lab presentation july 2015
TRANSCRIPT
Dan Feldman
“Nanonaps” for Photothermal therapy on Balb/Cmice with 4T1 (cancer cell line) tumors
7/6/15
ONC
F127Fig 1
700 750 800 850 9000
0.05
0.1
0.15
0.2
0.25
0.3
0.35
Wavelength (nm)
Abso
rban
ce
860 wavelength, before fridge
700 750 800 850 9000
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
Wavelength (nm)
Abso
rban
ce
880 wavelength, after 24 hours in fridge
0 0.5 1 1.5 2 2.5 3 3.5 4 4.5862864866868870872874876878880882
Wavelength effected by DCM con-centration
DCM concentration (ml)
Wav
elen
gth
0 2 4 6 8 10 12 14 16857
859
861
863
865
867
Wavelength effected by F127 con-centration
F127 concentration (ml)
Wav
elen
gth
(nm
)
Pre Wash
Wash 1
Wash 2
Wash 3
0 20 40 60 80100
120140
160180
200
Series1
Size (nm)
Fig 2
Dye combinations
600 650 700 750 800 850 900 950 10000
0.1
0.2
0.3
0.4
0.5
0.6
250 ul ONC and 125 ul ZnBNc
Wavelength
Abso
rban
ce
600 650 700 750 800 850 900 9500
0.1
0.2
0.3
0.4
0.5
0.6
250 ul ONc 250 ul ZnBNc
Wavelength
Abso
rban
ce
600 650 700 750 800 850 900 950 10000
0.10.20.30.40.50.60.70.80.9
250 ul ONC 500 ul ZnBNc
wavelength
abso
rban
ce
600 650 700 750 800 850 900 950 10000
0.10.20.30.40.50.60.70.80.9
1
500 ul onc and 250 ul znbnc
wavelength
abso
rban
ce
ZnBNc (707 nm) experiences a red shift when combined with ONc (860 nm)
860 upconverting nanoparticles with Vaseline
0 50 100 150 200 250 30030
35
40
45
50
55
60
65
70
Temperature Vs. Time Of Different O.D.
0 O.D.12.5 O.D.25 O.D.50 O.D.
Time (Seconds)
Tem
pera
ture
(Cel
cius)
Power Density = 550
0 50 100 150 200 250 30030
35
40
45
50
55
60
65
70
Temperature of Tumor
50 ul NNP100 ul NNP200 ul NNP
Time (Seconds)
Tem
pera
ture
(Cel
cius)
Tumor, 200 ul (100 OD), 0 seconds
Tumor, 200 ul (100 OD), 30 seconds
Tumor, 200 ul (100 OD), 1.5 minutes
PD= 400
20 days after PTT
100 OD injection 50 OD injection
0 10 20 30 40 50 60 70 80 90 100 110 1200
0.02
0.04
0.06
0.08
0.1
0.12
Absorbance of nanonap after injection into tumor
Injected O.D.
Abso
rban
ce
0
0.5
1
1.5
2
2.5
3
3.5x 10
4100 OD
0
0.5
1
1.5
2
2.5
3
3.5x 10
450 OD
0
0.5
1
1.5
2
2.5
3
3.5x 10
4Control, 0 OD
PAT images 24 hours after nanonaps were injected into the tail vein of mice
0 20 40 60 80 100 1200
0.1
0.2
0.3
0.4
0.5
0.6
0.7
Mass of dye in tumor
Injected OD
Mas
s of O
Nc (
mg
dye/
kg
tum
or)
0 20 40 60 80 100 1200
20
40
60
80
100
120
Measured O.D. in tumor
Injected O.D.
Mea
sure
d O
.D. a
fter t
umor
hom
ogen
izatio
n
100 OD Injected into mouse
0.5
1
1.5
2
2.5
3
3.5x 10
4
0.5
1
1.5
2
2.5
3
3.5x 10
4
At 860 nm laser At 820 nm laser