This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

la

lb

lc

ld

le

lh

lg

lf

lglf

lbla

lc

ldle

1A

1D

1H

1I

Light Chain A -1

1B

1F

1E

1C

NH

3 +

AL1

Chain A

AL2

1G

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

lhli

lj

li

lk

lj

lk

ll

ll

lmln

lm

ln

2E

Light Chain A - 2

2F

2G2A

2B

2C

COO

-

AL

Chain A

AL4

AL3

2D

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

ha

hb

hc

hd

he

hhhg

hf

hghf

hbha

hc

hdhe

1D

Heavy Chain B - 1

hh

hi

NH

3 +

1A

1C

1B

1E1F

1G1H

1I

Chain B

BH1

BH2

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

hj

hk

hl

hm

hn

hr

hq

ho

hpho

hkhj

hl

hmhn

Heavy Chain B - 2

hq

hp

hi

2A

2B

2C

2D

2F

2G

2E

Chain B

BH3

BH4

BH

BHA BHB

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

hs

ht

hu

hv

hwhz

hy

hx

hyhx

hths

hu

hvhw

Heavy Chain B - 3

hz

haa

hr

3B

3C

3F3E

3G

3D

3A

Chain B

BH5

BH6

BHC

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

hab

hac

had

hae

haf

hag

haghac

hab

had

haehaf

Heavy Chain B - 4

haa

COO

-

4A

4B

4D

4C

4F

4G

4E

Chain B

BH7

BH8

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

la

lb

lc

ld

le

lh

lg

lf

lglf

lbla

lc

ldle

1A

1D

1H

1I

Light Chain C -1

1B

1F

1E

1C

NH

3 +

CL1

Chain C

CL2

1G

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

lhli

lj

li

lk

lj

lk

ll

ll

lmln

lm

ln

2D

2E

Light Chain C - 2

2F

2G2A

2B

2C

COO

-

CL

Chain C

CL4

CL3

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

ha

hb

hc

hd

he

hhhg

hf

hghf

hbha

hc

hdhe

1D

Heavy Chain D - 1

hh

hi

NH

3 +

1A

1C

1B

1E1F

1G1H

1I

Chain D

DH1

DH2

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

hj

hk

hl

hm

hn

hr

hq

ho

hpho

hkhj

hl

hmhn

Heavy Chain D - 2

hq

hp

hi

2A

2B

2C

2D

2F

2G

2E

Chain D

DH3

DH4

DH

DHA DHB

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

hs

ht

hu

hv

hwhz

hy

hx

hyhx

hths

hu

hvhw

Heavy Chain D - 3

hz

haa

hr

3B

3C

3F3E

3G

3D

3A

Chain D

DH5

DH6

DHC

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

hab

hac

had

hae

haf

hag

haghac

hab

had

haehaf

Heavy Chain D - 4

haa

COO

-

4A

4B

4D

4C

4F

4G

4E

Chain D

DH7

DH8

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

Disulfide Linkages

AL1AL3

ALBH

5BH

7BH

1BH

3

AL2

AL4

BHBH

2BH

4BH

6BH

8

BHB D

HB

BHC D

HC

BHA D

HACL1

CL3CL

DH

5D

H7

DH

1D

H3

AL2

CL4

DH

DH

2D

H4

DH

6D

H8

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

InstructionsWork on one sheet at a time to avoid mixing up the strips. The antibody model contains 2 light chains and 2 heavy chains. There are 2 sheets for each light chain and 4 for each heavy chain. For each sheet:

1. Cut out each strip along the dotted lines:• Note that the shaded regions labeled la, lb, etc. should be included in the strip • Cut out the Disulfide Linkage strips for modeling disulfide bridges

2. Make the immunoglobulin domains: There are 2 immunoglobulin domains in each light chain (A and C) and 4 domains in each heavy chain (B and D)• Tape together the cut out strips into a long strand for each domain to represent the polymer backbone• The labels la, lb, etc. on the white background should be taped on top of corresponding labels on the shaded background• There should be 12 such long strands in all.• Assemble the immunoglobulin domain by taping together the strands side by side as you see on the picture.

Align the grey area between the strands. Make sure that the beta strand arrows point up and down alternately. Use the red labels and create clusters as follows:

1. Chain A & C: 1A–1D, 1E–1I, 2A–2D, and 2E–2G2. Chain B & D: 1A–1D, 1E–1I, 2A–2D, 2E–2G, 3A–3D, 3E–3G, 4A – 4D, 4E–4G

3. Assembling the 4 polymer chains• Tape together the 2 domains of the light chain at the label lh• Tape together the 4 domains of the heavy chain at the labels hi, hr and haa

4. Disulphide linkages• Fold down both hatched regions (marked AL1, AL2, etc.) on the strips representing the disulphide linkages.• Identify the round labels marked AL1 and AL2 on the Chain A. Place the corresponding disulphide

strip on the back of the printed side aligning the hatched regions with the round label. Repeat this for each immunoglobulin domain.

• Identify the dotted round labels AL and BH on the model and connect them with corresponding disulphide strip.Tape the printed side of the hatched regions on top of the dotted round label.

• Repeat for the strips marked CL-DH, BHA-DHA, BHB-DHB, BHC-DHC.

Suggestion: This model can be made as a group project. Assign each sheet to a different individual to cut and assemble the domain. The 12 different domains in the antibody can be assembled together and all the disulfide bridges put in place as a group.

The completed antibody model should look like the picture shown on the next slide

This activity was developed as part of the RCSB Collaborative Curriculum Development Program 2014.

Antibody (IgG):

Paper Model Computer Model (PDB ID: 1igt)