CURRICULUM VITAE (CV)

Personal Details:

Name & Surname Khaled AlTaweel

Academic Degree PhD in Functional Genomics &Genetic Engineering

Place & Date of Birth Damascus, Syria, 1/1/1972Sex MalePresent Position & Address Research Associate in the Department of

Plant Science, Faculty of Agricultural & FoodSciences University of Manitoba, Winnipeg,MB R3T2N2.Tel : (204) 474-8352 (O) : (204) 417-0613 (H)Fax : (204) 474-7528E-mail : khaledta72@hotmail.com

Academic and Technical Education:

Year Degree Average% Institution

2010/2011 Research Associate in the field of Functional Genomics -

Manitoba University, Facultyof Agricultural & Food

Sciences, Canada

2009/2010

Post-Doc in the field of Functional Genomics -

Manitoba University, Facultyof Agricultural & Food

Sciences, Canada

2004/2007

Ph. D in the field of Genetic Engendering A+

Osaka Prefecture University,Graduate School of Life &

Environmental Sciences, Japan2001/200

3M. Sc

in the field of Biotechnology 90 Faculty of Agriculture,Damascus University, Syria.

1995/1996

Diploma in the field of Crops 76.16 Faculty of Agriculture,

Damascus University, Syria.1989/199

4B. Sc

in Agricultural Engineering 61.17 Faculty of Agriculture,Damascus University, Syria.

MSc. Thesis Title: In Vitro Microtuberization of Draja and Some Introduced Varieties ofPotato using Plant Tissue Culture Techniques.

PhD. Thesis Title: A Bacterial Transgene for Catalase Protects Translation of D1 Proteinduring Exposure of Salt-Stressed Tobacco Leaves to Strong Light.

Post-Doc Project: - Molecular Identification and Characterization of Wheat Resistance Genes Expressed

under Different Isolates of Fusarium Graminearum.Research Associate Project (continuous):

- Transcriptome Profiling of Wheat Differentially Expressed Genes under DifferentIsolates of Fusarium Graminearum

Techniques Known:

1) Plant tissue culture & Plant breeding (conventional and molecular)2) Molecular characterization of plant germplasm3) DNA markers for crop improvement (RAPD, AFLP, SSR, SRAP,SCAR marker)4) Preparation of transformation constructs (vectors)5) Molecular characterization of transformants6) Plant genetic transformation & GMOs detection and evaluation.7) Plasmid DNA isolation, quantification, and restriction analysis.8) Genomic DNA isolation, quantification, and restriction analysis.9) Primer designing, PCR, RT-PCR, Gel electrophoresis, Geldocumentation, ELISA, Southern hybridization, Northern & Westernblotting.

10) Differential genes expression methods: Differential Display RT-PCR(DDRT-PCR), Suppressive Subtractive Hybridization (SSH).

11) Library construction: cDNA library synthesis, subtracted cDNA library.

Research Experiences:

1) Standardization plant tissue culture protocol2) Regeneration survey (evaluation of different plant genotypes forresponse to tissue culture)3) Bacteria culturing, selection of transformed cells, maintenance ofcultures.4) Performing DNA markers (DNA isolation, restriction enzymes,results scoring, phylodiversity tree)5) Plasmid DNA isolation, and transformation of bacteria forpreparation of transformation construct (vectors) 6) Biolistic (Bombardment) method of plant genetic transformation7) Molecular characterization of putative transgenic plants throughmolecular biology techniques (e.g., PCR, qRT-PCR, Southernhybridization, ELISA.8) Field evaluation of transgenic plants for agronomically importanttraits.9) GMOs detection by PCR-based methods & protein-based methods. 10) Transcriptome profiling and gene expression analysis.11) cDNA library construction. 12) Subtracted cDNA library construction.

Professional Training Courses:

Course Title Sponsors Date PlaceTissue Culture of Potato General Organization for Seeds

Multiplication (GOSM) 2002 Aleppo, Syria

Introductory GeneManipulation for Agriculture

Japan International CooperationAgency (JICA)/Japan

Aug. 14th – Dec. 13th,2002 Osaka, Japan

DNA Molecular MarkerTechniques for Crop

Improvement

The International Center forAgricultural Research in The

Dry Areas (ICARDA(

Sept. 7th – 18th,2003

ICARDA,Aleppo, Syria

DNA Molecular MarkerTechniques for Crop

Improvement

ICARDA, GCSAR, GEF &UNDP Project

Mar. 14th – 21st,2004

GCSARDamascus, Syria

Genetic Transformation andDNA Marker Applications for

Crop Improvement

German Agency for TechnicalCooperation (GTZ)

Mar. 2nd – 13th,2008

ICARDA,Aleppo, Syria

Participation in Congresses, Conferences & Workshops:

Title Conference/Workshop Organizers Date Place

Conference of Biotechnology 41st Science Week Supreme Council ofSciences.

