lcd-array kit meat 5.0 - processed samples · all seven samples (a-g) and one zno template control...

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Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485 1 | Page CHIPRON GmbH │ Eresburgstr. 22-23 D-12103 BERLIN │ T: +49 30 787994 70 F: +49 30 78794 99 [email protected] LCD-Array Kit MEAT 5.0 - Processed Samples Analysis of DNA extracts from canned, minced meat mixtures treated with heat to different extends. All samples contained 50% meat from different species and 50% additional ingredients composed of oil, water (ice), spices and salts 1) . Meat of seven animal species has been mixed at different ratios. The sample material was minced, canned and heat treated in 2002 and stored for 1 year without cooling followed by 12 years at -20°C prior to DNA extraction 2) . Sample composition: Meat % Product % Meat Species 1 45,70 91,40 Species 2 2,50 5,00 Species 3 1,00 2,00 Species 4 0,50 1,00 Species 5 0,25 0,50 Species 6 0,05 0,10 Species 7 0,00 0,00 O,I,Sa,Sp 50,00 - Total 100,00 100,00 Relative content Samples % Product % Meat A B C D E F G 45,70 91,40 Pork Chicken Turkey Sheep Duck Cattle Horse 2,50 5,00 Chicken Turkey Sheep Duck Cattle Horse Pork 1,00 2,00 Turkey Sheep Duck Cattle Horse Pork Chicken 0,50 1,00 Sheep Duck Cattle Horse Pork Chicken Turkey 0,25 0,50 Duck Cattle Horse Pork Chicken Turkey Sheep 0,05 0,10 Cattle Horse Pork Chicken Turkey Sheep Duck 0,00 0,00 Horse Pork Chicken Turkey Sheep Duck Cattle 50,00 - O,I,Sa,Sp O,I,Sa,Sp O,I,Sa,Sp O,I,Sa,Sp O,I,Sa,Sp O,I,Sa,Sp O,I,Sa,Sp 100,00 100,00 1) Additional ingredients: vegetable oil (25%), water (23%), NaCl (1.5%), phosphate (0.22%), ascorbate (0.03%), spices (0.25%). -> Spices: Pepper (62 %), nutmeg (25,2 %), cardamom (12 %), cinnamom (0,8 %) 2) Samples have been prepared within the funded Fifth Framework Programme of the European Commission (Project ID: MOLSPEC-ID, QLK1-2001-02373) and have kindly been provided by TIB Molbiol S.r.l, Italy.

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Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

1 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

LCD-Array Kit MEAT 5.0 - Processed Samples

Analysis of DNA extracts from canned, minced meat mixtures treated with heat to different

extends. All samples contained 50% meat from different species and 50% additional ingredients

composed of oil, water (ice), spices and salts 1). Meat of seven animal species has been mixed

at different ratios. The sample material was minced, canned and heat treated in 2002 and

stored for 1 year without cooling followed by 12 years at -20°C prior to DNA extraction 2).

Sample composition:

Meat % Product % Meat

Species 1 45,70 91,40

Species 2 2,50 5,00

Species 3 1,00 2,00

Species 4 0,50 1,00

Species 5 0,25 0,50

Species 6 0,05 0,10

Species 7 0,00 0,00

O,I,Sa,Sp 50,00 -

Total 100,00 100,00

Relative content Samples

% Product % Meat

A B C D E F G

45,70 91,40 Pork Chicken Turkey Sheep Duck Cattle Horse

2,50 5,00 Chicken Turkey Sheep Duck Cattle Horse Pork

1,00 2,00 Turkey Sheep Duck Cattle Horse Pork Chicken

0,50 1,00 Sheep Duck Cattle Horse Pork Chicken Turkey

0,25 0,50 Duck Cattle Horse Pork Chicken Turkey Sheep

0,05 0,10 Cattle Horse Pork Chicken Turkey Sheep Duck

0,00 0,00 Horse Pork Chicken Turkey Sheep Duck Cattle

50,00 - O,I,Sa,Sp O,I,Sa,Sp O,I,Sa,Sp O,I,Sa,Sp O,I,Sa,Sp O,I,Sa,Sp O,I,Sa,Sp

100,00 100,00

1) Additional ingredients: vegetable oil (25%), water (23%), NaCl (1.5%), phosphate (0.22%), ascorbate (0.03%), spices

