laboratory exercises for male reproductive system · os penis . the glans penis and includes part...

LABORATORY EXERCISES FOR MALE REPRODUCTIVE SYSTEM

Slide #101 (1096). Testis, rat. sustentacular (“Sertoli”) cells pale nuclei of these cells are visible adjacent to the limiting membrane of the seminiferous tubules Nuclei of Sustentacular cells

THE SERTOLI CELLS (SUSTENTACULAR CELLS) EXTEND THE FULL THICKNESS OF THE WALL OF THE SEMINIFEROUS TUBULE. IN OTHER WORDS, THE SERTOLI CELLS EXTEND FROM THE BASEMENT MEMBRANE TO THE LUMEN OF THE SEMINIFEROUS TUBULE. THE FUNCTION OF THE SERTOLI CELL IS SUPPORT FOR THE DEVELOPING GERM CELLS.

Leydig cells

Spermatogonia Spermatocytes Spermatids

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Horse Sperm This EM at http://peer.tamu.edu/toolkit2.asp

Slide #101 (1096). Testis, rat. Nuclei of sustentacular or Sertoli cells

Leydig cells

Spermatogonia Spermatocytes Spermatids

seminiferous tubule lumen

Myoid cell nucleus

Spermatocyte


Slide #101 (1096). Testis, rat.

Sustentacular cell nuclei or Sertoli cells Leydig

cells

Spermatocytes

seminiferous tubule

Myoid cell WHEN A SPERMATOGOIUM CELL IS SIGNALED TO UNDERGO MEIOSIS INSTEAD OF MITOSIS, THE CELL HAS REPLICATED ITS DNA TO BECOME 4N AND HAS ENTERED INTO THE FIRST PHASE OF MEIOSIS BUT NOT YET DIVIDED, SO THAT IT IS A DIPLOID 4N CELL, WHICH IS CALLED A PRIMARY (1°) SPERMATOCYTE.

Secondary spermatocytes

Meiotic figures (metaphase plates)

spermatids

Spermatogonia

Primary S. Secondary S. spermatid


Meiosis Mitosis

Human testis Epon toluidine blue stain 19680 http://viewer.serenusview.com/LinkHandler.axd?LinkId=d51e0ced-5512-4721-96c4-f9239ff75af7

Mitotic metaphase

Meiotic metaphase I


base

lumen

Mitotic metaphase

Meiotic Metaphase I

Meiotic Metaphase II

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SEMINIFEROUS TUBULES COMPOSED OF:

SERTOLI CELLS MYOID CELL nucleus

GERM CELLS

SPERMATOGONIA, SPERMATOCYTES, SPERMATIDS Human testis

Primary SPERMATOCYTES SPERMATIDS

Slide #107 (F3- 405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat.

seminiferous tubules

epididymis

Testis

ductus deferens (tail of epididymis)

rete testis and mediastinal testis

interstitial tissue between the seminiferous tubules is quite thin.

Leydig cells produce TESTOSTERONE

http://viewer.serenusview.com/Viewer.aspx?SlideId=f7b6c8c0-d209-4159-803a-3a2d3e6b02c6


The interstitial tissue between the seminiferous tubules is quite thin

Leydig cells produce TESTOSTERONE

CCT capsule’s proper TUNICA ALBUGINIA



Sertoli cells; spermatogonia; primary spermatocytes; and early and late spermatids


spermatogonia;


Golgi phase

Cap phase

Maturation phase

Elongation phase

DEMO SLIDE BOX 100 – Testis, stallion.

sustentacular (“Sertoli”) cells;

LEYDIG (INTERSTITIAL) CELLS ARE LARGE, ROUND TO OVAL CELLS WITH ACIDOPHILIC BUT OFTEN PALELY STAINING CYTOPLASM. SOMETIMES THESE CELLS CAN APPEAR FOAMY DUE TO THE FACT THAT THEY ARE STEROID PRODUCING CELLS.

Sertoli cells; spermatogonia; primary spermatocytes; late spermatids

Leydig cells

http://viewer.serenusview.com/Viewer.aspx?SlideId=6e3fdd19-5e68-415a-9369-6624527a4216

DEMO SLIDE BOX 99 –Testis, newborn kitten.

Nuclei of sustentacular cells

gonocytes

rete testis and mediastinal testis

epididymis

http://viewer.serenusview.com/Viewer.aspx?SlideId=459bba7b-4b40-419b-a4bf-c8dd56331026

DEMO SLIDE BOX 226 – (PM-172 b). Testis, epididymis, ductus deferens, pig (immature).

gonocytes

epidermidis rete testis and mediastinal testis

Dividine gonocytes

Nuclei of sustentacular (“Sertoli”) cells;

http://viewer.serenusview.com/Viewer.aspx?SlideId=74891c6c-cbdf-4c23-ae80-ffaaab695f3e

DEMO SLIDE BOX 187 (935). Testis, boar.

Cryptorchid testis

Slide #107 Normal cat testis

DEGENERATION and loss of germ cells in the seminiferous tubules; however, it illustrates the open spaces between tall Sertoli cells where germ cells would have been located as indicated in the drawing of Sertoli cells

IF THE MALE IS A BILATERAL CRYPTORCHID THEN HE WILL BE INFERTILE; IF THE MALE IS A UNILATERAL CRYPTORCHID THEN HE likely WILL HAVE REDUCED FERTILITY.

