LAB #3: INQUIRY INTO CELL DIVISION-MITOSISLab Requirements * 2013-2014 AP Biology

Wednesday, October 30th:

WEDNESDAY, OCTOBER 30TH:

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Field of view 1 Field of view 2

Field of view 3

PRE-LAB POINTERS:1. Include the title: Inquiry into Cell Division-

Mitosis as well as the date (10.30.2013) Abstract:

1. OBJECTIVES--List the first two bulleted objectives on the 4th page of your lab manual.

2. RELEVANT TERMS—mitosis, meiosis, MPF, CDK, cytokinesis, G1 checkpoint, G2 checkpoint

Introduction: Include background information andpurpose of the lab; We will brainstorm a

hypothesisfor part 3 as a class.

MATERIALS and PROCEDURES: You will be using

• Microscopes• Slides-prepared onion root tip• Karyotypes (3)-healthy human cells, cervical cells,

leukemiacells

Write the procedures provided in your lab manual; however,

SUMMARIZE these!

RESULTS/DATA- Include diagrams of each stage of mitosis (6 of

them)- Include the data table (table 1- Mitosis Phase Counts)- For part 3, Loss of Cell Control in Cancer,

pleaseInclude the following table:

HEALTHY CELLS CASE 1: HeLa CELLS

CASE 2: PHILADELPHIA

CHROMOSOMES# OF CHROMOSOMESAPPEARANCE

For each case, answer these questions on the bottom of page 7:1. Do your observations support your hypothesis?2. If not, what type of information might you need to know in order to

understand your observations?3. If yes, what type of information can you find that would validate your

conclusions?

DISCUSSION AND CONCLUSIONS:Provide what is asked for on your scoring guide.

In addition, you will have many LAB QUESTIONS

to answer. • PAGE 6: Two questions under heading FOR

THE LAB REPORT• PAGE 8: Case 1: 2 questions

• Case 2: 5 questions