Nov. 3rd – 5th, 2001 Damascus, Syria

Biosafety in AgriculturalBiotechnology in Syria. Workshop GCSAR / FAO &

ICARDA

Aug.30th – Sep.1st,200303 Damascus, Syria

Environmental Stress Inhibitsthe Repair of PhotodamagedPhotosystem II by Suppressingthe Transcription andTranslation of D1 ProteinEncoded by psbA Gene in

Tomato, (Presentation.(

Annual KansaiSymposium

Osaka PrefectureUniversity

July 22nd,2006 Osaka Prefecture

University, Japan

Translational Machinery ofpsbA Gene is Protected inTransgenic Tobacco PlantsExpressing E. coli Catalase(KatE) in Chloroplasts,

(Presentation.(

Annual Meeting

Japan Society forBioscience,

BiotechnologyAgrochemistry

(JSBBA(

Mar. 24th – 27th,2007

Tokyo Universityof Agriculture,

Japan

A Bacterial Transgene forCatalase EnhancesPhotosynthesis ProteinsTolerance againstEnvironmental Stress,(Presentation).

6 th Scientific

Conference

General Commissionfor Scientific

Agricultural Research(GCSAR(

Aug. 26th – 27th,2008

GCSAR, DoumaDamascus, Syria

Agrobacterium-MediatedTransformation of TomatoExpressing Bacterial Catalasein Chloroplasts,(Presentation).

1st International

Conference of

Agricultural

Science

Aleppo Uni., Syria&

Musel Uni., Iraq

Oct. 13th – 15th,2008

Faculty ofAgriculture,Aleppo Uni.,

Syria

Abiotic Stress Resistance inTobacco is Enhanced byOverexpression of A CatalaseEnzyme, (Presentation).

Annual MeetingCanadian

PhytopathologicalSociety (CPS)

June 22nd – 25th,2009

University ofManitoba,Manitoba,

CanadaA survey to examine a SCARmarker as a tool todifferentiate resistance andsusceptible cultivars and linesof canola against differentLeptosphaeria maculans PG3isolates, (Poster).

Annual Meeting CanadianPhytopathological

Society (CPS)

June 22nd – 25th,2009

Delta HotelWinnipeg, MB,

Canada

6th Canadian Workshopon Fusarium Head Blight

(Attendance)Workshop

Agriculture and Agri-Food Canada

(AAFC)

Nov. 1st – 4th,2009

Marriott Hotel,Ottawa, Ontario

CanadaMolecular Identification andCharacterization of WheatResistance Genes Expressedunder Different Isolates ofFusarium Graminearum,

4th Annual MeetingCanadian-GermanCollaboration onPlant Genomics

Research

Nov. 5th – 6th,2009

Agriculture andAgri-Food

Canada(AAFC/ECORC), Ottawa, Canada

(Presentation). (GABI-Canada)

Construction of subtractivecDNA library andidentification of differentiallyexpressed genes from wheatcv. Sumai-3 under Fusariumgraminearum infection,(Poster).

Joint AnnualAnnual Meetingand Conference

CanadianPhytopathological

Society (CPS)with the

Pacific Division ofthe American

PhytopathologicalSociety

June 20th – 23rd,2010

University ofBritish

Columbia,Vancouver, BC,

Canada

Differentially expressed genesof wheat cultivar Sumai3resistant to the pathogenicfungus Fusariumgraminearum, (Poster).

9th InternationalMycological

CongressInternationalMycologicalAssociation

August 1st – 6th,2010

EdinburghInternationalConferenceCentre, UK

Application of SuppressionSubtractive Hybridization(SSH) to CloningDifferentially ExpressedcDNA in wheat (Sumai3)Under Fusariumgraminearum Infection(Poster)

2010 NationalFusarium HeadBlight Forum

U.S. Wheat andBarley Scab Initiative

(USWBSI)

December 7th-9th , 2010

Hyatt RegencyMilwaukee,Milwaukee,

Wisconsin, USA

Induction of wheat defensegenes in response to Fusariumgraminearum infection,(Presentation).

Manitoba RegionalMeeting

The CanadianPhytopathological

Society (CPS)

December 9th,2010

CRC-Agriculture& Agri-Food

Canada,Winnipeg, MB.

Transcriptome Analysis of awheat cultivar infected bydifferent chemotypes ofFusarium graminearum(Poster)

Joint AnnualMeeting andConference

The AmericanPhytopathological

Society (APS)&

The InternationalAssociation for the

Plant ProtectionSciences (IPPC)

August 6th -10th 2011

Honolulu,Hawaii, USA

Transcriptome Profiling ofDifferentially ExpressedGenes of Wheat after Infectionwith Different F.graminearum Chemotypes(Presentation).

7th CanadianWorkshop

on Fusarium HeadBlight

http://www.cwfhb.org/Program%20-

%20CWFHB%207.pdf

Agriculture and Agri-Food Canada

(AAFC)

Nov. 27th

– 30th, 2011

Delta HotelWinnipeg, MB,

Canada

Are wheat genes expresseddifferentially when infected bydifferent F. graminearum Chemotypes, (Presentation).

