laura innes graduate students: eric kalman, matt powell prof. zuzanna siwy
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Looking at Single Molecules with Nanopores. Laura Innes Graduate Students: Eric Kalman, Matt Powell Prof. Zuzanna Siwy Department of Physics and Astronomy University of California, Irvine. Why are Nanopores Interesting?. - PowerPoint PPT PresentationTRANSCRIPT
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Laura InnesLaura InnesGraduate Students: Eric Kalman, Matt PowellGraduate Students: Eric Kalman, Matt Powell
Prof. Zuzanna SiwyProf. Zuzanna Siwy
Department of Physics and AstronomyDepartment of Physics and AstronomyUniversity of California, IrvineUniversity of California, Irvine
Looking at Single Molecules Looking at Single Molecules with Nanoporeswith Nanopores
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Why are Nanopores Interesting?Why are Nanopores Interesting?
Biological channels are the basis of many physiological processes e.g. heart and muscle function and ‘wiring’ information from the brain to all the cells.
Nanopores are the basis for biosensors detecting single molecules
http://www1.engr.ucsb.edu/~saleh/Research/Saleh_Omar_Thesis.pdf
http://en.wikipedia.org/wiki/Cell_membrane
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Making NanoporesMaking Nanopores
Creation of single nanopores starts at the GSI accelerator center in Darmstadt, Germany
Irradiated with a single ion of Au or U, at 2 GeV (15% c)
Deposition of energy from the swift, heavy ion creates a damage track in polymer
Chemical etching – ‘developing’ of the track created by single heavy ions
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EquipmentEquipment
Conductivity Cell Faraday Cage
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Etching CurrentEtching Current
1000 mV
pA
pA
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Gold Replica of a Single NanoporeGold Replica of a Single Nanopore
Scopece et al. Nanotechnology 17 (2006) 3951.
Formulas
Dd
LR
4
minmin/ 13.22 etchingtnmD
R – ionic resistance of a nanopore - specific conductivity of electrolyteL – membrane thickness
D
d
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Why a Conical Pore?Why a Conical Pore?
L L
d d
D
Icone >> Icylinder
DdL
Rcone4
2
4
d
LRcylinder <<
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Graph of Voltage Profile inside a NanoporeGraph of Voltage Profile inside a Nanopore
x x
x
V
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Structure of DendrimersStructure of Dendrimers
~3 nm
http://www.fda.gov/NANOTECHNOLOGY/powerpoint_conversions/chbsa-nanotech-presentation06-05_files/images/image4.png
H+
H+
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Basic Physical Chemistry Basic Physical Chemistry of a Charged Particle in a Solutionof a Charged Particle in a Solution
ƒ F
ΔV - potential difference between two electrodes
l - distance apart
ƒ - frictional force
u - mobility of the ion
ze - charge
s - drift speed
l
VE
l
VzeF
uEs
af 6
Ff
a
zeu
6
When
2 a
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I
U
1.0 M KCl1.0 M KCl
Dendrimer
Detecting DendrimersDetecting Dendrimers
current
time
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Dendrimers at Various pH’sDendrimers at Various pH’s
pH 6 pH 8
pH 7 pH 10
l
VzeF
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pH 10pH 10
- 1000 mV
KCl + dendrimers
KCl without dendrimers
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pH 6pH 6
-1000 mV
-1000 mV
no dendrimers
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pH 8pH 8
-200 mV
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-800 mV
pH 8pH 8
Occasional openings
Blockage of the pore
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-1000 mV
pH 8pH 8
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pH 7pH 7
-300 mV
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-1000 mV
pH 7pH 7
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pH 7.5pH 7.5
-1000 mV
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Close up of -1000 mV
pH 7.5pH 7.5
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Lower VoltagesLower Voltages
1. Force of Friction
2. Entropy
ƒ F
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There is a Voltage Threshold for the There is a Voltage Threshold for the Dendrimers to Enter a NanoporeDendrimers to Enter a Nanopore
-100 mV
-1000 mV
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Future StudiesFuture Studies
current
time
• Continue to study the behavior of single molecules and nanopores
• Hopefully create an electromechanical valve
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-700 mV
Is It a Single Dendrimer?Is It a Single Dendrimer?
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- 900 mv
pH 6pH 6
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-900 mV
pH 8pH 8