(0.25%). -> Spices: Pepper (62 %), nutmeg (25,2 %), cardamom (12 %), cinnamom (0,8 %)

2) Samples have been prepared within the funded Fifth Framework Programme of the European Commission (Project ID:

MOLSPEC-ID, QLK1-2001-02373) and have kindly been provided by TIB Molbiol S.r.l, Italy.

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

2 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Heat Treatment:

To measure the assay performance with sample material exposed to intensive heat treatment,

two subsets of samples A-G have been analysed in eight repetitions each.

Set 1: Cans for use under tropical conditions; calculated Fc-Value ≥ 12.0

Set 2: Cans exposed to ultra high heat treatment; calculated Fc-Value ≥ 30.0

DNA Extraction & PCR:

Total DNA was extracted using the NucleoSpin Kit FOOD [Macherey & Nagel, Germany]

according to kit instructions with the following modification: 1 g of sample material was

lysed in 2.5 ml Buffer CF and 50 µl Proteinase K. After lysis and vortexing only 600 µl of this

solution were used for the next steps. All samples were treated with RNase.

Nucleic acid concentration was measured with Nandrop 2000c® [Thermo Scientific, USA]

and all samples were adjusted to 10 ng/µl.

PCR and hybridisation was performed according to the instructions of the LCD-Array Kit

MEAT 5.0 using 50ng DNA/PCR, the HotStar Taq Plus Mastermix® [Qiagen, Germany] and a

TProfessional® Thermal Cycler [Analytic Jena AG, Germany].

Scanning and Data analysis was done with the LCD-Array CHIP-Scanner PF7250u and

SlideReader Software Vers. 12 [Chipron GmbH, Germany].

Setup & Analysis:

All seven samples (A-G) and one ‘No Template Control’ (NTC) of each run have been hybridised

to one LCD-Array chip. Data are shown only for species for which signals above the default cut

off (2000 pixel values) were detected. In none of the 64 hybridizations any species other than

those having added during preparation of sample material was detected.

Remarkably, in both samples of Set 1 (Fc-Value ≥ 12.0) with the highest concentration of Duck

meat (45.7% and 2.5%) Anas platyrhynchos was detected in addition to Cairina moschata,

indicating that the Duck meat used for sample preparation (entire batch size 10 kg/sample)

contained small amounts of material from A. platyrhynchos or cross breeds of both species.

Refer to the Evaluation Report: Specificity to see that no cross reactions can be detected with

pure DNA of both species. Therefore the signals for Mallard Duck (A. platyrhynchos) have also

been included in the data tables shown below.

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

3 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample A:

Set 1: Fc-Value ≥ 12.0 Set 2: Fc-Value ≥ 30.0

Hybridisation 1 of 8 Hybridisation 1 of 8

Data summary of all eight hybridisations:

see entire data set at the and of this report

relative content Fc-Value ≥ 12.0 Fc-Value ≥ 30.0

Meat/Species % Product % Meat Positives Mean STDEV Positives Mean STDEV

Hyb-Control - - 8 / 8 57886 772 8 / 8 53009 4412

Pork 45,70 91,40 8 / 8 55689 1171 8 / 8 51619 5617

Chicken 2,50 5,00 8 / 8 51665 2056 8 / 8 45134 4602

Turkey 1,00 2,00 8 / 8 52105 1985 8 / 8 45969 6161

Sheep 0,50 1,00 8 / 8 57993 1063 8 / 8 45448 7755

Musc. Duck 0,25 0,50 8 / 8 56035 1136 8 / 8 48112 7907

Cattle 0,05 0,10 8 / 8 26155 3907 8 / 8 7105 2685

Horse 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

4 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample B:

