc.mbl.edu/~sbordenstein/workshop.htmljbpc.mbl.edu/~sbordenstein/workshop.htmljbpc.mbl.edu/~sbordenstein/workshop.html

Scientist(MBL)

Educator(YOU!)

Partnership

Thanks to:

MBL Workshop, April 11-13

http://nsdl.org

Page 38: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

THANK YOU!

Dr. Seth [email protected]

http://www.mbl.edu/

http://jbpc.mbl.edu/~sbordenstein/workshop.htmlhttp://jbpc.mbl.edu/~sbordenstein/workshop.html

Sarah [email protected]

Page 39: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

• Download our Seminar Resource List

• Utilize our blog featuring our presenters for the Fall Series sharing their insights on careers in science and science education:

http://expertvoices.nsdl.org/2007fall-nsta-sems/

Go to http://nsdl.org and click on the K-12 audience page to:

http://nsdl.org

Page 40: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

http://www.elluminate.com

Elluminate logo

Page 41: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

http://learningcenter.nsta.org

NLC screenshot

Page 42: LIVE INTERACTIVE LEARNING @ YOUR DESKTOP Tuesday, January 8, 2008 NSDL/NSTA Web Seminar: Discover Microbial Worlds

National Science Teachers AssociationGerry Wheeler, Executive Director

Frank Owens, Associate Executive Director Conferences and Programs

Al Byers, Assistant Executive Director e-Learning

LIVE INTERACTIVE LEARNING @ YOUR DESKTOP

NSTA Web SeminarsFlavio Mendez, Director

Danielle Troiano, Project CoordinatorJeff Layman, Technical Coordinator