lcms-325 tq operation chem 115 paul hrvatin winter 2013
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DESCRIPTION
LCMS-325 TQ Operation Figure 5Figure 6 Figure 7Figure 8 3 cont. The spray icon now has a red stream coming from it (Figure 6) 4.A few minutes is required for the temperature to stabilize, load your sample into the sample loop during this time making sure the valve is in the load position. 5.Under the file tab In System Control, open up the sample list and set the data file and destination. Toggle back to the Quad View using the windows tab. 6.Once the API and 325 indicate they are ready (Figure 7), its time turn on the analyzer. The button turns green when it has been activated (Figure 8). 7.Toggle back to the sample list using the windows tab under System Control and click Begin on the sample list. This starts the automation. 8.Toggle back to the LC pump window and click Start Run on the pumps. This starts the run and the green analysis timer begins in the area to the right of ‘325 MS: ready to collect’ seen in Figure 5. Push the valve button to inject your first sample (water blank) into the LCMS. 9.Fill the syringe barrel with water and flush out the injection port 2-3 times with a full 25µL each flush. Pull up 10-15µL of the next sample into the syringe, switch the valve to load, and fill the loop with the next sample. Repeat this step as necessary and inject your ubiquitin samples in 2 minute intervals.