Set 1: Fc-Value ≥ 12.0 Set 2: Fc-Value ≥ 30.0

Hybridisation 1 of 8 Hybridisation 1 of 8

Data summary of all eight hybridisations:

see entire data set at the and of this report

relative content Fc-Value ≥ 12.0 Fc-Value ≥ 30.0

Meat/Species % Product % Meat Positives Mean STDEV Positives Mean STDEV

Hyb-Control - - 8 / 8 58039 491 8 / 8 54141 4010

Chicken 45,70 91,40 8 / 8 52393 3379 8 / 8 44236 6068

Turkey 2,50 5,00 8 / 8 53844 2525 8 / 8 48266 3912

Sheep 1,00 2,00 8 / 8 55350 1602 8 / 8 52490 3727

Musc. Duck 0,50 1,00 8 / 8 56657 1701 8 / 8 52003 6319

Cattle 0,25 0,50 8 / 8 59197 853 8 / 8 39576 10199

Horse 0,05 0,10 8 / 8 55813 1555 8 / 8 21509 8504

Pork 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

5 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample C:

Set 1: Fc-Value ≥ 12.0 Set 2: Fc-Value ≥ 30.0

Hybridisation 1 of 8 Hybridisation 1 of 8

Data summary of all eight hybridisations:

see entire data set at the and of this report

relative content Fc-Value ≥ 12.0 Fc-Value ≥ 30.0

Meat/Species % Product % Meat Positives Mean STDEV Positives Mean STDEV

Hyb-Control - - 8 / 8 58337 794 8 / 8 54291 3960

Turkey 45,70 91,40 8 / 8 56253 1445 8 / 8 47714 6069

Sheep 2,50 5,00 8 / 8 56535 2451 8 / 8 53074 3542

Musc. Duck 1,00 2,00 8 / 8 56297 1576 8 / 8 51552 5176

Cattle 0,50 1,00 8 / 8 59059 1800 8 / 8 53171 4890

Horse 0,25 0,50 8 / 8 57808 1422 8 / 8 45960 8689

Pork 0,05 0,10 8 / 8 30005 3948 5 / 8 4025 3847

Chicken 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

6 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample D:

Set 1: Fc-Value ≥ 12.0 Set 2: Fc-Value ≥ 30.0

Hybridisation 1 of 8 Hybridisation 1 of 8

Data summary of all eight hybridisations:

see entire data set at the and of this report

relative content Fc-Value ≥ 12.0 Fc-Value ≥ 30.0

Meat/Species % Product % Meat Positives Mean STDEV Positives Mean STDEV

Hyb-Control - - 8 / 8 57892 1076 8 / 8 54558 3656

Sheep 45,70 91,40 8 / 8 54403 2899 8 / 8 51898 2959

Musc. Duck 2,50 5,00 8 / 8 47613 4788 8 / 8 51959 4103

Cattle 1,00 2,00 8 / 8 57908 1093 8 / 8 45723 8382

Horse 0,50 1,00 8 / 8 56266 1043 8 / 8 38042 9987

Pork 0,25 0,50 8 / 8 52694 1252 8 / 8 19038 9006

Chicken 0,05 0,10 8 / 8 36135 3341 8 / 8 4319 1659

Turkey 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Mallard Duck 0,00 0,00 8 / 8 12947 2505 0 / 0 0 0

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

7 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample E:

