lipid-dependent surface transport of the proton pumping
DESCRIPTION
Lipid-dependent surface transport of the proton pumping. ATPase: A model to study plasma membrane biogenesis in yeast. Fan Mengdi Li Yandi. The Points. Introduction Biogenesis and transport of the proton pumping H + -ATPase. The general introduction of the H+-ATPase in yeast. - PowerPoint PPT PresentationTRANSCRIPT
Lipid-dependent surface transport of the proton pumping
ATPase: A model to study plasma membrane biogenesis in yeast
Fan Mengdi Li Yandi
The Points
Introduction Biogenesis and transport of the
proton pumping H+-ATPase
The general introduction of the H+-ATPase in yeast
A good model to study plasma membrane biogenesis
The function of ATPase :
maintenance of the electrochemical gradient across the membrane
regulating intracellular PH
symporters
The general introduction
How did the H+-ATPase transport to the plasma membrane?
Begin: oligomerization and package
Surface transport of the ATPase
Raft association
Stable integrate at the plasma membrane
protein
lipid
Every step needs the help of these lipids:
How did the H+-ATPase transport to the plasma membrane?
sterolssphingolipids
Step 1: oligomerization
protein lipidProtein-lipid complex
In the ER, Pma1form a homo-oligomeric complex—the protein-lipid complex
The ER
C26Pma1
The ER
coat protein complex II(a kind of transport vesicle)
Step 2: package
Protein-lipid complex
The protein-lipid complex then packaged into a larger subclass of COPII
Step 3: modify of the Pma1
coat protein complex II
Protein-lipid complex
The Pma1 becomes phosphorylated on multiple serine and threonine residues
P-
Step 4: Pma1 transport to the cell surface
coat protein complex II
Protein-lipid complex
C26
Pma1 is transported to the cell surface
Every step needs the help of these lipids:
How did the H+-ATPase transport to the plasma membrane?
sterolssphingolipids
Step 5: Stable integrate at the plasma membrane
H+
ATP
Lipid raft association
C26
Two mutant of Pma1
Affect the stability, targeting, and detergent solubility during its transport to the surface
The first mutant:Pmal-7
coat protein complex II
Protein-lipid complex
Pmal-7:mistargeted from the Golgi to the vacuole without reaching the cell surface.
Vacuole
The second mutant:Pmal-10
C26
vacuole
Pmal-10: fails to become stabilized on the membrane, but to delivered to the vacuole, then degradation.
Two mutant of Pma1
. The mutant indicate: lipid raft association may play important roles in maintaining protein stability at the plasma membrane.