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DEMO SLIDE BOX 189 (985) -Epididymis, goat.

ductuli aberrantes vestigial mesonephric tubules

ductuli efferentes.

ductus epididymidis.

http://viewer.serenusview.com/Viewer.aspx?SlideId=ab6a0aee-ba50-4782-bc03-3b48424fbd38

DEMO SLIDE BOX 224 – (641). Epididymis, boar.

ductus epididymidis,

ductuli efferentes

http://viewer.serenusview.com/Viewer.aspx?SlideId=0694ed92-055e-4b38-83f9-195c9e03dfd1

DEMO SLIDE BOX 101 –Ductus deferens, stallion.

thick tunica muscularis

smooth muscle

epithelium and lamina propria/submucosa

http://viewer.serenusview.com/Viewer.aspx?SlideId=746133dd-9da4-445c-a853-f7294e2929da

Slide #119 (Ed 904-160c). Ampulla, donkey.

simple or stratified cuboidal epithelium

proteinaceous material In lumen of the ampulla.

CCT and smooth muscle.

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DEMO SLIDE BOX 102 – (998.) Ampulla of ductus deferens, stallion. simple or stratified cuboidal epithelium

Smooth muscle

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Slide #126 (Dog 1-160-1). Prostate, dog. urethral lumen. TRANSITIONAL

simple cuboidal or simple columnar (like the prostate) and doesn’t have stereocilia.

http://viewer.serenusview.com/Viewer.aspx?SlideId=bd6bd2ad-d2ca-4939-9a10-39e842fc4e74

DEMO SLIDE BOX 105 – Prostate gland, dog.

Urethra with transitional epithelium

fibromuscular tissue

small ampullae of the ductus deferens

http://viewer.serenusview.com/Viewer.aspx?SlideId=6e9cb3c5-35de-4c2d-ac4e-2a0504d25537

DEMO SLIDE BOX 103 – Prostate gland (pars disseminata), bull

SKELETAL MUSCLE, WHICH IS CALLED THE URETHRALIS MUSCLE OR THE EXTERNAL URETHRAL SPHINCTER

principal duct of the vesicular glands or ductus deferens;

urethra

http://viewer.serenusview.com/Viewer.aspx?SlideId=a20a2c23-6b61-4642-803c-237e088f3974

Slide #131 (946) -- Bulbourethral gland, boar.

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Slide #137 (Ed 904-173B). Vesicular gland, stallion.

vesicular glands or seminal vesicles

seminal vesicles because they are hollow, blind sacs

http://viewer.serenusview.com/Viewer.aspx?SlideId=29e62488-88fd-4640-92e6-e928de707826

DEMO SLIDE BOX 191–(991) Bulbourethral gland, goat.

skeletal muscle fibers of the bulbospongiosus muscle.

mucous character

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DEMO SLIDE BOXES 107– Penis, dog.

tunica albuginea (of the penis CCT)

pectiniform septum - separates the paired corpora cavernosum

urethra -TRANSITIONAL

PRIMARILY SMOOTH MUSCLE ( YOU MAY SEE A FEW SKELETAL MUSCL FIBERS MIXED IN WITH THE SMOOTH MUSCLE – ESPECIALLY PROXIMALLY

dorsal penile artery, vein & nerve- MUSCULAR ARTERY

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DEMO SLIDE BOXES 108 – Penis, dog.

http://viewer.serenusview.com/Viewer.aspx?SlideId=5dd34b00-c3ce-48bf-91e7-12883a8baaca

DEMO SLIDE 193 –(1086). Penis, dog.

internal lamina of the prepuce preputial cavity skin of the penis pars longa glandis - peripherally located erectile tissue urethra corpus spongiosum - erectile tissue surrounding the urethra os penis

the glans penis and includes part of the prepuce

http://viewer.serenusview.com/Viewer.aspx?SlideId=4b1dd44f-07ee-4941-b65b-a78e7fa85180

DEMO SLIDE BOX 192 (1054). –Penis, bull calf.

corpus cavernosum penis, urethra, corpus spongiosum.

internal lamina of the prepuce are fused at this location

skin of the penis and

http://viewer.serenusview.com/Viewer.aspx?SlideId=09cda2c9-732b-4386-9b5d-4b156648faa8

Slide #56 (Pm1-166C-B1). Penis, boar (immature).

nerve, collagenous connective tissue (CCT), large vein, arterioles, venules, capillaries.

corpus cavernosum penis, the urethra, and the corpus spongiosum.

nerve

Unilocular adipose Tissue (white fat),

Small muscular artery,

http://viewer.serenusview.com/Viewer.aspx?SlideId=b001592c-4e49-43ea-92f2-5b7d36b91aa1

Human Penis – transitional epithelium and surrounding spongy cavernous of penal urethra

277

corpus cavernosum penis

urethra

Corpus spongiosum

Tunica albuginea (of the penis, CCT)

Smooth muscle

nerve

Transitional epithelium of urethra

Corpus spongiosum skin

http://viewer.serenusview.com/Viewer.aspx?SlideId=73ea92e8-6b30-41ea-ad85-fd6bd91bb962

Slide Number 1Slide Number 2Slide Number 3Slide Number 4Slide Number 5Slide Number 6SEMINIFEROUS TUBULES COMPOSED OF: Slide Number 8Slide Number 9Slide Number 10Slide Number 11Slide Number 12Slide Number 13Slide Number 14Slide Number 15Slide Number 16Slide Number 17Slide Number 18Slide Number 19Slide Number 20Slide Number 21Slide Number 22Slide Number 23Slide Number 24Slide Number 25Slide Number 26Slide Number 27Slide Number 28Slide Number 29Slide Number 30Slide Number 31Slide Number 32Human Penis – transitional epithelium and �surrounding spongy cavernous of penal urethra

laboratory exercises for male reproductive system · os penis . the glans penis and includes part...

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