Set 1: Fc-Value ≥ 12.0 Set 2: Fc-Value ≥ 30.0

Hybridisation 1 of 8 Hybridisation 1 of 8

Data summary of all eight hybridisations:

see entire data set at the and of this report

relative content Fc-Value ≥ 12.0 Fc-Value ≥ 30.0

Meat/Species % Product % Meat Positives Mean STDEV Positives Mean STDEV

Hyb-Control - - 8 / 8 58292 615 8 / 8 53496 3816

Musc. Duck 45,70 91,40 8 / 8 54301 2686 8 / 8 51518 4589

Cattle 2,50 5,00 8 / 8 59534 952 8 / 8 57659 1743

Horse 1,00 2,00 8 / 8 58558 1251 8 / 8 54292 4292

Pork 0,50 1,00 8 / 8 57047 1198 8 / 8 32285 13797

Chicken 0,25 0,50 8 / 8 38033 2519 8 / 8 6422 2027

Turkey 0,05 0,10 8 / 8 15325 1510 1 / 8 278 737

Sheep 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Mallard Duck 0,00 0,00 8 / 8 6738 1283 0 / 0 0 0

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

8 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample F:

Set 1: Fc-Value ≥ 12.0 Set 2: Fc-Value ≥ 30.0

Hybridisation 1 of 8 Hybridisation 1 of 8

Data summary of all eight hybridisations:

see entire data set at the and of this report

relative content Fc-Value ≥ 12.0 Fc-Value ≥ 30.0

Meat/Species % Product % Meat Positives Mean STDEV Positives Mean STDEV

Hyb-Control - - 8 / 8 57583 1047 8 / 8 52482 4141

Cattle 45,70 91,40 8 / 8 58205 883 8 / 8 54302 3950

Horse 2,50 5,00 8 / 8 58359 724 8 / 8 52676 4351

Pork 1,00 2,00 8 / 8 54082 2232 8 / 8 31033 9076

Chicken 0,50 1,00 8 / 8 55306 1225 8 / 8 31394 9519

Turkey 0,25 0,50 8 / 8 57568 1578 8 / 8 43409 7381

Sheep 0,05 0,10 8 / 8 8557 3315 0 / 0 0 0

Musc. Duck 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

9 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample G:

Set 1: Fc-Value ≥ 12.0 Set 2: Fc-Value ≥ 30.0

Hybridisation 1 of 8 Hybridisation 1 of 8

Data summary of all eight hybridisations:

see entire data set at the and of this report

relative content Fc-Value ≥ 12.0 Fc-Value ≥ 30.0

Meat/Species % Product % Meat Positives Mean STDEV Positives Mean STDEV

Hyb-Control - - 8 / 8 58088 1206 8 / 8 53062 3497

Horse 45,70 91,40 8 / 8 57094 1012 8 / 8 50778 3613

Pork 2,50 5,00 8 / 8 58041 1023 8 / 8 50334 6234

Chicken 1,00 2,00 8 / 8 56372 1404 8 / 8 46441 6476

Turkey 0,50 1,00 8 / 8 55376 2251 8 / 8 45822 6333

Sheep 0,25 0,50 8 / 8 44044 3062 8 / 8 13780 7257

Musc. Duck 0,05 0,10 8 / 8 45730 6626 8 / 8 8088 3141

Cattle 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 / 0 0 0

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

10 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

NTC:

Set 1: Fc-Value ≥ 12.0 Set 2: Fc-Value ≥ 30.0

Hybridisation 1 of 8 Hybridisation 1 of 8

Data summary of all eight hybridisations:

see entire data set at the and of this report

relative content Fc-Value ≥ 12.0 Fc-Value ≥ 30.0

Meat/Species % Product % Meat Positives Mean STDEV Positives Mean STDEV

Hyb-Control - - 8 / 8 58044 994 8 / 8 53347 3556

Horse - - 0 / 8 0 0 0 / 8 0 0

Pork - - 0 / 8 0 0 0 / 8 0 0

Chicken - - 0 / 8 0 0 0 / 8 0 0

Turkey - - 0 / 8 0 0 0 / 8 0 0

Sheep - - 0 / 8 0 0 0 / 8 0 0

Musc. Duck - - 0 / 8 0 0 0 / 8 0 0

Cattle - - 0 / 8 0 0 0 / 8 0 0

Mallard Duck - - 0 / 0 0 0 0 / 0 0 0

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

11 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Data Set Samples A-G │ Fc-Value ≥ 12.0 │ 8 repetitions each

