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ROSS ElectrodesTraining

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ROSS Electrodes

• Premium pH electrode available

• Specially formulated reference system provides quickest response at temperature changes

• Offer less drift and greater stability than other pH electrodes

• Double junction reference system

• Can be used with biotech & TRIS samples

• Ability to use different fill solutions based on sample composition

• Environmentally friendly

• Also available as Triode

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ROSS Electrode Specifications

• Drift: Less than 0.002 pH units/day, minimizes restandardization

• Accuracy of measuring a pH 6.86 buffer after standardization at 25°C: Accurate within 0.03 pH for buffer at any temperature between 0-100°C using automatic temperature compensation.

• Speed of response in 6.86 buffer going from 25° C to 75°C: Response stable to 0.01 pH within 30 seconds.

• Speed of response between 6.86 and 4.01 buffers at 25°C: Response stable to 0.002 pH within 15 seconds.

• Precision: 0.01 pH

• Triode temperature accuracy: Accurate to +/- 1o C within 2 minutes

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ROSS Temperature Response

• ROSS reference system uses iodide/iodine which is water soluble. ROSS reference system is independent of temperature changes.

• Platinum in reference system prevents junction problems caused in conventional electrodes by temperature changes

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ROSS pH Electrode Families

• Standard ROSS electrodes• Wide variety to choose from

• Meets all ROSS specifications

• 12 month warranty

• Available with BNC connectors, screw caps and US standard connectors

• ROSS Sure-Flow• Unique free flowing junction prevents clogging

• 3 models to choose from

• Available mostly as BNC connector, but also EDIN

• ROSS Ultra• Improved reference system

• Less drift than standard ROSS models

• Available with BNC only

• 24 month warranty

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ROSS pH Electrode Families

• ROSS Ultra Triodes• Newest Line of ROSS• Only ROSS available as a low-maintenance electrode• 4 models as epoxy refillable• 4 models as epoxy gel• 1 model to soon be released as glass refillable• Different connectors for thermistor to fit different meters

• PerpHecT ROSS• For use with LogR meters• Utililize glass pH membrane to measure temperature• Temperature accuracy of 0.5oC• Variety of models available• Available only with BNC connector

• ROSS Micro• 1 model with BNC connector• For small sample size• Also can be used with LogR meters

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Reference junctions Used in ROSS Electrodes

• Ceramic – Used in most glass refillable electrodes

• Glass fiber – Used in most refillable epoxy electrodes

• Glass Capillary – New junction! Used in new ROSS gel triode

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Reference junctions Used in ROSS Electrodes

• Sure-Flow – Glass to glass or glass to epoxy junction

• Glass sleeve – Specialty electrode, used in 8162SC

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Electrode Connectors –

• BNC Connector• Waterproof BNC

• US Standard Connector

• Screw Cap Electrode

• Pin Tip Connector

(for reference half cell)

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ROSS Half Cell Electrodes – Standard & Ultra

800300

Sure Flow Reference Electrode

8101BNWP

pH Half Cell

800500U

ROSS Ultra Reference Electrode

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Standard ROSS Line – Glass Electrodes

8102BN

Standard size

120 X 12mm

8103BN

Semi-Micro

6mm tip

8104BN

Rugged bulb

8162SC

Fixed glass sleeve titration

joint 14/15

8163BNWP

Spear tip

For piercing, cheese,

fruits

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Standard ROSS Line – Epoxy Electrodes

8115BN

Semi-Micro

6mm tip

8135BN

Flat surface

For agar gel plates, cheese & meats

815600

Standard size for more rugged use

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ROSS Sure-Flow Electrodes

8165BNWP

Epoxy Body

Standard size

120 X 12 mm

Has replaceable bulb guard

8172BNWP

Glass Body

Standard size

Has replaceable bulb guard

8175BNWP

Epoxy body

Semi-micro

8mm tip

Has replaceable bulb guard

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PerpHecT ROSS Line

8202BN

Glass body

Standard size

120 X 12mm

8203BN

Glass Body

Semi-Micro

6mm tip

8220BNWP

Glass Body

Micro tip

3mm diameter

8235BN

Epoxy body

Flat surface

For moist surfaces

8272BN

Glass Body

Sure-Flow

Has replaceable bulb guard

8256

Epoxy body

Standard size

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ROSS Ultra Electrodes

8102BNUWP

Glass body

Standard size

120 X 12mm

8103BNUWP

Glass Body

Semi-Micro

6mm tip

8104BNUWP

Glass Body

Rugged bulb

8115BNUWP

Epoxy body

Semi-micro

8mm tip

8156BNUWP

Epoxy Body

Standard Size

8135BNUWP

Epoxy Body

Flat Surface

Standard Size

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ROSS Ultra Epoxy Body Refillable Triodes

• Four models to accommodate different meters• Faster temperature response that Ag/AgCl triodes• Only ROSS electrodes not to have a coil• 24 month warranty• Performs extremely well in clean water samples• Able to stand up to TRIS buffers with no effect on reference junction

• 8157BNUMD – For Star meters, 1 meter cable• 8157UWMMD – For Star meters, 3 meter cable• 8157BNU – For A+ meters, 1 meter cable• 8257BNU – For PerpHecT meters, 1 meter cable

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Orion Refillable ROSS Ultra Triode Strengths

• Placement and small-diameter design of the Orion ROSS Ultra Triode thermistor makes the temperature response fast and accurate.

