laboratory work: imnv diagnostics field and laboratory ...1m nv test.pptx . food and agriculture...
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Laboratory Work: IMNV diagnostics field and laboratory protocols(PCR, histopathology)
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Asian Regional Laboratory Testing ProgramFor Aquatic Animal Diseases 2013-2014
OIE Reference Laboratorfor White spot disease
OIE Reference Laboratorfor Infectious hypodermal and haematopoietic necrosis
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Sources of samples:1. Positive control came from Indonesia, IMNV infected P.vannamei2. Negative control came from USA, SPF P.vannamei
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Sample I.D.
Clinical sign
Scale
General information of the sample received in our regular detection test
Step1: Sample preprocessing
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Before
Homogenate
After
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Nucleic Acid Automatic Extraction System
Step2: Extract nucleic acid
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Step2: Extract nucleic acid
Nucleic Acid Automatic Extraction System
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Step3: Molecular techniques
First round
Second round
RT-PCR
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Step3: Molecular techniques
qRT-PCR
Analytik Jena
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Step3: Molecular techniques
qRT-PCR
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Thank you