Sample A relative content

Meat/Species % Product % Meat A1 A2 A3 A4 A5 A6 A7 A8 Mean STDEV

Pork 45,70 91,40 8 / 8 54720 53817 56384 56648 55208 55852 55076 57808 55689 1171

Chicken 2,50 5,00 8 / 8 49722 52785 49682 53534 49262 50005 53774 54559 51665 2056

Turkey 1,00 2,00 8 / 8 49771 51332 54734 53012 54958 49356 50864 52812 52105 1985

Sheep 0,50 1,00 8 / 8 56904 56754 58392 59062 57534 57668 57523 60108 57993 1063

Musc. Duck 0,25 0,50 8 / 8 56653 55830 56322 54156 54892 57967 56994 55468 56035 1136

Cattle 0,05 0,10 8 / 8 29615 25360 20114 29525 20064 29444 25341 29776 26155 3907

Horse 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 56466 57351 59043 57854 58365 57295 58125 58590 57886 772

Sample B relative content

Meat/Species % Product % Meat B1 B2 B3 B4 B5 B6 B7 B8 Mean STDEV

Chicken 45,70 91,40 8 / 8 46540 53313 53887 54761 53416 46918 54120 56190 52393 3379

Turkey 2,50 5,00 8 / 8 52026 50758 58088 53663 57398 52610 51543 54668 53844 2525

Sheep 1,00 2,00 8 / 8 54804 53444 58229 54174 57704 55238 54248 54962 55350 1602

Musc. Duck 0,50 1,00 8 / 8 57557 55084 59006 54205 58506 57996 55768 55130 56657 1701

Cattle 0,25 0,50 8 / 8 59673 58326 59074 58800 58126 60890 58793 59894 59197 853

Horse 0,05 0,10 8 / 8 53537 54624 57347 56968 56585 54084 55270 58092 55813 1555

Pork 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 57287 57845 58319 57891 57523 58035 58525 58890 58039 491

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

12 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample C relative content

Meat/Species % Product % Meat C1 C2 C3 C4 C5 C6 C7 C8 Mean STDEV

Turkey 45,70 91,40 8 / 8 54598 54464 58033 56946 57246 55238 55230 58269 56253 1445

Sheep 2,50 5,00 8 / 8 57908 52184 59075 55712 58104 59022 53192 57086 56535 2451

Musc. Duck 1,00 2,00 8 / 8 55644 53484 58756 56111 57509 56476 54795 57602 56297 1576

Cattle 0,50 1,00 8 / 8 57420 56502 61903 59242 60968 58432 57498 60509 59059 1800

Horse 0,25 0,50 8 / 8 56254 55699 59304 58851 58044 57124 57140 60046 57808 1422

Pork 0,05 0,10 8 / 8 27352 25992 29796 35382 28312 28032 27463 37712 30005 3948

Chicken 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 57513 57207 59843 57744 58743 58409 58350 58884 58337 794

Sample D relative content

Meat/Species % Product % Meat D1 D2 D3 D4 D5 D6 D7 D8 Mean STDEV

Sheep 45,70 91,40 8 / 8 55106 50497 58803 51846 58108 56028 51601 53238 54403 2899

Musc. Duck 2,50 5,00 8 / 8 50108 38852 49546 50814 48614 50638 40162 52166 47613 4788

Cattle 1,00 2,00 8 / 8 56562 56650 59772 57340 59234 57954 57324 58425 57908 1093

Horse 0,50 1,00 8 / 8 56272 54356 56747 56550 55868 57147 55254 57934 56266 1043

Pork 0,25 0,50 8 / 8 51214 51964 54896 50860 53426 52582 53702 52910 52694 1252

Chicken 0,05 0,10 8 / 8 35806 30496 40950 35162 39736 36540 32162 38224 36135 3341

Turkey 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 8 / 8 9276 12522 13892 15149 13584 9070 13230 16854 12947 2505