• The ROSS reference system makes the electrode pH response very accurate, fast, and reproducible at elevated temperatures and after use in elevated temperatures.

• Junction testing shows the linear flow glass fiber reference junction performs well: has low stir potential and accurate response upon changing the ionic strength of the sample. It does not clog when soaked in Tris Buffer solution.

• The epoxy body is durable and protects the glass bulb from damage• The refillable Orion ROSS Ultra Triode is the star performer in clean water

samples (tap and DI tested) – fast, stable, accurate. • Even when cycled with a strong acid, strong base, and high ionic strength pH buffer

6.86, response is quick, stable, and accurate.

• It also performs well in Tris Buffer at 37C and in creamy salad dressing at 35C.

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ROSS Ultra Epoxy Body Low-Maintenance Triodes

• Four models to accommodate different meters• Faster temperature response that Ag/AgCl manuals• Only ROSS electrodes not to have a coil• Only low-maintenance ROSS electrodes• Superior performance to competitors in low ionic strength solutions• More accurate and stable than competitors electrodes at

temperature extremes• 18 month warranty

• 8107BNUMD – For Star meters, 1 meter cable• 8107UWMMD – For Star meters, 3 meter cable• 8107BNU – For A+ meters, 1 meter cable• 8207BNU – For PerpHecT meters, 1 meter cable

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Competitive Comparison of Gel ROSS Ultra™ Triode in Low Ionic Strength Standard

Low Ionic Strength Standard has expected pH value of 4.005 at 0oC to 30oC

The Gel ROSS Ultra Triode has superior performance in the low ionic strength solutions; it demonstrated better accuracy and response time than the other electrodes

3.70

3.75

3.80

3.85

3.90

3.95

4.00

4.05

4.10

4.15

0 200 400 600 800 1000 1200

Response Time, sec.

pH

Re

ad

ing

Aft

er

Ca

libra

tio

n

ROSS Triode

Brand A

Brand B

Brand C

Expected

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pH Response in pH 4 Buffer at Different Temperatures

Delta from expected pH in pH 4.01 buffer at ~ 10C

-0.200

-0.100

0.000

0.100

0.200

0 200 400 600

Time, sec.

Del

ta p

H

ROSS Triode 1

ROSS Triode 2

Brand B

Brand C

Delta from expected pH in pH 4.01 buffer at ~ 55C

-0.200

-0.100

0.000

0.100

0.200

0 200 400 600

Time, sec.

Del

ta p

H

ROSS Triode 1

ROSS Triode 2

Brand B

Brand C

Delta from expected pH in pH 4.01 buffer at ~ 23C after 10C and 55C

-0.200

-0.100

0.000

0.100

0.200

0 100 200 300 400 500

Time, sec.

Del

ta p

H

ROSS Triode 1

ROSS Triode 2

Brand B

Brand C

Two ROSS Gel Triodes read close to each other and close to the expected values at about 10oC, 55oC, and again at 23oC.

Brand B is good except at cold temp., when readings deviated from the expected value (about 0.05 pH).

Brand C is less accurate when reading buffers at different temperatures; it has large deviations at cold temp (about 0.2 pH) and after return to RT (>0.1pH).

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Temperature Response at ~55C

Temperature response at ~55C in pH 4

40.0

44.0

48.0

52.0

56.0

60.0

0 100 200 300 400 500 600 700

Time, sec.

Tem

pera

ture

deg

rees

C

ROSS #1

ROSS #2

Brand B

Brand C

Temp. response ar ~ 57 in pH 10 buffer

40.0

44.0

48.0

52.0

56.0

60.0

0 100 200 300 400

Time, sec.

Tem

p. d

egre

es C

ROSS #1

ROSS #2

Brand B

Brand C

ROSS Triodes are faster at elevated temperature: they reached stable temp. readings in 30 seconds.It takes about 2 min. for Brand B and more than 5 min. for Brand C electrode to reach stable temperature readings at ~ 55C.

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ROSS Ultra Glass Triode

• Just released in July 2010• 1 model for Star meters, 8302BNUMD• First Orion glass triode• Temperature response faster than most glass triodes on market• New ceramic junction

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ROSS Electrodes

Thank you to Gayle Gleichauf and Nadia Vilissova for graphs and competitive testing data of ROSS refillable and low maintenance Triode

Thank you!