lecture 7 corynebacterium and listeria
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الرحيم الرحمن الله الرحيم بسم الرحمن الله بسم
GENUS: GENUS: CORYNEBACTERIUMCORYNEBACTERIUMProf. Khalifa Sifaw Ghenghesh
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• Diptheroides = Coryneforms
• Gram +ve,
• Nonmotile Rods.
• Arranged in V forms or Palisades
• Catalase +ve
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1. 1. Corynebacterium diphtheriaeCorynebacterium diphtheriae
• Diphtheria.
• Pleomorphic Bacilli• Cells Contain Metachromatic Granules.
Morphology
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Corynebacterium diphtheriaeCorynebacterium diphtheriaeClub-shaped, Gram-positive rodsClub-shaped, Gram-positive rods
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Cultural CharacteristicsCultural Characteristics
• i. Loeffler's Serum Slope.
• ii. Tellurite BA.
- Temp. 37oC,
- Aerobic and Facultative AnO2.
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Corynebacterium diphtheriaeCorynebacterium diphtheriae forms black forms black colonies on tellurit agar (left), on blood agar colonies on tellurit agar (left), on blood agar
colonies appear white (right)colonies appear white (right)
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PATHOGENICITYPATHOGENICITY
• Diphtheria is a Toxaemia.– Exotoxin: M. wt 62000– 2 Fragaments >> A & B– Inhibits Protein Synthesis.– Has Special Affinity for Certain Tissues
"Mycocardium, Adrenals, Nerve Endings".
• Toxin >> Toxoid.
• Site of Infection >> Faucial.– Toxin >> Pseudomemebrane.
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LABORATORY DIAGNOSISLABORATORY DIAGNOSIS
• Important >> Specific Treatment Should Be Started Immediately on Suspicion of Diphtheria.
• Specimen: Two Swabs from the Lesion.
• Culture:i. Loeffler's Serum Slope >> Albert Stain.
ii. Tellurite BA.
iii. BA >> Streptococcal or Staphylococcal Pharyngitis.
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PATHOGENICITY TESTSPATHOGENICITY TESTS
• In-vivo Tests:i. Subcutaneous Test:ii. Intracutaneuos Test:
• In-vitro Test:– Elek Test ( Gel Precipitaion Test).
• Tissue Culture:
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TREATMENTTREATMENT
• i. Antitoxin: > Moderate Cases:> Severe Cases:
• ii. Antibiotic:
• i. Active Immunization >> PDT.• ii. Passive Immunization:• iii. Combined:
PREVENTIONPREVENTION
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SCHICK TESTSCHICK TEST
• > Injection of 0.2 ml of Diphtheria Toxin Intradermally.
• > Positive Reaction >> Erythema + Swelling
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______________________________________________________________________Result Test Arm Control Arm Interpretation Diphthri
(Toxin) Inactivated Toxin Immunization
36h 120h 36h 120h______________________________________________________________________Negative -- -- -- -- Immune, Not
Not Hypersensitive Required
----------------------------------------------------------------------------------------
Positive + + -- -- Not Immune, Not RequiredHypersensitive
---------------------------------------------------------------------------------------------------------------------Negative + -- + -- Immune Not
Hypersensitive Required---------------------------------------------------------------------------------------------------------------------Positive & + + + -- Not Immune Contra-Pseudo Hypersensitive indicated______________________________________________________________________
SCHICK TESTSCHICK TEST
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الرحيم الرحمن الله الرحيم بسم الرحمن الله بسم
Listeria monocytogenesListeria monocytogenes
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• Listerosis.Listerosis.
• G+ve Coccobacilli > in Short Chains.
• Motile at 25oC But Not at 37oC.
• Aerobic to Microaerophilic.• On BA > Narrow Zone of -Haemolysis.
• Listerolysin O >
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DISEASEDISEASE
• Incidence and Transmission >
• i. In Adults:
• ii. Neonatal Infections:a. Early Type:
b. Late Type:
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LABORATORY DIAGNOSISLABORATORY DIAGNOSIS
• i. Specimen: Depend on Syndrome: Blood, CSF, Genital Tract
Secretions.
• ii. Media: Selective Media.
• Penicillin, Ampicillin.
TREATMENTTREATMENT