Hyb-Control - - 8 / 8 57005 56134 59429 58180 58682 57806 56878 59024 57892 1076

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

13 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample E relative content

Meat/Species % Product % Meat E1 E2 E3 E4 E5 E6 E7 E8 Mean STDEV

Musc. Duck 45,70 91,40 8 / 8 54556 49370 57492 54902 56210 55279 50454 56144 54301 2686

Cattle 2,50 5,00 8 / 8 57981 58447 60860 60220 59694 59203 59234 60634 59534 952

Horse 1,00 2,00 8 / 8 59075 56523 59928 58530 59385 59796 56555 58668 58558 1251

Pork 0,50 1,00 8 / 8 58167 55004 57318 56339 56152 59034 56586 57772 57047 1198

Chicken 0,25 0,50 8 / 8 34313 36194 40162 39484 39012 35094 37820 42186 38033 2519

Turkey 0,05 0,10 8 / 8 16300 17068 12454 15988 13280 15636 16460 15414 15325 1510

Sheep 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 8 / 8 7418 8510 5243 5810 5249 6897 8688 6088 6738 1283

Hyb-Control - - 8 / 8 57585 57471 59431 57756 58527 58488 58380 58700 58292 615

Sample F relative content

Meat/Species % Product % Meat F1 F2 F3 F4 F5 F6 F7 F8 Mean STDEV

Cattle 45,70 91,40 8 / 8 57216 57081 59749 58048 59214 57834 57782 58714 58205 883

Horse 2,50 5,00 8 / 8 58241 57362 59625 57350 58829 58927 58214 58322 58359 724

Pork 1,00 2,00 8 / 8 56798 52934 51509 54228 50644 57421 53823 55297 54082 2232

Chicken 0,50 1,00 8 / 8 55314 53380 57448 53852 56258 56059 55003 55136 55306 1225

Turkey 0,25 0,50 8 / 8 57468 54953 60062 56746 59352 58149 55960 57855 57568 1578

Sheep 0,05 0,10 8 / 8 8094 13844 4687 7758 4843 7628 13916 7682 8557 3315

Musc. Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 56085 56796 59463 57278 58590 56697 57487 58266 57583 1047

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

14 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample G relative content

Meat/Species % Product % Meat G1 G2 G3 G4 G5 G6 G7 G8 Mean STDEV

Horse 45,70 91,40 8 / 8 55269 56640 58202 57262 57044 56100 57718 58516 57094 1012

Pork 2,50 5,00 8 / 8 57928 56126 59580 57234 58480 58989 57458 58535 58041 1023

Chicken 1,00 2,00 8 / 8 57444 54193 56028 56200 54956 58770 55751 57632 56372 1404

Turkey 0,50 1,00 8 / 8 54426 51656 58896 55248 57786 55476 52914 56609 55376 2251

Sheep 0,25 0,50 8 / 8 43936 44013 40210 46773 38793 44479 45244 48906 44044 3062

Musc. Duck 0,05 0,10 8 / 8 35251 42792 50755 52121 48560 36658 45246 54456 45730 6626

Cattle 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 57159 56001 60035 57870 58950 58293 57257 59140 58088 1206

Meat/Species NTC-1 NTC-2 NTC-3 NTC-4 NTC-5 NTC-6 NTC-7 NTC-8 Average STDEV.P

Cattle 0 0 0 0 0 0 0 0 0 0

Sheep 0 0 0 0 0 0 0 0 0 0

Equine 0 0 0 0 0 0 0 0 0 0

Pork 0 0 0 0 0 0 0 0 0 0

Chicken 0 0 0 0 0 0 0 0 0 0

Turkey 0 0 0 0 0 0 0 0 0 0

Muscovy Duck 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0 0 0 0 0 0 0 0 0 0

Hyb-Ctrl 58225 56209 57410 58571 57043 58567 59288 59041 58044 994

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

15 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Data Set Samples A-G │ Fc-Value ≥ 30.0 │ 8 repetitions each