Manitoba RegionalMeeting

http://phytopath.ca/newsletter/news_v56-1.pdf

The CanadianPhytopathological

Society (CPS)

December 8th,2011

CRC-Agriculture& Agri-Food

Canada,Winnipeg, MB.

Comparative gene expressionanalysis of susceptible andresistant Wheat infected byFusarium graminearum.(Presentation).

Annual Meeting

The CanadianPhytopathological

Society (CPS)With the International

Plum Pox Virus

June 24th-27th,2012

Marriott Hotel,Niagara Falls,

Ontario, Canada

List of Publications

Khaled Al-Taweel, Al-Maarri Khaleel, Kheeti Mamoun, Abdul-Kader Ahmad (2004)Effects of Some Factors Influencing on in vitro Tuberization of Potato cv. “Draga”.Damascus University Journal for the Agricultural Sciences 20: 265-280

Khaled Al-Taweel, Toshio Iwaki, Yukinori Yabuta, Shigeru Shigeoka, Norio Murata,and Akira Wadano (2007) A Bacterial Transgene for Catalase Protects Translation ofD1 Protein during Exposure of Salt-Stressed Tobacco Leaves to Strong Light. PlantPhysiology 145: 258-265

Toshio Iwaki, Kazunori Shiota, Khaled Al-Taweel, Daisuke Kobayashi, AtsushiKobayashi, Kensaku Suzuki, Toshifumi Yui, Akira Wadano (2008) Inhibition ofRuBisCO Cloned from Thermosynechococcus vulcanus and Expressed inEsherichia coli with Compounds Predicted by Molecular Operation Environment(MOE). J. Bioscinece Bioengineering 105: 26-33

Khaled Al-Taweel, Dilantha Fernando, Anita Brule-Babel (2011) Construction andCharacterization of a cDNA Library from Wheat Infected with Fusariumgraminearum Fg 2. International Journal of Molecular Sciences 12: 613-626

Khaled Al-Taweel, Dilantha Fernando (2011) Differential Gene Expression is aPromising Tool for Understanding Host–Pathogen Interactions. In: Al-Mughrabi K(Ed) Plant science and biotechnology in North America: Focus on Canada II. TheAmericas Journal of Plant Science and Biotechnology 5 (Special Issue 1), 1-10.

Abstracts in Journals

Al-Taweel, K., Chen, X., Parks, P., Ghanbarnia, K., Dusabenyagasani, M., andFernando, W.G.D. (2009) A survey to examine a SCAR marker as a tool todifferentiate resistance and susceptible cultivars and lines of canola against differentLeptosphaeria maculans PG3 isolates. Canadian J Plant Pathology 31: 475 – 504

Al-Taweel, K., Fernando, W.G.D., and Wadano, A. (2009) Abiotic stress resistance intobacco is enhanced over expression of a catalase enzyme. Canadian J PlantPathology 31: 475 – 504

Al-Taweel, K., Guo, X., Fernando, W.G.D., and Brûlé-Babel, A. (2010) Construction ofsubtractive cDNA library and identification of differentially expressed genes fromwheat cv. Sumai-3 under Fusarium graminearum infection. Canadian J PlantPathology. In press

Al-Taweel, K., Brule-Babel, A., and Fernando, W.G.D. (2010) Induction of wheatdefense genes in response to Fusarium graminearum infection. Canadian J PlantPathology. In press.

Al-Taweel, K., Brule-Babel, A., and Fernando, W.G.D. (2011) Transcriptome Analysisof a wheat cultivar infected by different chemotypes of Fusarium graminearum. TheAmerican Phytopathological Society (APS) & The International Association for thePlant Protection Sciences (IPPC). Honolulu, Hawaii, USA.

Al-Taweel, K., Brule-Babel, A., and Fernando, W.G.D. (2011) Transcriptome profilingof differentially expressed genes of wheat after infection with different Fusariumgraminearum chemotypes.7th Canadian Workshop on Fusarium Head Blight.Winnipeg, MB, Canada. . "In press”.

Al-Taweel, K., Brule-Babel, A., and Fernando, W.G.D. (2011) Are wheat genesexpressed differentially when infected by different F.graminearum Chemotypes? Canadian J Plant Pathology. 56: 6.

Papers in Symposia proceedings at international/national meetings:

Al-Taweel, K., Brule-Babel, A.L., and Fernando, W.G.D. 2009. Molecular Identificationand Characterization of Wheat Resistance Genes Expressed under Different Isolatesof Fusarium Graminearum , 3rd Annual Canadian-German Collaboration on PlantGenomics Research, (GABI-Canada), Agriculture and Agri-Food Canada(AAFC/ECORC), Ottawa, Canada, Nov. 5th – 6th,