lab 5 sodium dodecyl sulfate (sds)- polyacrylamide gel...
TRANSCRIPT
Lab 5
Sodium Dodecyl Sulfate (SDS)- Polyacrylamide Gel Electrophoresis (PAGE)
Gel Electrophoresis/
Technique that relies on an electric field to separate molecules based on their size and charge
★
SDS-PAGE SDS = detergent
Polyacrylamide Gel Electrophoresis
Tertiary Structure
Quaternary Structure
Sample Buffer: SDS Bromphenol Blue Glycerol
Reducing Sample Buffer: SDS Bromphenol Blue Glycerol β-mercaptoethanol
Loading the Gel
Sample Preparation
Relative Mobility
Semi-Log Graph Paper
RM
MW
/Da
1x 103
1x 104
1x 105
1x 106
Enztme-Linked Immunosorbant Assay ELISA
Terminology
o Immunology o Antigen o Antibody
Antibodies as vital tools in scientific research, in biotechnology and in diagnosing and treating diseases
ELISA Relies on antibodies to detect foreign entities in liquid samples
Disease detection Pregnancy tests And many others…
ELISA
Immuno Sorbant
Surface/ Polystyrene
Antigen-Antibody Complex
Antibodies Recognize Specific Antigens
Constant region is not involved in antigen binding!
★