Sample A relative content

Meat/Species % Product % Meat A1 A2 A3 A4 A5 A6 A7 A8 Mean STDEV

Pork 45,70 91,40 8 / 8 56960 57600 41094 54984 44329 52886 54730 50368 51619 5617

Chicken 2,50 5,00 8 / 8 51776 50587 37778 46092 41214 44481 48040 41100 45134 4602

Turkey 1,00 2,00 8 / 8 51499 52092 33945 47206 38073 50961 47222 46752 45969 6161

Sheep 0,50 1,00 8 / 8 53136 56387 37694 52658 41304 37620 49216 35566 45448 7755

Musc. Duck 0,25 0,50 8 / 8 56381 57654 42325 54192 45890 38786 53691 35978 48112 7907

Cattle 0,05 0,10 8 / 8 10704 8618 6908 7054 8588 3182 9200 2587 7105 2685

Horse 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 58011 58087 45333 55748 48533 52522 55939 49901 53009 4412

Sample B relative content

Meat/Species % Product % Meat B1 B2 B3 B4 B5 B6 B7 B8 Mean STDEV

Chicken 45,70 91,40 8 / 8 48089 53624 33816 49370 37277 45308 44776 41626 44236 6068

Turkey 2,50 5,00 8 / 8 55571 47652 46398 42686 49636 47889 52146 44146 48266 3912

Sheep 1,00 2,00 8 / 8 56408 57146 45496 54292 48482 53901 53436 50761 52490 3727

Musc. Duck 0,50 1,00 8 / 8 58678 60362 43002 56995 46660 49078 56014 45232 52003 6319

Cattle 0,25 0,50 8 / 8 52848 51148 30792 45294 34524 28524 47886 25593 39576 10199

Horse 0,05 0,10 8 / 8 30374 33846 10874 28388 12941 16012 26066 13574 21509 8504

Pork 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 59180 58565 48446 56459 50825 52472 57555 49623 54141 4010

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

16 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample C relative content

Meat/Species % Product % Meat C1 C2 C3 C4 C5 C6 C7 C8 Mean STDEV

Turkey 45,70 91,40 8 / 8 46170 56346 37870 53356 40980 52690 44124 50174 47714 6069

Sheep 2,50 5,00 8 / 8 58524 56966 48678 53956 51488 51145 55558 48278 53074 3542

Musc. Duck 1,00 2,00 8 / 8 58398 56866 42868 53314 45979 51328 55672 47994 51552 5176

Cattle 0,50 1,00 8 / 8 58852 59795 47943 56378 51448 49605 55640 45706 53171 4890

Horse 0,25 0,50 8 / 8 52064 58254 31429 54130 34858 47040 47318 42584 45960 8689

Pork 0,05 0,10 5 / 8 6160 10800 0 8154 0 2583 4499 0 4025 3847

Chicken 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 59371 58581 48058 56076 50542 53314 57667 50717 54291 3960

Sample D relative content

Meat/Species % Product % Meat D1 D2 D3 D4 D5 D6 D7 D8 Mean STDEV

Sheep 45,70 91,40 8 / 8 55873 54968 46890 53072 48920 51596 54104 49763 51898 2959

Musc. Duck 2,50 5,00 8 / 8 57154 55904 44816 53249 47236 52226 55408 49681 51959 4103

Cattle 1,00 2,00 8 / 8 56412 54370 37192 50876 40080 37612 53936 35304 45723 8382

Horse 0,50 1,00 8 / 8 48658 51604 28322 46522 31912 28363 43913 25038 38042 9987

Pork 0,25 0,50 8 / 8 26571 32724 10776 28047 12561 9908 23267 8446 19038 9006

Chicken 0,05 0,10 8 / 8 4922 7510 3156 5992 3896 2094 4128 2851 4319 1659

Turkey 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 59995 57137 50947 55143 52927 51459 59170 49682 54558 3656

