Lecture 11Based on Chapter 17 -Regulation of Gene Expression in Bacteria and Bacteriophages

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1 - Introduction

2 – The lac Operon of E. coli

3 – Lactose as a carbon Source for E.coli

4 - Experimental Evidence for the Regulation of lac Genes

The experiments of Jacob and Monod produced an understanding of arrangement and control of the lac genes.

5 – Mutations in the Protein-Coding Genes

6 – Mutations affecting the Regulation of Gene Expression

7 – Operator Mutations

8 – lacI Gene Regulatory Mutations

9 – Jacob & Monod’s Operon Model for the Regulation of lac Genes

Figure 17.5

Figure 17.6

10 – Induction of lac by Allolactose (lactose)

11a – Effect of lac Oc Mutations (merodiploid)

11b – Effect of lac Oc Mutations (merodiploid)

12a – Effect of lacI- Mutations

12b – Effect of lacI- Mutations

12c – Effect of lacI- Mutations

13 – Additional lacI Mutations

14 – Positive control of the lac Operon

15 – Molecular Details of lac Operon Regulation

16 - The trp Operon of E. coli

17 – Molecular Model for Attenuation

18 – Molecular Model for Attenuation

19 – Genetic Evidence for Attenuation

20 – Attenuation at Other Operons

21 – The ara Operon of E. coli: Positive and Negative Control

22 – Regulation of Gene Expression in Phage Lambda

23 – Early Transcription Events

24 – The Lysogenic Pathway

25 – The Lytic Pathway