KOMP and KOMP2

Trish LaboskyScientific Co-director

International Project

KOMPKnock Out Mouse Project

Trans-NIH initiative, aims to generate a comprehensive and public resource of murine ESCs containing a null mutation in every gene in the mouse genome.

KOMP Goals1) Use gene targeting to generate null alleles,

marked with a high utility reporter, preferably in C57BL/6;

2) Develop improved C57BL/6 ES cells capable of robust germline transmission, to be used in a high throughput pipeline in generating this resource;

3) Support a repository to house the products of this resource, additional ‘repatriation' effort to bring in 1000 of the existing high priority mouse knockouts;

4) Implement a data coordination center to make the status and relevant data of the production effort available to the research community.

1. Regeneron Pharmaceuticals2. Children's Hospital Oakland Research Institute (CHORI), School of Veterinary Medicine, University of California, Davis (UC Davis) and the Wellcome Trust Sanger Institute.3. The Jackson Laboratory.

www.knockoutmouse.org

Additional KOMP miceNIH awarded contracts that gave academic researchers access to a set of well-characterized knockout mouse lines from Deltagen and Lexicon Genetics.

~ $11 million for ~250 lines of these mice

http://www.nih.gov/science/models/mouse/deltagenlexicon/list.html

http://www.nih.gov/science/models/mouse/deltagenlexicon/list.html

Nature (2011) 474, 262.

Vast majority of mice are from the Wellcome Trust

Have targeted more than 9000 genes.

Null allele

Null allele with reporter

Conditional allele

What if you don’t know which genes are involved in your biological system/question?

KOMP2: Newly Funded

HarwellJaxBaylorUC Davis

Phenotyping Platform

FeesMRRCC

KOMP Davis

What to do when you find a line of interest:

You submit MTA, order ESCs (we recommend the “PREMIUM ESC PACKAGE”, $2801), and have them sent to us.

Additional expenses:

ESC expansion, freezeback, prep for path screening $1000 / cloneESC Karyotyping (if needed)

$350 / clonePath screening by DAC

$350 / cloneESC prep / injection day

$450Injection into Albino BL6 / day

$2600Injection into BL6 / day

$1650

TMESCSR Website

http://www.vanderbiltresearch.org/community/profile/id/8