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

17 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample E relative content

Meat/Species % Product % Meat E1 E2 E3 E4 E5 E6 E7 E8 Mean STDEV

Musc. Duck 45,70 91,40 8 / 8 54168 57388 43291 55548 45199 52932 52505 51109 51518 4589

Cattle 2,50 5,00 8 / 8 59693 59760 54642 58594 56740 57902 58368 55571 57659 1743

Horse 1,00 2,00 8 / 8 58158 60578 46742 58014 51037 54468 54422 50917 54292 4292

Pork 0,50 1,00 8 / 8 43802 51739 18354 47504 21002 18922 39686 17272 32285 13797

Chicken 0,25 0,50 8 / 8 8928 9310 3648 7734 4532 5174 7328 4720 6422 2027

Turkey 0,05 0,10 1 / 8 0 0 0 2227 0 0 0 0 278 737

Sheep 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 57481 58524 47156 56149 49628 52979 55516 50531 53496 3816

Sample F relative content

Meat/Species % Product % Meat F1 F2 F3 F4 F5 F6 F7 F8 Mean STDEV

Cattle 45,70 91,40 8 / 8 57967 58794 46844 56454 49424 55342 56464 53128 54302 3950

Horse 2,50 5,00 8 / 8 57368 58569 45510 55613 48760 52392 54510 48684 52676 4351

Pork 1,00 2,00 8 / 8 40207 44330 19374 38652 22355 25620 35571 22157 31033 9076

Chicken 0,50 1,00 8 / 8 41018 44479 19554 39498 21636 24766 37605 22595 31394 9519

Turkey 0,25 0,50 8 / 8 48214 54981 31643 50563 34976 43125 44474 39298 43409 7381

Sheep 0,05 0,10 0 / 0 0 0 0 0 0 0 0 0 0 0

Musc. Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 57977 56897 49314 54017 51467 48452 55992 45738 52482 4141

Technology for your daily routine Certified according to DIN EN ISO:9001 + DIN EN ISO:13485

18 | P a g e CHIPRON GmbH │ Eresburgstr. 22-23 │ D-12103 BERLIN │ T: +49 30 787994 70 │ F: +49 30 78794 99 │ [email protected]

Sample G relative content

Meat/Species % Product % Meat G1 G2 G3 G4 G5 G6 G7 G8 Mean STDEV

Horse 45,70 91,40 8 / 8 53334 56326 44358 53554 47024 52050 50308 49273 50778 3613

Pork 2,50 5,00 8 / 8 55357 59939 43096 56978 46386 46376 52109 42430 50334 6234

Chicken 1,00 2,00 8 / 8 51568 56593 41520 52992 43847 40064 47953 36988 46441 6476

Turkey 0,50 1,00 8 / 8 49816 56414 38476 53106 41806 41720 46628 38606 45822 6333

Sheep 0,25 0,50 8 / 8 18246 25957 7138 22086 8530 7014 15473 5792 13780 7257

Musc. Duck 0,05 0,10 8 / 8 13437 9080 7672 6880 8959 4258 11132 3289 8088 3141

Cattle 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0,00 0,00 0 / 0 0 0 0 0 0 0 0 0 0 0

Hyb-Control - - 8 / 8 56846 58220 48089 55564 50310 51908 54399 49162 53062 3497

Meat/Species NTC-1 NTC-2 NTC-3 NTC-4 NTC-5 NTC-6 NTC-7 NTC-8 Average STDEV.P

Cattle 0 0 0 0 0 0 0 0 0 0

Sheep 0 0 0 0 0 0 0 0 0 0

Equine 0 0 0 0 0 0 0 0 0 0

Pork 0 0 0 0 0 0 0 0 0 0

Chicken 0 0 0 0 0 0 0 0 0 0

Turkey 0 0 0 0 0 0 0 0 0 0

Muscovy Duck 0 0 0 0 0 0 0 0 0 0

Mallard Duck 0 0 0 0 0 0 0 0 0 0

Hyb-Ctrl 58544 56329 57279 54365 51763 50167 49922 48409 53347 3556